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primary human lung fibroblasts  (ATCC)


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    Structured Review

    ATCC primary human lung fibroblasts
    Primary Human Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human lung fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    primary human lung fibroblasts - by Bioz Stars, 2025-02
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    Image Search Results


    Cells/cell line/cell culture medium.

    Journal: Journal of Tissue Engineering

    Article Title: Developing human upper, lower, and deep lung airway models: Combining different scaffolds and developing complex co-cultures

    doi: 10.1177/20417314241299076

    Figure Lengend Snippet: Cells/cell line/cell culture medium.

    Article Snippet: Human primary lung biopsy fibroblasts (LbFb) , Endothelial Cell Growth medium MV (Promocell, C-22120) supplemented with Endothelial supplement mix for growth medium MV (Promocell, C-39225). , Lung resections from University Hospital Magdeburg. Ethical vote 163/17. (Isolation briefly explained below).

    Techniques: Isolation

    Summarized co-culture models methods established on different synthetic scaffold varits as specified in the second column above. The summarized figure provides concise representation of the 4 types co-culture models with specific coating strategies (column 3); details on cell seeding number, seeding locations and time-point (column 4); respective co-culture medium composition (column 5); and time-point for the start of ALI culture (column 6); for respective co-cutures of (A)huAEC - hEC, (B) huAEC - LbFb, (C) huAEC-LbFb-hEC Mattek-like approach and (D) huAEC-hEC-LbFb. Figure is partly Created in BioRender. Murkar, R. (2024) https://BioRender.com/t06a312 License: VQ27JPYHHX.

    Journal: Journal of Tissue Engineering

    Article Title: Developing human upper, lower, and deep lung airway models: Combining different scaffolds and developing complex co-cultures

    doi: 10.1177/20417314241299076

    Figure Lengend Snippet: Summarized co-culture models methods established on different synthetic scaffold varits as specified in the second column above. The summarized figure provides concise representation of the 4 types co-culture models with specific coating strategies (column 3); details on cell seeding number, seeding locations and time-point (column 4); respective co-culture medium composition (column 5); and time-point for the start of ALI culture (column 6); for respective co-cutures of (A)huAEC - hEC, (B) huAEC - LbFb, (C) huAEC-LbFb-hEC Mattek-like approach and (D) huAEC-hEC-LbFb. Figure is partly Created in BioRender. Murkar, R. (2024) https://BioRender.com/t06a312 License: VQ27JPYHHX.

    Article Snippet: Human primary lung biopsy fibroblasts (LbFb) , Endothelial Cell Growth medium MV (Promocell, C-22120) supplemented with Endothelial supplement mix for growth medium MV (Promocell, C-39225). , Lung resections from University Hospital Magdeburg. Ethical vote 163/17. (Isolation briefly explained below).

    Techniques: Co-Culture Assay

    Characterization of triple cultured tissue models (huAEC-hEC-LbFb) on PCL:PTMC 50:50 (a–c) and PCL:PTMC 70:30 (d). (a) positive CD31 staining against hEC aligned below CD31-negative huAEC. (b) Alcian blue staining for models on PCL:PTMC 50:50 showing distinctive blue staining on top of multilayered epithelial layer at end of day 12, while endothelial cells were migrating inside (a–c). (c) IF staining, showing green stained CD31-positive hECs, blue DAPI stained nucleus and red E-cadherin-positive huAEC layers on top of PCL:PTMC 50:50. (d) CD31 positively stained hEC layered on the basal side of the PCL:PTMC 70:30 membrane.

    Journal: Journal of Tissue Engineering

    Article Title: Developing human upper, lower, and deep lung airway models: Combining different scaffolds and developing complex co-cultures

    doi: 10.1177/20417314241299076

    Figure Lengend Snippet: Characterization of triple cultured tissue models (huAEC-hEC-LbFb) on PCL:PTMC 50:50 (a–c) and PCL:PTMC 70:30 (d). (a) positive CD31 staining against hEC aligned below CD31-negative huAEC. (b) Alcian blue staining for models on PCL:PTMC 50:50 showing distinctive blue staining on top of multilayered epithelial layer at end of day 12, while endothelial cells were migrating inside (a–c). (c) IF staining, showing green stained CD31-positive hECs, blue DAPI stained nucleus and red E-cadherin-positive huAEC layers on top of PCL:PTMC 50:50. (d) CD31 positively stained hEC layered on the basal side of the PCL:PTMC 70:30 membrane.

