polyethylenimine  (Millipore)


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  • 99
    Name:
    Polyethylenimine 80 ethoxylated solution
    Description:

    Catalog Number:
    306185
    Price:
    None
    Applications:
    Additive to coatings and printing inks, brightener in electroplating, cationic modifier in clays and starches and adhesion/substantivity promoter.
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    Structured Review

    Millipore polyethylenimine
    Polyethylenimine 80 ethoxylated solution

    https://www.bioz.com/result/polyethylenimine/product/Millipore
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy"

    Article Title: Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S166445

    Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RBC, red blood cell; RH, rhein.
    Figure Legend Snippet: Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RBC, red blood cell; RH, rhein.

    Techniques Used: Hemolysis Assay, Flow Cytometry, Cytometry, Fluorescence

    GPC spectra of the polymers ( A ) and the fluorescent scanning spectra of Cy5-labeled polymers ( B ). Fluorescence spectra of pyrene in water-containing series of concentrations of PEG-PCL ( C ) and PEG-PCL-PEI ( D ). The CMC values of PEG-PCL ( E ) and PEG-PCL-PEI ( F ) were calculated from intersection between the fitting curves of I 338 /I 333 vs concentrations and the fitting curves of fluorescence intensity vs concentrations. Abbreviations: CMC, critical micelle concentration; Cy5, cyanine 5; dRI, differential refractive index; GPC, gel permeation chromatography; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RFU, relative fluorescence unit.
    Figure Legend Snippet: GPC spectra of the polymers ( A ) and the fluorescent scanning spectra of Cy5-labeled polymers ( B ). Fluorescence spectra of pyrene in water-containing series of concentrations of PEG-PCL ( C ) and PEG-PCL-PEI ( D ). The CMC values of PEG-PCL ( E ) and PEG-PCL-PEI ( F ) were calculated from intersection between the fitting curves of I 338 /I 333 vs concentrations and the fitting curves of fluorescence intensity vs concentrations. Abbreviations: CMC, critical micelle concentration; Cy5, cyanine 5; dRI, differential refractive index; GPC, gel permeation chromatography; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RFU, relative fluorescence unit.

    Techniques Used: Gel Permeation Chromatography, Labeling, Fluorescence, Concentration Assay, GPC Assay

    The synthesis process of the PEG-PCL-PEI polymer ( A ) and the 1 H-NMR spectra of the polymers: ( B ) PEG-PCL, ( C ) PEG-PCL-COCHCH 2 , ( D ) PEI, ( E ) PEG-PCL-PEI. Note: Dotted circle represented as enlarged position. Abbreviations: DMF, N , N -dimethylformamide; NMR, nuclear magnetic resonance; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; PEI, polyethylenimine.
    Figure Legend Snippet: The synthesis process of the PEG-PCL-PEI polymer ( A ) and the 1 H-NMR spectra of the polymers: ( B ) PEG-PCL, ( C ) PEG-PCL-COCHCH 2 , ( D ) PEI, ( E ) PEG-PCL-PEI. Note: Dotted circle represented as enlarged position. Abbreviations: DMF, N , N -dimethylformamide; NMR, nuclear magnetic resonance; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; PEI, polyethylenimine.

    Techniques Used: Nuclear Magnetic Resonance

    2) Product Images from "Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy"

    Article Title: Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S166445

    The morphology of nanoparticles: ( A ) a, water; b, PPP-NPs; c, PPP Cy5 -NPs; d, PPP Cy5 -RH-NPs; e, PPP-RH-NPs. TEM photograph, magnification ×100,000 ( B , C ) and size distribution ( D , E ) of PPP-RH-NPs without, or with, high-pressure homogenization treatment. Scale bar is 200 nm. PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH; PPP-RH-NPs, RH-loaded PEG-PCL-PEI nanoparticles. Abbreviations: Cy5, cyanine 5; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RH, rhein; TEM, transmission electron microscopy.
    Figure Legend Snippet: The morphology of nanoparticles: ( A ) a, water; b, PPP-NPs; c, PPP Cy5 -NPs; d, PPP Cy5 -RH-NPs; e, PPP-RH-NPs. TEM photograph, magnification ×100,000 ( B , C ) and size distribution ( D , E ) of PPP-RH-NPs without, or with, high-pressure homogenization treatment. Scale bar is 200 nm. PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH; PPP-RH-NPs, RH-loaded PEG-PCL-PEI nanoparticles. Abbreviations: Cy5, cyanine 5; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RH, rhein; TEM, transmission electron microscopy.

    Techniques Used: Transmission Electron Microscopy, Homogenization, Transmission Assay, Electron Microscopy

    Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RBC, red blood cell; RH, rhein.
    Figure Legend Snippet: Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RBC, red blood cell; RH, rhein.

    Techniques Used: Hemolysis Assay, Flow Cytometry, Cytometry, Fluorescence

    GPC spectra of the polymers ( A ) and the fluorescent scanning spectra of Cy5-labeled polymers ( B ). Fluorescence spectra of pyrene in water-containing series of concentrations of PEG-PCL ( C ) and PEG-PCL-PEI ( D ). The CMC values of PEG-PCL ( E ) and PEG-PCL-PEI ( F ) were calculated from intersection between the fitting curves of I 338 /I 333 vs concentrations and the fitting curves of fluorescence intensity vs concentrations. Abbreviations: CMC, critical micelle concentration; Cy5, cyanine 5; dRI, differential refractive index; GPC, gel permeation chromatography; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RFU, relative fluorescence unit.
    Figure Legend Snippet: GPC spectra of the polymers ( A ) and the fluorescent scanning spectra of Cy5-labeled polymers ( B ). Fluorescence spectra of pyrene in water-containing series of concentrations of PEG-PCL ( C ) and PEG-PCL-PEI ( D ). The CMC values of PEG-PCL ( E ) and PEG-PCL-PEI ( F ) were calculated from intersection between the fitting curves of I 338 /I 333 vs concentrations and the fitting curves of fluorescence intensity vs concentrations. Abbreviations: CMC, critical micelle concentration; Cy5, cyanine 5; dRI, differential refractive index; GPC, gel permeation chromatography; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RFU, relative fluorescence unit.

    Techniques Used: Gel Permeation Chromatography, Labeling, Fluorescence, Concentration Assay, GPC Assay

    The synthesis process of the PEG-PCL-PEI polymer ( A ) and the 1 H-NMR spectra of the polymers: ( B ) PEG-PCL, ( C ) PEG-PCL-COCHCH 2 , ( D ) PEI, ( E ) PEG-PCL-PEI. Note: Dotted circle represented as enlarged position. Abbreviations: DMF, N , N -dimethylformamide; NMR, nuclear magnetic resonance; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; PEI, polyethylenimine.
    Figure Legend Snippet: The synthesis process of the PEG-PCL-PEI polymer ( A ) and the 1 H-NMR spectra of the polymers: ( B ) PEG-PCL, ( C ) PEG-PCL-COCHCH 2 , ( D ) PEI, ( E ) PEG-PCL-PEI. Note: Dotted circle represented as enlarged position. Abbreviations: DMF, N , N -dimethylformamide; NMR, nuclear magnetic resonance; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; PEI, polyethylenimine.

    Techniques Used: Nuclear Magnetic Resonance

    In vitro release profiles of PP-RH-NPs, PPP-RH-NPs and RH-sol in PBS (pH 7.4). Notes: PP-RH-NPs, RH-loaded PEG-PCL nanoparticles; PPP-RH-NPs, RH-loaded PEG-PCL-PEI nanoparticles. Abbreviations: PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RH, rhein; RH-sol, RH solution.
    Figure Legend Snippet: In vitro release profiles of PP-RH-NPs, PPP-RH-NPs and RH-sol in PBS (pH 7.4). Notes: PP-RH-NPs, RH-loaded PEG-PCL nanoparticles; PPP-RH-NPs, RH-loaded PEG-PCL-PEI nanoparticles. Abbreviations: PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RH, rhein; RH-sol, RH solution.

    Techniques Used: In Vitro

    3) Product Images from "Calcium condensation of DNA complexed with cell-penetrating peptides offers efficient, noncytotoxic gene delivery"

    Article Title: Calcium condensation of DNA complexed with cell-penetrating peptides offers efficient, noncytotoxic gene delivery

    Journal: Journal of pharmaceutical sciences

    doi: 10.1002/jps.22407

    Cytotoxicity profiles of PEI andCPPs.. Viability is expressed as a function of polymer concentration. Results are presented as mean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides; Arg7, arginine 7; Arg9, arginine 9; Ahp,
    Figure Legend Snippet: Cytotoxicity profiles of PEI andCPPs.. Viability is expressed as a function of polymer concentration. Results are presented as mean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides; Arg7, arginine 7; Arg9, arginine 9; Ahp,

    Techniques Used: Concentration Assay

    The diameter of CPPs–Ca/pGL3 and PEI complexes (without and with 113 mM CaCl2) in (a) the presence and (b) absence of 10% fetal bovine serum. Results are presented as mean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides;
    Figure Legend Snippet: The diameter of CPPs–Ca/pGL3 and PEI complexes (without and with 113 mM CaCl2) in (a) the presence and (b) absence of 10% fetal bovine serum. Results are presented as mean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides;

    Techniques Used:

    The transfection efficiency of CPP polyplexes in the absence and presence of 10% fetal bovine serum. Results are presented asmean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides; Arg7, arginine 7; Arg9, arginine 9; Ahp, antennapedia
    Figure Legend Snippet: The transfection efficiency of CPP polyplexes in the absence and presence of 10% fetal bovine serum. Results are presented asmean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides; Arg7, arginine 7; Arg9, arginine 9; Ahp, antennapedia

    Techniques Used: Transfection, Conditioned Place Preference

    The effect of CaCl2 (113 mM) on the charge of PEI and CPPs complexes. Results are presented as mean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides; Arg7, arginine 7; Arg9, arginine 9; Ahp, antennapedia heptapeptide; Alp,
    Figure Legend Snippet: The effect of CaCl2 (113 mM) on the charge of PEI and CPPs complexes. Results are presented as mean ± SD (n = 3). PEI, polyethylenimine; CPPs, cell-penetrating peptides; Arg7, arginine 7; Arg9, arginine 9; Ahp, antennapedia heptapeptide; Alp,

    Techniques Used: ALP Assay

    4) Product Images from "Highly effective antiangiogenesis via magnetic mesoporous silica-based siRNA vehicle targeting the VEGF gene for orthotopic ovarian cancer therapy"

    Article Title: Highly effective antiangiogenesis via magnetic mesoporous silica-based siRNA vehicle targeting the VEGF gene for orthotopic ovarian cancer therapy

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S78774

    ( A ) Orthotopic ovarian mouse tumor (red circle is the tumor position). ( B ) In vivo T 2 -weighted magnetic resonance images of the xenograft tumor model before (left) and 24 hours after (right) injection of M-MSN_NC siRNA@PEI-PEG-KALA. Notes: Gray-scale images (top, I and II) and pseudocolor images (bottom, I’ and II’) are shown. Abbreviations: M-MSN, magnetic mesoporous silica nanoparticles; PEI, polyethylenimine; PEG, polyethylene glycol; KALA, a type of fusogenic peptide; NC siRNA, negative control small interfering RNA.
    Figure Legend Snippet: ( A ) Orthotopic ovarian mouse tumor (red circle is the tumor position). ( B ) In vivo T 2 -weighted magnetic resonance images of the xenograft tumor model before (left) and 24 hours after (right) injection of M-MSN_NC siRNA@PEI-PEG-KALA. Notes: Gray-scale images (top, I and II) and pseudocolor images (bottom, I’ and II’) are shown. Abbreviations: M-MSN, magnetic mesoporous silica nanoparticles; PEI, polyethylenimine; PEG, polyethylene glycol; KALA, a type of fusogenic peptide; NC siRNA, negative control small interfering RNA.

