polymerase chain reaction pcr amplification  (Bio-Rad)

 
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    Structured Review

    Bio-Rad polymerase chain reaction pcr amplification
    Amplification of dsDNA with/without B-dUTP labeling in BST–DSN reaction. ( A ) A <t>PCR</t> product, <t>p53</t> exon 8, was used as input in BST–DSN reaction using native nucleotides (dNTPs), or alternatively, dNTPs plus biotinylated dUTP. After amplification, BST–DSN products were purified and the product size was analyzed via electrophoresis on an Agilent Bioanalyzer for native dNTPs ( B ) or for dNTPs plus B-dUTP ( C ). Similar fragment sizes were obtained in the two cases. Under the conditions applied, most BST–DSN products were between 20 and 80 bp while the range of products was ∼15–150 bp.
    Polymerase Chain Reaction Pcr Amplification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment"

    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkz870

    Amplification of dsDNA with/without B-dUTP labeling in BST–DSN reaction. ( A ) A PCR product, p53 exon 8, was used as input in BST–DSN reaction using native nucleotides (dNTPs), or alternatively, dNTPs plus biotinylated dUTP. After amplification, BST–DSN products were purified and the product size was analyzed via electrophoresis on an Agilent Bioanalyzer for native dNTPs ( B ) or for dNTPs plus B-dUTP ( C ). Similar fragment sizes were obtained in the two cases. Under the conditions applied, most BST–DSN products were between 20 and 80 bp while the range of products was ∼15–150 bp.
    Figure Legend Snippet: Amplification of dsDNA with/without B-dUTP labeling in BST–DSN reaction. ( A ) A PCR product, p53 exon 8, was used as input in BST–DSN reaction using native nucleotides (dNTPs), or alternatively, dNTPs plus biotinylated dUTP. After amplification, BST–DSN products were purified and the product size was analyzed via electrophoresis on an Agilent Bioanalyzer for native dNTPs ( B ) or for dNTPs plus B-dUTP ( C ). Similar fragment sizes were obtained in the two cases. Under the conditions applied, most BST–DSN products were between 20 and 80 bp while the range of products was ∼15–150 bp.

    Techniques Used: Amplification, Labeling, Polymerase Chain Reaction, Purification, Electrophoresis

    Related Articles

    Amplification:

    Article Title: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
    Article Snippet: .. The polymerase chain reaction (PCR) amplification was carried out using the Step-one System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative real-time PCR (qRT-PCR) results were carried out in duplicate and normalized to GAPDH.

    Article Title: Studying Early Lethality of 45,XO (Turner's Syndrome) Embryos Using Human Embryonic Stem Cells
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of individual markers (fluorescence-dye-labeled forward primer and unlabeled reverse primer) was performed in a PTC 225 DNA Engine (Bio-Rad, Hercules, CA) using 25–30 ng of genomic DNA, 6 pmoles of each primer, 1.5 mM MgCl2, 0.14 mM deoxynucleoside-5-triphosphate, 1× PCR Gold Buffer and 0.4 units of AmpliTaq Gold DNA Polymerase (both from Applied Biosystems) in a total volume of 10 µl. ..

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
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    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
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    Article Title: Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells
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    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
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    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. PCR was carried out according to Schuelke [ ] in a two-step process as follows: the first step consisted of an initial denaturing step of 94°C for 5 min, followed by 30 cycles of 94°C for 30 s, annealing temperature ( ) for 45 s, and 72°C for 45 s. The second step consisted of 8 cycles at 94°C for 30 s, 53°C for 45 s and 72°C for 45 s, and a final extension at 72°C for 10 min. Quality of PCR products was checked by electrophoresis in agarose gels (1.5% (w/v)) stained with GelRed (Biotium) under ultraviolet light.

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
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    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
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    Article Title: Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, et al. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
    Article Snippet: .. Polymerase chain reaction (PCR) amplification was performed in a thermocycler (iCycler, Bio‐Rad, Hercules, CA, USA) with initial denaturation at 95°C for 5 min, 33 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min and a final extension step of 7 min at 72°C. .. Fragment sizes of amplified DNA were determined automatically by GeneScan software V3.7.

