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MJ Research pcr reactions
Specificity of Shh activity. A , <t>QC-PCR</t> gel. Lanes 1–4 are cDNA from midbrain cultures that have been coamplified with successive fourfold dilutions of mimic oligo. Lane 5 is DNA marker lane. <t>Ptc</t> target is 254 bp and mimic is 100 bp. B , Representative plot (corresponding to A ) of the log concentration of competitive mimic versus the log of the obtained band densities of target and mimic PCR substrates demonstrates the linearity of the amplification reaction. The extrapolated value of ptc message in the cDNA tested is determined to be equal to the value of mimic concentration where Log D s / D m = 0. See main text for details of the procedure. Doses in ng/ml; D s = density of test substrate; D m = density of competitive mimic. The r 2 value shows that determinations made within this range vary within 3%. C , Administration of Shh induces ptc expression in a dose– response that parallels the survival curve. The values are expressed as number of target molecules ( Log D s ) per total amount of cDNA used in each reaction as measured by optical density at 260 nm ( OD ) and were determined as demonstrated in A and B . At 4 d in vitro Shh at 5 ng/ml increases ptc expression over control, and 50 ng/ml increases expression of ptc over the level found in the ventral mesencephalon at the time of dissection. D , Affinity-purified anti-Shh antibody inhibited the Shh neurotrophic response ( p
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1) Product Images from "Sonic Hedgehog Promotes the Survival of Specific CNS Neuron Populations and Protects These Cells from Toxic Insult In Vitro"

Article Title: Sonic Hedgehog Promotes the Survival of Specific CNS Neuron Populations and Protects These Cells from Toxic Insult In Vitro

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.17-15-05891.1997

Specificity of Shh activity. A , QC-PCR gel. Lanes 1–4 are cDNA from midbrain cultures that have been coamplified with successive fourfold dilutions of mimic oligo. Lane 5 is DNA marker lane. Ptc target is 254 bp and mimic is 100 bp. B , Representative plot (corresponding to A ) of the log concentration of competitive mimic versus the log of the obtained band densities of target and mimic PCR substrates demonstrates the linearity of the amplification reaction. The extrapolated value of ptc message in the cDNA tested is determined to be equal to the value of mimic concentration where Log D s / D m = 0. See main text for details of the procedure. Doses in ng/ml; D s = density of test substrate; D m = density of competitive mimic. The r 2 value shows that determinations made within this range vary within 3%. C , Administration of Shh induces ptc expression in a dose– response that parallels the survival curve. The values are expressed as number of target molecules ( Log D s ) per total amount of cDNA used in each reaction as measured by optical density at 260 nm ( OD ) and were determined as demonstrated in A and B . At 4 d in vitro Shh at 5 ng/ml increases ptc expression over control, and 50 ng/ml increases expression of ptc over the level found in the ventral mesencephalon at the time of dissection. D , Affinity-purified anti-Shh antibody inhibited the Shh neurotrophic response ( p
Figure Legend Snippet: Specificity of Shh activity. A , QC-PCR gel. Lanes 1–4 are cDNA from midbrain cultures that have been coamplified with successive fourfold dilutions of mimic oligo. Lane 5 is DNA marker lane. Ptc target is 254 bp and mimic is 100 bp. B , Representative plot (corresponding to A ) of the log concentration of competitive mimic versus the log of the obtained band densities of target and mimic PCR substrates demonstrates the linearity of the amplification reaction. The extrapolated value of ptc message in the cDNA tested is determined to be equal to the value of mimic concentration where Log D s / D m = 0. See main text for details of the procedure. Doses in ng/ml; D s = density of test substrate; D m = density of competitive mimic. The r 2 value shows that determinations made within this range vary within 3%. C , Administration of Shh induces ptc expression in a dose– response that parallels the survival curve. The values are expressed as number of target molecules ( Log D s ) per total amount of cDNA used in each reaction as measured by optical density at 260 nm ( OD ) and were determined as demonstrated in A and B . At 4 d in vitro Shh at 5 ng/ml increases ptc expression over control, and 50 ng/ml increases expression of ptc over the level found in the ventral mesencephalon at the time of dissection. D , Affinity-purified anti-Shh antibody inhibited the Shh neurotrophic response ( p