    Techniques Used: In Vivo, Injection, Negative Control, Small Interfering RNA

    ( A ) Transmission electron microscopic image of M-MSN_siRNA@PEI-PEG-KALA. ( B ) Dynamic light scattering measurements of size distribution for M-MSN_siRNA@PEI-PEG-KALA (dispersed in saline). ( C ) Zeta potential of M-MSN_siRNA@PEI-PEG-KALA. ( D ) T 2 relaxation rate (1/ T 2 ) as a function of iron concentration for M-MSN_siRNA@PEI-PEG-KALA. Notes: Scale bar, 20 nm. ( B ) The polydispersity index (PDI) of this nanocarrier was 0.095. Abbreviations: M-MSN, magnetic mesoporous silica nanoparticle; siRNA, small interfering RNA; PEI, polyethylenimine; PEG, polyethylene glycol; KALA, a type of fusogenic peptide.
    Figure Legend Snippet: ( A ) Transmission electron microscopic image of M-MSN_siRNA@PEI-PEG-KALA. ( B ) Dynamic light scattering measurements of size distribution for M-MSN_siRNA@PEI-PEG-KALA (dispersed in saline). ( C ) Zeta potential of M-MSN_siRNA@PEI-PEG-KALA. ( D ) T 2 relaxation rate (1/ T 2 ) as a function of iron concentration for M-MSN_siRNA@PEI-PEG-KALA. Notes: Scale bar, 20 nm. ( B ) The polydispersity index (PDI) of this nanocarrier was 0.095. Abbreviations: M-MSN, magnetic mesoporous silica nanoparticle; siRNA, small interfering RNA; PEI, polyethylenimine; PEG, polyethylene glycol; KALA, a type of fusogenic peptide.

    Techniques Used: Transmission Assay, Concentration Assay, Small Interfering RNA

    Schematic representation of the design of this study. Note: Synthesis of M-MSN_siRNA@PEI-PEG-KALA (top) and systemic administration of VEGF siRNA via this nanocarrier into orthotopic ovarian tumor-bearing mice led to effective silencing of VEGF gene expression in cancer cells and inhibited angiogenesis, ultimately leading to suppression of cancer growth. Abbreviations: M-MSN, magnetic mesoporous silica nanoparticle; siRNA, small interfering RNA; PDI, polydispersity index; PEI, polyethylenimine; PEG, polyethylene glycol; KALA, a type of fusogenic peptide; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Schematic representation of the design of this study. Note: Synthesis of M-MSN_siRNA@PEI-PEG-KALA (top) and systemic administration of VEGF siRNA via this nanocarrier into orthotopic ovarian tumor-bearing mice led to effective silencing of VEGF gene expression in cancer cells and inhibited angiogenesis, ultimately leading to suppression of cancer growth. Abbreviations: M-MSN, magnetic mesoporous silica nanoparticle; siRNA, small interfering RNA; PDI, polydispersity index; PEI, polyethylenimine; PEG, polyethylene glycol; KALA, a type of fusogenic peptide; VEGF, vascular endothelial growth factor.

    Techniques Used: Mouse Assay, Expressing, Small Interfering RNA

    5) Product Images from "Visualization of MMP-2 Activity Using Dual-Probe Nanoparticles to Detect Potential Metastatic Cancer Cells"

    Article Title: Visualization of MMP-2 Activity Using Dual-Probe Nanoparticles to Detect Potential Metastatic Cancer Cells

    Journal: Nanomaterials

    doi: 10.3390/nano8020119

    Schematic illustration of the matrix metalloproteinase-2 (MMP-2)-activated peptide sensor and MMP-2-activated poly(lactic-co-glycolic acid) with polyethylenimine (MMP-2-PLGA-PEI) nanoparticles. ( A ) Chemical structure of the MMP-2-activated peptide sensor. The sensor consisted of a near-infrared fluorescence dye (Cy5), MMP-2 substrate peptide, and a dark quencher (BHQ-3); ( B ) Schematic diagram of the MMP-2-PLGA-PEI nanoparticles. The fluorescence signal was recovered only in the presence of MMP-2-positive cancer cells.
    Figure Legend Snippet: Schematic illustration of the matrix metalloproteinase-2 (MMP-2)-activated peptide sensor and MMP-2-activated poly(lactic-co-glycolic acid) with polyethylenimine (MMP-2-PLGA-PEI) nanoparticles. ( A ) Chemical structure of the MMP-2-activated peptide sensor. The sensor consisted of a near-infrared fluorescence dye (Cy5), MMP-2 substrate peptide, and a dark quencher (BHQ-3); ( B ) Schematic diagram of the MMP-2-PLGA-PEI nanoparticles. The fluorescence signal was recovered only in the presence of MMP-2-positive cancer cells.

    Techniques Used: Fluorescence

    6) Product Images from "Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes"

    Article Title: Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes

    Journal: Frontiers in Chemistry

    doi: 10.3389/fchem.2019.00051

    bPEI-DNA complex immobilization on PAA brushes at pH 7.2. Complex formation with DNA plasmid encoding for enhanced green fluorescent protein (eGFP) and luciferase (LUC) and branched polyethylenimine (bPEI) at a N/P ratio of 20 complexes with an overall positive charge (6 mV). These positively charged bPEI-DNA complexes can interact with negatively charged, swollen PAA-RGD brushes (pH 7.2) on the substrate to transfect NIH/3T3 fibroblasts cultured on the substrate.
    Figure Legend Snippet: bPEI-DNA complex immobilization on PAA brushes at pH 7.2. Complex formation with DNA plasmid encoding for enhanced green fluorescent protein (eGFP) and luciferase (LUC) and branched polyethylenimine (bPEI) at a N/P ratio of 20 complexes with an overall positive charge (6 mV). These positively charged bPEI-DNA complexes can interact with negatively charged, swollen PAA-RGD brushes (pH 7.2) on the substrate to transfect NIH/3T3 fibroblasts cultured on the substrate.

    Techniques Used: Plasmid Preparation, Luciferase, Cell Culture

    7) Product Images from "Morphology-Variable Aggregates Prepared from Cholesterol-Containing Amphiphilic Glycopolymers: Their Protein Recognition/Adsorption and Drug Delivery Applications"

    Article Title: Morphology-Variable Aggregates Prepared from Cholesterol-Containing Amphiphilic Glycopolymers: Their Protein Recognition/Adsorption and Drug Delivery Applications

    Journal: Nanomaterials

    doi: 10.3390/nano8030136

    BSA adsorption assay for the PMAgala 18 and PMAgala 18 - b -PMAChol aggregates under 37 °C in aqueous solution for an incubation of 3 h ( a ) and 5 h ( b ). Poly(ethylene glycol) with a molecular weight of 5000 Da (PEG-5K) and branched polyethylenimine with molecular weight of 25,000 Da (PEI-25K) were employed as the negative and positive controls, respectively. BSA protein mass concentration was preset to be 0.5 mg/mL.
    Figure Legend Snippet: BSA adsorption assay for the PMAgala 18 and PMAgala 18 - b -PMAChol aggregates under 37 °C in aqueous solution for an incubation of 3 h ( a ) and 5 h ( b ). Poly(ethylene glycol) with a molecular weight of 5000 Da (PEG-5K) and branched polyethylenimine with molecular weight of 25,000 Da (PEI-25K) were employed as the negative and positive controls, respectively. BSA protein mass concentration was preset to be 0.5 mg/mL.

    Techniques Used: Adsorption, Incubation, Molecular Weight, Concentration Assay

    8) Product Images from "Biophysical Characterization of Copolymer-Protected Gene Vectors (COPROGs)"

    Article Title: Biophysical Characterization of Copolymer-Protected Gene Vectors (COPROGs)

    Journal: Biomacromolecules

    doi: 10.1021/bm1002569

    Schematic drawing of the self-assembly of copolymer protected gene vectors (COPROGs). Plasmid DNA are mixed with 25kDa branched polyethylenimine (N/P = 8) to form a bPEI/DNA precomplex. After incubation, the positively overcharged precomplex is mixed
    Figure Legend Snippet: Schematic drawing of the self-assembly of copolymer protected gene vectors (COPROGs). Plasmid DNA are mixed with 25kDa branched polyethylenimine (N/P = 8) to form a bPEI/DNA precomplex. After incubation, the positively overcharged precomplex is mixed

    Techniques Used: Plasmid Preparation, Incubation

    9) Product Images from "Development of PVDF Membrane Nanocomposites via Various Functionalization Approaches for Environmental Applications"

    Article Title: Development of PVDF Membrane Nanocomposites via Various Functionalization Approaches for Environmental Applications

    Journal: Polymers

    doi: 10.3390/polym8020032

    Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) of PVDF-PAA membrane ( black line), PVDF-PAA-PEI membrane ( red line), and polyethylenimine (PEI) solution ( green line).
    Figure Legend Snippet: Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) of PVDF-PAA membrane ( black line), PVDF-PAA-PEI membrane ( red line), and polyethylenimine (PEI) solution ( green line).