    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of gene targets PCR reactions targeting p53, NOP14, MTMR4, ZPLD1, CDHR3, GMPR, CACNA1I, OR2S2, AGHGEF12, CACNA1C, SAMDA4, KRAS, BRAF and NGLY1 were performed on CFX ConnectTm real-time PCR machine (Bio-Rad Laboratories) using Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific) per the protocol provided in . .. All primers were synthesized by IDT (Integrated DNA Technologies, IDT) using primers depicted in .

    Synthesized:

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    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
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    Article Title: Porous nanofibrous PLLA scaffolds for vascular tissue engineering
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    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
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    Lambda DNA Preparation:

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    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Quantitative RT-PCR:

    Article Title: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
    Article Snippet: The polymerase chain reaction (PCR) amplification was carried out using the Step-one System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative real-time PCR (qRT-PCR) results were carried out in duplicate and normalized to GAPDH.

    Article Title: Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells
    Article Snippet: The polymerase chain reaction (PCR) amplification was carried out using the IQ5 System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative RT-PCR tests were carried out in duplicate and normalized to β-actin.

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
    Article Snippet: Total RNA Extraction and RT-PCR Analysis Total RNA was extracted from liver tissue samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized via a reverse transcriptase (RT) reaction, and polymerase chain reaction (PCR) amplification was performed using a thermal cycler (Bio-Rad, Hercules, CA, USA). .. Real-time RT-PCR analysis was carried out with a Bio-Rad CFX96TM (Bio-Rad) using iTaq™ SYBR Green SuperMix (Bio-Rad).

    SYBR Green Assay:

    Article Title: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
    Article Snippet: .. The polymerase chain reaction (PCR) amplification was carried out using the Step-one System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative real-time PCR (qRT-PCR) results were carried out in duplicate and normalized to GAPDH.

    Article Title: Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells
    Article Snippet: .. The polymerase chain reaction (PCR) amplification was carried out using the IQ5 System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative RT-PCR tests were carried out in duplicate and normalized to β-actin.

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
    Article Snippet: Total RNA Extraction and RT-PCR Analysis Total RNA was extracted from liver tissue samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized via a reverse transcriptase (RT) reaction, and polymerase chain reaction (PCR) amplification was performed using a thermal cycler (Bio-Rad, Hercules, CA, USA). .. Real-time RT-PCR analysis was carried out with a Bio-Rad CFX96TM (Bio-Rad) using iTaq™ SYBR Green SuperMix (Bio-Rad).

    Article Title: Porous nanofibrous PLLA scaffolds for vascular tissue engineering
    Article Snippet: .. Polymerase chain reaction (PCR) amplification was performed with primers for smooth muscle myosin heavy chain (SMMHC), Smoothelin and Myocardin (MyoCD) using SYBR Green supermix kit (Bio-rad, Hercules, CA) following the instructions. .. PCR primers and reaction conditions are described in .

    Expressing:

    Article Title: Porous nanofibrous PLLA scaffolds for vascular tissue engineering
    Article Snippet: Paragraph title: Gene expression analysis ... Polymerase chain reaction (PCR) amplification was performed with primers for smooth muscle myosin heavy chain (SMMHC), Smoothelin and Myocardin (MyoCD) using SYBR Green supermix kit (Bio-rad, Hercules, CA) following the instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
    Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction ... The polymerase chain reaction (PCR) amplification was carried out using the Step-one System (Bio-Rad) with SYBR Green Mastermix (Takara).

    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of gene targets PCR reactions targeting p53, NOP14, MTMR4, ZPLD1, CDHR3, GMPR, CACNA1I, OR2S2, AGHGEF12, CACNA1C, SAMDA4, KRAS, BRAF and NGLY1 were performed on CFX ConnectTm real-time PCR machine (Bio-Rad Laboratories) using Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific) per the protocol provided in . .. All primers were synthesized by IDT (Integrated DNA Technologies, IDT) using primers depicted in .

    Cell Culture:

    Article Title: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
    Article Snippet: Quantitative real-time polymerase chain reaction Cultured cells were lysed by TRIzol (Invitrogen, USA), and RNA was extracted according to the manufacturer’s instruction. .. The polymerase chain reaction (PCR) amplification was carried out using the Step-one System (Bio-Rad) with SYBR Green Mastermix (Takara).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
    Article Snippet: Paragraph title: Reverse Transcription–Polymerase Chain Reaction Analysis of Liver and Lung CSE mRNA ... The cDNA was used as a template for polymerase chain reaction (PCR) amplification by iQ Supermix (Bio-Rad).

