Structured Review

Becton Dickinson polyethylenimine
Polyethylenimine, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethylenimine/product/Becton Dickinson
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
polyethylenimine - by Bioz Stars, 2020-08
91/100 stars

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Electroporation:

Article Title: Bre1a, a Histone H2B Ubiquitin Ligase, Regulates the Cell Cycle and Differentiation of Neural Precursor Cells
Article Snippet: .. After electroporation, the cells were plated onto 12 mm coverslips double coated with polyethylenimine and Matrigel basement matrix (0.6 mg/ml SFM; Becton Dickinson) and cultured in 100 μl of SFM containing FGF-2 and EGF for proliferation or in SFM containing 10% knock-out serum replacement (Invitrogen) for differentiation. ..

Transfection:

Article Title: Mutant p53 stimulates cell invasion through an interaction with Rad21 in human ovarian cancer cells
Article Snippet: .. Each transfection mixture was prepared by mixing the 1 μg DNA and 5 μg/ml polyethylenimine (BD Biosciences, San Joes, CA, USA) in serum-free Opti-MEM and incubating for 15 min at room temperature. .. The transfection mixture was slowly added to the cells, which were allowed to recover for an additional 24 h before experiment.

Construct:

Article Title: A Conserved Lipid-binding Loop in the Kindlin FERM F1 Domain Is Required for Kindlin-mediated ?IIb?3 Integrin Coactivation *
Article Snippet: .. Briefly, αIIbβ3-expressing CHO cells were co-transfected with the indicated GFP and DsRed expression constructs using Lipofectamine (Invitrogen) or polyethylenimine following the manufacturer's instructions, and 24 h later, the cells were suspended and incubated with ligand-mimetic anti-αIIbβ3 monoclonal antibody PAC1 IgM (BD Biosciences) or PAC1 Fab in the presence or absence of 10 m m EDTA. αIIbβ3 integrin expression was assessed in parallel by staining with D57 antibody. .. Bound PAC1 and D57 were detected using Alexa 647 fluorophore-conjugated goat anti-mouse IgM and anti-mouse IgG (Invitrogen), respectively.

Knock-Out:

Article Title: Bre1a, a Histone H2B Ubiquitin Ligase, Regulates the Cell Cycle and Differentiation of Neural Precursor Cells
Article Snippet: .. After electroporation, the cells were plated onto 12 mm coverslips double coated with polyethylenimine and Matrigel basement matrix (0.6 mg/ml SFM; Becton Dickinson) and cultured in 100 μl of SFM containing FGF-2 and EGF for proliferation or in SFM containing 10% knock-out serum replacement (Invitrogen) for differentiation. ..

Incubation:

Article Title: A Conserved Lipid-binding Loop in the Kindlin FERM F1 Domain Is Required for Kindlin-mediated ?IIb?3 Integrin Coactivation *
Article Snippet: .. Briefly, αIIbβ3-expressing CHO cells were co-transfected with the indicated GFP and DsRed expression constructs using Lipofectamine (Invitrogen) or polyethylenimine following the manufacturer's instructions, and 24 h later, the cells were suspended and incubated with ligand-mimetic anti-αIIbβ3 monoclonal antibody PAC1 IgM (BD Biosciences) or PAC1 Fab in the presence or absence of 10 m m EDTA. αIIbβ3 integrin expression was assessed in parallel by staining with D57 antibody. .. Bound PAC1 and D57 were detected using Alexa 647 fluorophore-conjugated goat anti-mouse IgM and anti-mouse IgG (Invitrogen), respectively.

Cell Culture:

Article Title: Bre1a, a Histone H2B Ubiquitin Ligase, Regulates the Cell Cycle and Differentiation of Neural Precursor Cells
Article Snippet: .. After electroporation, the cells were plated onto 12 mm coverslips double coated with polyethylenimine and Matrigel basement matrix (0.6 mg/ml SFM; Becton Dickinson) and cultured in 100 μl of SFM containing FGF-2 and EGF for proliferation or in SFM containing 10% knock-out serum replacement (Invitrogen) for differentiation. ..

Expressing:

Article Title: A Conserved Lipid-binding Loop in the Kindlin FERM F1 Domain Is Required for Kindlin-mediated ?IIb?3 Integrin Coactivation *
Article Snippet: .. Briefly, αIIbβ3-expressing CHO cells were co-transfected with the indicated GFP and DsRed expression constructs using Lipofectamine (Invitrogen) or polyethylenimine following the manufacturer's instructions, and 24 h later, the cells were suspended and incubated with ligand-mimetic anti-αIIbβ3 monoclonal antibody PAC1 IgM (BD Biosciences) or PAC1 Fab in the presence or absence of 10 m m EDTA. αIIbβ3 integrin expression was assessed in parallel by staining with D57 antibody. .. Bound PAC1 and D57 were detected using Alexa 647 fluorophore-conjugated goat anti-mouse IgM and anti-mouse IgG (Invitrogen), respectively.

Staining:

Article Title: A Conserved Lipid-binding Loop in the Kindlin FERM F1 Domain Is Required for Kindlin-mediated ?IIb?3 Integrin Coactivation *
Article Snippet: .. Briefly, αIIbβ3-expressing CHO cells were co-transfected with the indicated GFP and DsRed expression constructs using Lipofectamine (Invitrogen) or polyethylenimine following the manufacturer's instructions, and 24 h later, the cells were suspended and incubated with ligand-mimetic anti-αIIbβ3 monoclonal antibody PAC1 IgM (BD Biosciences) or PAC1 Fab in the presence or absence of 10 m m EDTA. αIIbβ3 integrin expression was assessed in parallel by staining with D57 antibody. .. Bound PAC1 and D57 were detected using Alexa 647 fluorophore-conjugated goat anti-mouse IgM and anti-mouse IgG (Invitrogen), respectively.

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    Becton Dickinson polyethylenimine mediated transfection
    Polyethylenimine Mediated Transfection, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson polyethyleneimine coated culture dishes
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    Becton Dickinson polyethylenimine coated
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