Techniques Used: Activity Assay, Polymerase Chain Reaction, Marker, Concentration Assay, Amplification, Expressing, In Vitro, Dissection, Affinity Purification

2) Product Images from "Physiological Ecology of Stenoxybacter acetivorans, an Obligate Microaerophile in Termite Guts ▿"

Article Title: Physiological Ecology of Stenoxybacter acetivorans, an Obligate Microaerophile in Termite Guts ▿

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00787-07

Gelstar-stained agarose gel electropherograms of RT-PCR products for (A) acetate kinase ( ack ), (B) phosphotransacetylase ( pta ), and (C) the O 2 -binding subunit of cbb 3 terminal oxidase ( ccoN ). The template for RT-PCR was RNA purified from R. flavipes gut homogenates, and amplification was done with S. acetivorans -specific, gene-targeted primers. Lane 1, 1-kb DNA ladder (the bands represent, from the bottom to the top, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0, and 10.0 kb); lane 2, RT-PCR product obtained using R. flavipes gut homogenate RNA as the template; lane 3, same as lane 2, but without addition of RT; lane 4, RT-PCR with S. acetivorans TAM-DN1 genomic DNA as the template; lane 5, RT-PCR without a template. The arrows indicate the correct size of the anticipated RT-PCR product.
Figure Legend Snippet: Gelstar-stained agarose gel electropherograms of RT-PCR products for (A) acetate kinase ( ack ), (B) phosphotransacetylase ( pta ), and (C) the O 2 -binding subunit of cbb 3 terminal oxidase ( ccoN ). The template for RT-PCR was RNA purified from R. flavipes gut homogenates, and amplification was done with S. acetivorans -specific, gene-targeted primers. Lane 1, 1-kb DNA ladder (the bands represent, from the bottom to the top, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0, and 10.0 kb); lane 2, RT-PCR product obtained using R. flavipes gut homogenate RNA as the template; lane 3, same as lane 2, but without addition of RT; lane 4, RT-PCR with S. acetivorans TAM-DN1 genomic DNA as the template; lane 5, RT-PCR without a template. The arrows indicate the correct size of the anticipated RT-PCR product.

Techniques Used: Staining, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Purification, Amplification

3) Product Images from "Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5′-Terminal Genomic Deletions"

Article Title: Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5′-Terminal Genomic Deletions

Journal: Journal of Virology

doi: 10.1128/JVI.01308-15

Loss of a functional CRE(2C) leads to evolution of 5′-terminal deletions in CVB3-CKO after 8 days of replication in HeLa cells. (A) Schematic of how amplification with Pin primers works. (B and C) Relative position in the CVB3 genome of tagged primers used to amplify the 5′-terminal sequence (B) or to amplify the sequence in a region of the 5′ NTR where deletions do not occur (C). (D) Agarose gel analysis of E3-primed cDNA amplified with primers Pin5End1 and E3 or primers Pin5End5 and E3 and then subsequently amplified with primers Pin and SReturn (lanes 2 and 3, CVB3-CKO purified virus preparation; lanes 5 and 6, CVB3-CKO T7 transcripts; lanes 8 and 9, wt CVB3 preparation; lanes 11 and 12, wt CVB3 T7 transcripts). To detect virus using a region of the 5′ NTR where deletions do not occur, cDNA was made with primer PinE3 and amplified with primers S5 and Pin (lane 4, CVB3-CKO preparation; lane 7, CVB3-CKO T7 transcripts; lane 10, wt CVB3 preparations; lane 13, wt CVB3 T7 transcripts). The cDNA of 400 (Pin5End1) or 1,400 (Pin5End5) viral RNA or T7-transcribed RNA (controls) copies was used in each PCR. Arrows, the loss of sequence from the 5′ terminus of CVB3-CKO (lanes 2 and 3) compared to the T7 or wt virus controls (lanes 5 and 6, 8 and 9, and 11 and 12). However, CVB3-CKO could be detected by RT-PCR priming in a region of the 5′ NTR where deletions do not occur (compare lane 4 to lanes 7, 10, and 13). (E) Controls (lanes 2 to 4, no-cDNA PCR controls; lanes 5 to 7, no-template RT-PCR controls; lanes 8 to 10, tissue culture controls) were negative. Lanes 1 and 4 in panel D and lanes 1 and 11 in panel E contain molecular size markers.
Figure Legend Snippet: Loss of a functional CRE(2C) leads to evolution of 5′-terminal deletions in CVB3-CKO after 8 days of replication in HeLa cells. (A) Schematic of how amplification with Pin primers works. (B and C) Relative position in the CVB3 genome of tagged primers used to amplify the 5′-terminal sequence (B) or to amplify the sequence in a region of the 5′ NTR where deletions do not occur (C). (D) Agarose gel analysis of E3-primed cDNA amplified with primers Pin5End1 and E3 or primers Pin5End5 and E3 and then subsequently amplified with primers Pin and SReturn (lanes 2 and 3, CVB3-CKO purified virus preparation; lanes 5 and 6, CVB3-CKO T7 transcripts; lanes 8 and 9, wt CVB3 preparation; lanes 11 and 12, wt CVB3 T7 transcripts). To detect virus using a region of the 5′ NTR where deletions do not occur, cDNA was made with primer PinE3 and amplified with primers S5 and Pin (lane 4, CVB3-CKO preparation; lane 7, CVB3-CKO T7 transcripts; lane 10, wt CVB3 preparations; lane 13, wt CVB3 T7 transcripts). The cDNA of 400 (Pin5End1) or 1,400 (Pin5End5) viral RNA or T7-transcribed RNA (controls) copies was used in each PCR. Arrows, the loss of sequence from the 5′ terminus of CVB3-CKO (lanes 2 and 3) compared to the T7 or wt virus controls (lanes 5 and 6, 8 and 9, and 11 and 12). However, CVB3-CKO could be detected by RT-PCR priming in a region of the 5′ NTR where deletions do not occur (compare lane 4 to lanes 7, 10, and 13). (E) Controls (lanes 2 to 4, no-cDNA PCR controls; lanes 5 to 7, no-template RT-PCR controls; lanes 8 to 10, tissue culture controls) were negative. Lanes 1 and 4 in panel D and lanes 1 and 11 in panel E contain molecular size markers.

Techniques Used: Functional Assay, Amplification, Sequencing, Agarose Gel Electrophoresis, Purification, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