    Techniques Used: Spectroscopy

    10) Product Images from "Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator"

    Article Title: Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator

    Journal: eLife

    doi: 10.7554/eLife.10482

    A polymer cushion improves clamp speed under a peeled oocyte. The speed of the voltage clamp on the bottom side of an oocyte is observed by monitoring the fluorescence of di-8-ANEPPS in the oocyte membrane. When a peeled oocyte is placed directly on a clean glass coverslip, it seals against the glass and creates a slow voltage clamp (red trace). However, coating the coverslip with a water-soluble polymer (polyethylenimine) decreases access resistance under the oocyte and thus improves clamp speed in this region (blue trace). DOI: http://dx.doi.org/10.7554/eLife.10482.005
    Figure Legend Snippet: A polymer cushion improves clamp speed under a peeled oocyte. The speed of the voltage clamp on the bottom side of an oocyte is observed by monitoring the fluorescence of di-8-ANEPPS in the oocyte membrane. When a peeled oocyte is placed directly on a clean glass coverslip, it seals against the glass and creates a slow voltage clamp (red trace). However, coating the coverslip with a water-soluble polymer (polyethylenimine) decreases access resistance under the oocyte and thus improves clamp speed in this region (blue trace). DOI: http://dx.doi.org/10.7554/eLife.10482.005

    Techniques Used: Fluorescence

    11) Product Images from "Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites"

    Article Title: Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules22010091

    SDS-PAGE analysis of OC-CALB biocatalysts coating with polyethylenimine and dextran sulfate after desorption of the lipase without inactivation process. The biocatalyst was submitted to the processes described in the Experimental section. Gel shows the enzyme that remains bound to the support after desorption. Unless otherwise indicated, all the desorption experiments were performed at 25 °C. Lane 1: molecular weight marker. Lane 2: OC-CALB derivative. Lane 3: OC-CALB-PEI-DS derivative. Lane 4: after desorption with 2% SDS (pH 7). Lane 5: desorption with 2% SDS in 2 M sodium phosphate (pH 7). Lane 6: desorption with 2% SDS in 2 M sodium phosphate (pH 3). Lane 7: desorption with 2% SDS in 2 M sodium phosphate (pH 3) at 45 °C.
    Figure Legend Snippet: SDS-PAGE analysis of OC-CALB biocatalysts coating with polyethylenimine and dextran sulfate after desorption of the lipase without inactivation process. The biocatalyst was submitted to the processes described in the Experimental section. Gel shows the enzyme that remains bound to the support after desorption. Unless otherwise indicated, all the desorption experiments were performed at 25 °C. Lane 1: molecular weight marker. Lane 2: OC-CALB derivative. Lane 3: OC-CALB-PEI-DS derivative. Lane 4: after desorption with 2% SDS (pH 7). Lane 5: desorption with 2% SDS in 2 M sodium phosphate (pH 7). Lane 6: desorption with 2% SDS in 2 M sodium phosphate (pH 3). Lane 7: desorption with 2% SDS in 2 M sodium phosphate (pH 3) at 45 °C.

    Techniques Used: SDS Page, Molecular Weight, Marker

    12) Product Images from "Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy"

    Article Title: Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S108039

    Illustration of Gd-CDs/AFn (DOX)/FA nanocomposite preparation. Abbreviations: AFn, apoferritin; CDs, carbon dots; DEG, diethylene glycol; DOX, doxorubicin; EDC, N-(3-dimethylaminopropyl-N′-ethylcarbodiimide); FA, folic acid; Gd, gadolinium; NHS, N-hydroxysuccinimide; PEI, polyethylenimine.
    Figure Legend Snippet: Illustration of Gd-CDs/AFn (DOX)/FA nanocomposite preparation. Abbreviations: AFn, apoferritin; CDs, carbon dots; DEG, diethylene glycol; DOX, doxorubicin; EDC, N-(3-dimethylaminopropyl-N′-ethylcarbodiimide); FA, folic acid; Gd, gadolinium; NHS, N-hydroxysuccinimide; PEI, polyethylenimine.

    Techniques Used:

    13) Product Images from "Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation"

    Article Title: Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20246307

    Cellular implications of tyrosine to phenylalanine mutation of EYA3 phosphotyrosine sites. ( a ) Endogenous EYA3 is tyrosine phosphorylated in HEK293T. Cells were transiently transfected with empty vector (pCiNeo), vector expressing c-Src Y527F (pSLX-c-Src Y527F) or treated only with the transfection reagent polyethylenimine (PEI). In lysates, EYA3, Src, and loading control (GAPDH) were detected by a Western blot. Endogenous EYA3 was immunoprecipitated and pY and EYA3 were detected by a Western blot. Two independent experiments were performed. ( b ) HEK293T cells, which overexpressed c-Src Y527F, were treated with benzbromarone (BB) versus dimethyl sulfoxide (DMSO, solvent for BB) to prove endogenous EYA3′s autodephosphorylation capacity. Src overexpression was detected by a Western blot, as were EYA3 and the loading control GAPDH. Endogenous EYA3 was immunoprecipitated and anti-pY and anti-EYA3 antibodies were used in a Western blot for the detection of tyrosine phosphorylated EYA3. Results show that EYA3 tyrosine phosphorylation (indicated by an arrow) is much more intense when BB is added in the cell culture media. Two independent experiments were performed. ( c ) Protein tyrosine phosphatase activity of two Y→F mutants of EYA3 tested using the synthetic substrate para-nitrophenylphosphate (pNPP). Myc-tagged EYA3 WT, D309N, WT T2, and WT T9 were transiently expressed in HEK293T cells. After immunoprecipitation (using anti-c-Myc antibody, 9E10), proteins were subjected to an in vitro PTP assay using pNPP as a substrate. The EYA3 WT T2 protein retained 90% of its PTP activity, whereas EYA3 WT T9 retained only 60%. Three independent experiments were conducted, with each having three technical replicates per sample ( n = 9). For each sample, the absorbance at 405 nm (A 405 nm ) was measured. The results shown in the graph represent the mean ± SEM of the values obtained after normalization to the A 405 nm of the EYA3 WT sample. Statistical analysis was made by applying unpaired Student’s t-test (two-tailed) (n.s. - not significant, * p
    Figure Legend Snippet: Cellular implications of tyrosine to phenylalanine mutation of EYA3 phosphotyrosine sites. ( a ) Endogenous EYA3 is tyrosine phosphorylated in HEK293T. Cells were transiently transfected with empty vector (pCiNeo), vector expressing c-Src Y527F (pSLX-c-Src Y527F) or treated only with the transfection reagent polyethylenimine (PEI). In lysates, EYA3, Src, and loading control (GAPDH) were detected by a Western blot. Endogenous EYA3 was immunoprecipitated and pY and EYA3 were detected by a Western blot. Two independent experiments were performed. ( b ) HEK293T cells, which overexpressed c-Src Y527F, were treated with benzbromarone (BB) versus dimethyl sulfoxide (DMSO, solvent for BB) to prove endogenous EYA3′s autodephosphorylation capacity. Src overexpression was detected by a Western blot, as were EYA3 and the loading control GAPDH. Endogenous EYA3 was immunoprecipitated and anti-pY and anti-EYA3 antibodies were used in a Western blot for the detection of tyrosine phosphorylated EYA3. Results show that EYA3 tyrosine phosphorylation (indicated by an arrow) is much more intense when BB is added in the cell culture media. Two independent experiments were performed. ( c ) Protein tyrosine phosphatase activity of two Y→F mutants of EYA3 tested using the synthetic substrate para-nitrophenylphosphate (pNPP). Myc-tagged EYA3 WT, D309N, WT T2, and WT T9 were transiently expressed in HEK293T cells. After immunoprecipitation (using anti-c-Myc antibody, 9E10), proteins were subjected to an in vitro PTP assay using pNPP as a substrate. The EYA3 WT T2 protein retained 90% of its PTP activity, whereas EYA3 WT T9 retained only 60%. Three independent experiments were conducted, with each having three technical replicates per sample ( n = 9). For each sample, the absorbance at 405 nm (A 405 nm ) was measured. The results shown in the graph represent the mean ± SEM of the values obtained after normalization to the A 405 nm of the EYA3 WT sample. Statistical analysis was made by applying unpaired Student’s t-test (two-tailed) (n.s. - not significant, * p

    Techniques Used: Mutagenesis, Transfection, Plasmid Preparation, Expressing, Western Blot, Immunoprecipitation, Over Expression, Cell Culture, Activity Assay, In Vitro, Two Tailed Test

    14) Product Images from "Single-Step Metal-Free Grafting of Cationic Polymer Brushes on Fluorescent Nanodiamonds"

    Article Title: Single-Step Metal-Free Grafting of Cationic Polymer Brushes on Fluorescent Nanodiamonds

    Journal: Materials

    doi: 10.3390/ma11081479

    Cell viability testing of HeLa cells treated with FND-bare, FND-HPGTMA, and FND-PEI, and PEI for 24 h using CCK-8. Values are means ± standard deviations of three measurements. PEI: Polyethylenimine.
    Figure Legend Snippet: Cell viability testing of HeLa cells treated with FND-bare, FND-HPGTMA, and FND-PEI, and PEI for 24 h using CCK-8. Values are means ± standard deviations of three measurements. PEI: Polyethylenimine.

    Techniques Used: CCK-8 Assay

    15) Product Images from "(?-NaYbF4:Tm3+)/CaF2 Core/Shell Nanoparticles with Efficient Near-Infrared to Near-Infrared Upconversion for High-Contrast Deep Tissue Bioimaging"

    Article Title: (?-NaYbF4:Tm3+)/CaF2 Core/Shell Nanoparticles with Efficient Near-Infrared to Near-Infrared Upconversion for High-Contrast Deep Tissue Bioimaging

    Journal: ACS nano

    doi: 10.1021/nn302972r

    Polyethyleneimine-coated NIR in -NIR out α-(NaYbF 4 :0.5%Tm 3+ )/CaF 2 core/shell nanoparticles for imaging a synthetic periosteal mesh implanted around a rat femur. a) UCNPs were loaded on a 7-mm wide sulfated polymer mesh and wrapped around the mid-shaft
    Figure Legend Snippet: Polyethyleneimine-coated NIR in -NIR out α-(NaYbF 4 :0.5%Tm 3+ )/CaF 2 core/shell nanoparticles for imaging a synthetic periosteal mesh implanted around a rat femur. a) UCNPs were loaded on a 7-mm wide sulfated polymer mesh and wrapped around the mid-shaft

    Techniques Used: Imaging

    16) Product Images from "Rational design, fabrication, characterization and in vitro testing of biodegradable microparticles that generate targeted and sustained transgene expression in HepG2 liver cells"

    Article Title: Rational design, fabrication, characterization and in vitro testing of biodegradable microparticles that generate targeted and sustained transgene expression in HepG2 liver cells

    Journal: Journal of drug targeting

    doi: 10.3109/1061186X.2010.504263

    Chemical structure of (a) linear polyethylenimine (b) branched polyethylenimine and (c) poly (lactide-co-glycolide).
    Figure Legend Snippet: Chemical structure of (a) linear polyethylenimine (b) branched polyethylenimine and (c) poly (lactide-co-glycolide).

