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Santa Cruz Biotechnology primary antibodies for trim37
mRNA and protein expression levels of <t>TRIM37</t> are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.
Primary Antibodies For Trim37, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies for trim37/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
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1) Product Images from "TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer"

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2017.6125

mRNA and protein expression levels of TRIM37 are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.
Figure Legend Snippet: mRNA and protein expression levels of TRIM37 are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Western Blot

TRIM37 promotes the proliferation, migration and invasion abilities in CRC cells. (A) TRIM37-KD in SW620 cells inhibited cell proliferation, whereas TRIM37-OV in SW480 cells increased proliferation. *P<0.05. (B) Crystal violet assay was used to analyze the effects of TRIM37 on the growth of CRC cells. (C) Representative images of the wound healing assay of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. (D) Representative images of the Transwell assays of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. Data are representative of three independent experiments. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression.
Figure Legend Snippet: TRIM37 promotes the proliferation, migration and invasion abilities in CRC cells. (A) TRIM37-KD in SW620 cells inhibited cell proliferation, whereas TRIM37-OV in SW480 cells increased proliferation. *P<0.05. (B) Crystal violet assay was used to analyze the effects of TRIM37 on the growth of CRC cells. (C) Representative images of the wound healing assay of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. (D) Representative images of the Transwell assays of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. Data are representative of three independent experiments. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression.

Techniques Used: Migration, Crystal Violet Assay, Wound Healing Assay, Over Expression

TRIM37 promotes the invasion of colorectal cancer cells via EMT. (A) Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of several components that were involved in EMT. In the TRIM37-KD SW620 cells, N-CAD and VIM were downregulated, whereas E-CAD was upregulated. *P<0.05. (B) In the TRIM37-OV SW480 cells, N-CAD and VIM were upregulated, whereas E-CAD was downregulated. *P<0.05. (C) Western blot analysis demonstrated that, in TRIM37-KD SW620 cells, the epithelial marker E-CAD was upregulated, whereas N-CAD and VIM, the mesenchymal markers, were downregulated. (D) In TRIM37-OV SW480 cells, E-CAD was downregulated, whereas N-CAD and VIM were upregulated. TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression; E-CAD, E-cadherin; N-CAD, N-cadherin; VIM, vimentin.
Figure Legend Snippet: TRIM37 promotes the invasion of colorectal cancer cells via EMT. (A) Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of several components that were involved in EMT. In the TRIM37-KD SW620 cells, N-CAD and VIM were downregulated, whereas E-CAD was upregulated. *P<0.05. (B) In the TRIM37-OV SW480 cells, N-CAD and VIM were upregulated, whereas E-CAD was downregulated. *P<0.05. (C) Western blot analysis demonstrated that, in TRIM37-KD SW620 cells, the epithelial marker E-CAD was upregulated, whereas N-CAD and VIM, the mesenchymal markers, were downregulated. (D) In TRIM37-OV SW480 cells, E-CAD was downregulated, whereas N-CAD and VIM were upregulated. TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression; E-CAD, E-cadherin; N-CAD, N-cadherin; VIM, vimentin.

Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Marker, Over Expression



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Abcam primary antibody against trim37
Relative <t>TRIM37</t> expression in GC tissues and its clinical significances. Notes: ( A , B ) qRT-PCR analysis of TRIM37 mRNA expression in GC tumor tissues and paracancerous control tissues. ( C ) Western blot analysis of TRIM37 protein expression in GC tumor tissues and paracancerous control tissues. ( D ) Representative IHC images of TRIM37 protein expression in clinical GC samples with or without metastasis and paracancerous control tissues. ( E ) Quantitative evaluation of TRIM37 protein expression in tumor tissues and paracancerous control tissues on the basis of staining scores. ( F ) Scatterplots of the average staining scores of TRIM37 expression in patients without or with metastasis. ( G ) Kaplan–Meier analysis of the correlation between TRIM37 expression and the overall survival of patients with GC. * P <0.05. Abbreviations: GC, gastric cancer; IHC, immunohistochemistry; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TRIM37, tripartite motif containing 37.
Primary Antibody Against Trim37, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson trim37 primary antibody
Relative <t>TRIM37</t> expression in GC tissues and its clinical significances. Notes: ( A , B ) qRT-PCR analysis of TRIM37 mRNA expression in GC tumor tissues and paracancerous control tissues. ( C ) Western blot analysis of TRIM37 protein expression in GC tumor tissues and paracancerous control tissues. ( D ) Representative IHC images of TRIM37 protein expression in clinical GC samples with or without metastasis and paracancerous control tissues. ( E ) Quantitative evaluation of TRIM37 protein expression in tumor tissues and paracancerous control tissues on the basis of staining scores. ( F ) Scatterplots of the average staining scores of TRIM37 expression in patients without or with metastasis. ( G ) Kaplan–Meier analysis of the correlation between TRIM37 expression and the overall survival of patients with GC. * P <0.05. Abbreviations: GC, gastric cancer; IHC, immunohistochemistry; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TRIM37, tripartite motif containing 37.
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Santa Cruz Biotechnology primary antibodies for trim37
mRNA and protein expression levels of <t>TRIM37</t> are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.
Primary Antibodies For Trim37, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies for trim37/product/Santa Cruz Biotechnology
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Santa Cruz Biotechnology trim37 primary antibody
mRNA and protein expression levels of <t>TRIM37</t> are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.
Trim37 Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trim37 primary antibody/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
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Image Search Results


