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Eppendorf AG polymerase chain reaction mixtures
Polymerase Chain Reaction Mixtures, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction mixtures/product/Eppendorf AG
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction mixtures - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: The Determination of Genetic Markers of Age-Related Cancer Pathologies in Populations from Kazakhstan
Article Snippet: .. Twenty to one hundred nanogram of target DNA was amplified in total volume of 20 μl of PCR mixtures using amplifier “Mastercycler” (Eppendorf). .. PCR mixture contains 15 pM of each specific primer, 10 mM of each dNTP, 2 μl of 10× PCR buffer (10 mM KCl, 100 mM Tris-HCl, pH 9.0), and 0.5 U of Taq-polymerase (Sigma-Aldrich).

Article Title: Simultaneous Identification of Mycobacterial Isolates to the Species Level and Determination of Tuberculosis Drug Resistance by PCR Followed by Electrospray Ionization Mass Spectrometry ▿Simultaneous Identification of Mycobacterial Isolates to the Species Level and Determination of Tuberculosis Drug Resistance by PCR Followed by Electrospray Ionization Mass Spectrometry ▿ †
Article Snippet: .. All PCR mixtures were assembled in 40-μl volumes in the 96-well microtiter plate format by using a Packard MPII liquid handling robotic platform and a Mastercycler Pro apparatus (Eppendorf, Hauppauge, NY). .. The primers used for PCR/ESI-MS analysis are shown in .

Article Title: hsa-miR-24 suppresses metastasis in nasopharyngeal carcinoma by regulating the c-Myc/epithelial-mesenchymal transition axis
Article Snippet: .. Polymerase chain reaction mixtures containing TaqMan Universal Master Mix II (hsa-miR-24) and TaqMan miRNA and gene expression assays were performed according to the manufacturer's instructions (gradient S, Mastercycler, Eppendorf, Germany). .. To isolate c-Myc mRNA, total RNA was extracted from the cells using TRIzol reagent and reverse transcribed using a high capacity RNA-cDNA kit (Applied Biosystems Inc.). cDNA was quantified on an ABI Prism 7900 sequence detection system (Applied Biosystems Inc.).

Article Title: A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation, et al. A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation
Article Snippet: .. The target gene was amplified in a duplex in 20 μL PCR mixtures (10 μL Sybr Green, 2 μL cDNA template, 1.6 μL optimized primers, and 6.4 μL DEPC water) which was loaded into a 96‐well plate (Eppendorf White, Eppendorf, UK). .. The plate was centrifuged at 1000 rpm for 5 minutes before running the program in qPCR machine.

Article Title: Molecular Analysis of Carbon Monoxide-Oxidizing Bacteria Associated with Recent Hawaiian Volcanic Deposits †
Article Snippet: .. PCR mixtures (50 μl) contained 5 μl of 10× PCR buffer, 10 μl of Eppendorf Taqmaster buffer, 100 μM concentrations of each deoxynucleoside triphosphate, 3.5 mM magnesium ion, 0.1 μM concentrations of primers, and 1.25 U of Master Taq DNA polymerase (Brinkmann, Inc., Westbury, N.Y.). .. PCR was carried out in an Eppendorf Mastercycler thermocycler (Brinkmann, Inc.), with an initial denaturation step of 3 min at 94°C and a Taq DNA polymerase “hot start” addition at 80°C.

SYBR Green Assay:

Article Title: A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation, et al. A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation
Article Snippet: .. The target gene was amplified in a duplex in 20 μL PCR mixtures (10 μL Sybr Green, 2 μL cDNA template, 1.6 μL optimized primers, and 6.4 μL DEPC water) which was loaded into a 96‐well plate (Eppendorf White, Eppendorf, UK). .. The plate was centrifuged at 1000 rpm for 5 minutes before running the program in qPCR machine.

Amplification:

Article Title: The Determination of Genetic Markers of Age-Related Cancer Pathologies in Populations from Kazakhstan
Article Snippet: .. Twenty to one hundred nanogram of target DNA was amplified in total volume of 20 μl of PCR mixtures using amplifier “Mastercycler” (Eppendorf). .. PCR mixture contains 15 pM of each specific primer, 10 mM of each dNTP, 2 μl of 10× PCR buffer (10 mM KCl, 100 mM Tris-HCl, pH 9.0), and 0.5 U of Taq-polymerase (Sigma-Aldrich).

Article Title: A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation, et al. A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation
Article Snippet: .. The target gene was amplified in a duplex in 20 μL PCR mixtures (10 μL Sybr Green, 2 μL cDNA template, 1.6 μL optimized primers, and 6.4 μL DEPC water) which was loaded into a 96‐well plate (Eppendorf White, Eppendorf, UK). .. The plate was centrifuged at 1000 rpm for 5 minutes before running the program in qPCR machine.

Nested PCR:

Article Title: Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts ▿
Article Snippet: .. Both external and nested PCR mixtures were subjected to an initial temperature of 94°C for 3 min, followed by 40 cycles of 94°C for 20 s, 50°C for 10 s, and 72°C for 1 min, and a final extension period of 72°C for 10 min in an Eppendorf Mastercycler gradient. .. PCR products and a 100-bp ladder were visualized on a 1% ethidium bromide-stained agarose gel for confirmation of amplification.

Expressing:

Article Title: hsa-miR-24 suppresses metastasis in nasopharyngeal carcinoma by regulating the c-Myc/epithelial-mesenchymal transition axis
Article Snippet: .. Polymerase chain reaction mixtures containing TaqMan Universal Master Mix II (hsa-miR-24) and TaqMan miRNA and gene expression assays were performed according to the manufacturer's instructions (gradient S, Mastercycler, Eppendorf, Germany). .. To isolate c-Myc mRNA, total RNA was extracted from the cells using TRIzol reagent and reverse transcribed using a high capacity RNA-cDNA kit (Applied Biosystems Inc.). cDNA was quantified on an ABI Prism 7900 sequence detection system (Applied Biosystems Inc.).

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    Eppendorf AG polymerase chain reaction mixtures
    Polymerase Chain Reaction Mixtures, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction mixtures/product/Eppendorf AG
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction mixtures - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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