    Techniques Used:

    17) Product Images from "Effects of Decomplexation Rates on Ternary Gene Complex Transfection with α-Poly(l-Lysine) or ε-Poly(l-Lysine) as a Decomplexation Controller in An Easy-To-Transfect Cell or A Hard-To-Transfect Cell"

    Article Title: Effects of Decomplexation Rates on Ternary Gene Complex Transfection with α-Poly(l-Lysine) or ε-Poly(l-Lysine) as a Decomplexation Controller in An Easy-To-Transfect Cell or A Hard-To-Transfect Cell

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics12060490

    Heparin-induced decomplexation of ( A ) poly( l -lysine) (PL)/pDNA nanocomplexes (NCs) and ( B ) branched polyethylenimine (bPEI)-PL/pDNA NCs. After the polyplexes were exposed to heparin-containing aqueous NaCl (150 mM) at 37 °C for 30 min, the polyplexes were electrophoresed in 0.8% agarose gel. Red- or green-colored arrows indicate exposed or released pDNA, respectively.
    Figure Legend Snippet: Heparin-induced decomplexation of ( A ) poly( l -lysine) (PL)/pDNA nanocomplexes (NCs) and ( B ) branched polyethylenimine (bPEI)-PL/pDNA NCs. After the polyplexes were exposed to heparin-containing aqueous NaCl (150 mM) at 37 °C for 30 min, the polyplexes were electrophoresed in 0.8% agarose gel. Red- or green-colored arrows indicate exposed or released pDNA, respectively.

    Techniques Used: Agarose Gel Electrophoresis

    18) Product Images from "Morphology-Variable Aggregates Prepared from Cholesterol-Containing Amphiphilic Glycopolymers: Their Protein Recognition/Adsorption and Drug Delivery Applications"

    Article Title: Morphology-Variable Aggregates Prepared from Cholesterol-Containing Amphiphilic Glycopolymers: Their Protein Recognition/Adsorption and Drug Delivery Applications

    Journal: Nanomaterials

    doi: 10.3390/nano8030136

    BSA adsorption assay for the PMAgala 18 and PMAgala 18 - b -PMAChol aggregates under 37 °C in aqueous solution for an incubation of 3 h ( a ) and 5 h ( b ). Poly(ethylene glycol) with a molecular weight of 5000 Da (PEG-5K) and branched polyethylenimine with molecular weight of 25,000 Da (PEI-25K) were employed as the negative and positive controls, respectively. BSA protein mass concentration was preset to be 0.5 mg/mL.
    Figure Legend Snippet: BSA adsorption assay for the PMAgala 18 and PMAgala 18 - b -PMAChol aggregates under 37 °C in aqueous solution for an incubation of 3 h ( a ) and 5 h ( b ). Poly(ethylene glycol) with a molecular weight of 5000 Da (PEG-5K) and branched polyethylenimine with molecular weight of 25,000 Da (PEI-25K) were employed as the negative and positive controls, respectively. BSA protein mass concentration was preset to be 0.5 mg/mL.

    Techniques Used: Adsorption, Incubation, Molecular Weight, Concentration Assay

    19) Product Images from "Visualization of MMP-2 Activity Using Dual-Probe Nanoparticles to Detect Potential Metastatic Cancer Cells"

    Article Title: Visualization of MMP-2 Activity Using Dual-Probe Nanoparticles to Detect Potential Metastatic Cancer Cells

    Journal: Nanomaterials

    doi: 10.3390/nano8020119

    Schematic illustration of the matrix metalloproteinase-2 (MMP-2)-activated peptide sensor and MMP-2-activated poly(lactic-co-glycolic acid) with polyethylenimine (MMP-2-PLGA-PEI) nanoparticles. ( A ) Chemical structure of the MMP-2-activated peptide sensor. The sensor consisted of a near-infrared fluorescence dye (Cy5), MMP-2 substrate peptide, and a dark quencher (BHQ-3); ( B ) Schematic diagram of the MMP-2-PLGA-PEI nanoparticles. The fluorescence signal was recovered only in the presence of MMP-2-positive cancer cells.
    Figure Legend Snippet: Schematic illustration of the matrix metalloproteinase-2 (MMP-2)-activated peptide sensor and MMP-2-activated poly(lactic-co-glycolic acid) with polyethylenimine (MMP-2-PLGA-PEI) nanoparticles. ( A ) Chemical structure of the MMP-2-activated peptide sensor. The sensor consisted of a near-infrared fluorescence dye (Cy5), MMP-2 substrate peptide, and a dark quencher (BHQ-3); ( B ) Schematic diagram of the MMP-2-PLGA-PEI nanoparticles. The fluorescence signal was recovered only in the presence of MMP-2-positive cancer cells.

    Techniques Used: Fluorescence

    20) Product Images from "Injectable nanofibrous spongy microspheres for NR4A1 plasmid DNA transfection to reverse fibrotic degeneration and support disc regeneration"

    Article Title: Injectable nanofibrous spongy microspheres for NR4A1 plasmid DNA transfection to reverse fibrotic degeneration and support disc regeneration

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2017.03.029

    In vitro luciferase expression (A), cytotoxicity (B) and GFP gene transfection efficiency (C-G) (A) HP, LP, and PEI 25KD (polyethylenimine, molecular weight = 25 kDa) loaded with Luc pDNA at varying N/P ratios transfected into NP cells. HP achieved ∼10 times higher luciferase expression compared to LP at N/P ratios of 4 and 8. Higher N/P ratios were necessary for LP to obtain comparable transfection efficiency to HP. (B) For N/P ratios of 2-32, the toxicity level of HP was comparable to LP and almost negligible. No increased cytotoxicity due to the hyperbranched structure was observed. In the PEI 25KD group, significantly lower cell viability was observed for N/P ratios of 8-32, which indicated significant cytotoxicity of this polymer. (C-G) Representative fluorescence microscopy images of transduced cells at 48 h post-transfection: (C) naked pDNA, (D) PEI 25K-pDNA, (E) LP-pDNA, and (F) HP-pDNA. (G) The GFP positive ratios of nucleus pulposus cells after gene transfection determined by flow cytometry. Error bars in the graph represent SEM, n = 4, * P
    Figure Legend Snippet: In vitro luciferase expression (A), cytotoxicity (B) and GFP gene transfection efficiency (C-G) (A) HP, LP, and PEI 25KD (polyethylenimine, molecular weight = 25 kDa) loaded with Luc pDNA at varying N/P ratios transfected into NP cells. HP achieved ∼10 times higher luciferase expression compared to LP at N/P ratios of 4 and 8. Higher N/P ratios were necessary for LP to obtain comparable transfection efficiency to HP. (B) For N/P ratios of 2-32, the toxicity level of HP was comparable to LP and almost negligible. No increased cytotoxicity due to the hyperbranched structure was observed. In the PEI 25KD group, significantly lower cell viability was observed for N/P ratios of 8-32, which indicated significant cytotoxicity of this polymer. (C-G) Representative fluorescence microscopy images of transduced cells at 48 h post-transfection: (C) naked pDNA, (D) PEI 25K-pDNA, (E) LP-pDNA, and (F) HP-pDNA. (G) The GFP positive ratios of nucleus pulposus cells after gene transfection determined by flow cytometry. Error bars in the graph represent SEM, n = 4, * P

    Techniques Used: In Vitro, Luciferase, Expressing, Transfection, Molecular Weight, Fluorescence, Microscopy, Flow Cytometry, Cytometry

    21) Product Images from "Phosphoramidate End-labeling of Inorganic Polyphosphates: Facile Manipulation of Polyphosphate for Investigating and Modulating its Biological Activities †"

    Article Title: Phosphoramidate End-labeling of Inorganic Polyphosphates: Facile Manipulation of Polyphosphate for Investigating and Modulating its Biological Activities †

    Journal: Biochemistry

    doi: 10.1021/bi1014437

    Immobilized and derivatized polyP retains procoagulant activity. (A) PolyP HMW was immobilized via EDAC onto polyethylenimine-coated polystyrene coagulometer cuvettes. Clotting was then initiated by incubating human plasma in the wells for 3 min at 37°C, after which CaCl 2 was added and the time to clot formation recorded. Control cuvettes included those untreated with polyethylenimine, EDAC and/or polyP, as indicated. (B) Clotting assays were conducted as in panel A except that untreated cuvettes were employed and 20 μM polyP HMW -spermidine in solution was preincubated with plasma for 3 min at 37°C, after which CaCl 2 was added and the time to clot formation recorded. Controls included polyP HMW that had been reacted without EDAC and/or spermidine, and also wells that received plasma but no polyP, as indicated. Data are mean ± S.E.M. ( n =3).
    Figure Legend Snippet: Immobilized and derivatized polyP retains procoagulant activity. (A) PolyP HMW was immobilized via EDAC onto polyethylenimine-coated polystyrene coagulometer cuvettes. Clotting was then initiated by incubating human plasma in the wells for 3 min at 37°C, after which CaCl 2 was added and the time to clot formation recorded. Control cuvettes included those untreated with polyethylenimine, EDAC and/or polyP, as indicated. (B) Clotting assays were conducted as in panel A except that untreated cuvettes were employed and 20 μM polyP HMW -spermidine in solution was preincubated with plasma for 3 min at 37°C, after which CaCl 2 was added and the time to clot formation recorded. Controls included polyP HMW that had been reacted without EDAC and/or spermidine, and also wells that received plasma but no polyP, as indicated. Data are mean ± S.E.M. ( n =3).