Relative TRIM37 expression in GC tissues and its clinical significances. Notes: ( A , B ) qRT-PCR analysis of TRIM37 mRNA expression in GC tumor tissues and paracancerous control tissues. ( C ) Western blot analysis of TRIM37 protein expression in GC tumor tissues and paracancerous control tissues. ( D ) Representative IHC images of TRIM37 protein expression in clinical GC samples with or without metastasis and paracancerous control tissues. ( E ) Quantitative evaluation of TRIM37 protein expression in tumor tissues and paracancerous control tissues on the basis of staining scores. ( F ) Scatterplots of the average staining scores of TRIM37 expression in patients without or with metastasis. ( G ) Kaplan–Meier analysis of the correlation between TRIM37 expression and the overall survival of patients with GC. * P <0.05. Abbreviations: GC, gastric cancer; IHC, immunohistochemistry; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TRIM37, tripartite motif containing 37.

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: Relative TRIM37 expression in GC tissues and its clinical significances. Notes: ( A , B ) qRT-PCR analysis of TRIM37 mRNA expression in GC tumor tissues and paracancerous control tissues. ( C ) Western blot analysis of TRIM37 protein expression in GC tumor tissues and paracancerous control tissues. ( D ) Representative IHC images of TRIM37 protein expression in clinical GC samples with or without metastasis and paracancerous control tissues. ( E ) Quantitative evaluation of TRIM37 protein expression in tumor tissues and paracancerous control tissues on the basis of staining scores. ( F ) Scatterplots of the average staining scores of TRIM37 expression in patients without or with metastasis. ( G ) Kaplan–Meier analysis of the correlation between TRIM37 expression and the overall survival of patients with GC. * P <0.05. Abbreviations: GC, gastric cancer; IHC, immunohistochemistry; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TRIM37, tripartite motif containing 37.

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Staining, Immunohistochemistry, Reverse Transcription Polymerase Chain Reaction

Correlation with the clinicopathological features and  TRIM37  expression in patients with GC

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: Correlation with the clinicopathological features and TRIM37 expression in patients with GC

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Expressing

Relative TRIM37 protein expressions in GC cells. Notes: ( A ) Western blot analysis of relative TRIM37 expression in GC cell lines and normal gastric epithelial GES-1 cells. ( B , C ) Western blot analysis of TRIM37 expression levels in TRIM37-silencing cells (MKN45 and MGC803) and TRIM37-overexpressing cells (KATO-III and AGS). * P <0.05. Abbreviations: GC, gastric cancer; TRIM37, tripartite motif containing 37.

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: Relative TRIM37 protein expressions in GC cells. Notes: ( A ) Western blot analysis of relative TRIM37 expression in GC cell lines and normal gastric epithelial GES-1 cells. ( B , C ) Western blot analysis of TRIM37 expression levels in TRIM37-silencing cells (MKN45 and MGC803) and TRIM37-overexpressing cells (KATO-III and AGS). * P <0.05. Abbreviations: GC, gastric cancer; TRIM37, tripartite motif containing 37.

