Journal: bioRxiv
Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis
doi: 10.1101/2025.01.10.632378
Figure Lengend Snippet: ( A ) Representative images of microvascular networks (endothelial cells in green) perfused with fluorescent dextran to indicate vessel lumens (red) in samples with fibroblasts or myofibroblasts and treated with vehicle or select compounds. Quantification of ( B ) number of vessel branches (P=0.0009 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.002 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF), ( C ) total length of vasculature, (P=0.001 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.01 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542; P=0.003 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF) ( D ) average vessel diameter (P=0.0002 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P<0.0001 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542), and ( E ) vascular permeability (P=0.006 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.0004 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542; P=0.04 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF) of microvascular networks with fibroblasts or myofibroblasts and treated with vehicle or anti-fibrotic drug. n=9-11 devices from 2 independent experiments in each group. Reported P values are based on one-way ANOVA tests with multiple comparisons.
Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).
Techniques: Permeability