    Techniques Used: Activity Assay, Coagulation

    22) Product Images from "Single-Step Metal-Free Grafting of Cationic Polymer Brushes on Fluorescent Nanodiamonds"

    Article Title: Single-Step Metal-Free Grafting of Cationic Polymer Brushes on Fluorescent Nanodiamonds

    Journal: Materials

    doi: 10.3390/ma11081479

    Cell viability testing of HeLa cells treated with FND-bare, FND-HPGTMA, and FND-PEI, and PEI for 24 h using CCK-8. Values are means ± standard deviations of three measurements. PEI: Polyethylenimine.
    Figure Legend Snippet: Cell viability testing of HeLa cells treated with FND-bare, FND-HPGTMA, and FND-PEI, and PEI for 24 h using CCK-8. Values are means ± standard deviations of three measurements. PEI: Polyethylenimine.

    Techniques Used: CCK-8 Assay

    23) Product Images from "RNA-based, transient modulation of gene expression in human haematopoietic stem and progenitor cells"

    Article Title: RNA-based, transient modulation of gene expression in human haematopoietic stem and progenitor cells

    Journal: Scientific Reports

    doi: 10.1038/srep17184

    Chemical transfection of HSPCs with functional siRNAs shows no target protein knockdown but electroporation does. CD133 + HSPCs or indicated cell lines (Weri-RB1, K562) were transfected with siRNAs targeting CD133 ( a,c ), CD45 ( b,d ) or a non-targeting control siRNA (ctrl). Displayed is the median fluorescence intensity (MFI) of the respective surface marker as percentage relative to the control siRNA ( a,b ) Relative protein expression at the time of maximum knockdown (72 h after transfection, ( # ) 24 h after transfection; n = 2–10). ( c,d ) Kinetics of relative protein expression at the indicated time points after transfection. Open circles (О) represent cells transfected with non-targeting control siRNA (Neg-siRNA), closed triangles (▲) represent CD133- and CD45-siRNA in ( c,d ), respectively (mean of n = 3 at day 1–3, mean of n = 2 at day 6 and 8). LF: Lipofectamine 2000, RM: Lipofectamine RNAiMAX, HP: HiPerfect, Elpo: Electroporation, PEI: Polyethylenimine 2 K. Error bars represent SD. ***P ≤ 0.001, ****P ≤ 0.0001, ns = not significant, one-way ANOVA.
    Figure Legend Snippet: Chemical transfection of HSPCs with functional siRNAs shows no target protein knockdown but electroporation does. CD133 + HSPCs or indicated cell lines (Weri-RB1, K562) were transfected with siRNAs targeting CD133 ( a,c ), CD45 ( b,d ) or a non-targeting control siRNA (ctrl). Displayed is the median fluorescence intensity (MFI) of the respective surface marker as percentage relative to the control siRNA ( a,b ) Relative protein expression at the time of maximum knockdown (72 h after transfection, ( # ) 24 h after transfection; n = 2–10). ( c,d ) Kinetics of relative protein expression at the indicated time points after transfection. Open circles (О) represent cells transfected with non-targeting control siRNA (Neg-siRNA), closed triangles (▲) represent CD133- and CD45-siRNA in ( c,d ), respectively (mean of n = 3 at day 1–3, mean of n = 2 at day 6 and 8). LF: Lipofectamine 2000, RM: Lipofectamine RNAiMAX, HP: HiPerfect, Elpo: Electroporation, PEI: Polyethylenimine 2 K. Error bars represent SD. ***P ≤ 0.001, ****P ≤ 0.0001, ns = not significant, one-way ANOVA.

    Techniques Used: Transfection, Functional Assay, Electroporation, Fluorescence, Marker, Expressing

    Transfection of fluorescent siRNA into CD133 + HSPCs with liposomal reagents and cationic polymers. Cells were analysed 19 to 24 h after transfection with different reagents and either a non-labelled, non-targeting negative control (siRNA-Neg) or a fluorescently labelled, non-targeting siRNA (siRNA-AF488). Cells were washed, stained with CD133-PE, and analysed via flow cytometry. ( a ) Representative dot plots after transfection with Lipofectamine 2000 (LF) and HiPerFect (HP), gated on viable cells. ( b ) Percentage of total AF488 + cells after optimisation of transfection conditions. Each dot represents one independent experiment (n = 2–8). RM: Lipofectamine RNAiMAX, DT: DOTAP, PEI: Polyethylenimine 2K, EG: ExGen 500.
    Figure Legend Snippet: Transfection of fluorescent siRNA into CD133 + HSPCs with liposomal reagents and cationic polymers. Cells were analysed 19 to 24 h after transfection with different reagents and either a non-labelled, non-targeting negative control (siRNA-Neg) or a fluorescently labelled, non-targeting siRNA (siRNA-AF488). Cells were washed, stained with CD133-PE, and analysed via flow cytometry. ( a ) Representative dot plots after transfection with Lipofectamine 2000 (LF) and HiPerFect (HP), gated on viable cells. ( b ) Percentage of total AF488 + cells after optimisation of transfection conditions. Each dot represents one independent experiment (n = 2–8). RM: Lipofectamine RNAiMAX, DT: DOTAP, PEI: Polyethylenimine 2K, EG: ExGen 500.

    Techniques Used: Transfection, Negative Control, Staining, Flow Cytometry, Cytometry

    24) Product Images from "Silica nanoparticle-based dual imaging colloidal hybrids: cancer cell imaging and biodistribution"

    Article Title: Silica nanoparticle-based dual imaging colloidal hybrids: cancer cell imaging and biodistribution

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S88311

    Synthesis of dual imaging silica nanoparticles. Abbreviations: Gd, gadolinium; NHS, N-hydroxysuccinate; PEG, poly(ethylene glycol); PEI, polyethylenimine; TPS, trimethoxy(3-[oxiran-2-ylmethoxy]propyl)silane.
    Figure Legend Snippet: Synthesis of dual imaging silica nanoparticles. Abbreviations: Gd, gadolinium; NHS, N-hydroxysuccinate; PEG, poly(ethylene glycol); PEI, polyethylenimine; TPS, trimethoxy(3-[oxiran-2-ylmethoxy]propyl)silane.

    Techniques Used: Imaging

    25) Product Images from "Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo"

    Article Title: Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2008.04.014

    Transfection mediated by linear polyethylenimine (25 KDa) (LPEI)-pDNA (1 µg) nanoparticle complexes and branched polyethylenimine (25 KDa) (BPEI)-pDNA nanoparticle complexes at various N/P ratios in (a) HeLa, (b) HEK, (c) COS7 and (d) HepG2. Luciferase
    Figure Legend Snippet: Transfection mediated by linear polyethylenimine (25 KDa) (LPEI)-pDNA (1 µg) nanoparticle complexes and branched polyethylenimine (25 KDa) (BPEI)-pDNA nanoparticle complexes at various N/P ratios in (a) HeLa, (b) HEK, (c) COS7 and (d) HepG2. Luciferase

    Techniques Used: Transfection, Luciferase

    Chemical structure of (a) linear and (b) branched polyethylenimine
    Figure Legend Snippet: Chemical structure of (a) linear and (b) branched polyethylenimine

    Techniques Used:

    26) Product Images from "Comparison of EGF with VEGF Non-Viral Gene Therapy for Cutaneous Wound Healing of Streptozotocin Diabetic Mice"

    Article Title: Comparison of EGF with VEGF Non-Viral Gene Therapy for Cutaneous Wound Healing of Streptozotocin Diabetic Mice

    Journal: Diabetes & Metabolism Journal

    doi: 10.4093/dmj.2011.35.3.226

    Production of minicircle-vascular endothelial growth factor (VEGF) and the comparison of efficiency to a typical form of plasmid (pβ-VEGF) containing of bacterial backbone using branched polyethylenimine (BPEI) as a gene carrier in human embryonic kidney (HEK) 293 cells. (A) p2øC31-β-VEGF 165 the expression cassette from pβ-VEGF 165 was excised by restriction enzymes and bluntly ligated between attB and attP site of p2øC31 vector, which contains phi-C31 integrase and I-SceI homing endonuclease. (B) Minicircle-VEGF 165 . (C) Minicircle-VEGF 165 showed 2 to 3 folds higher transfection efficiency in HEK 293 cells. 2×10 5 cells of HEK293 were treated for 4 hours with plasmid DNA (pβ-VEGF 165 or Minicircle-VEGF 165 ) complexed with branched polyethylenimine (BPEI, 25 kDa, N/P ratio 10:1). VEGF concentrations in the culture media were measured by ELISA. a P
    Figure Legend Snippet: Production of minicircle-vascular endothelial growth factor (VEGF) and the comparison of efficiency to a typical form of plasmid (pβ-VEGF) containing of bacterial backbone using branched polyethylenimine (BPEI) as a gene carrier in human embryonic kidney (HEK) 293 cells. (A) p2øC31-β-VEGF 165 the expression cassette from pβ-VEGF 165 was excised by restriction enzymes and bluntly ligated between attB and attP site of p2øC31 vector, which contains phi-C31 integrase and I-SceI homing endonuclease. (B) Minicircle-VEGF 165 . (C) Minicircle-VEGF 165 showed 2 to 3 folds higher transfection efficiency in HEK 293 cells. 2×10 5 cells of HEK293 were treated for 4 hours with plasmid DNA (pβ-VEGF 165 or Minicircle-VEGF 165 ) complexed with branched polyethylenimine (BPEI, 25 kDa, N/P ratio 10:1). VEGF concentrations in the culture media were measured by ELISA. a P

    Techniques Used: Plasmid Preparation, Expressing, Transfection, Enzyme-linked Immunosorbent Assay

    27) Product Images from "Pt-Free Counter Electrodes with Carbon Black and 3D Network Epoxy Polymer Composites"

    Article Title: Pt-Free Counter Electrodes with Carbon Black and 3D Network Epoxy Polymer Composites

    Journal: Scientific Reports

    doi: 10.1038/srep22987

    ( a ) Illustration of the procedure used to fabricate the SS counter electrode containing a composite of CB and the 3D network polymers, and the chemical structures of trimethylolpropane triglycidyl ether (epoxy monomer) and polyethylenimine (hardener). A two-step spray pyrolysis process using a low wt% CB, followed by a high wt% CB, was used to prepare a conductive corrosion protective layer (CCPL) and a catalytic layer (CL). The in-situ cross-linking network polymerization reaction occurred instantly as soon as the mixed solution was sprayed onto a hot SS substrate or a hot CCPL. Cross-sectional images ( b ) and top-view images ( c ) obtained using field emission scanning electron microscopy (FE-SEM). Shown are the various composites prepared using CB and the 3D network polymers. Ash-color (or white) nanoparticles and the dark matrix represent dispersed CB and the 3D network polymers, respectively.
    Figure Legend Snippet: ( a ) Illustration of the procedure used to fabricate the SS counter electrode containing a composite of CB and the 3D network polymers, and the chemical structures of trimethylolpropane triglycidyl ether (epoxy monomer) and polyethylenimine (hardener). A two-step spray pyrolysis process using a low wt% CB, followed by a high wt% CB, was used to prepare a conductive corrosion protective layer (CCPL) and a catalytic layer (CL). The in-situ cross-linking network polymerization reaction occurred instantly as soon as the mixed solution was sprayed onto a hot SS substrate or a hot CCPL. Cross-sectional images ( b ) and top-view images ( c ) obtained using field emission scanning electron microscopy (FE-SEM). Shown are the various composites prepared using CB and the 3D network polymers. Ash-color (or white) nanoparticles and the dark matrix represent dispersed CB and the 3D network polymers, respectively.