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
    Article Snippet: .. Total RNA Extraction and RT-PCR Analysis Total RNA was extracted from liver tissue samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized via a reverse transcriptase (RT) reaction, and polymerase chain reaction (PCR) amplification was performed using a thermal cycler (Bio-Rad, Hercules, CA, USA). .. Real-time RT-PCR analysis was carried out with a Bio-Rad CFX96TM (Bio-Rad) using iTaq™ SYBR Green SuperMix (Bio-Rad).

    Sequencing:

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: Three fluorescent dyes (NED, FAM and HEX) were attached to the 5' end of the M13 universal primer sequence ( 5'- CACGACGTTGTAAAACGAC-3' ) following Schuelke [ ]. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
    Article Snippet: For the synthesis of the second strand we have designed the oligonucleotide primers ( ) using the sequence information of the Canis familiaris chromosome 17 whole genome shotgun sequence (GenBank). .. Briefly, polymerase chain reaction (PCR) amplification was carried out in a thermocycler (Bio-Rad, Tokyo, Japan) and consisted of an initial denaturation at 98 °C for 30 s, 35 cycles of denaturation at 98 °C for 10 s, annealing at 73 °C for 30 s, and extension at 72 °C for 45 s followed by a final extension at 72 °C for 10 min.

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: Three fluorescent dyes (NED, FAM and HEX) were attached to the 5' end of the M13 universal primer sequence ( 5'- CACGACGTTGTAAAACGAC-3' ) following Schuelke [ ]. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water. .. PCR cycling conditions consisted of 2 cycles of 94°C denaturation for 2 min, 30 cycles of 94°C denaturation for 30 sec, 55°C annealing for 45 sec, and 68°C extension for 3 min, and one cycle of 68°C repair extension for 10 min. PCR products were purified (SK1141; kit Sangon Biotech), measured (3500XL sequence analyser; ABI), and sequenced.

    Article Title: Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, et al. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
    Article Snippet: Obtained sequence data were compared with those published in GenBank using BLAST. .. Polymerase chain reaction (PCR) amplification was performed in a thermocycler (iCycler, Bio‐Rad, Hercules, CA, USA) with initial denaturation at 95°C for 5 min, 33 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min and a final extension step of 7 min at 72°C.

    Pyromark Assay:

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: PCR primers were planned through PyroMark Assay Design software. .. Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water.

    DNA Extraction:

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: Paragraph title: DNA isolation, PCR amplification and genotyping of SSRs ... Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: Paragraph title: Genomic DNA extraction, genotyping, and methylation assay ... Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water.

    Article Title: Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, et al. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
    Article Snippet: Paragraph title: DNA extraction and polymerase chain reaction ... Polymerase chain reaction (PCR) amplification was performed in a thermocycler (iCycler, Bio‐Rad, Hercules, CA, USA) with initial denaturation at 95°C for 5 min, 33 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min and a final extension step of 7 min at 72°C.

    Marker:

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. Capillary electrophoresis involved multiplexed marker panels, based on expected allele size, with two to three markers with at least 80 bp size differences.

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. Capillary electrophoresis involved multiplexed marker panels, based on expected allele size, with two to three markers with at least 80 bp size differences.

    Fluorescence:

    Article Title: Studying Early Lethality of 45,XO (Turner's Syndrome) Embryos Using Human Embryonic Stem Cells
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of individual markers (fluorescence-dye-labeled forward primer and unlabeled reverse primer) was performed in a PTC 225 DNA Engine (Bio-Rad, Hercules, CA) using 25–30 ng of genomic DNA, 6 pmoles of each primer, 1.5 mM MgCl2, 0.14 mM deoxynucleoside-5-triphosphate, 1× PCR Gold Buffer and 0.4 units of AmpliTaq Gold DNA Polymerase (both from Applied Biosystems) in a total volume of 10 µl. ..

    Methylation:

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: Paragraph title: Genomic DNA extraction, genotyping, and methylation assay ... Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water.

