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Poly Scientific Inc polyethylenimine
Polyethylenimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethylenimine/product/Poly Scientific Inc
Average 92 stars, based on 49 article reviews
Price from $9.99 to $1999.99
polyethylenimine - by Bioz Stars, 2021-01
92/100 stars

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Transfection:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Article Title: LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Article Snippet: .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands. .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands.

Article Title: Epigallocatechin-3-Gallate (EGCG)-Inducible SMILE Inhibits STAT3-Mediated Hepcidin Gene Expression
Article Snippet: .. Transient transfections were carried out using polyethylenimine (Polysciences, Inc., Warrington, PA, USA) or SuperFect (QIAGEN, Hilden, Germany), according to manufacturer’s instruction. .. Luciferase assay was performed as described previously [ ].

Article Title: Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H
Article Snippet: .. For transient transfections, 20 μl of polyethylenimine (PEI, 1 mg/ml) (24765, Polysciences Inc) and 2 μg of plasmid DNA was added to 1 mL of Opti-Mem (31985–062, GIBCO). .. This mixture was then gently vortexed for 20 s then incubated at room temperature for 20 min. After incubation, the transfection mixture was added drop by drop to the media of cells in a 10 cm dish.

Article Title: Visualization of clustered protocadherin neuronal self-recognition complexes
Article Snippet: .. The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.). .. Media was harvested ~6 days after transfection and the secreted proteins were purified by nickel affinity chromatography followed by size exclusion chromatography in 10 mM Tris pH 8.0, 150 mM sodium chloride, 3 mM calcium chloride, and 200–250 mM imidazole pH 8.0.

Article Title: GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion
Article Snippet: .. Polyethylenimine was used for transient transfection of plasmids according to manufacturer’s instructions (Polysciences, Inc., 23966–1). .. For knockdown experiments, HeLa cells or mRFP-GFP-LC3 expressing cells were transfected with Lipofectamine RNAiMAX according to manufacturer’s instructions (Thermo Fisher Scientific, 13778150) and cells were used 72 h after transfection.

Luciferase:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Mutagenesis:

Article Title: A De Novo Frameshift Mutation in TNFAIP3 Impairs A20 Deubiquitination Function to Cause Neuropsychiatric Systemic Lupus Erythematosus.
Article Snippet: .. Genome-wide association study of systemic lupus erythematosus (SLE) revealed tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20) as a susceptibility gene. .. Genome-wide association study of systemic lupus erythematosus (SLE) revealed tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20) as a susceptibility gene.

Knock-Out:

Article Title: In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Article Snippet: .. Briefly, the Control or Knockout plasmid, AAV2 serotype–specific packaging plasmid, and helper plasmid, in a ratio of 1:1:3, were mixed with polyethyleneimine (PEI; Polysciences Inc.) and incubated for 10 min to form complexes. .. The transfection complexes were added to HEK293T cells and left for 72 h. The cells were harvested and lysed by freeze and thaw (3×) in PBS.

Cell Culture:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Incubation:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Article Title: In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Article Snippet: .. Briefly, the Control or Knockout plasmid, AAV2 serotype–specific packaging plasmid, and helper plasmid, in a ratio of 1:1:3, were mixed with polyethyleneimine (PEI; Polysciences Inc.) and incubated for 10 min to form complexes. .. The transfection complexes were added to HEK293T cells and left for 72 h. The cells were harvested and lysed by freeze and thaw (3×) in PBS.

Construct:

Article Title: Visualization of clustered protocadherin neuronal self-recognition complexes
Article Snippet: .. The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.). .. Media was harvested ~6 days after transfection and the secreted proteins were purified by nickel affinity chromatography followed by size exclusion chromatography in 10 mM Tris pH 8.0, 150 mM sodium chloride, 3 mM calcium chloride, and 200–250 mM imidazole pH 8.0.

Lysis:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Recombinant:

Article Title: LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Article Snippet: .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands. .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands.

Plasmid Preparation:

Article Title: Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H
Article Snippet: .. For transient transfections, 20 μl of polyethylenimine (PEI, 1 mg/ml) (24765, Polysciences Inc) and 2 μg of plasmid DNA was added to 1 mL of Opti-Mem (31985–062, GIBCO). .. This mixture was then gently vortexed for 20 s then incubated at room temperature for 20 min. After incubation, the transfection mixture was added drop by drop to the media of cells in a 10 cm dish.

Article Title: In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Article Snippet: .. Briefly, the Control or Knockout plasmid, AAV2 serotype–specific packaging plasmid, and helper plasmid, in a ratio of 1:1:3, were mixed with polyethyleneimine (PEI; Polysciences Inc.) and incubated for 10 min to form complexes. .. The transfection complexes were added to HEK293T cells and left for 72 h. The cells were harvested and lysed by freeze and thaw (3×) in PBS.

Article Title: Visualization of clustered protocadherin neuronal self-recognition complexes
Article Snippet: .. The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.). .. Media was harvested ~6 days after transfection and the secreted proteins were purified by nickel affinity chromatography followed by size exclusion chromatography in 10 mM Tris pH 8.0, 150 mM sodium chloride, 3 mM calcium chloride, and 200–250 mM imidazole pH 8.0.

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  • 92
    Poly Scientific Inc polyethylenimine pei
    Polyethylenimine Pei, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethylenimine pei/product/Poly Scientific Inc
    Average 92 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine pei - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    92
    Poly Scientific Inc polyethylenimine
    Polyethylenimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethylenimine/product/Poly Scientific Inc
    Average 92 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    88
    Poly Scientific Inc 25 kda linear polyethylenimine pei
    25 Kda Linear Polyethylenimine Pei, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/25 kda linear polyethylenimine pei/product/Poly Scientific Inc
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    25 kda linear polyethylenimine pei - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

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