Structured Review

OriGene pp5c open reading frame orf construct
Pp5c Open Reading Frame Orf Construct, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pp5c open reading frame orf construct/product/OriGene
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pp5c open reading frame orf construct - by Bioz Stars, 2020-09
90/100 stars

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Related Articles

Clone Assay:

Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus
Article Snippet: .. PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF. .. Cell culture and transfection Peripheral blood mononuclear cells (PBMCs) were isolated from freshly drawn venous blood of healthy volunteers and lupus patients by Ficoll density gradient centrifugation, then cultured in RPMI 1640 supplemented with 10% fetal calf serum and stimulated with phytohemagglutinin (PHA) and IL-2 using previously published protocols [ ].

Generated:

Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus
Article Snippet: .. PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF. .. Cell culture and transfection Peripheral blood mononuclear cells (PBMCs) were isolated from freshly drawn venous blood of healthy volunteers and lupus patients by Ficoll density gradient centrifugation, then cultured in RPMI 1640 supplemented with 10% fetal calf serum and stimulated with phytohemagglutinin (PHA) and IL-2 using previously published protocols [ ].

Construct:

Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus
Article Snippet: .. PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF. .. Cell culture and transfection Peripheral blood mononuclear cells (PBMCs) were isolated from freshly drawn venous blood of healthy volunteers and lupus patients by Ficoll density gradient centrifugation, then cultured in RPMI 1640 supplemented with 10% fetal calf serum and stimulated with phytohemagglutinin (PHA) and IL-2 using previously published protocols [ ].

Plasmid Preparation:

Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus
Article Snippet: .. PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF. .. Cell culture and transfection Peripheral blood mononuclear cells (PBMCs) were isolated from freshly drawn venous blood of healthy volunteers and lupus patients by Ficoll density gradient centrifugation, then cultured in RPMI 1640 supplemented with 10% fetal calf serum and stimulated with phytohemagglutinin (PHA) and IL-2 using previously published protocols [ ].

Expressing:

Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus
Article Snippet: .. PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF. .. Cell culture and transfection Peripheral blood mononuclear cells (PBMCs) were isolated from freshly drawn venous blood of healthy volunteers and lupus patients by Ficoll density gradient centrifugation, then cultured in RPMI 1640 supplemented with 10% fetal calf serum and stimulated with phytohemagglutinin (PHA) and IL-2 using previously published protocols [ ].

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    OriGene pp5c open reading frame orf construct
    Pp5c Open Reading Frame Orf Construct, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp5c open reading frame orf construct/product/OriGene
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pp5c open reading frame orf construct - by Bioz Stars, 2020-09
    90/100 stars
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