    Isolation:

    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
    Article Snippet: Paragraph title: Isolation of canine CYP1B1 cDNA ... Briefly, polymerase chain reaction (PCR) amplification was carried out in a thermocycler (Bio-Rad, Tokyo, Japan) and consisted of an initial denaturation at 98 °C for 30 s, 35 cycles of denaturation at 98 °C for 10 s, annealing at 73 °C for 30 s, and extension at 72 °C for 45 s followed by a final extension at 72 °C for 10 min.

    Article Title: Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells
    Article Snippet: Paragraph title: RNA isolation and quantitative real-timepolymerase chain reaction ... The polymerase chain reaction (PCR) amplification was carried out using the IQ5 System (Bio-Rad) with SYBR Green Mastermix (Takara).

    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
    Article Snippet: The concentration of isolated nucleic acids was determined spectrophotometrically by measuring the absorbance at 260 nm, and the integrity was verified by GelRedTM nucleic acid gel staining (Biotium, Hayward, CA, USA) of 18S and 28S rRNA bands on a denaturing agarose gel. .. The cDNA was used as a template for polymerase chain reaction (PCR) amplification by iQ Supermix (Bio-Rad).

    Size-exclusion Chromatography:

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water. .. PCR cycling conditions consisted of 2 cycles of 94°C denaturation for 2 min, 30 cycles of 94°C denaturation for 30 sec, 55°C annealing for 45 sec, and 68°C extension for 3 min, and one cycle of 68°C repair extension for 10 min. PCR products were purified (SK1141; kit Sangon Biotech), measured (3500XL sequence analyser; ABI), and sequenced.

    Labeling:

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. PCR was carried out according to Schuelke [ ] in a two-step process as follows: the first step consisted of an initial denaturing step of 94°C for 5 min, followed by 30 cycles of 94°C for 30 s, annealing temperature ( ) for 45 s, and 72°C for 45 s. The second step consisted of 8 cycles at 94°C for 30 s, 53°C for 45 s and 72°C for 45 s, and a final extension at 72°C for 10 min. Quality of PCR products was checked by electrophoresis in agarose gels (1.5% (w/v)) stained with GelRed (Biotium) under ultraviolet light.

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. PCR was carried out according to Schuelke [ ] in a two-step process as follows: the first step consisted of an initial denaturing step of 94°C for 5 min, followed by 30 cycles of 94°C for 30 s, annealing temperature ( ) for 45 s, and 72°C for 45 s. The second step consisted of 8 cycles at 94°C for 30 s, 53°C for 45 s and 72°C for 45 s, and a final extension at 72°C for 10 min. Quality of PCR products was checked by electrophoresis in agarose gels (1.5% (w/v)) stained with GelRed (Biotium) under ultraviolet light.

    Purification:

    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
    Article Snippet: Briefly, polymerase chain reaction (PCR) amplification was carried out in a thermocycler (Bio-Rad, Tokyo, Japan) and consisted of an initial denaturation at 98 °C for 30 s, 35 cycles of denaturation at 98 °C for 10 s, annealing at 73 °C for 30 s, and extension at 72 °C for 45 s followed by a final extension at 72 °C for 10 min. .. The amplified products were purified by SUPREC-PCR (TaKaRa Bio Inc., Shiga, Japan) or Wizard SV gel and PCR clean up system (Promega, WI).

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water. .. PCR cycling conditions consisted of 2 cycles of 94°C denaturation for 2 min, 30 cycles of 94°C denaturation for 30 sec, 55°C annealing for 45 sec, and 68°C extension for 3 min, and one cycle of 68°C repair extension for 10 min. PCR products were purified (SK1141; kit Sangon Biotech), measured (3500XL sequence analyser; ABI), and sequenced.

    Polymerase Chain Reaction:

    Article Title: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
    Article Snippet: .. The polymerase chain reaction (PCR) amplification was carried out using the Step-one System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative real-time PCR (qRT-PCR) results were carried out in duplicate and normalized to GAPDH.

    Article Title: Studying Early Lethality of 45,XO (Turner's Syndrome) Embryos Using Human Embryonic Stem Cells
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of individual markers (fluorescence-dye-labeled forward primer and unlabeled reverse primer) was performed in a PTC 225 DNA Engine (Bio-Rad, Hercules, CA) using 25–30 ng of genomic DNA, 6 pmoles of each primer, 1.5 mM MgCl2, 0.14 mM deoxynucleoside-5-triphosphate, 1× PCR Gold Buffer and 0.4 units of AmpliTaq Gold DNA Polymerase (both from Applied Biosystems) in a total volume of 10 µl. ..