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Western Blot, Expressing

Effects of TRIM37 knockdown or overexpression on GC cells migration, invasion in vitro, and metastasis in vivo. Notes: ( A ) Representative images of the effects of TRIM37 knockdown or overexpression on the morphology of GC cells. ( B ) The influences of TRIM37 silencing or overexpression on the migration and invasion abilities of GC cells were measured by transwell assay. ( C ) The effects of TRIM37 silencing or overexpression on GC cells metastasis in vivo. Metastatic tumor nodules (shown by red arrows). ( D ) Western blot analysis of expressions of TRIM37, E-cadherin, SIP1, and N-cadherin in lung metastatic nodules. * P <0.05. Abbreviations: GC, gastric cancer; TRIM37, tripartite motif containing 37.

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: Effects of TRIM37 knockdown or overexpression on GC cells migration, invasion in vitro, and metastasis in vivo. Notes: ( A ) Representative images of the effects of TRIM37 knockdown or overexpression on the morphology of GC cells. ( B ) The influences of TRIM37 silencing or overexpression on the migration and invasion abilities of GC cells were measured by transwell assay. ( C ) The effects of TRIM37 silencing or overexpression on GC cells metastasis in vivo. Metastatic tumor nodules (shown by red arrows). ( D ) Western blot analysis of expressions of TRIM37, E-cadherin, SIP1, and N-cadherin in lung metastatic nodules. * P <0.05. Abbreviations: GC, gastric cancer; TRIM37, tripartite motif containing 37.

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Over Expression, Migration, In Vitro, In Vivo, Transwell Assay, Western Blot

Effects of TRIM37 knockdown or overexpression on EMT-related markers in GC cells. Notes: Following TRIM37 silencing or overexpression treatment, qRT-PCR ( A ), Western blot ( B ), and immunofluorescence ( C ) were employed to detect the expressions of EMT-related markers in TRIM37-altered cells, including E-cadherin, N-cadherin, and transcription factors (SIP1, Snail, Slug, ZEB1, and Twist). * P <0.05. Abbreviations: EMT, epithelial–mesenchymal transition; GC, gastric cancer; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TRIM37, tripartite motif containing 37.

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: Effects of TRIM37 knockdown or overexpression on EMT-related markers in GC cells. Notes: Following TRIM37 silencing or overexpression treatment, qRT-PCR ( A ), Western blot ( B ), and immunofluorescence ( C ) were employed to detect the expressions of EMT-related markers in TRIM37-altered cells, including E-cadherin, N-cadherin, and transcription factors (SIP1, Snail, Slug, ZEB1, and Twist). * P <0.05. Abbreviations: EMT, epithelial–mesenchymal transition; GC, gastric cancer; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TRIM37, tripartite motif containing 37.

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Immunofluorescence, Reverse Transcription Polymerase Chain Reaction

SIP1-dependent mechanism of TRIM37-facilitated GC cells EMT and invasion. Notes: ( A , C ) Western blot analysis of the SIP1-dependent mechanism of TRIM37-inducing GC cells EMT. ( B , D ) Confirmation of the SIP1-dependent mechanism of TRIM37-inducing GC cells invasion by transwell assay. * P <0.05. Abbreviations: EMT, epithelial–mesenchymal transition; GC, gastric cancer; TRIM37, tripartite motif containing 37.

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: SIP1-dependent mechanism of TRIM37-facilitated GC cells EMT and invasion. Notes: ( A , C ) Western blot analysis of the SIP1-dependent mechanism of TRIM37-inducing GC cells EMT. ( B , D ) Confirmation of the SIP1-dependent mechanism of TRIM37-inducing GC cells invasion by transwell assay. * P <0.05. Abbreviations: EMT, epithelial–mesenchymal transition; GC, gastric cancer; TRIM37, tripartite motif containing 37.

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Western Blot, Transwell Assay

Primers designed for qRT-PCR

Journal: OncoTargets and therapy

Article Title: TRIM37 promotes cell invasion and metastasis by regulating SIP1-mediated epithelial–mesenchymal transition in gastric cancer

doi: 10.2147/OTT.S178446

Figure Lengend Snippet: Primers designed for qRT-PCR

Article Snippet: Primary antibody against TRIM37 was purchased from Abcam (Cambridge, UK).

Techniques: Sequencing

mRNA and protein expression levels of TRIM37 are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.

Journal: Molecular Medicine Reports

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

doi: 10.3892/mmr.2017.6125

Figure Lengend Snippet: mRNA and protein expression levels of TRIM37 are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.

Article Snippet: Primary antibodies for TRIM37 (1:500; cat. no. sc-49548, Santa Cruz Biotechnology, Inc.), GAPDH (1:1,000; cat. no. sc-293335, Santa Cruz Biotechnology, Inc.), E-cadherin (1:500; cat. no. ab76055, Abcam, Cambridge, UK), N-cadherin (1:500; cat. no. ab12221, Abcam) and vimentin (1;500; cat. no. ab72547, Abcam) were incubated overnight at 4°C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Western Blot

TRIM37 promotes the proliferation, migration and invasion abilities in CRC cells. (A) TRIM37-KD in SW620 cells inhibited cell proliferation, whereas TRIM37-OV in SW480 cells increased proliferation. *P<0.05. (B) Crystal violet assay was used to analyze the effects of TRIM37 on the growth of CRC cells. (C) Representative images of the wound healing assay of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. (D) Representative images of the Transwell assays of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. Data are representative of three independent experiments. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression.

Journal: Molecular Medicine Reports

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

doi: 10.3892/mmr.2017.6125

Figure Lengend Snippet: TRIM37 promotes the proliferation, migration and invasion abilities in CRC cells. (A) TRIM37-KD in SW620 cells inhibited cell proliferation, whereas TRIM37-OV in SW480 cells increased proliferation. *P<0.05. (B) Crystal violet assay was used to analyze the effects of TRIM37 on the growth of CRC cells. (C) Representative images of the wound healing assay of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. (D) Representative images of the Transwell assays of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. Data are representative of three independent experiments. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression.

Article Snippet: Primary antibodies for TRIM37 (1:500; cat. no. sc-49548, Santa Cruz Biotechnology, Inc.), GAPDH (1:1,000; cat. no. sc-293335, Santa Cruz Biotechnology, Inc.), E-cadherin (1:500; cat. no. ab76055, Abcam, Cambridge, UK), N-cadherin (1:500; cat. no. ab12221, Abcam) and vimentin (1;500; cat. no. ab72547, Abcam) were incubated overnight at 4°C.

Techniques: Migration, Crystal Violet Assay, Wound Healing Assay, Over Expression

TRIM37 promotes the invasion of colorectal cancer cells via EMT. (A) Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of several components that were involved in EMT. In the TRIM37-KD SW620 cells, N-CAD and VIM were downregulated, whereas E-CAD was upregulated. *P<0.05. (B) In the TRIM37-OV SW480 cells, N-CAD and VIM were upregulated, whereas E-CAD was downregulated. *P<0.05. (C) Western blot analysis demonstrated that, in TRIM37-KD SW620 cells, the epithelial marker E-CAD was upregulated, whereas N-CAD and VIM, the mesenchymal markers, were downregulated. (D) In TRIM37-OV SW480 cells, E-CAD was downregulated, whereas N-CAD and VIM were upregulated. TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression; E-CAD, E-cadherin; N-CAD, N-cadherin; VIM, vimentin.

Journal: Molecular Medicine Reports

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

doi: 10.3892/mmr.2017.6125

Figure Lengend Snippet: TRIM37 promotes the invasion of colorectal cancer cells via EMT. (A) Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of several components that were involved in EMT. In the TRIM37-KD SW620 cells, N-CAD and VIM were downregulated, whereas E-CAD was upregulated. *P<0.05. (B) In the TRIM37-OV SW480 cells, N-CAD and VIM were upregulated, whereas E-CAD was downregulated. *P<0.05. (C) Western blot analysis demonstrated that, in TRIM37-KD SW620 cells, the epithelial marker E-CAD was upregulated, whereas N-CAD and VIM, the mesenchymal markers, were downregulated. (D) In TRIM37-OV SW480 cells, E-CAD was downregulated, whereas N-CAD and VIM were upregulated. TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression; E-CAD, E-cadherin; N-CAD, N-cadherin; VIM, vimentin.

Article Snippet: Primary antibodies for TRIM37 (1:500; cat. no. sc-49548, Santa Cruz Biotechnology, Inc.), GAPDH (1:1,000; cat. no. sc-293335, Santa Cruz Biotechnology, Inc.), E-cadherin (1:500; cat. no. ab76055, Abcam, Cambridge, UK), N-cadherin (1:500; cat. no. ab12221, Abcam) and vimentin (1;500; cat. no. ab72547, Abcam) were incubated overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Marker, Over Expression

mRNA and protein expression levels of TRIM37 are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.