    Techniques Used: In Situ, Electron Microscopy

    28) Product Images from "Luminescent/magnetic PLGA-based hybrid nanocomposites: a smart nanocarrier system for targeted codelivery and dual-modality imaging in cancer theranostics"

    Article Title: Luminescent/magnetic PLGA-based hybrid nanocomposites: a smart nanocarrier system for targeted codelivery and dual-modality imaging in cancer theranostics

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S136766

    Cellular uptake and endosomal/lysosomal escape of nanocomposites by confocal laser scanning microscopy. Notes: ( A ) HeLa cells were treated with free DOX, DM-PLGA, and DM-PLGA/PPF for 6 h. ( B ) Endosomal/lysosomal escape of DM-PLGA/PPF nanoparticles incubated with HeLa cells at a DOX concentration of 5 μg/mL and 37°C for various time intervals. Abbreviations: DOX, doxorubicin; DAPI, 4,6-diamino-2-phenylindole; PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.
    Figure Legend Snippet: Cellular uptake and endosomal/lysosomal escape of nanocomposites by confocal laser scanning microscopy. Notes: ( A ) HeLa cells were treated with free DOX, DM-PLGA, and DM-PLGA/PPF for 6 h. ( B ) Endosomal/lysosomal escape of DM-PLGA/PPF nanoparticles incubated with HeLa cells at a DOX concentration of 5 μg/mL and 37°C for various time intervals. Abbreviations: DOX, doxorubicin; DAPI, 4,6-diamino-2-phenylindole; PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.

    Techniques Used: Confocal Laser Scanning Microscopy, Incubation, Concentration Assay

    In vitro hemolysis assay. Hemolytic activity of the LM-PLGA ( A ) and LM-PLGA/PPF ( B ) at various nanocomposite concentrations (50, 100, 200, 300, and 400 μg/mL, respectively), incubated with rat red blood cells at 37°C for 2 h. Saline and water were used as negative and positive controls, respectively. Insets on the top right are the enlarged UV-vis spectra and bottom-right insets show the photograph of red blood cells treated with LM-PLGA and LM-PLGA/PPF at various concentrations. ( C ) Optical microscopy images of the dispersion of erythrocytes treated with different nanoparticles. Saline was used as a control. Abbreviations: PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.
    Figure Legend Snippet: In vitro hemolysis assay. Hemolytic activity of the LM-PLGA ( A ) and LM-PLGA/PPF ( B ) at various nanocomposite concentrations (50, 100, 200, 300, and 400 μg/mL, respectively), incubated with rat red blood cells at 37°C for 2 h. Saline and water were used as negative and positive controls, respectively. Insets on the top right are the enlarged UV-vis spectra and bottom-right insets show the photograph of red blood cells treated with LM-PLGA and LM-PLGA/PPF at various concentrations. ( C ) Optical microscopy images of the dispersion of erythrocytes treated with different nanoparticles. Saline was used as a control. Abbreviations: PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.

    Techniques Used: In Vitro, Hemolysis Assay, Activity Assay, Incubation, Microscopy

    In vivo fluorescence imaging of EMT-6 tumor-bearing mice after intra-tumoral injection of saline (control) or L-PLGA/PPF nanoparticles at 0.5 and 2.5 h. Notes: The fluorescence signal excitation from CdSe/ZnS quantum dot incorporation into PLGA-based nanoparticles in tumor sites was strong, whereas no apparent fluorescent signal was observed in control mice. The color bar change from red to yellow indicates the gradual increase of fluorescence signal intensity. Tumors are marked by the red circle. Abbreviations: PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; PLGA, poly( d,l -lactic- co -glycolic acid).
    Figure Legend Snippet: In vivo fluorescence imaging of EMT-6 tumor-bearing mice after intra-tumoral injection of saline (control) or L-PLGA/PPF nanoparticles at 0.5 and 2.5 h. Notes: The fluorescence signal excitation from CdSe/ZnS quantum dot incorporation into PLGA-based nanoparticles in tumor sites was strong, whereas no apparent fluorescent signal was observed in control mice. The color bar change from red to yellow indicates the gradual increase of fluorescence signal intensity. Tumors are marked by the red circle. Abbreviations: PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; PLGA, poly( d,l -lactic- co -glycolic acid).

    Techniques Used: In Vivo, Fluorescence, Imaging, Mouse Assay, Injection

    Schematic illustration of the synthesis procedure of LDM-PLGA/PPF/VEGF shRNA nanocomposites for codelivery of DOX and VEGF shRNA in EMT-6 tumor models. Notes: ( A ) The preparation process of PEI-PEG-FA. ( B ) The construction of PLGA-based polymeric nanoparticles using a double emulsion solvent evaporation method. ( C ) The transport process of LDM-PLGA/PPF/VEGF shRNA nanocomposites and inhibition of tumor growth through cellular uptake via endocytosis, endosomal escape, intracellular VEGF shRNA, and DOX release. Abbreviations: DOX, doxorubicin; PLGA, poly( d,l -lactic- co -glycolic acid); PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; PPF, PEI-PEG-FA; shRNA, small hairpin RNA; VEGF, vascular endothelial growth factor; siRNA, small interfering RNA; QDs, quantum dots; EDC, N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride; NHS, N -hydroxysuccinimide.
    Figure Legend Snippet: Schematic illustration of the synthesis procedure of LDM-PLGA/PPF/VEGF shRNA nanocomposites for codelivery of DOX and VEGF shRNA in EMT-6 tumor models. Notes: ( A ) The preparation process of PEI-PEG-FA. ( B ) The construction of PLGA-based polymeric nanoparticles using a double emulsion solvent evaporation method. ( C ) The transport process of LDM-PLGA/PPF/VEGF shRNA nanocomposites and inhibition of tumor growth through cellular uptake via endocytosis, endosomal escape, intracellular VEGF shRNA, and DOX release. Abbreviations: DOX, doxorubicin; PLGA, poly( d,l -lactic- co -glycolic acid); PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; PPF, PEI-PEG-FA; shRNA, small hairpin RNA; VEGF, vascular endothelial growth factor; siRNA, small interfering RNA; QDs, quantum dots; EDC, N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride; NHS, N -hydroxysuccinimide.

    Techniques Used: shRNA, Evaporation, Inhibition, Small Interfering RNA

    UV-vis and photoluminescent characterization. Notes: ( A ) UV-vis absorption (a) and photoluminescence spectra (b) of free DOX and the as-prepared nanoparticles. ( B ) Photoluminescence spectra of CdSe/ZnS QDs (c) and LM-PLGA (d) (excitation wavelength 490 nm). ( C ) Photographs of LDM-PLGA/PPF and LD-PLGA/PPF with or without an applied magnetic field. ( D ) Elemental analysis of LDM-PLGA nanoparticles. Abbreviations: DOX, doxorubicin; PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; QDs, quantum dots.
    Figure Legend Snippet: UV-vis and photoluminescent characterization. Notes: ( A ) UV-vis absorption (a) and photoluminescence spectra (b) of free DOX and the as-prepared nanoparticles. ( B ) Photoluminescence spectra of CdSe/ZnS QDs (c) and LM-PLGA (d) (excitation wavelength 490 nm). ( C ) Photographs of LDM-PLGA/PPF and LD-PLGA/PPF with or without an applied magnetic field. ( D ) Elemental analysis of LDM-PLGA nanoparticles. Abbreviations: DOX, doxorubicin; PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; QDs, quantum dots.

    Techniques Used:

    T 2 -weighted MR images and quantitative signal intensity analysis of LDM-PLGA/PPF. Notes: ( A ) T 2 -weighted MR images and color T 2 -weighted MR images of HeLa cells treated with LDM-PLGA/PPF at nanoparticle concentrations of 80, 100, 150, 200, and 300 μg/mL (or Fe concentrations of 1.8, 2.3, 3.4, 4.6, and 6.8 μg/mL) for 12 h. The color bar change from red to blue indicates the gradual decrease of MR signal intensity. ( B ) Quantitative signal intensity analysis. ( C ) In vivo T 2 -weighted MR images of EMT-6 tumor-bearing mice before (left) and after (right) injection of LDM-PLGA/PPF. Tumors are marked by the red circle. Abbreviations: MR, magnetic resonance; PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.
    Figure Legend Snippet: T 2 -weighted MR images and quantitative signal intensity analysis of LDM-PLGA/PPF. Notes: ( A ) T 2 -weighted MR images and color T 2 -weighted MR images of HeLa cells treated with LDM-PLGA/PPF at nanoparticle concentrations of 80, 100, 150, 200, and 300 μg/mL (or Fe concentrations of 1.8, 2.3, 3.4, 4.6, and 6.8 μg/mL) for 12 h. The color bar change from red to blue indicates the gradual decrease of MR signal intensity. ( B ) Quantitative signal intensity analysis. ( C ) In vivo T 2 -weighted MR images of EMT-6 tumor-bearing mice before (left) and after (right) injection of LDM-PLGA/PPF. Tumors are marked by the red circle. Abbreviations: MR, magnetic resonance; PLGA, poly( d,l -lactic- co -glycolic acid); PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid.

    Techniques Used: In Vivo, Mouse Assay, Injection

    ( A ) Thermogravimetric analysis of LDM-PLGA and LDM-PLGA/PPF. ( B ) Agarose gel electrophoresis assay of LDM-PLGA/PPF/VEGF shRNA at various weight ratios of LDM-PLGA/PPF to VEGF shRNA. Abbreviations: PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; shRNA, small hairpin RNA; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: ( A ) Thermogravimetric analysis of LDM-PLGA and LDM-PLGA/PPF. ( B ) Agarose gel electrophoresis assay of LDM-PLGA/PPF/VEGF shRNA at various weight ratios of LDM-PLGA/PPF to VEGF shRNA. Abbreviations: PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; shRNA, small hairpin RNA; VEGF, vascular endothelial growth factor.

