Structured Review

Poly Scientific Inc polyethylenimine
Polyethylenimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethylenimine/product/Poly Scientific Inc
Average 93 stars, based on 49 article reviews
Price from $9.99 to $1999.99
polyethylenimine - by Bioz Stars, 2020-08
93/100 stars

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Transfection:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Article Title: LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Article Snippet: .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands. .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands.

Article Title: Epigallocatechin-3-Gallate (EGCG)-Inducible SMILE Inhibits STAT3-Mediated Hepcidin Gene Expression
Article Snippet: .. Transient transfections were carried out using polyethylenimine (Polysciences, Inc., Warrington, PA, USA) or SuperFect (QIAGEN, Hilden, Germany), according to manufacturer’s instruction. .. Luciferase assay was performed as described previously [ ].

Article Title: Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H
Article Snippet: .. For transient transfections, 20 μl of polyethylenimine (PEI, 1 mg/ml) (24765, Polysciences Inc) and 2 μg of plasmid DNA was added to 1 mL of Opti-Mem (31985–062, GIBCO). .. This mixture was then gently vortexed for 20 s then incubated at room temperature for 20 min. After incubation, the transfection mixture was added drop by drop to the media of cells in a 10 cm dish.

Article Title: Identifying triplex binding rules in vitro leads to creation of a new synthetic regulatory tool in vivo
Article Snippet: .. 24h post seeding, cells were transfected using polyethylenimine (PEI, from Polysciences, Inc. cat. no. 23966-1) in OptiMEM (Gibco, cat. no. 31985070). .. PEI stock concentration was 1 mg/ml, in ultrapure water.

Article Title: Visualization of clustered protocadherin neuronal self-recognition complexes
Article Snippet: .. The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.). .. Media was harvested ~6 days after transfection and the secreted proteins were purified by nickel affinity chromatography followed by size exclusion chromatography in 10 mM Tris pH 8.0, 150 mM sodium chloride, 3 mM calcium chloride, and 200–250 mM imidazole pH 8.0.

Article Title: GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion
Article Snippet: .. Polyethylenimine was used for transient transfection of plasmids according to manufacturer’s instructions (Polysciences, Inc., 23966–1). .. For knockdown experiments, HeLa cells or mRFP-GFP-LC3 expressing cells were transfected with Lipofectamine RNAiMAX according to manufacturer’s instructions (Thermo Fisher Scientific, 13778150) and cells were used 72 h after transfection.

Luciferase:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Construct:

Article Title: Visualization of clustered protocadherin neuronal self-recognition complexes
Article Snippet: .. The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.). .. Media was harvested ~6 days after transfection and the secreted proteins were purified by nickel affinity chromatography followed by size exclusion chromatography in 10 mM Tris pH 8.0, 150 mM sodium chloride, 3 mM calcium chloride, and 200–250 mM imidazole pH 8.0.

Knock-Out:

Article Title: In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Article Snippet: .. Briefly, the Control or Knockout plasmid, AAV2 serotype–specific packaging plasmid, and helper plasmid, in a ratio of 1:1:3, were mixed with polyethyleneimine (PEI; Polysciences Inc.) and incubated for 10 min to form complexes. .. The transfection complexes were added to HEK293T cells and left for 72 h. The cells were harvested and lysed by freeze and thaw (3×) in PBS.

Incubation:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Article Title: In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Article Snippet: .. Briefly, the Control or Knockout plasmid, AAV2 serotype–specific packaging plasmid, and helper plasmid, in a ratio of 1:1:3, were mixed with polyethyleneimine (PEI; Polysciences Inc.) and incubated for 10 min to form complexes. .. The transfection complexes were added to HEK293T cells and left for 72 h. The cells were harvested and lysed by freeze and thaw (3×) in PBS.

Cell Culture:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Lysis:

Article Title: Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2 (IRP2)
Article Snippet: .. SW480 or HepG2 cells were transfected with these reporter plasmids with Polyethylenimine (linear, ~25kDa, Polysciences, Inc) and treated with cisplatin, transplatin, carboplatin, PtCl2 , FAC, or DFO for 18–24 h. Cell lysates in luciferase cell culture lysis reagent (Promega, Madison, WI) were incubated with the luciferase assay substrate (E1501; Promega). .. Luciferase activates were measured by GloMax 20/20 (Promega).

Recombinant:

Article Title: LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Article Snippet: .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands. .. Presynaptic neurexins (Nrxs) and type IIa receptor-type protein tyrosine phosphatases (RPTPs) organize synapses through a network of postsynaptic ligands.

Plasmid Preparation:

Article Title: In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Article Snippet: .. Briefly, the Control or Knockout plasmid, AAV2 serotype–specific packaging plasmid, and helper plasmid, in a ratio of 1:1:3, were mixed with polyethyleneimine (PEI; Polysciences Inc.) and incubated for 10 min to form complexes. .. The transfection complexes were added to HEK293T cells and left for 72 h. The cells were harvested and lysed by freeze and thaw (3×) in PBS.

Article Title: Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H
Article Snippet: .. For transient transfections, 20 μl of polyethylenimine (PEI, 1 mg/ml) (24765, Polysciences Inc) and 2 μg of plasmid DNA was added to 1 mL of Opti-Mem (31985–062, GIBCO). .. This mixture was then gently vortexed for 20 s then incubated at room temperature for 20 min. After incubation, the transfection mixture was added drop by drop to the media of cells in a 10 cm dish.

Article Title: Visualization of clustered protocadherin neuronal self-recognition complexes
Article Snippet: .. The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.). .. Media was harvested ~6 days after transfection and the secreted proteins were purified by nickel affinity chromatography followed by size exclusion chromatography in 10 mM Tris pH 8.0, 150 mM sodium chloride, 3 mM calcium chloride, and 200–250 mM imidazole pH 8.0.

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  • 91
    Poly Scientific Inc polyethyleneimine pei
    Polyethyleneimine Pei, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine pei/product/Poly Scientific Inc
    Average 91 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine pei - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    Poly Scientific Inc polyethylenimine
    Polyethylenimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethylenimine/product/Poly Scientific Inc
    Average 93 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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