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Avantor polyethylenimine
Polyethylenimine, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethylenimine/product/Avantor
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
polyethylenimine - by Bioz Stars, 2020-08
92/100 stars

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Transfection:

Article Title: Crystallographic analysis of the laminin β2 short arm reveals how the LF domain is inserted into a regular array of LE domains
Article Snippet: .. The cells were transfected with the expression vector using polyethylenimine (PEI; VWR International) using a w /w ratio of DNA:PEI of 1:3. .. The filtered cell culture supernatant was adjusted to a final concentration of 20 mM Na-HEPES (pH 7.5) and loaded onto a 5-ml HisTrap Excel column (GE Healthcare) using an Äkta Purifier (GE Healthcare).

Article Title: C-Terminal Farnesylation of UCH-L1 Plays a Role in Transport of Epstein-Barr Virus Primary Oncoprotein LMP1 to Exosomes
Article Snippet: .. 293 cells were grown in 100-mm-diameter plates and transfected with 3 μg of plasmids with the use of polyethylenimine (VWR, Radnor, PA). ..

Article Title: Inhibition of UCH-L1 Deubiquitinating Activity with Two Forms of LDN-57444 Has Anti-Invasive Effects in Metastatic Carcinoma Cells
Article Snippet: .. Transient Transfection Two hundred and ninety-three cells were grown in 100 mm plates and transfected with 3 μg of plasmids with the use of polyethylenimine (VWR, Radnor, PA, USA). ..

Article Title: Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids.
Article Snippet: .. Colorectal cancer (CRC) is characterized by prominent genetic and phenotypic heterogeneity between patients. .. Colorectal cancer (CRC) is characterized by prominent genetic and phenotypic heterogeneity between patients.

Article Title: Exosomal HIF1α supports invasive potential of nasopharyngeal carcinoma-associated LMP1-positive exosomes
Article Snippet: .. Transient transfection and Luciferase assays 293T cells were grown in six-well plates and transfected with 1 μg of plasmids with the use of polyethylenimine (VWR, Radnor, PA, USA). .. Empty vector was used to equalize total amounts of DNA in the transfections. β-Galactosidase served as a transfection control.

Article Title: Crystal Structure of the Heterotrimeric Integrin-Binding Region of Laminin-111
Article Snippet: .. Transfections were performed using polyethylenimine (PEI; VWR International) and a DNA:PEI ratio of 1:3 (w/w). .. The conditioned media containing the secreted heterotrimers were collected 72 hr after transfection.

Luciferase:

Article Title: Exosomal HIF1α supports invasive potential of nasopharyngeal carcinoma-associated LMP1-positive exosomes
Article Snippet: .. Transient transfection and Luciferase assays 293T cells were grown in six-well plates and transfected with 1 μg of plasmids with the use of polyethylenimine (VWR, Radnor, PA, USA). .. Empty vector was used to equalize total amounts of DNA in the transfections. β-Galactosidase served as a transfection control.

Expressing:

Article Title: Sphingosine kinases protect murine embryonic stem cells from sphingosine‐induced cell cycle arrest, et al. Sphingosine kinases protect murine embryonic stem cells from sphingosine‐induced cell cycle arrest
Article Snippet: .. To generate viruses, HEK‐293T cells were plated at 70% confluence in 10‐cm dishes, and for each dish, 3 μg VSVG, 2.5 μg REV, 5 μg RRE, and 15 μg lentiviral destination expression plasmids were combined, then added to a premixed media with 44.8 μL of polyethylenimine in 1.5 mL of DMEM (VWR, Radnor, Pennsylvania), with an additional 7 mL of DMEM for 5 hours. ..

Article Title: Crystallographic analysis of the laminin β2 short arm reveals how the LF domain is inserted into a regular array of LE domains
Article Snippet: .. The cells were transfected with the expression vector using polyethylenimine (PEI; VWR International) using a w /w ratio of DNA:PEI of 1:3. .. The filtered cell culture supernatant was adjusted to a final concentration of 20 mM Na-HEPES (pH 7.5) and loaded onto a 5-ml HisTrap Excel column (GE Healthcare) using an Äkta Purifier (GE Healthcare).