    Techniques Used: Agarose Gel Electrophoresis, shRNA

    Characterization of the as-synthesized nanoparticles. Notes: ( A ) Morphology: (a) Scanning electron microscopy images of the PLGA nanoparticles coloaded with QDs, DOX, and Fe 3 O 4 without modification (LDM-PLGA); (b) PLGA nanoparticles coloaded with QDs, DOX, and Fe 3 O 4 modified with PPF conjugate (LDM-PLGA/PPF); (c) PLGA nanoparticles coloaded with QDs, DOX, and Fe 3 O 4 modified with PPF conjugate and VEGF shRNA (LDM-PLGA/PPF/VEGF shRNA); (d) transmission electron microscopy images of LDM-PLGA. ( B ) The particle size distributions of (e) LDM-PLGA and (f) LDM-PLGA/PPF, determined by dynamic light scattering. ( C ) Zeta potentials of LDM-PLGA, LDM-PLGA/PPF, and LDM-PLGA/PPF/VEGF shRNA nanoparticle composites in PBS (pH 7.4) at room temperature (25°C). Abbreviations: DOX, doxorubicin; PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; QD, quantum dot; shRNA, small hairpin RNA; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Characterization of the as-synthesized nanoparticles. Notes: ( A ) Morphology: (a) Scanning electron microscopy images of the PLGA nanoparticles coloaded with QDs, DOX, and Fe 3 O 4 without modification (LDM-PLGA); (b) PLGA nanoparticles coloaded with QDs, DOX, and Fe 3 O 4 modified with PPF conjugate (LDM-PLGA/PPF); (c) PLGA nanoparticles coloaded with QDs, DOX, and Fe 3 O 4 modified with PPF conjugate and VEGF shRNA (LDM-PLGA/PPF/VEGF shRNA); (d) transmission electron microscopy images of LDM-PLGA. ( B ) The particle size distributions of (e) LDM-PLGA and (f) LDM-PLGA/PPF, determined by dynamic light scattering. ( C ) Zeta potentials of LDM-PLGA, LDM-PLGA/PPF, and LDM-PLGA/PPF/VEGF shRNA nanoparticle composites in PBS (pH 7.4) at room temperature (25°C). Abbreviations: DOX, doxorubicin; PPF, PEI-PEG-FA; PEI-PEG-FA, polyethyleneimine premodified with polyethylene glycol-folic acid; QD, quantum dot; shRNA, small hairpin RNA; VEGF, vascular endothelial growth factor.

    Techniques Used: Synthesized, Electron Microscopy, Modification, shRNA, Transmission Assay

    29) Product Images from "Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections"

    Article Title: Visual tracing of diffusion and biodistribution for amphiphilic cationic nanoparticles using photoacoustic imaging after ex vivo intravitreal injections

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S109986

    PA imaging of Cy7-labeled MPEG-PCL-g-PEI micelles in enucleated eyes. Notes: The red fluorescence signals might be blood vessels with nonspecific PA signals in the eyes. White and black circles designate the generated PA signal areas of Cy7-labeled amphiphilic micelles. Abbreviations: PA, photoacoustic; Cy, cyanine; MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; min, minutes; 2-2-2, MPEG 2000 Da-PCL 2000 Da-PEI 2000 Da; 2-6-2, MPEG 2000 Da-PCL 6000 Da-PEI 2000 Da; 5-2-2; MPEG 5000 Da-PCL 2000 Da-PEI 2000 Da.
    Figure Legend Snippet: PA imaging of Cy7-labeled MPEG-PCL-g-PEI micelles in enucleated eyes. Notes: The red fluorescence signals might be blood vessels with nonspecific PA signals in the eyes. White and black circles designate the generated PA signal areas of Cy7-labeled amphiphilic micelles. Abbreviations: PA, photoacoustic; Cy, cyanine; MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; min, minutes; 2-2-2, MPEG 2000 Da-PCL 2000 Da-PEI 2000 Da; 2-6-2, MPEG 2000 Da-PCL 6000 Da-PEI 2000 Da; 5-2-2; MPEG 5000 Da-PCL 2000 Da-PEI 2000 Da.

    Techniques Used: Imaging, Labeling, Fluorescence, Generated

    Confocal microscopy of the retina at 1 day ( A , E , and I ), 3 days ( B , F , and J ), 5 days ( C , G , and K ), and 7 days ( D , H , and L ) after intravitreal injection of Cy5-labeled micelles in rat. ( A – D ) Eyes injected with Cy5-labeled MPEG2000 Da-PCL2000 Da-g-PEI micelles. ( E – H ) Eyes injected with Cy5-labeled MPEG2000 Da-PCL6000 Da-g-PEI micelles. ( I – L ) A group of MPEG5000 Da-PCL2000 Da-g-PEI micelles. The fluorescent micelles diffused through the retinal layers and were concentrated in the RPE layer. Black arrows indicate this layer. Abbreviations: Cy, cyanine; MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; CH, choroid; RPE, retinal pigment epithelium.
    Figure Legend Snippet: Confocal microscopy of the retina at 1 day ( A , E , and I ), 3 days ( B , F , and J ), 5 days ( C , G , and K ), and 7 days ( D , H , and L ) after intravitreal injection of Cy5-labeled micelles in rat. ( A – D ) Eyes injected with Cy5-labeled MPEG2000 Da-PCL2000 Da-g-PEI micelles. ( E – H ) Eyes injected with Cy5-labeled MPEG2000 Da-PCL6000 Da-g-PEI micelles. ( I – L ) A group of MPEG5000 Da-PCL2000 Da-g-PEI micelles. The fluorescent micelles diffused through the retinal layers and were concentrated in the RPE layer. Black arrows indicate this layer. Abbreviations: Cy, cyanine; MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; CH, choroid; RPE, retinal pigment epithelium.

    Techniques Used: Confocal Microscopy, Injection, Labeling

    Cytotoxicity of MPEG-PCL-g-PEI micelles of various compositions on ARPE cells and cell viability were determined by MTT assay (n=6). Abbreviations: MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide; 2-2-2, MPEG 2000 Da-PCL 2000 Da-PEI 2000 Da; 2-6-2, MPEG 2000 Da-PCL 6000 Da-PEI 2000 Da; 5-2-2; MPEG 5000 Da-PCL 2000 Da-PEI 2000 Da.
    Figure Legend Snippet: Cytotoxicity of MPEG-PCL-g-PEI micelles of various compositions on ARPE cells and cell viability were determined by MTT assay (n=6). Abbreviations: MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; MTT, 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide; 2-2-2, MPEG 2000 Da-PCL 2000 Da-PEI 2000 Da; 2-6-2, MPEG 2000 Da-PCL 6000 Da-PEI 2000 Da; 5-2-2; MPEG 5000 Da-PCL 2000 Da-PEI 2000 Da.

    Techniques Used: MTT Assay

    Biodistribution of MPEG-PCL-g-PEI NPs after intravitreal injection at each time point ( A ) 1 day, ( B ) 3 days, ( C ) 5 days, and ( D ) 7 days. Note: The bar graphs were obtained from confocal microscopic images of whole eye cryosections. Abbreviations: MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; NPs, nanoparticles; RPE, retinal pigment epithelium; INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer; ONL, outer nuclear layer; NFL, nerve fiber layer; GCL, ganglion cell layer; IO/IS, inner segment of photoreceptor/outer segment of photoreceptor; 2-2-2, MPEG 2000 Da-PCL 2000 Da-PEI 2000 Da; 2-6-2, MPEG 2000 Da-PCL 6000 Da-PEI 2000 Da; 5-2-2; MPEG 5000 Da-PCL 2000 Da-PEI 2000 Da.
    Figure Legend Snippet: Biodistribution of MPEG-PCL-g-PEI NPs after intravitreal injection at each time point ( A ) 1 day, ( B ) 3 days, ( C ) 5 days, and ( D ) 7 days. Note: The bar graphs were obtained from confocal microscopic images of whole eye cryosections. Abbreviations: MPEG, monomethoxy poly(ethylene glycol); PCL, poly(ε-caprolactone); PEI, polyethylenimine; NPs, nanoparticles; RPE, retinal pigment epithelium; INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer; ONL, outer nuclear layer; NFL, nerve fiber layer; GCL, ganglion cell layer; IO/IS, inner segment of photoreceptor/outer segment of photoreceptor; 2-2-2, MPEG 2000 Da-PCL 2000 Da-PEI 2000 Da; 2-6-2, MPEG 2000 Da-PCL 6000 Da-PEI 2000 Da; 5-2-2; MPEG 5000 Da-PCL 2000 Da-PEI 2000 Da.

    Techniques Used: Injection

    30) Product Images from "Contact Killing of Gram-Positive and Gram-Negative Bacteria on PDMS Provided with Immobilized Hyperbranched Antibacterial Coatings"

    Article Title: Contact Killing of Gram-Positive and Gram-Negative Bacteria on PDMS Provided with Immobilized Hyperbranched Antibacterial Coatings

    Journal: Langmuir

    doi: 10.1021/acs.langmuir.9b02549

    Schematic Representation of the Modification of a Hyperbranched Polymer (HBP) with Polyethylenimine (PEI) That Is Subsequently Quaternized by Alkyl Halides (RX), with L = Caprolactam
    Figure Legend Snippet: Schematic Representation of the Modification of a Hyperbranched Polymer (HBP) with Polyethylenimine (PEI) That Is Subsequently Quaternized by Alkyl Halides (RX), with L = Caprolactam

    Techniques Used: Modification

    31) Product Images from "Lateral View Flow System for Studies of Cell Adhesion and Deformation under Flow Conditions"

    Article Title: Lateral View Flow System for Studies of Cell Adhesion and Deformation under Flow Conditions

    Journal: BioTechniques

    doi:

    Schematic of the experimental setup of the agarose-cast vessel and experimental setup An artificial vessel is formed in an agarose gel with the input port connected to the cell suspension reservoir and the output port connected to the syringe pump. The vessel surface is first treated with polyethylenimine and glutaraldehyde and then coated with fibronectin and E-selectin. (Top) Profile of the vessel and adherent cell observed in the objective. (Middle) Cross-section of the vessel with an adherent cell.
    Figure Legend Snippet: Schematic of the experimental setup of the agarose-cast vessel and experimental setup An artificial vessel is formed in an agarose gel with the input port connected to the cell suspension reservoir and the output port connected to the syringe pump. The vessel surface is first treated with polyethylenimine and glutaraldehyde and then coated with fibronectin and E-selectin. (Top) Profile of the vessel and adherent cell observed in the objective. (Middle) Cross-section of the vessel with an adherent cell.

