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Becton Dickinson primaria 6 well plates
Primaria 6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primaria 6 well plates/product/Becton Dickinson
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primaria 6 well plates - by Bioz Stars, 2020-09
88/100 stars

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Centrifugation:

Article Title: Tacaribe Virus but Not Junin Virus Infection Induces Cytokine Release from Primary Human Monocytes and Macrophages
Article Snippet: .. After the second centrifugation, the cells were resuspended in RPMI-1640 with 2% human AB serum (Sigma) at a concentration of 1.5×106 cells/ml, and 4 ml per well was seeded into Primaria 6-well plates (BD Biosciences) and incubated at 37°C and 5% CO2 . ..

Isolation:

Article Title: Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
Article Snippet: .. Isolation of primary murine and human hepatocytes Murine hepatocytes were isolated from C57BL/6 mice using a 2-step liberase (Sigma-Aldrich) perfusion as described previously [ , ] and cultured in Primaria 6-well plates (BD Biosciences, Franklin Lakes, USA) at a density of 1 × 106 cells per well in 2 mL of HBM Basal Medium supplemented with HCM SingleQuots (Lonza, Basel, Switzerland). .. Hepatocytes were infected with CVB3 at MOIs 0.01, 0.1, and 1 in dublicates or triplicates and incubated for 24 hr at 37°C.

Article Title: Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
Article Snippet: .. Murine hepatocytes were isolated from C57BL/6 mice using a 2-step liberase (Sigma-Aldrich) perfusion as described previously [ , ] and cultured in Primaria 6-well plates (BD Biosciences, Franklin Lakes, USA) at a density of 1 × 106 cells per well in 2 mL of HBM Basal Medium supplemented with HCM SingleQuots (Lonza, Basel, Switzerland). .. Hepatocytes were infected with CVB3 at MOIs 0.01, 0.1, and 1 in dublicates or triplicates and incubated for 24 hr at 37°C.

Cell Culture:

Article Title: Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
Article Snippet: .. Isolation of primary murine and human hepatocytes Murine hepatocytes were isolated from C57BL/6 mice using a 2-step liberase (Sigma-Aldrich) perfusion as described previously [ , ] and cultured in Primaria 6-well plates (BD Biosciences, Franklin Lakes, USA) at a density of 1 × 106 cells per well in 2 mL of HBM Basal Medium supplemented with HCM SingleQuots (Lonza, Basel, Switzerland). .. Hepatocytes were infected with CVB3 at MOIs 0.01, 0.1, and 1 in dublicates or triplicates and incubated for 24 hr at 37°C.

Article Title: Slit2/Robo4 Signaling: Potential Role of a VEGF-Antagonist Pathway to Regulate Luteal Permeability
Article Snippet: .. Pooled LGCs were seeded into Primaria 6-well plates (BD Falcon), ½ × 6 cm culture dishes for each well with 2 ml DMEM Hamʼs F12 cell culture medium, 10 % FBS (PAA Laboratories) and 1 % Fungizone (Promocell, Heidelberg, Germany). .. Each well was stimulated differently, with one control (only medium) or hCG 1000 IU/ml (Predalon, Essex Pharma, Munich, Germany) or 100 ng/ml VEGFA inhibitor [VEGF Recepto-1(Flt-1) Fc Chimera, Sigma-Aldrich, St. Louis, MO, USA], or combination of both.

Article Title: Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
Article Snippet: .. Murine hepatocytes were isolated from C57BL/6 mice using a 2-step liberase (Sigma-Aldrich) perfusion as described previously [ , ] and cultured in Primaria 6-well plates (BD Biosciences, Franklin Lakes, USA) at a density of 1 × 106 cells per well in 2 mL of HBM Basal Medium supplemented with HCM SingleQuots (Lonza, Basel, Switzerland). .. Hepatocytes were infected with CVB3 at MOIs 0.01, 0.1, and 1 in dublicates or triplicates and incubated for 24 hr at 37°C.

Mouse Assay:

Article Title: Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
Article Snippet: .. Isolation of primary murine and human hepatocytes Murine hepatocytes were isolated from C57BL/6 mice using a 2-step liberase (Sigma-Aldrich) perfusion as described previously [ , ] and cultured in Primaria 6-well plates (BD Biosciences, Franklin Lakes, USA) at a density of 1 × 106 cells per well in 2 mL of HBM Basal Medium supplemented with HCM SingleQuots (Lonza, Basel, Switzerland). .. Hepatocytes were infected with CVB3 at MOIs 0.01, 0.1, and 1 in dublicates or triplicates and incubated for 24 hr at 37°C.

Article Title: Interferon-beta expression and type I interferon receptor signaling of hepatocytes prevent hepatic necrosis and virus dissemination in Coxsackievirus B3-infected mice
Article Snippet: .. Murine hepatocytes were isolated from C57BL/6 mice using a 2-step liberase (Sigma-Aldrich) perfusion as described previously [ , ] and cultured in Primaria 6-well plates (BD Biosciences, Franklin Lakes, USA) at a density of 1 × 106 cells per well in 2 mL of HBM Basal Medium supplemented with HCM SingleQuots (Lonza, Basel, Switzerland). .. Hepatocytes were infected with CVB3 at MOIs 0.01, 0.1, and 1 in dublicates or triplicates and incubated for 24 hr at 37°C.

Concentration Assay:

Article Title: Tacaribe Virus but Not Junin Virus Infection Induces Cytokine Release from Primary Human Monocytes and Macrophages
Article Snippet: .. After the second centrifugation, the cells were resuspended in RPMI-1640 with 2% human AB serum (Sigma) at a concentration of 1.5×106 cells/ml, and 4 ml per well was seeded into Primaria 6-well plates (BD Biosciences) and incubated at 37°C and 5% CO2 . ..

Incubation:

Article Title: Tacaribe Virus but Not Junin Virus Infection Induces Cytokine Release from Primary Human Monocytes and Macrophages
Article Snippet: .. After the second centrifugation, the cells were resuspended in RPMI-1640 with 2% human AB serum (Sigma) at a concentration of 1.5×106 cells/ml, and 4 ml per well was seeded into Primaria 6-well plates (BD Biosciences) and incubated at 37°C and 5% CO2 . ..

Modification:

Article Title: Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes
Article Snippet: .. HFLCs were seeded in DMEM culture medium (Dulbecco's modified Eagle's medium, BioWhittaker) containing 10% heat-inactivated fetal bovine serum (HI-FBS, BioWhittaker), 2 mM L-glutamine (BioWhittaker), 1 μM dexamethason (Sigma), 10 μg/mL insulin, 5.5 μg/mL transferrin, 6.7 ng/mL selenium-X (ITS mix, Life Technology), 100 U/mL penicillin, 100 μg/mL streptomycin (penicillin/streptomycin mix, BioWhittaker) at a density of approximately 3*105 cells/cm2 in Primaria 6-well plates (BD Falcon). ..

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    Becton Dickinson 6 well primaria culture dish
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    Becton Dickinson 6 well bd falcon primaria tissue culture plates
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    Becton Dickinson 6 well tissue culture plates
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    Becton Dickinson 12 well collagen coated culture plates
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