CVB3-CKO replicates without observable CPE and can be passaged from purified HeLa cell lysates and neutralized by anti-CVB3 neutralizing antibody. (A to F) HeLa cell monolayers at 5 days postinoculation with wt CVB3 (C, F) or CVB3-CKO (B, E) with anti-CVB3 neutralizing antibody (+ Ab) (A to C) or without antibody (− Ab) (D to E). Monolayers without virus inoculation (A, D) were also cultured. CVB3-CKO does not produce CPE after infection. (B) Neutralizing antibody prevents CPE with wt CVB3 (C) at 5 days postinoculation, whereas CPE occurs without neutralizing antibody (F). Magnifications, ×100. (G) Nested primers used to amplify the sequence in the 5′ NTR of CVB3 to detect viral RNA after inoculation of cell cultures. Primers are specific for a region of the 5′ NTR where deletions do not occur. (H) Total RNA was extracted from HeLa cell monolayers at 5 days postinoculation and assayed for the presence of viral RNA by nested PCR and gel electrophoresis. wt CVB3 was detected (lanes 8 and 9), as was noncytopathic CVB3-CKO (arrows, lanes 4 and 5). The absence of bands when neutralizing antibody was present in the cell cultures (lanes 2 and 3, CVB3-CKO; lanes 6 and 7, wt CVB3) demonstrates that the antibody effectively suppressed productive infection. Lane 1, molecular size markers. (I) All controls (lanes 2 to 4, no-cDNA PCR controls; lanes 5 to 7, no-RNA RT-PCR controls; lanes 8 to 10, tissue culture controls) were negative. Lanes 1 and 10, molecular size markers. (J) Additional virus preparations were tested to verify the observations made from panel H. CVB3-CKO can be passaged in cell culture (lane 3), whereas the T7 RNA transcript control cannot (lane 1), and CVB3-CKO can be neutralized with anti-CVB3 neutralizing antibody (lane 2). Lane 4, molecular size markers.
Figure Legend Snippet: CVB3-CKO replicates without observable CPE and can be passaged from purified HeLa cell lysates and neutralized by anti-CVB3 neutralizing antibody. (A to F) HeLa cell monolayers at 5 days postinoculation with wt CVB3 (C, F) or CVB3-CKO (B, E) with anti-CVB3 neutralizing antibody (+ Ab) (A to C) or without antibody (− Ab) (D to E). Monolayers without virus inoculation (A, D) were also cultured. CVB3-CKO does not produce CPE after infection. (B) Neutralizing antibody prevents CPE with wt CVB3 (C) at 5 days postinoculation, whereas CPE occurs without neutralizing antibody (F). Magnifications, ×100. (G) Nested primers used to amplify the sequence in the 5′ NTR of CVB3 to detect viral RNA after inoculation of cell cultures. Primers are specific for a region of the 5′ NTR where deletions do not occur. (H) Total RNA was extracted from HeLa cell monolayers at 5 days postinoculation and assayed for the presence of viral RNA by nested PCR and gel electrophoresis. wt CVB3 was detected (lanes 8 and 9), as was noncytopathic CVB3-CKO (arrows, lanes 4 and 5). The absence of bands when neutralizing antibody was present in the cell cultures (lanes 2 and 3, CVB3-CKO; lanes 6 and 7, wt CVB3) demonstrates that the antibody effectively suppressed productive infection. Lane 1, molecular size markers. (I) All controls (lanes 2 to 4, no-cDNA PCR controls; lanes 5 to 7, no-RNA RT-PCR controls; lanes 8 to 10, tissue culture controls) were negative. Lanes 1 and 10, molecular size markers. (J) Additional virus preparations were tested to verify the observations made from panel H. CVB3-CKO can be passaged in cell culture (lane 3), whereas the T7 RNA transcript control cannot (lane 1), and CVB3-CKO can be neutralized with anti-CVB3 neutralizing antibody (lane 2). Lane 4, molecular size markers.