    Techniques Used: Agarose Gel Electrophoresis

    32) Product Images from "Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA"

    Article Title: Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S82413

    Confocal laser scanning micrographs of YOYO-1 labeled pGL3 (green) on HEK293 cells at 1 hour ( A ) and 13 hours ( B ) post transfection. The cells were transfected by polymer/pGL3-YOYO-1 complexes (1 μg/mL pGL3), respectively. The nuclei were stained with DAPI (blue). Scale bar, 20 μm. Abbreviations: C-SHR, disulfide cross-linked stearylated polyarginine peptide modified with histidine; SHR, non-cross-linked stearylated polyarginine peptide; PEI, polyethylenimine; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride; pDNA, plasmid DNA.
    Figure Legend Snippet: Confocal laser scanning micrographs of YOYO-1 labeled pGL3 (green) on HEK293 cells at 1 hour ( A ) and 13 hours ( B ) post transfection. The cells were transfected by polymer/pGL3-YOYO-1 complexes (1 μg/mL pGL3), respectively. The nuclei were stained with DAPI (blue). Scale bar, 20 μm. Abbreviations: C-SHR, disulfide cross-linked stearylated polyarginine peptide modified with histidine; SHR, non-cross-linked stearylated polyarginine peptide; PEI, polyethylenimine; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride; pDNA, plasmid DNA.

    Techniques Used: Labeling, Transfection, Staining, Modification, Plasmid Preparation

    33) Product Images from "Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability"

    Article Title: Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S33670

    Chemical structures of kaempferol, PLGA, PEO-PPO-PEO, glycol chitosan, PLGA-PEI, and PAMAM dendrimer. Abbreviations: PEO-PPO-PEO, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide); PLGA, poly(DL-lactic acid-co-glycolic acid); PEI, polyethyleneimine; PAMAM, poly(amidoamine).
    Figure Legend Snippet: Chemical structures of kaempferol, PLGA, PEO-PPO-PEO, glycol chitosan, PLGA-PEI, and PAMAM dendrimer. Abbreviations: PEO-PPO-PEO, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide); PLGA, poly(DL-lactic acid-co-glycolic acid); PEI, polyethyleneimine; PAMAM, poly(amidoamine).

    Techniques Used:

    34) Product Images from "A role for ErbB signaling in the induction of reactive astrogliosis"

    Article Title: A role for ErbB signaling in the induction of reactive astrogliosis

    Journal: Cell Discovery

    doi: 10.1038/celldisc.2017.44

    Astrocyte-specific expression of dnEGFR blocked injury-induced ErbB activation in reactive astrocytes. ( a ) Representative images of TRE -YFP expression in astrocytes of Mlc1 -tTA mice at 1-month old. AAV- TRE -YFP was stereotaxically injected into indicated brain regions. Fixed brains collected 1 day later were sectioned and immunostained for Acsbg1 or GFAP to label astrocytes in the cerebral cortex or hippocampal hilus, respectively. White arrows, double-positive cells. ( b ) Efficient inhibition on activity of each ErbB receptor by dominant-negative mutant dnEGFR. Complementary DNA sequence of dnEGFR was amplified from genomic DNA of TRE -dnEGFR mice by PCR and cloned into pcDNA3.1-His/myc vector. pcDNA3.1-dnEGFR-myc was transfected into HEK293 cells by polyethylenimine (PEI) together with one of ErbB1–4 plasmids or an empty vector. Cells were lysed 24 h later and processed into WB with indicated antibodies. Any ErbB receptor when overexpressed in HEK293 cells would autophosphorylate itself independent of ligand stimulation. Shown are representative WB results, demonstrating the inhibition of dnEGFR on phosphorylation of each ErbB receptor. ( c ) Quantitative analyses of experiments in ( b ). *** P
    Figure Legend Snippet: Astrocyte-specific expression of dnEGFR blocked injury-induced ErbB activation in reactive astrocytes. ( a ) Representative images of TRE -YFP expression in astrocytes of Mlc1 -tTA mice at 1-month old. AAV- TRE -YFP was stereotaxically injected into indicated brain regions. Fixed brains collected 1 day later were sectioned and immunostained for Acsbg1 or GFAP to label astrocytes in the cerebral cortex or hippocampal hilus, respectively. White arrows, double-positive cells. ( b ) Efficient inhibition on activity of each ErbB receptor by dominant-negative mutant dnEGFR. Complementary DNA sequence of dnEGFR was amplified from genomic DNA of TRE -dnEGFR mice by PCR and cloned into pcDNA3.1-His/myc vector. pcDNA3.1-dnEGFR-myc was transfected into HEK293 cells by polyethylenimine (PEI) together with one of ErbB1–4 plasmids or an empty vector. Cells were lysed 24 h later and processed into WB with indicated antibodies. Any ErbB receptor when overexpressed in HEK293 cells would autophosphorylate itself independent of ligand stimulation. Shown are representative WB results, demonstrating the inhibition of dnEGFR on phosphorylation of each ErbB receptor. ( c ) Quantitative analyses of experiments in ( b ). *** P

    Techniques Used: Expressing, Activation Assay, Mouse Assay, Injection, Inhibition, Activity Assay, Dominant Negative Mutation, Sequencing, Amplification, Polymerase Chain Reaction, Clone Assay, Plasmid Preparation, Transfection, Western Blot

    35) Product Images from "Crosslinked Linear Polyethyleneimine Enhances Delivery of DNA to the Cytoplasm"

    Article Title: Crosslinked Linear Polyethyleneimine Enhances Delivery of DNA to the Cytoplasm

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2012.09.004

    Synthesis of crosslinked linear polyethylenimine using NHS/EDC chemistry with a diacid. For degradable crosslinks that break down by disulfide oxidation R contains a disulfide group.
    Figure Legend Snippet: Synthesis of crosslinked linear polyethylenimine using NHS/EDC chemistry with a diacid. For degradable crosslinks that break down by disulfide oxidation R contains a disulfide group.

    Techniques Used:

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    Millipore polyethyleneimine cellulose plate
    The RABV L protein generates the mRNA cap structure by an unconventional mechanism: ( a ) The recombinant RABV L protein (0.1 μg) was incubated with the indicated substrates under the standard conditions for the VSV L protein. Nuclease P 1 -digests of RNA products were analyzed by thin layer chromatography on a <t>polyethyleneimine-cellulose</t> plate (PEI-cellulose TLC), followed by autoradiography. The positions of the origin (ori.), guanosine nucleotides (GMP, GDP, GTP), and cap structures (GpppA, GppppA) are shown; ( b ) The recombinant RABV L protein (lane 2, 0.1 μg; lane 3, 0.2 μg) was incubated with [γ- 32 P]GTP. The reaction mixtures were analyzed by PEI-cellulose TLC followed by autoradiography. Lane 1 indicates no enzyme. Lane M shows the position of 32 P-labeled inorganic phosphate (P i ), which was generated by digestion of [γ- 32 P]GTP with alkaline phosphatase.
    Polyethyleneimine Cellulose Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine cellulose plate/product/Millipore
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    Millipore polyethyleneimine
    Scheme of synthesis of <t>polyethyleneimines</t> (PEI)-n.
    Polyethyleneimine, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore stearic acid modified branched polyethylenimine
    Scheme of synthesis of <t>polyethyleneimines</t> (PEI)-n.
    Stearic Acid Modified Branched Polyethylenimine, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The RABV L protein generates the mRNA cap structure by an unconventional mechanism: ( a ) The recombinant RABV L protein (0.1 μg) was incubated with the indicated substrates under the standard conditions for the VSV L protein. Nuclease P 1 -digests of RNA products were analyzed by thin layer chromatography on a polyethyleneimine-cellulose plate (PEI-cellulose TLC), followed by autoradiography. The positions of the origin (ori.), guanosine nucleotides (GMP, GDP, GTP), and cap structures (GpppA, GppppA) are shown; ( b ) The recombinant RABV L protein (lane 2, 0.1 μg; lane 3, 0.2 μg) was incubated with [γ- 32 P]GTP. The reaction mixtures were analyzed by PEI-cellulose TLC followed by autoradiography. Lane 1 indicates no enzyme. Lane M shows the position of 32 P-labeled inorganic phosphate (P i ), which was generated by digestion of [γ- 32 P]GTP with alkaline phosphatase.

    Journal: Viruses

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity

    doi: 10.3390/v8050144

    Figure Lengend Snippet: The RABV L protein generates the mRNA cap structure by an unconventional mechanism: ( a ) The recombinant RABV L protein (0.1 μg) was incubated with the indicated substrates under the standard conditions for the VSV L protein. Nuclease P 1 -digests of RNA products were analyzed by thin layer chromatography on a polyethyleneimine-cellulose plate (PEI-cellulose TLC), followed by autoradiography. The positions of the origin (ori.), guanosine nucleotides (GMP, GDP, GTP), and cap structures (GpppA, GppppA) are shown; ( b ) The recombinant RABV L protein (lane 2, 0.1 μg; lane 3, 0.2 μg) was incubated with [γ- 32 P]GTP. The reaction mixtures were analyzed by PEI-cellulose TLC followed by autoradiography. Lane 1 indicates no enzyme. Lane M shows the position of 32 P-labeled inorganic phosphate (P i ), which was generated by digestion of [γ- 32 P]GTP with alkaline phosphatase.

    Article Snippet: Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ].

    Techniques: Recombinant, Incubation, Thin Layer Chromatography, Autoradiography, Labeling, Generated

    Scheme of synthesis of polyethyleneimines (PEI)-n.

    Journal: Polymers

    Article Title: Influence of Cross-Linking Degree on Hydrodynamic Behavior and Stimulus-Sensitivity of Derivatives of Branched Polyethyleneimine

    doi: 10.3390/polym12051085

    Figure Lengend Snippet: Scheme of synthesis of polyethyleneimines (PEI)-n.

    Article Snippet: The acetylated derivative of branched polyethyleneimine (PEI-0), namely, poly-N-isobutyroylethyleneimine, was obtained by acylating polyethyleneimine with isobutyroyl chloride under the conditions of the Einhorn reaction (with methylene chloride solvent and triethylamine acceptor).

    Techniques: