polyethylenimine linear  (Millipore)


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  • 99
    Name:
    Polyethylenimine linear
    Description:

    Catalog Number:
    765090
    Price:
    None
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    Structured Review

    Millipore polyethylenimine linear
    Polyethylenimine linear

    https://www.bioz.com/result/polyethylenimine linear/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine linear - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Nanobody-Targeted Photodynamic Therapy Selectively Kills Viral GPCR-Expressing Glioblastoma Cells
    Article Snippet: .. The next day, cells were transfected with 100 ng of the different pcDEF3-US28 constructs and adjusted with empty pcDEF3 DNA to a total of 5 μg of DNA and 30 μg of 25 kDa linear polyethylenimine (Sigma-Aldrich) in 150 mM NaCl solution, resulting in a DNA/PEI ratio of 1:6. .. The DNA–PEI mixture was vortexed for 10 s and incubated for 15 min at room temperature (RT).

    Article Title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells
    Article Snippet: .. A pcDNA/FRT‐GAA construct was used to transfect the CHO Flp‐In (cat# R75807, Thermo Fisher Scientific) commercially available cell line that had been previously adapted to grow in chemical defined CD‐CHO media (ThermoFisher) + 8 mM glutamine, using PEI (Polyethylenimine linear, cat#9002‐98‐6 Sigma Aldrich) as a transfection agent and the pOG44 Flp‐Recombinase Expression Vector (cat# V600520 Thermo Fisher Scientific) in a (1:9):3 ratio (3 µg of plasmid DNA added to 27 µg of pOG44, incubated 5 min at RT, followed by 90 µg of linear PEI). .. Colonies that emerged under 250 µg/mL hygromycin B selection pressure were subject to limiting dilution cloning (LDC).

    Article Title: Improved monovalent TNF receptor 1-selective inhibitor with novel heterodimerizing Fc
    Article Snippet: .. All Atrosimab variants and the Fab 13.7 protein were produced in HEK293E cells after transient transfection of a mixture of two vectors, encoding for the different chains of the dimeric molecules using polyethylenimine (linear, 25 kDa, Sigma-Aldrich, Taufkirchen, Germany). .. Proteins were purified by protein A affinity chromatography according to the manufacturers' protocol (TOYOPEARL®, AF-rProtein A-650F, 22805, Tosoh, Stuttgart, Germany).

    Article Title: Circular RNA CircFndc3b modulates cardiac repair after myocardial infarction via FUS/VEGF-A axis
    Article Snippet: .. In brief, HEK293 cells were plated at 1 × 107 cells/15 cm plate one day prior to transfection and polyethyleneimine (Linear PEI, MW 25 kDa, Sigma-Aldrich) was used as the transfection reagent. ..

    Neutralization:

    Article Title: Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes
    Article Snippet: .. Preparation of Neutralization Reagents (NR) Protamine (grade IV, Sigma Aldrich, Darmstadt, Germany), linear (product number 764582, Sigma Aldrich) and branched (product number 408727, Sigma Aldrich) polyethyleneimine (PEI) (Sigma Aldrich), and chitosan (100 kDa, Sigma Aldrich) were used as neutralization and complexation reagents for nucleic acids. .. Neutralization reagents (NRs) were dissolved in ultrapure water or in 0.2 M sodium acetate buffer pH 4.5 (chitosan) at a concentration of 1 mg/mL.

    Construct:

    Article Title: Nanobody-Targeted Photodynamic Therapy Selectively Kills Viral GPCR-Expressing Glioblastoma Cells
    Article Snippet: .. The next day, cells were transfected with 100 ng of the different pcDEF3-US28 constructs and adjusted with empty pcDEF3 DNA to a total of 5 μg of DNA and 30 μg of 25 kDa linear polyethylenimine (Sigma-Aldrich) in 150 mM NaCl solution, resulting in a DNA/PEI ratio of 1:6. .. The DNA–PEI mixture was vortexed for 10 s and incubated for 15 min at room temperature (RT).

    Article Title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells
    Article Snippet: .. A pcDNA/FRT‐GAA construct was used to transfect the CHO Flp‐In (cat# R75807, Thermo Fisher Scientific) commercially available cell line that had been previously adapted to grow in chemical defined CD‐CHO media (ThermoFisher) + 8 mM glutamine, using PEI (Polyethylenimine linear, cat#9002‐98‐6 Sigma Aldrich) as a transfection agent and the pOG44 Flp‐Recombinase Expression Vector (cat# V600520 Thermo Fisher Scientific) in a (1:9):3 ratio (3 µg of plasmid DNA added to 27 µg of pOG44, incubated 5 min at RT, followed by 90 µg of linear PEI). .. Colonies that emerged under 250 µg/mL hygromycin B selection pressure were subject to limiting dilution cloning (LDC).