Techniques Used: Purification, Cell Culture, Infection, Sequencing, Nested PCR, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

T7 RNA transcripts from CVB3 and CVB3-CKO clones can be transfected into mice, and virus replicates posttransfection. Nested RT-PCR could detect viral RNA using total RNA from the hearts and spleens of mice transfected with wt CVB3 or CVB3-CKO T7 RNA transcripts. (A) A representative gel demonstrating the necessary 10-fold serial dilutions of total extracted tissue RNA (in this case, from heart) to detect the low level of replication of CVB3-CKO in mouse tissue at 20 days posttransfection (lanes 1 and 8, molecular size marker; lane 2, no-RNA RT-PCR control; lane 3, untransfected mouse RNA; lane 4, 10-fold dilution of RNA from a CVB3-CKO-transfected mouse; lane 5, 100-fold dilution; lane 6, 1,000-fold dilution; lane 7, 10,000-fold dilution). CVB3-CKO RNA was detectable only at a 1,000-fold dilution of RNA (arrow, lane 6). (B) Detection of viral RNA in the spleens of transfected mice (lanes 1 and 9, molecular size marker; lane 2, no-cDNA PCR control; lane 3, no-RNA RT-PCR control; lane 4, HeLa cell RNA control; lane 5, 1,000 copies of CVB3 cDNA from T7 transcripts; lane 6, untransfected mouse RNA; lane 7, 1,000-fold dilution of RNA obtained from a CVB3-CKO-transfected mouse at day 20 posttransfection; lane 8, 100,000-fold dilution of RNA obtained from a wt CVB3-transfected mouse at day 8 posttransfection). (C) Detection of viral RNA in the hearts of transfected mice (lanes 1 and 8, molecular size marker; lane 2, no-cDNA PCR control; lane 3, no-RNA RT-PCR control; lane 4, untransfected mouse RNA; lane 5, 1,000 copies of CVB3 cDNA from T7 transcripts; lane 6, 100-fold dilution of RNA obtained from a CVB3-CKO-transfected mouse at day 20 posttransfection; lane 7, 10,000-fold dilution of RNA obtained from a wt CVB3-transfected mouse at day 4 posttransfection). (D to K) RNase-treated homogenates of spleens were passaged onto HeLa cells at approximately 90% confluence and incubated overnight with (D to G) or without (H to K) neutralizing antibodies (D and H, no homogenate; E and I, control mouse homogenate; F and J, homogenate obtained from CVB3-CKO-transfected mice at day 20 posttransfection; G and K, homogenate obtained from CVB3-transfected mice at day 8 posttransfection). Magnifications, ×100. CPE was observed only in cultures inoculated with homogenates from mice transfected with wt CVB3 RNA (K). (L) Following 5 days of incubation, total RNA was tested by nested RT-PCR and analyzed on 2% agarose gels (lanes 1 and 14, molecular size marker; lanes 2 and 3, CVB3-CKO plus neutralizing antibody; lanes 4 and 5, CVB3-CKO minus neutralizing antibody; lanes 6 and 7, wt CVB3 with neutralizing antibody; lanes 8 and 9, wt CVB3 without neutralizing antibody; lane 10, no-cDNA control; lane 11, no-RNA RT control; lane 12, uninfected control with neutralizing antibody; lane 13, uninfected control without neutralizing antibody), demonstrating that CVB3-CKO (arrows, lanes 4 and 5) and wt CVB3 (arrows, lanes 8 and 9) were detected in tissue homogenates following transfection of mice. The absence of amplimers in lanes 2 and 3 (CVB3-CKO) and lanes 6 and 7 (wt CVB3) demonstrates that virus is neutralized by anti-CVB3 neutralizing antibody.
Figure Legend Snippet: T7 RNA transcripts from CVB3 and CVB3-CKO clones can be transfected into mice, and virus replicates posttransfection. Nested RT-PCR could detect viral RNA using total RNA from the hearts and spleens of mice transfected with wt CVB3 or CVB3-CKO T7 RNA transcripts. (A) A representative gel demonstrating the necessary 10-fold serial dilutions of total extracted tissue RNA (in this case, from heart) to detect the low level of replication of CVB3-CKO in mouse tissue at 20 days posttransfection (lanes 1 and 8, molecular size marker; lane 2, no-RNA RT-PCR control; lane 3, untransfected mouse RNA; lane 4, 10-fold dilution of RNA from a CVB3-CKO-transfected mouse; lane 5, 100-fold dilution; lane 6, 1,000-fold dilution; lane 7, 10,000-fold dilution). CVB3-CKO RNA was detectable only at a 1,000-fold dilution of RNA (arrow, lane 6). (B) Detection of viral RNA in the spleens of transfected mice (lanes 1 and 9, molecular size marker; lane 2, no-cDNA PCR control; lane 3, no-RNA RT-PCR control; lane 4, HeLa cell RNA control; lane 5, 1,000 copies of CVB3 cDNA from T7 transcripts; lane 6, untransfected mouse RNA; lane 7, 1,000-fold dilution of RNA obtained from a CVB3-CKO-transfected mouse at day 20 posttransfection; lane 8, 100,000-fold dilution of RNA obtained from a wt CVB3-transfected mouse at day 8 posttransfection). (C) Detection of viral RNA in the hearts of transfected mice (lanes 1 and 8, molecular size marker; lane 2, no-cDNA PCR control; lane 3, no-RNA RT-PCR control; lane 4, untransfected mouse RNA; lane 5, 1,000 copies of CVB3 cDNA from T7 transcripts; lane 6, 100-fold dilution of RNA obtained from a CVB3-CKO-transfected mouse at day 20 posttransfection; lane 7, 10,000-fold dilution of RNA obtained from a wt CVB3-transfected mouse at day 4 posttransfection). (D to K) RNase-treated homogenates of spleens were passaged onto HeLa cells at approximately 90% confluence and incubated overnight with (D to G) or without (H to K) neutralizing antibodies (D and H, no homogenate; E and I, control mouse homogenate; F and J, homogenate obtained from CVB3-CKO-transfected mice at day 20 posttransfection; G and K, homogenate obtained from CVB3-transfected mice at day 8 posttransfection). Magnifications, ×100. CPE was observed only in cultures inoculated with homogenates from mice transfected with wt CVB3 RNA (K). (L) Following 5 days of incubation, total RNA was tested by nested RT-PCR and analyzed on 2% agarose gels (lanes 1 and 14, molecular size marker; lanes 2 and 3, CVB3-CKO plus neutralizing antibody; lanes 4 and 5, CVB3-CKO minus neutralizing antibody; lanes 6 and 7, wt CVB3 with neutralizing antibody; lanes 8 and 9, wt CVB3 without neutralizing antibody; lane 10, no-cDNA control; lane 11, no-RNA RT control; lane 12, uninfected control with neutralizing antibody; lane 13, uninfected control without neutralizing antibody), demonstrating that CVB3-CKO (arrows, lanes 4 and 5) and wt CVB3 (arrows, lanes 8 and 9) were detected in tissue homogenates following transfection of mice. The absence of amplimers in lanes 2 and 3 (CVB3-CKO) and lanes 6 and 7 (wt CVB3) demonstrates that virus is neutralized by anti-CVB3 neutralizing antibody.