    Article Snippet: Human primary lung biopsy fibroblasts (LbFb) , Endothelial Cell Growth medium MV (Promocell, C-22120) supplemented with Endothelial supplement mix for growth medium MV (Promocell, C-39225). , Lung resections from University Hospital Magdeburg. Ethical vote 163/17. (Isolation briefly explained below).

    Techniques: Cell Culture, Staining, Membrane

    Summarized comparison of tissue models on synthetic scaffold materials results summarized for different co-cultures established on synthetic scaffold variants namely PET, PCL :PTMC 50:50 and 70:30 membrane mixtures. Figure is partly Created in BioRender. Murkar, R. (2024) https://BioRender.com/t06a312 License: VQ27JPYHHX. (A-D): illustrations of the different co-cultures setup using (A) huAEC-hEC, (B) huAEC-LbFb, (C) huAEC-LbFb-hEC, (D) huAEC-hEC-LbFb, seeded as per written chronology. Preferred material composition for ECM scaffold for alveolar membranes. Detailed figures can be found in Supplemental Figures 4–5 .

    Journal: Journal of Tissue Engineering

    Article Title: Developing human upper, lower, and deep lung airway models: Combining different scaffolds and developing complex co-cultures

    doi: 10.1177/20417314241299076

    Figure Lengend Snippet: Summarized comparison of tissue models on synthetic scaffold materials results summarized for different co-cultures established on synthetic scaffold variants namely PET, PCL :PTMC 50:50 and 70:30 membrane mixtures. Figure is partly Created in BioRender. Murkar, R. (2024) https://BioRender.com/t06a312 License: VQ27JPYHHX. (A-D): illustrations of the different co-cultures setup using (A) huAEC-hEC, (B) huAEC-LbFb, (C) huAEC-LbFb-hEC, (D) huAEC-hEC-LbFb, seeded as per written chronology. Preferred material composition for ECM scaffold for alveolar membranes. Detailed figures can be found in Supplemental Figures 4–5 .

    Article Snippet: Human primary lung biopsy fibroblasts (LbFb) , Endothelial Cell Growth medium MV (Promocell, C-22120) supplemented with Endothelial supplement mix for growth medium MV (Promocell, C-39225). , Lung resections from University Hospital Magdeburg. Ethical vote 163/17. (Isolation briefly explained below).

    Techniques: Comparison, Membrane

    Scheme illustrating culture of fibroblasts or myofibroblasts on a 2D surface or in 3D, suspended in a hydrogel within microfluidic chips. Endothelial cells were co-cultured with fibroblasts or myofibroblasts in 3D to study angiogenesis and vasculogenesis of microvascular networks. The experimental timeline indicates the duration and timing of cell culture, TGF-β treatment, and assays.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: Scheme illustrating culture of fibroblasts or myofibroblasts on a 2D surface or in 3D, suspended in a hydrogel within microfluidic chips. Endothelial cells were co-cultured with fibroblasts or myofibroblasts in 3D to study angiogenesis and vasculogenesis of microvascular networks. The experimental timeline indicates the duration and timing of cell culture, TGF-β treatment, and assays.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Cell Culture

