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Becton Dickinson primaria t25 flasks
Primaria T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primaria t25 flasks/product/Becton Dickinson
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primaria t25 flasks - by Bioz Stars, 2020-04
88/100 stars

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Modification:

Article Title: Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture
Article Snippet: .. Cell culture On sorting, the primary cell populations were plated in Primaria T25 flasks (#3813, Becton Dickenson) in the presence of ‘FAD2' ((DMEM, high glucose, no calcium, Life Technologies):Ham's F12 Nutrient Mixture (F12, Life Technologies) 3:1 v/v) with 2 mM glutamine, 0.5 μg ml−1 hydrocortisone, 5 μg ml−1 insulin, 10 ng ml−1 cholera toxin (Sigma-Aldrich), 10 ng ml−1 epidermal growth factor (Peprotech), 1.8 × 10–4 M adenine, 10 μM Y-27632 (Y0503, Sigma-Aldrich or 1683, Axon Medchem) and 5% fetal bovine serum (Sigma-Aldrich), modified from refs , ). .. On plating, which could take up to 2 days for the luminal ERneg (CD166low /CD117high ) cells and thus determined the time point for addition of TGFβR2i, a combination of the selective inhibitor of TGFβ type I receptor activin receptor-like kinase ALK5, ALK4 and ALK7, SB431542 (ref. ) (10 μM, S4317, Sigma-Aldrich or 1661, Axon Medchem) and an inhibitor of autophosphorylation of ALK5, RepSox (25 or 50 μM, R0158, Sigma-Aldrich) was added.

Concentration Assay:

Article Title: Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture
Article Snippet: Cell culture On sorting, the primary cell populations were plated in Primaria T25 flasks (#3813, Becton Dickenson) in the presence of ‘FAD2' ((DMEM, high glucose, no calcium, Life Technologies):Ham's F12 Nutrient Mixture (F12, Life Technologies) 3:1 v/v) with 2 mM glutamine, 0.5 μg ml−1 hydrocortisone, 5 μg ml−1 insulin, 10 ng ml−1 cholera toxin (Sigma-Aldrich), 10 ng ml−1 epidermal growth factor (Peprotech), 1.8 × 10–4 M adenine, 10 μM Y-27632 (Y0503, Sigma-Aldrich or 1683, Axon Medchem) and 5% fetal bovine serum (Sigma-Aldrich), modified from refs , ). .. To test the specificity of the effect of TGFβR2i, in some experiments SB431542 alone or the double concentration of SB431542 was used instead of TGFβR2i, or RepSox was replaced by another TGFβ type I receptor activin receptor-like kinase ALK5 inhibitor, SD208 (1 μM, Tocris Bioscience).

Cell Culture:

Article Title: Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture
Article Snippet: .. Cell culture On sorting, the primary cell populations were plated in Primaria T25 flasks (#3813, Becton Dickenson) in the presence of ‘FAD2' ((DMEM, high glucose, no calcium, Life Technologies):Ham's F12 Nutrient Mixture (F12, Life Technologies) 3:1 v/v) with 2 mM glutamine, 0.5 μg ml−1 hydrocortisone, 5 μg ml−1 insulin, 10 ng ml−1 cholera toxin (Sigma-Aldrich), 10 ng ml−1 epidermal growth factor (Peprotech), 1.8 × 10–4 M adenine, 10 μM Y-27632 (Y0503, Sigma-Aldrich or 1683, Axon Medchem) and 5% fetal bovine serum (Sigma-Aldrich), modified from refs , ). .. On plating, which could take up to 2 days for the luminal ERneg (CD166low /CD117high ) cells and thus determined the time point for addition of TGFβR2i, a combination of the selective inhibitor of TGFβ type I receptor activin receptor-like kinase ALK5, ALK4 and ALK7, SB431542 (ref. ) (10 μM, S4317, Sigma-Aldrich or 1661, Axon Medchem) and an inhibitor of autophosphorylation of ALK5, RepSox (25 or 50 μM, R0158, Sigma-Aldrich) was added.

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    Becton Dickinson t25 flasks
    T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Becton Dickinson
    Average 94 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    96
    Becton Dickinson primaria cell culture flasks t25
    Primaria Cell Culture Flasks T25, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primaria cell culture flasks t25/product/Becton Dickinson
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    primaria cell culture flasks t25 - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

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