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Bio-Rad polymerase chain reaction mixtures
Polymerase Chain Reaction Mixtures, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polymerase chain reaction mixtures/product/Bio-Rad
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polymerase chain reaction mixtures - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection
Article Snippet: .. The 50 µL of PCR mixtures contained 10 µL 5× HF reaction buffer, 0.4 U of iProof polymerase (Bio-Rad), 500 nM forward primer, 500 nM biotinylated reverse primer, 0.4 mM CleanAmp dNTP (Trilink, San Diego, CA, USA), and 10 µg mL−1 BSA. ..

Article Title: Genomewide Identification of Essential Genes and Fitness Determinants of Streptococcus mutans UA159
Article Snippet: .. PCR mixtures (20 µl) were run on a Bio-Rad CFX96 thermocycler using SsoAdvanced Universal SYBR green supermix (Bio-Rad) as follows: (i) 3 min at 98°C; (ii) 40 cycles, with 1 cycle consisting of 15 s at 98°C and 45 s at 68°C. .. To estimate the total number of bacteria, we developed degenerate primers to conserved regions of the ubiquitous single-copy gene rpsL (30S ribosomal protein S12) ( ) after ClustalW alignment (MacVector v.12) of sequences from 22 different species of oral and nonoral streptococci, two strains of Lactobacillus murinus (ASF361 and DSM20452), two species of Staphylococcus ( Staphylococcus saprophyticus 772 and Staphylococcus xylosus HKUOPL8), three species of Corynebacterium ( Corynebacterium pilosum CIP103422, Corynebacterium renale CIP52.96, and Corynebacterium mastitidis DSM44356), and Muribacter muris Ackerman 80-443-D.

Article Title: Poecivirus is present in individuals with beak deformities in seven species of North American birds
Article Snippet: .. PCR mixtures contained 1x iProof master mix (Bio-Rad Laboratories, Hercules, CA, USA), 0.5 M primer, and 5µl cDNA. ..

Article Title: Impact of Different In Vitro Electron Donor/Acceptor Conditions on Potential Chemolithoautotrophic Communities from Marine Pelagic Redoxclines
Article Snippet: .. The PCR mixtures (25 μl) contained 1× iQSybrGreen Supermix (Bio-Rad), bacterial Com (900 nmol liter−1 each) or strain-specific OST 1 real-time primer system (OST 1F, 900 nmol liter−1 ; OST 1R, 300 nmol liter−1 ) ( , ), and 3 ng of nucleic acids. ..

Article Title: RNA-Seq Analysis of a Soybean Near-Isogenic Line Carrying Bacterial Leaf Pustule-Resistant and -Susceptible Alleles
Article Snippet: .. PCR mixtures (final volume, 50 µl) contained 200 ng of cDNA, 500 nM each primer, 18 µl of sterile water, and 25 µl of iQ™ SYBR Green Supermix (Bio-Rad). .. The conditions for amplification were as follows: 5 min denaturation at 95°C followed by 40 cycles of 95°C for 10 s, 60°C for 20 s, and 72°C for 10 s. Following amplification, melting curves were determined in a three-segment cycle of 95°C for 5 s, 65°C for 1 min, and 97°C for 0 s on the continuous acquisition mode.

Article Title: Ex vivo expansion of cord blood progenitors impairs their short‐term and long‐term repopulating activity associated with transcriptional dysregulation of signalling networks
Article Snippet: .. PCR mixtures (25 μl per reaction) contained cDNA, 0.2 μmol/l each of forward and reverse primers, and 12.5 μl iQ SYBR Green Supermix (Bio‐Rad, Hercules, CA, USA). .. Reactions were performed in triplicate in 96‐well plates using the iCycler iQ Real‐Time PCR Detection System version 4.006 (Bio‐Rad), and were analysed using software from the manufacturer.

Article Title: Snail Family Members Unequally Trigger EMT and Thereby Differ in Their Ability to Promote the Neoplastic Transformation of Mammary Epithelial Cells
Article Snippet: .. PCR mixtures contained SsoAdvanced SYBR Green supermix (1725264, Biorad) and 200 nM primers. ..