    ( A ) Fibroblasts were cultured in 2D and treated with TGF-β for 10 days. ( B ) Expression of gene transcripts in cells after treatment with TGF-β for 10 days. Expression levels are relative to untreated controls. n=3 independent plates for each group. ( C ) Representative images of F-actin (red) and α-SMA (green) in control and TGF-β-treated cells. Quantification of mean fluorescence ( D ) actin (P=0.002) and ( E ) α-SMA signal (P=0.17) in control and TGF-β-treated cells. ( F ) Representative images of collagen I (magenta) in control and TGF-β-treated cells. ( G ) Quantification of mean fluorescence collagen I signal (P=0.002). ( H ) Representative images of fibronectin (white) in control and TGF-β-treated cells. ( I ) Quantification of mean fluorescence fibronectin signal (P=0.0006). n=3-6 coverslips from 3 independent experiments for each group in D, E, G, and I. Reported P values are based on unpaired t-tests.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: ( A ) Fibroblasts were cultured in 2D and treated with TGF-β for 10 days. ( B ) Expression of gene transcripts in cells after treatment with TGF-β for 10 days. Expression levels are relative to untreated controls. n=3 independent plates for each group. ( C ) Representative images of F-actin (red) and α-SMA (green) in control and TGF-β-treated cells. Quantification of mean fluorescence ( D ) actin (P=0.002) and ( E ) α-SMA signal (P=0.17) in control and TGF-β-treated cells. ( F ) Representative images of collagen I (magenta) in control and TGF-β-treated cells. ( G ) Quantification of mean fluorescence collagen I signal (P=0.002). ( H ) Representative images of fibronectin (white) in control and TGF-β-treated cells. ( I ) Quantification of mean fluorescence fibronectin signal (P=0.0006). n=3-6 coverslips from 3 independent experiments for each group in D, E, G, and I. Reported P values are based on unpaired t-tests.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Cell Culture, Expressing, Control, Fluorescence

    ( A ) Fibroblasts or myofibroblasts were cultured in 3D within fibrin gels housed in microfluidic devices. ( B ) Expression of gene transcripts in fibroblasts or myofibroblasts after 7 days of culture in 3D. Expression levels are relative to fibroblasts. n=6 pooled devices for each group. ( C ) Representative images of F-actin (red) and α-SMA (green) in fibroblasts and myofibroblasts. Quantification of mean fluorescence ( D ) actin (P=0.003) and ( E ) α-SMA (P=0.02) signal in fibroblasts and myofibroblasts. ( F ) Representative images of collagen I in fibroblasts and myofibroblasts. ( G ) Quantification of mean fluorescence collagen I signal (P=0.20). ( H ) Representative images of fibronectin (white) in fibroblasts and myofibroblasts ( I ) Quantification of mean fluorescence fibronectin signal (P=0.16). n=6 devices from 3 independent experiments for each group in D, E, G, and I. Reported P values are based on unpaired t-tests.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: ( A ) Fibroblasts or myofibroblasts were cultured in 3D within fibrin gels housed in microfluidic devices. ( B ) Expression of gene transcripts in fibroblasts or myofibroblasts after 7 days of culture in 3D. Expression levels are relative to fibroblasts. n=6 pooled devices for each group. ( C ) Representative images of F-actin (red) and α-SMA (green) in fibroblasts and myofibroblasts. Quantification of mean fluorescence ( D ) actin (P=0.003) and ( E ) α-SMA (P=0.02) signal in fibroblasts and myofibroblasts. ( F ) Representative images of collagen I in fibroblasts and myofibroblasts. ( G ) Quantification of mean fluorescence collagen I signal (P=0.20). ( H ) Representative images of fibronectin (white) in fibroblasts and myofibroblasts ( I ) Quantification of mean fluorescence fibronectin signal (P=0.16). n=6 devices from 3 independent experiments for each group in D, E, G, and I. Reported P values are based on unpaired t-tests.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Cell Culture, Expressing, Fluorescence

    ( A ) Endothelial cells were co-cultured with either fibroblasts or myofibroblasts in 3D for angiogenesis analysis. ( B ) Representative images of endothelial cell sprouts (green) co-cultured with fibroblasts or myofibroblasts. ( C ) Endothelial sprout area in cultures with fibroblasts or myofibroblasts (P<0.0001, unpaired t-test). n=12 devices from 3 independent experiments for each group.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: ( A ) Endothelial cells were co-cultured with either fibroblasts or myofibroblasts in 3D for angiogenesis analysis. ( B ) Representative images of endothelial cell sprouts (green) co-cultured with fibroblasts or myofibroblasts. ( C ) Endothelial sprout area in cultures with fibroblasts or myofibroblasts (P<0.0001, unpaired t-test). n=12 devices from 3 independent experiments for each group.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Cell Culture