Journal: Molecular Medicine Reports

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

doi: 10.3892/mmr.2017.6125

Figure Lengend Snippet: mRNA and protein expression levels of TRIM37 are increased in CRC cells relative to normal cells. (A) The mRNA expression level of TRIM37 in 30 paired CRC samples and adjacent normal tissues was detected by reverse transcription-quantitative polymerase chain reaction. The TRIM37 expression was normalized to β-actin. *P<0.001. (B) Representative immunohistochemical images of TRIM37 in matched CRC tissues (lower panel) and adjacent non-cancerous tissues (upper panel). Magnification, ×200. (C) Western blot analysis was used to examine the protein expression level of TRIM37 in CRC samples and paired adjacent normal tissues. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; T, CRC tissues; N, non-cancerous adjacent tissues.

Article Snippet: After blocking the enzymatic activity of endogenous peroxidase, slides were immunolabeled using TRIM37 primary antibody (1:200; cat. no. sc-49548, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Western Blot

TRIM37 promotes the proliferation, migration and invasion abilities in CRC cells. (A) TRIM37-KD in SW620 cells inhibited cell proliferation, whereas TRIM37-OV in SW480 cells increased proliferation. *P<0.05. (B) Crystal violet assay was used to analyze the effects of TRIM37 on the growth of CRC cells. (C) Representative images of the wound healing assay of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. (D) Representative images of the Transwell assays of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. Data are representative of three independent experiments. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression.

Journal: Molecular Medicine Reports

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

doi: 10.3892/mmr.2017.6125

Figure Lengend Snippet: TRIM37 promotes the proliferation, migration and invasion abilities in CRC cells. (A) TRIM37-KD in SW620 cells inhibited cell proliferation, whereas TRIM37-OV in SW480 cells increased proliferation. *P<0.05. (B) Crystal violet assay was used to analyze the effects of TRIM37 on the growth of CRC cells. (C) Representative images of the wound healing assay of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. (D) Representative images of the Transwell assays of TRIM37-KD and TRIM37-OV cells with control. Magnification, ×200. Data are representative of three independent experiments. CRC, colorectal cancer; TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression.

Article Snippet: After blocking the enzymatic activity of endogenous peroxidase, slides were immunolabeled using TRIM37 primary antibody (1:200; cat. no. sc-49548, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Migration, Crystal Violet Assay, Wound Healing Assay, Over Expression

TRIM37 promotes the invasion of colorectal cancer cells via EMT. (A) Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of several components that were involved in EMT. In the TRIM37-KD SW620 cells, N-CAD and VIM were downregulated, whereas E-CAD was upregulated. *P<0.05. (B) In the TRIM37-OV SW480 cells, N-CAD and VIM were upregulated, whereas E-CAD was downregulated. *P<0.05. (C) Western blot analysis demonstrated that, in TRIM37-KD SW620 cells, the epithelial marker E-CAD was upregulated, whereas N-CAD and VIM, the mesenchymal markers, were downregulated. (D) In TRIM37-OV SW480 cells, E-CAD was downregulated, whereas N-CAD and VIM were upregulated. TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression; E-CAD, E-cadherin; N-CAD, N-cadherin; VIM, vimentin.

Journal: Molecular Medicine Reports

Article Title: TRIM37 promotes epithelial-mesenchymal transition in colorectal cancer

doi: 10.3892/mmr.2017.6125

Figure Lengend Snippet: TRIM37 promotes the invasion of colorectal cancer cells via EMT. (A) Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of several components that were involved in EMT. In the TRIM37-KD SW620 cells, N-CAD and VIM were downregulated, whereas E-CAD was upregulated. *P<0.05. (B) In the TRIM37-OV SW480 cells, N-CAD and VIM were upregulated, whereas E-CAD was downregulated. *P<0.05. (C) Western blot analysis demonstrated that, in TRIM37-KD SW620 cells, the epithelial marker E-CAD was upregulated, whereas N-CAD and VIM, the mesenchymal markers, were downregulated. (D) In TRIM37-OV SW480 cells, E-CAD was downregulated, whereas N-CAD and VIM were upregulated. TRIM37, tripartite motif containing 37; TRIM 37-KD, TRIM37 knockdown; TRIM37-OV, TRIM37 overexpression; E-CAD, E-cadherin; N-CAD, N-cadherin; VIM, vimentin.

Article Snippet: After blocking the enzymatic activity of endogenous peroxidase, slides were immunolabeled using TRIM37 primary antibody (1:200; cat. no. sc-49548, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Marker, Over Expression