Article Title: Seasonal and Regional Dynamics of M. ulcerans Transmission in Environmental Context: Deciphering the Role of Water Bugs as Hosts and Vectors
Article Snippet: .. PCR mixtures contained 5 µl of template DNA, 0.3 µM concentration of each primer, 0.25 µM concentration of the probe, and IQSupermix (Bio-Rad Lab) in a total volume of 25 µl. .. Amplification and detection were performed with Thermocycler (MX3000P, Stratagene) using the following program: 1 cycle of 50°C for 2 min, 1 cycle of 95°C for 15 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. DNA extracts were tested at least as duplicates, and negative controls were included in each assay.

SYBR Green Assay:

Article Title: Genomewide Identification of Essential Genes and Fitness Determinants of Streptococcus mutans UA159
Article Snippet: .. PCR mixtures (20 µl) were run on a Bio-Rad CFX96 thermocycler using SsoAdvanced Universal SYBR green supermix (Bio-Rad) as follows: (i) 3 min at 98°C; (ii) 40 cycles, with 1 cycle consisting of 15 s at 98°C and 45 s at 68°C. .. To estimate the total number of bacteria, we developed degenerate primers to conserved regions of the ubiquitous single-copy gene rpsL (30S ribosomal protein S12) ( ) after ClustalW alignment (MacVector v.12) of sequences from 22 different species of oral and nonoral streptococci, two strains of Lactobacillus murinus (ASF361 and DSM20452), two species of Staphylococcus ( Staphylococcus saprophyticus 772 and Staphylococcus xylosus HKUOPL8), three species of Corynebacterium ( Corynebacterium pilosum CIP103422, Corynebacterium renale CIP52.96, and Corynebacterium mastitidis DSM44356), and Muribacter muris Ackerman 80-443-D.

Article Title: RNA-Seq Analysis of a Soybean Near-Isogenic Line Carrying Bacterial Leaf Pustule-Resistant and -Susceptible Alleles
Article Snippet: .. PCR mixtures (final volume, 50 µl) contained 200 ng of cDNA, 500 nM each primer, 18 µl of sterile water, and 25 µl of iQ™ SYBR Green Supermix (Bio-Rad). .. The conditions for amplification were as follows: 5 min denaturation at 95°C followed by 40 cycles of 95°C for 10 s, 60°C for 20 s, and 72°C for 10 s. Following amplification, melting curves were determined in a three-segment cycle of 95°C for 5 s, 65°C for 1 min, and 97°C for 0 s on the continuous acquisition mode.

Article Title: Ex vivo expansion of cord blood progenitors impairs their short‐term and long‐term repopulating activity associated with transcriptional dysregulation of signalling networks
Article Snippet: .. PCR mixtures (25 μl per reaction) contained cDNA, 0.2 μmol/l each of forward and reverse primers, and 12.5 μl iQ SYBR Green Supermix (Bio‐Rad, Hercules, CA, USA). .. Reactions were performed in triplicate in 96‐well plates using the iCycler iQ Real‐Time PCR Detection System version 4.006 (Bio‐Rad), and were analysed using software from the manufacturer.

Article Title: Snail Family Members Unequally Trigger EMT and Thereby Differ in Their Ability to Promote the Neoplastic Transformation of Mammary Epithelial Cells
Article Snippet: .. PCR mixtures contained SsoAdvanced SYBR Green supermix (1725264, Biorad) and 200 nM primers. ..

Concentration Assay:

Article Title: Seasonal and Regional Dynamics of M. ulcerans Transmission in Environmental Context: Deciphering the Role of Water Bugs as Hosts and Vectors
Article Snippet: .. PCR mixtures contained 5 µl of template DNA, 0.3 µM concentration of each primer, 0.25 µM concentration of the probe, and IQSupermix (Bio-Rad Lab) in a total volume of 25 µl. .. Amplification and detection were performed with Thermocycler (MX3000P, Stratagene) using the following program: 1 cycle of 50°C for 2 min, 1 cycle of 95°C for 15 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. DNA extracts were tested at least as duplicates, and negative controls were included in each assay.

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    Bio-Rad polymerase chain reaction mixture
    Polymerase Chain Reaction Mixture, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction mixture/product/Bio-Rad
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction mixture - by Bioz Stars, 2020-09
    90/100 stars
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