    ( A ) Microvascular networks were formed with endothelial cells and either fibroblasts or myofibroblasts. ( B ) Representative images of microvascular networks (endothelial cells in green) perfused with fluorescent dextran to indicate vessel lumens (red) in samples with fibroblasts or myofibroblasts. Quantification of ( C ) number of vessel branches (P=0.18), ( D ) total length of vasculature (P=0.06), ( E ) average vessel diameter (P=0.0002), and ( F ) vascular permeability (P<0.0001, Mann-Whitney U test) of microvascular networks with fibroblasts or myofibroblasts. n=16 devices from 3 independent experiments in each group. Reported P values for all measurements other than permeability are based on unpaired t-tests.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: ( A ) Microvascular networks were formed with endothelial cells and either fibroblasts or myofibroblasts. ( B ) Representative images of microvascular networks (endothelial cells in green) perfused with fluorescent dextran to indicate vessel lumens (red) in samples with fibroblasts or myofibroblasts. Quantification of ( C ) number of vessel branches (P=0.18), ( D ) total length of vasculature (P=0.06), ( E ) average vessel diameter (P=0.0002), and ( F ) vascular permeability (P<0.0001, Mann-Whitney U test) of microvascular networks with fibroblasts or myofibroblasts. n=16 devices from 3 independent experiments in each group. Reported P values for all measurements other than permeability are based on unpaired t-tests.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Permeability, MANN-WHITNEY

    ( A ) TGF-β1 secreted in 3D monocultures of fibroblasts or myofibroblasts or in microvascular networks containing either fibroblasts or myofibroblasts. ( B ) Panel of cytokines secreted in 3D monocultures of fibroblasts or myofibroblasts. ( C ) Panel of cytokines secreted by microvascular networks containing either fibroblasts or myofibroblasts cultured in 3D. n=6 pooled devices per group.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: ( A ) TGF-β1 secreted in 3D monocultures of fibroblasts or myofibroblasts or in microvascular networks containing either fibroblasts or myofibroblasts. ( B ) Panel of cytokines secreted in 3D monocultures of fibroblasts or myofibroblasts. ( C ) Panel of cytokines secreted by microvascular networks containing either fibroblasts or myofibroblasts cultured in 3D. n=6 pooled devices per group.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Cell Culture

    ( A ) Representative images of microvascular networks (endothelial cells in green) perfused with fluorescent dextran to indicate vessel lumens (red) in samples with fibroblasts or myofibroblasts and treated with vehicle or select compounds. Quantification of ( B ) number of vessel branches (P=0.0009 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.002 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF), ( C ) total length of vasculature, (P=0.001 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.01 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542; P=0.003 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF) ( D ) average vessel diameter (P=0.0002 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P<0.0001 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542), and ( E ) vascular permeability (P=0.006 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.0004 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542; P=0.04 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF) of microvascular networks with fibroblasts or myofibroblasts and treated with vehicle or anti-fibrotic drug. n=9-11 devices from 2 independent experiments in each group. Reported P values are based on one-way ANOVA tests with multiple comparisons.

    Journal: bioRxiv

    Article Title: Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized microphysiological model of lung fibrosis

    doi: 10.1101/2025.01.10.632378

    Figure Lengend Snippet: ( A ) Representative images of microvascular networks (endothelial cells in green) perfused with fluorescent dextran to indicate vessel lumens (red) in samples with fibroblasts or myofibroblasts and treated with vehicle or select compounds. Quantification of ( B ) number of vessel branches (P=0.0009 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.002 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF), ( C ) total length of vasculature, (P=0.001 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.01 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542; P=0.003 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF) ( D ) average vessel diameter (P=0.0002 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P<0.0001 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542), and ( E ) vascular permeability (P=0.006 for Fibroblasts, Vehicle vs. Myofibroblasts, Vehicle; P=0.0004 for Myofibroblasts, Vehicle vs. Myofibroblasts, SB 431542; P=0.04 for Myofibroblasts, Vehicle vs. Myofibroblasts, VEGF) of microvascular networks with fibroblasts or myofibroblasts and treated with vehicle or anti-fibrotic drug. n=9-11 devices from 2 independent experiments in each group. Reported P values are based on one-way ANOVA tests with multiple comparisons.

    Article Snippet: Primary human lung fibroblasts (Lonza, CC-2512, passage 8) were thawed and seeded in two 75 cm 2 flasks in Fibrolife S2 medium (Lifeline Cell Technology, LL-0011).

    Techniques: Permeability