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. PCR was carried out according to Schuelke [ ] in a two-step process as follows: the first step consisted of an initial denaturing step of 94°C for 5 min, followed by 30 cycles of 94°C for 30 s, annealing temperature ( ) for 45 s, and 72°C for 45 s. The second step consisted of 8 cycles at 94°C for 30 s, 53°C for 45 s and 72°C for 45 s, and a final extension at 72°C for 10 min. Quality of PCR products was checked by electrophoresis in agarose gels (1.5% (w/v)) stained with GelRed (Biotium) under ultraviolet light.

    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
    Article Snippet: .. Briefly, polymerase chain reaction (PCR) amplification was carried out in a thermocycler (Bio-Rad, Tokyo, Japan) and consisted of an initial denaturation at 98 °C for 30 s, 35 cycles of denaturation at 98 °C for 10 s, annealing at 73 °C for 30 s, and extension at 72 °C for 45 s followed by a final extension at 72 °C for 10 min. .. The PCR products were electrophoresed on a 2% agarose gel and bands were visualized by ethidium bromide staining.

    Article Title: Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells
    Article Snippet: .. The polymerase chain reaction (PCR) amplification was carried out using the IQ5 System (Bio-Rad) with SYBR Green Mastermix (Takara). .. All quantitative RT-PCR tests were carried out in duplicate and normalized to β-actin.

    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
    Article Snippet: .. The cDNA was used as a template for polymerase chain reaction (PCR) amplification by iQ Supermix (Bio-Rad). ..

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas). .. PCR was carried out according to Schuelke [ ] in a two-step process as follows: the first step consisted of an initial denaturing step of 94°C for 5 min, followed by 30 cycles of 94°C for 30 s, annealing temperature ( ) for 45 s, and 72°C for 45 s. The second step consisted of 8 cycles at 94°C for 30 s, 53°C for 45 s and 72°C for 45 s, and a final extension at 72°C for 10 min. Quality of PCR products was checked by electrophoresis in agarose gels (1.5% (w/v)) stained with GelRed (Biotium) under ultraviolet light.

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
    Article Snippet: .. Total RNA Extraction and RT-PCR Analysis Total RNA was extracted from liver tissue samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized via a reverse transcriptase (RT) reaction, and polymerase chain reaction (PCR) amplification was performed using a thermal cycler (Bio-Rad, Hercules, CA, USA). .. Real-time RT-PCR analysis was carried out with a Bio-Rad CFX96TM (Bio-Rad) using iTaq™ SYBR Green SuperMix (Bio-Rad).

    Article Title: Porous nanofibrous PLLA scaffolds for vascular tissue engineering
    Article Snippet: .. Polymerase chain reaction (PCR) amplification was performed with primers for smooth muscle myosin heavy chain (SMMHC), Smoothelin and Myocardin (MyoCD) using SYBR Green supermix kit (Bio-rad, Hercules, CA) following the instructions. .. PCR primers and reaction conditions are described in .

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: .. Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water. .. PCR cycling conditions consisted of 2 cycles of 94°C denaturation for 2 min, 30 cycles of 94°C denaturation for 30 sec, 55°C annealing for 45 sec, and 68°C extension for 3 min, and one cycle of 68°C repair extension for 10 min. PCR products were purified (SK1141; kit Sangon Biotech), measured (3500XL sequence analyser; ABI), and sequenced.

    Article Title: Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, et al. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
    Article Snippet: .. Polymerase chain reaction (PCR) amplification was performed in a thermocycler (iCycler, Bio‐Rad, Hercules, CA, USA) with initial denaturation at 95°C for 5 min, 33 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min and a final extension step of 7 min at 72°C. .. Fragment sizes of amplified DNA were determined automatically by GeneScan software V3.7.

    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
    Article Snippet: .. Polymerase chain reaction (PCR) amplification of gene targets PCR reactions targeting p53, NOP14, MTMR4, ZPLD1, CDHR3, GMPR, CACNA1I, OR2S2, AGHGEF12, CACNA1C, SAMDA4, KRAS, BRAF and NGLY1 were performed on CFX ConnectTm real-time PCR machine (Bio-Rad Laboratories) using Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific) per the protocol provided in . .. All primers were synthesized by IDT (Integrated DNA Technologies, IDT) using primers depicted in .