Techniques Used: Clone Assay, Transfection, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Marker, Polymerase Chain Reaction, Incubation

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Polymerase Chain Reaction:

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Article Snippet: .. PCR was carried out in a DNA thermal cycler (MJ Research Inc., Watertown, MA), with 30 cycles of denaturation (94°C, 30 s), annealing (45 to 55°C, 1 min), and extension (72°C, 1 min 30 s) followed by a final extension step (72°C, 7 min). ..

Article Title: Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification
Article Snippet: .. The PCR was carried out in a DNA thermal cycler (MJ Research, Inc., Watertown, Mass.) with 35 cycles of denaturation (94°C, 30 s), annealing (52°C, 1 min), and extension (72°C, 1 min), followed by a final extension step (72°C, 7 min). .. The PCR amplification products were analyzed by agarose gel electrophoresis in 1.5% agarose (FMC BioProducts, Rockland, Maine) and stained with ethidium bromide.

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Article Title: Streptomyces albus Isolated from a Human Actinomycetoma and Characterized by Molecular Techniques
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Reverse Transcription Polymerase Chain Reaction:

Article Title: Cytokine Gene Expression in Innately Susceptible BALB/c Mice and Relatively Resistant C57BL/6 Mice during Infection with Virulent Burkholderia pseudomallei
Article Snippet: .. Heating cycles for RT-PCR were performed in an automated DNA thermal cycler (Bresatec; MJ Research Inc.; PTC-100). .. The resultant cDNA was stored at −20°C until PCR amplification.

Incubation:

Article Title: Streptomyces albus Isolated from a Human Actinomycetoma and Characterized by Molecular Techniques
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Amplification:

Article Title: Characterization of novel breast carcinoma-associated BA46-derived peptides in HLA-A2.1/Db-?2mtransgenic mice
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Article Title: Neuroprotection associated with alternative splicing of NMDA receptors in rat cortical neurons
Article Snippet: .. PCR amplification of cDNA calibration fragments specific for NR subunits NR1, NR2A and NR2B, respectively, was performed on a PTC-200 Thermocycler (MJ Research) in a final volume of 25 μ l PCR buffer (10 m M Tris-HCl, pH 8.3, 50 m M KCl) containing 1 μ l cDNA, 1 U Ampli-Taq DNA Polymerase (Applied Biosystems, Foster City, CA, U.S.A.), 200 n M (anti)sense primer, 200 μ M of each dNTP and 1 m M MgCl2 . .. The amplification conditions were as follows: 94°C for 5 min followed by 35 cycles of denaturation (94°C for 30 s), annealing (NR1: 58°C, NR2A: 56°C, NR2B: 56.3°C for 30 s, each), extension (72°C for 90 s) and a final extension step at 72°C for 7 min. Amplified products were separated by agarose gel electrophoresis (1.5%) and visualized by ethidium bromide fluorescence. cDNA was extracted from agarose gel by means of QIAquick Gel Extraction Kit (Qiagen) and subsequently used for the generation of cDNA reference curves for the respective NR subunits.

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Size-exclusion Chromatography:

Article Title: Functionally Expression of Metalloproteinase in Taenia solium Metacestode and Its Evaluation for Serodiagnosis of Cysticercosis
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    MJ Research polymerase chain reaction mixture
    Polymerase Chain Reaction Mixture, supplied by MJ Research, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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