    Produced:

    Article Title: Improved monovalent TNF receptor 1-selective inhibitor with novel heterodimerizing Fc
    Article Snippet: .. All Atrosimab variants and the Fab 13.7 protein were produced in HEK293E cells after transient transfection of a mixture of two vectors, encoding for the different chains of the dimeric molecules using polyethylenimine (linear, 25 kDa, Sigma-Aldrich, Taufkirchen, Germany). .. Proteins were purified by protein A affinity chromatography according to the manufacturers' protocol (TOYOPEARL®, AF-rProtein A-650F, 22805, Tosoh, Stuttgart, Germany).

    Incubation:

    Article Title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells
    Article Snippet: .. A pcDNA/FRT‐GAA construct was used to transfect the CHO Flp‐In (cat# R75807, Thermo Fisher Scientific) commercially available cell line that had been previously adapted to grow in chemical defined CD‐CHO media (ThermoFisher) + 8 mM glutamine, using PEI (Polyethylenimine linear, cat#9002‐98‐6 Sigma Aldrich) as a transfection agent and the pOG44 Flp‐Recombinase Expression Vector (cat# V600520 Thermo Fisher Scientific) in a (1:9):3 ratio (3 µg of plasmid DNA added to 27 µg of pOG44, incubated 5 min at RT, followed by 90 µg of linear PEI). .. Colonies that emerged under 250 µg/mL hygromycin B selection pressure were subject to limiting dilution cloning (LDC).

    other:

    Article Title: miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
    Article Snippet: After 20 min, the stirring speed was increased to 900 rpm and 3 mL of ammonium hydroxide solution was added to form iron oxide core nanoparticles (magnetic nanoparticles).

    Expressing:

    Article Title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells
    Article Snippet: .. A pcDNA/FRT‐GAA construct was used to transfect the CHO Flp‐In (cat# R75807, Thermo Fisher Scientific) commercially available cell line that had been previously adapted to grow in chemical defined CD‐CHO media (ThermoFisher) + 8 mM glutamine, using PEI (Polyethylenimine linear, cat#9002‐98‐6 Sigma Aldrich) as a transfection agent and the pOG44 Flp‐Recombinase Expression Vector (cat# V600520 Thermo Fisher Scientific) in a (1:9):3 ratio (3 µg of plasmid DNA added to 27 µg of pOG44, incubated 5 min at RT, followed by 90 µg of linear PEI). .. Colonies that emerged under 250 µg/mL hygromycin B selection pressure were subject to limiting dilution cloning (LDC).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells
    Article Snippet: .. A pcDNA/FRT‐GAA construct was used to transfect the CHO Flp‐In (cat# R75807, Thermo Fisher Scientific) commercially available cell line that had been previously adapted to grow in chemical defined CD‐CHO media (ThermoFisher) + 8 mM glutamine, using PEI (Polyethylenimine linear, cat9002‐98‐6 Sigma Aldrich) as a transfection agent and the pOG44 Flp‐Recombinase Expression Vector (cat# V600520 Thermo Fisher Scientific) in a (1:9):3 ratio (3 µg of plasmid DNA added to 27 µg of pOG44, incubated 5 min at RT, followed by 90 µg of linear PEI). .. Colonies that emerged under 250 µg/mL hygromycin B selection pressure were subject to limiting dilution cloning (LDC).

    Plasmid Preparation:

    Article Title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells
    Article Snippet: .. A pcDNA/FRT‐GAA construct was used to transfect the CHO Flp‐In (cat# R75807, Thermo Fisher Scientific) commercially available cell line that had been previously adapted to grow in chemical defined CD‐CHO media (ThermoFisher) + 8 mM glutamine, using PEI (Polyethylenimine linear, cat#9002‐98‐6 Sigma Aldrich) as a transfection agent and the pOG44 Flp‐Recombinase Expression Vector (cat# V600520 Thermo Fisher Scientific) in a (1:9):3 ratio (3 µg of plasmid DNA added to 27 µg of pOG44, incubated 5 min at RT, followed by 90 µg of linear PEI). .. Colonies that emerged under 250 µg/mL hygromycin B selection pressure were subject to limiting dilution cloning (LDC).

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