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
    Article Snippet: Total RNA Extraction and RT-PCR Analysis Total RNA was extracted from liver tissue samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized via a reverse transcriptase (RT) reaction, and polymerase chain reaction (PCR) amplification was performed using a thermal cycler (Bio-Rad, Hercules, CA, USA). .. Primer sequences for mouse genes are as follows: modulatory subunit of GCL (GCLM), 5′-AGGAGCTTCGGGACTGTATT-3′ and 5′-TGGGCTTCAATGTCAGGGAT-3′; catalytic subunit of GCL (GCLC), 5′-CAAGGACGTGCTCAAGTGG-3′ and 5′-GTAACTCCCATACTCTGGTCTC-3′; superoxide dismutase 1 (SOD-1), 5′-GTTCCACGTCCATCAGTATG-3′ and 5′-ACACGATCTTCAATGGACAC-3′; glutathione peroxidase 1 (GPx-1), 5′-GGGACTACACCGAGATGAAC-3′ and 5′-TCACTTCGCACTTCTCAAAC-3′; glutathione reductase (GSR), 5′-CAGGCATGATAAGGTACTGAG-3′ and 5′-CATCTGGAATCATGGTCGTG-3′; catalase (CAT), 5′-CAAAGGTGTTGAACGAGGAG-3′ and 5′-TGTAGGTGTGAATTGCGTTC-3′, and hypoxanthine-guanine phosphoribosyl transferase (HPRT), 5′-AGATGTCATGAAGGAGATGG-3′ and 5′-TACAGTAGCTCTTCAGTCTG-3′.

    Software:

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: PCR primers were planned through PyroMark Assay Design software. .. Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water.

    Article Title: Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, et al. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
    Article Snippet: Polymerase chain reaction (PCR) amplification was performed in a thermocycler (iCycler, Bio‐Rad, Hercules, CA, USA) with initial denaturation at 95°C for 5 min, 33 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min and a final extension step of 7 min at 72°C. .. Fragment sizes of amplified DNA were determined automatically by GeneScan software V3.7.

    Electrophoresis:

    Article Title: Studying Early Lethality of 45,XO (Turner's Syndrome) Embryos Using Human Embryonic Stem Cells
    Article Snippet: Polymerase chain reaction (PCR) amplification of individual markers (fluorescence-dye-labeled forward primer and unlabeled reverse primer) was performed in a PTC 225 DNA Engine (Bio-Rad, Hercules, CA) using 25–30 ng of genomic DNA, 6 pmoles of each primer, 1.5 mM MgCl2, 0.14 mM deoxynucleoside-5-triphosphate, 1× PCR Gold Buffer and 0.4 units of AmpliTaq Gold DNA Polymerase (both from Applied Biosystems) in a total volume of 10 µl. .. PCR product electrophoresis and detection were performed using the 3700 Automated DNA Analyzer (Applied Biosystems).

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    RNA Extraction:

    Article Title: Inhibitory Effect of Dried Pomegranate Concentration Powder on Melanogenesis in B16F10 Melanoma Cells; Involvement of p38 and PKA Signaling Pathways
    Article Snippet: .. Total RNA Extraction and RT-PCR Analysis Total RNA was extracted from liver tissue samples using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized via a reverse transcriptase (RT) reaction, and polymerase chain reaction (PCR) amplification was performed using a thermal cycler (Bio-Rad, Hercules, CA, USA). .. Real-time RT-PCR analysis was carried out with a Bio-Rad CFX96TM (Bio-Rad) using iTaq™ SYBR Green SuperMix (Bio-Rad).

    Agarose Gel Electrophoresis:

    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
    Article Snippet: Briefly, polymerase chain reaction (PCR) amplification was carried out in a thermocycler (Bio-Rad, Tokyo, Japan) and consisted of an initial denaturation at 98 °C for 30 s, 35 cycles of denaturation at 98 °C for 10 s, annealing at 73 °C for 30 s, and extension at 72 °C for 45 s followed by a final extension at 72 °C for 10 min. .. The PCR products were electrophoresed on a 2% agarose gel and bands were visualized by ethidium bromide staining.

