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Stratagene polymerase chain reaction pcr mixtures
Polymerase Chain Reaction Pcr Mixtures, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction pcr mixtures/product/Stratagene
Average 88 stars, based on 7 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction pcr mixtures - by Bioz Stars, 2020-09
88/100 stars

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Polymerase Chain Reaction:

Article Title: Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (25 μL) contained 10 ng of template, 1× Easy A reaction buffer (Stratagene), 200 mM of each dNTP (Stratagene), 200 nM of each primer, and 1.25 U of Easy A cloning enzyme (Stratagene). .. The cycling conditions for the V1–V3 amplicon consisted of an initial denaturation of 95°C for 2 min, followed by 30 cycles of denaturation at 95°C for 40 sec, annealing at 56°C for 30 sec, extension at 72°C for 1 min and a final extension at 72°C for 7 min.

Article Title: Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (20 μ L in total) were prepared to contain 10 μ L of SYBR Green PCR mix (Stratagene, La Jolla, CA, USA), 0.3 μ L of reference dye, 1 μ L of each primer (10 μ mol/L), 1 μ L of cDNA template, and 6.7 μ L of diethylpyro-carbonate-treated water. .. Quantitative PCR analysis was carried out using the DNA Engine Mx3000P® (Agilent, Santa Clara, CA, USA) fluorescence detection system against a double-stranded DNA-specific fluorescent dye (Stratagene, La Jolla, CA, USA) according to optimized PCR protocols.

Article Title: Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (25μl) contained 10 ng of template, 1x Easy A reaction buffer (Stratagene), 200 mM of each dNTP (Stratagene), 200 nM of each primer, and 1.25 U Easy A cloning enzyme (Stratagene). .. The cycling conditions for the V3–V5 consisted of an initial denaturation of 95°C for 2 min, followed by 25 cycles of denaturation at 95°C for 40 sec, annealing at 50°C for 30 sec, extension at 72°C for 5 min, and a final extension at 72°C for 7 min. Amplicons were confirmed on 1.2% Flash Gels (Lonza) and purified with AMPure XP DNA purification beads (Beckman Coulter) according to the manufacturer's instructions and eluted in 25 μL of 1× low TE buffer (pH 8.0).

Article Title: Non-Invasive Mapping of the Gastrointestinal Microbiota Identifies Children with Inflammatory Bowel Disease
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (25 µl) contained 10 ng of template, 1× Easy A reaction buffer (Stratagene, La Jolla, CA), 200 mM of each dNTP (Stratagene), 200 nM of each primer, and 1.25 U Easy A cloning enzyme (Stratagene). .. The cycling conditions for the V3–V5 consisted of an initial denaturation of 95°C for 2 min, followed by 25 cycles of denaturation at 95°C for 40 sec, annealing at 50°C for 30 sec, extension at 72°C for 5 min and a final extension at 72°C for 7 min. Amplicons were confirmed on 1.2% Flash Gels (Lonza, Rockland, ME) and purified with AMPure XP DNA purification beads (Beckman Coulter, Danvers, MA) according to the manufacturer and eluted in 25 µL of 1× low TE buffer (pH 8.0).

Clone Assay:

Article Title: Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (25 μL) contained 10 ng of template, 1× Easy A reaction buffer (Stratagene), 200 mM of each dNTP (Stratagene), 200 nM of each primer, and 1.25 U of Easy A cloning enzyme (Stratagene). .. The cycling conditions for the V1–V3 amplicon consisted of an initial denaturation of 95°C for 2 min, followed by 30 cycles of denaturation at 95°C for 40 sec, annealing at 56°C for 30 sec, extension at 72°C for 1 min and a final extension at 72°C for 7 min.

Article Title: Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (25μl) contained 10 ng of template, 1x Easy A reaction buffer (Stratagene), 200 mM of each dNTP (Stratagene), 200 nM of each primer, and 1.25 U Easy A cloning enzyme (Stratagene). .. The cycling conditions for the V3–V5 consisted of an initial denaturation of 95°C for 2 min, followed by 25 cycles of denaturation at 95°C for 40 sec, annealing at 50°C for 30 sec, extension at 72°C for 5 min, and a final extension at 72°C for 7 min. Amplicons were confirmed on 1.2% Flash Gels (Lonza) and purified with AMPure XP DNA purification beads (Beckman Coulter) according to the manufacturer's instructions and eluted in 25 μL of 1× low TE buffer (pH 8.0).

Article Title: Non-Invasive Mapping of the Gastrointestinal Microbiota Identifies Children with Inflammatory Bowel Disease
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (25 µl) contained 10 ng of template, 1× Easy A reaction buffer (Stratagene, La Jolla, CA), 200 mM of each dNTP (Stratagene), 200 nM of each primer, and 1.25 U Easy A cloning enzyme (Stratagene). .. The cycling conditions for the V3–V5 consisted of an initial denaturation of 95°C for 2 min, followed by 25 cycles of denaturation at 95°C for 40 sec, annealing at 50°C for 30 sec, extension at 72°C for 5 min and a final extension at 72°C for 7 min. Amplicons were confirmed on 1.2% Flash Gels (Lonza, Rockland, ME) and purified with AMPure XP DNA purification beads (Beckman Coulter, Danvers, MA) according to the manufacturer and eluted in 25 µL of 1× low TE buffer (pH 8.0).

SYBR Green Assay:

Article Title: Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli
Article Snippet: .. Polymerase chain reaction (PCR) mixtures (20 μ L in total) were prepared to contain 10 μ L of SYBR Green PCR mix (Stratagene, La Jolla, CA, USA), 0.3 μ L of reference dye, 1 μ L of each primer (10 μ mol/L), 1 μ L of cDNA template, and 6.7 μ L of diethylpyro-carbonate-treated water. .. Quantitative PCR analysis was carried out using the DNA Engine Mx3000P® (Agilent, Santa Clara, CA, USA) fluorescence detection system against a double-stranded DNA-specific fluorescent dye (Stratagene, La Jolla, CA, USA) according to optimized PCR protocols.

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  • 88
    Stratagene polymerase chain reaction pcr mixtures
    Polymerase Chain Reaction Pcr Mixtures, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr mixtures/product/Stratagene
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr mixtures - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    90
    Stratagene polymerase chain reaction pcr mixture
    Polymerase Chain Reaction Pcr Mixture, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr mixture/product/Stratagene
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr mixture - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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