Plasmid Preparation:

Article Title: Crystallographic analysis of the laminin β2 short arm reveals how the LF domain is inserted into a regular array of LE domains
Article Snippet: .. The cells were transfected with the expression vector using polyethylenimine (PEI; VWR International) using a w /w ratio of DNA:PEI of 1:3. .. The filtered cell culture supernatant was adjusted to a final concentration of 20 mM Na-HEPES (pH 7.5) and loaded onto a 5-ml HisTrap Excel column (GE Healthcare) using an Äkta Purifier (GE Healthcare).

Article Title: Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids.
Article Snippet: .. Colorectal cancer (CRC) is characterized by prominent genetic and phenotypic heterogeneity between patients. .. Colorectal cancer (CRC) is characterized by prominent genetic and phenotypic heterogeneity between patients.

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  • 88
    Avantor polyethyleneimine cellulose tlc plate
    Mutagenesis of m1A in E . coli . The base composition at the lesion site for m1A processed in the designated cell strains was obtained by the REAP assay. This <t>polyethyleneimine</t> <t>TLC</t> plate reveals that m1A is weakly mutagenic. The assay reproducibly detected subtle differences in mutagenicity, such as the suppression of A to T mutations by AlkB (center lanes), and the enhancement of such mutations by SOS polymerases (right lanes).
    Polyethyleneimine Cellulose Tlc Plate, supplied by Avantor, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine cellulose tlc plate/product/Avantor
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine cellulose tlc plate - by Bioz Stars, 2020-08
    88/100 stars
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    88
    Avantor polyethyleneimine cellulose plate
    ATPase and helicase assays of recombinant TbPIF5. (A) Coomassie-stained SDS-PAGE gel and Western blot of purified recombinant TbPIF5. (B) Assay of TbPIF5 ATPase activity. The substrates and products were separated by <t>polyethyleneimine</t> thin layer chromatography; arrow shows origin. The [ 32 P]Pi standard in the left-hand lane was prepared from [γ- 32 P] ATP by boiling 5 min in 1 M HCl. (C) Assay of TbPIF5 helicase activity. Substrates and products were separated by 12% polyacrylamide gel electrophoresis. (D) Helicase activity was measured at various concentrations of Mg ++ -ATP. (E) Assay of polarity of TbPIF5 helicase activity. Helicase substrates are diagrammed in Panels C (strand lengths are not to scale) and E (strand lengths for oligonucleotides a, b and c are 21, 21 and 90 nucleotides). * indicates 5′ 32 P end label.
    Polyethyleneimine Cellulose Plate, supplied by Avantor, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine cellulose plate/product/Avantor
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine cellulose plate - by Bioz Stars, 2020-08
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    80
    Avantor polyethyleneimine pei silica
    ATPase and helicase assays of recombinant TbPIF5. (A) Coomassie-stained SDS-PAGE gel and Western blot of purified recombinant TbPIF5. (B) Assay of TbPIF5 ATPase activity. The substrates and products were separated by <t>polyethyleneimine</t> thin layer chromatography; arrow shows origin. The [ 32 P]Pi standard in the left-hand lane was prepared from [γ- 32 P] ATP by boiling 5 min in 1 M HCl. (C) Assay of TbPIF5 helicase activity. Substrates and products were separated by 12% polyacrylamide gel electrophoresis. (D) Helicase activity was measured at various concentrations of Mg ++ -ATP. (E) Assay of polarity of TbPIF5 helicase activity. Helicase substrates are diagrammed in Panels C (strand lengths are not to scale) and E (strand lengths for oligonucleotides a, b and c are 21, 21 and 90 nucleotides). * indicates 5′ 32 P end label.
    Polyethyleneimine Pei Silica, supplied by Avantor, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine pei silica/product/Avantor
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine pei silica - by Bioz Stars, 2020-08
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    85
    Avantor polyethyleneimine pei cellulose thin layer chromatography tlc plates
    ATPase and helicase assays of recombinant TbPIF5. (A) Coomassie-stained SDS-PAGE gel and Western blot of purified recombinant TbPIF5. (B) Assay of TbPIF5 ATPase activity. The substrates and products were separated by <t>polyethyleneimine</t> thin layer chromatography; arrow shows origin. The [ 32 P]Pi standard in the left-hand lane was prepared from [γ- 32 P] ATP by boiling 5 min in 1 M HCl. (C) Assay of TbPIF5 helicase activity. Substrates and products were separated by 12% polyacrylamide gel electrophoresis. (D) Helicase activity was measured at various concentrations of Mg ++ -ATP. (E) Assay of polarity of TbPIF5 helicase activity. Helicase substrates are diagrammed in Panels C (strand lengths are not to scale) and E (strand lengths for oligonucleotides a, b and c are 21, 21 and 90 nucleotides). * indicates 5′ 32 P end label.
    Polyethyleneimine Pei Cellulose Thin Layer Chromatography Tlc Plates, supplied by Avantor, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine pei cellulose thin layer chromatography tlc plates/product/Avantor
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine pei cellulose thin layer chromatography tlc plates - by Bioz Stars, 2020-08
    85/100 stars
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    Mutagenesis of m1A in E . coli . The base composition at the lesion site for m1A processed in the designated cell strains was obtained by the REAP assay. This polyethyleneimine TLC plate reveals that m1A is weakly mutagenic. The assay reproducibly detected subtle differences in mutagenicity, such as the suppression of A to T mutations by AlkB (center lanes), and the enhancement of such mutations by SOS polymerases (right lanes).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli

    doi: 10.1073/pnas.0403489101

    Figure Lengend Snippet: Mutagenesis of m1A in E . coli . The base composition at the lesion site for m1A processed in the designated cell strains was obtained by the REAP assay. This polyethyleneimine TLC plate reveals that m1A is weakly mutagenic. The assay reproducibly detected subtle differences in mutagenicity, such as the suppression of A to T mutations by AlkB (center lanes), and the enhancement of such mutations by SOS polymerases (right lanes).

    Article Snippet: After the DNA was resuspended in 5 μl of 30 mM NaOAc (pH 5.3), 10 mM ZnCl2 , and 1 μg of nuclease P1 (Roche), and incubated at 50°C for 1 h, 0.5-μl samples were spotted 1 cm apart onto a 20 × 20 cm polyethyleneimine cellulose TLC plate (PEI-TLC, J. T. Baker) that had been prewashed in water for 2 min the previous night and air dried.

    Techniques: Mutagenesis, Thin Layer Chromatography

    ATPase and helicase assays of recombinant TbPIF5. (A) Coomassie-stained SDS-PAGE gel and Western blot of purified recombinant TbPIF5. (B) Assay of TbPIF5 ATPase activity. The substrates and products were separated by polyethyleneimine thin layer chromatography; arrow shows origin. The [ 32 P]Pi standard in the left-hand lane was prepared from [γ- 32 P] ATP by boiling 5 min in 1 M HCl. (C) Assay of TbPIF5 helicase activity. Substrates and products were separated by 12% polyacrylamide gel electrophoresis. (D) Helicase activity was measured at various concentrations of Mg ++ -ATP. (E) Assay of polarity of TbPIF5 helicase activity. Helicase substrates are diagrammed in Panels C (strand lengths are not to scale) and E (strand lengths for oligonucleotides a, b and c are 21, 21 and 90 nucleotides). * indicates 5′ 32 P end label.

    Journal: PLoS Pathogens

    Article Title: TbPIF5 Is a Trypanosoma brucei Mitochondrial DNA Helicase Involved in Processing of Minicircle Okazaki Fragments

    doi: 10.1371/journal.ppat.1000589

    Figure Lengend Snippet: ATPase and helicase assays of recombinant TbPIF5. (A) Coomassie-stained SDS-PAGE gel and Western blot of purified recombinant TbPIF5. (B) Assay of TbPIF5 ATPase activity. The substrates and products were separated by polyethyleneimine thin layer chromatography; arrow shows origin. The [ 32 P]Pi standard in the left-hand lane was prepared from [γ- 32 P] ATP by boiling 5 min in 1 M HCl. (C) Assay of TbPIF5 helicase activity. Substrates and products were separated by 12% polyacrylamide gel electrophoresis. (D) Helicase activity was measured at various concentrations of Mg ++ -ATP. (E) Assay of polarity of TbPIF5 helicase activity. Helicase substrates are diagrammed in Panels C (strand lengths are not to scale) and E (strand lengths for oligonucleotides a, b and c are 21, 21 and 90 nucleotides). * indicates 5′ 32 P end label.

    Article Snippet: Samples (1 µl) were spotted onto a polyethyleneimine-cellulose plate (J. T. Baker, USA) and developed in 1.0 M formic acid/0.5 M LiCl followed by autoradiography.

    Techniques: Recombinant, Staining, SDS Page, Western Blot, Purification, Activity Assay, Thin Layer Chromatography, Polyacrylamide Gel Electrophoresis