    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
    Article Snippet: The concentration of isolated nucleic acids was determined spectrophotometrically by measuring the absorbance at 260 nm, and the integrity was verified by GelRedTM nucleic acid gel staining (Biotium, Hayward, CA, USA) of 18S and 28S rRNA bands on a denaturing agarose gel. .. The cDNA was used as a template for polymerase chain reaction (PCR) amplification by iQ Supermix (Bio-Rad).

    DNA Methylation Assay:

    Article Title: The risk of clopidogrel resistance is associated with ABCB1 polymorphisms but not promoter methylation in a Chinese Han population
    Article Snippet: The sequences of primers used in the SNP genotyping and DNA Methylation Assay are described in . .. Polymerase chain reaction (PCR) amplification (BIO-RAD C1000touch Thermal Cycler PCR) was performed as follows: 50 μl reaction volume containing 1 μl template DNA, 1.5 μl 10 mM dNTP, 5 μl Taq Buffer, 1.0 μl25 mM MgCl2 , 1.5 μl upstream and downstream primers, 1 μl platinum Taq polymerase 1 U and 37.5 μl water.

    Concentration Assay:

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
    Article Snippet: The concentration of isolated nucleic acids was determined spectrophotometrically by measuring the absorbance at 260 nm, and the integrity was verified by GelRedTM nucleic acid gel staining (Biotium, Hayward, CA, USA) of 18S and 28S rRNA bands on a denaturing agarose gel. .. The cDNA was used as a template for polymerase chain reaction (PCR) amplification by iQ Supermix (Bio-Rad).

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    CTG Assay:

    Article Title: Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, et al. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations
    Article Snippet: Polymerase chain reaction (PCR) amplification was performed in a thermocycler (iCycler, Bio‐Rad, Hercules, CA, USA) with initial denaturation at 95°C for 5 min, 33 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min and a final extension step of 7 min at 72°C. .. Primer pairs spanning the transposable intron in the 25S rDNA as follows: CA‐INT‐L (5′‐ATA AGG GAA GTC GGC AAA ATA GAT CCG TAA‐3′) and CA‐INT‐R (5′‐CCT TGG CTG TGG TTT CGC TAG ATA GTA GAT‐3′).

    Staining:

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

    Article Title: Evaluation of the CYP1B1 gene as a candidate gene in beagles with primary open-angle glaucoma (POAG)
    Article Snippet: Briefly, polymerase chain reaction (PCR) amplification was carried out in a thermocycler (Bio-Rad, Tokyo, Japan) and consisted of an initial denaturation at 98 °C for 30 s, 35 cycles of denaturation at 98 °C for 10 s, annealing at 73 °C for 30 s, and extension at 72 °C for 45 s followed by a final extension at 72 °C for 10 min. .. The PCR products were electrophoresed on a 2% agarose gel and bands were visualized by ethidium bromide staining.

    Article Title: Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice
    Article Snippet: The concentration of isolated nucleic acids was determined spectrophotometrically by measuring the absorbance at 260 nm, and the integrity was verified by GelRedTM nucleic acid gel staining (Biotium, Hayward, CA, USA) of 18S and 28S rRNA bands on a denaturing agarose gel. .. The cDNA was used as a template for polymerase chain reaction (PCR) amplification by iQ Supermix (Bio-Rad).

    Article Title: Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia
    Article Snippet: DNA concentration was determined by comparison with known concentrations of standard DNA (lambda DNA, Invitrogen) during electrophoresis in agarose gels (1% (w/v)) stained with GelRed (Biotium) under ultraviolet light. .. Polymerase chain reaction (PCR) amplification of the DNA samples was done in a MyCycler Thermal Cycler (Bio-Rad), and performed in a final volume of 10 μL, consisting of 20 ng of DNA template, 1X PCR buffer (Fermentas, Vilnius, Lithuania), 0.25 mM of each dNTP, 1.5 mM of MgCl2 , 2.5 pmol of forward and M13 labeled primers (FAM, HEX or NED dyes), 5 pmol of reverse primers and 1 U of Taq DNA polymerase (Fermentas).

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    Bio-Rad real time pcr thermocycler
    Real Time Pcr Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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