polyethylenimin  (Millipore)


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  • 98
    Name:
    Polyethylenimine on silica gel
    Description:
    Polyethylenimine PEI is polymer with positive charge density It has various uses like in water purification paper industry to remove nucleic acids from protein solutions and many more This polymer was first used in DNA and oligonucleotides delivery It may also be used as transfection reagent Silica gels are chemically modified with PEI to be used as sorbent PEI stabilizes high surface area solid to adsorb CO2
    Catalog Number:
    246743
    Price:
    None
    Applications:
    Polyethylenimine may be used in separation medium for TLC, paper chromatography and LPLC. Polyethylenimine on silica gel may be used for thin layer chromatography.
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    Structured Review

    Millipore polyethylenimin
    Polyethylenimine on silica gel
    Polyethylenimine PEI is polymer with positive charge density It has various uses like in water purification paper industry to remove nucleic acids from protein solutions and many more This polymer was first used in DNA and oligonucleotides delivery It may also be used as transfection reagent Silica gels are chemically modified with PEI to be used as sorbent PEI stabilizes high surface area solid to adsorb CO2
    https://www.bioz.com/result/polyethylenimin/product/Millipore
    Average 98 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    polyethylenimin - by Bioz Stars, 2020-08
    98/100 stars

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    Related Articles

    Modification:

    Article Title: Proteinuria precedes podocyte abnormalities inLamb2–/– mice, implicating the glomerular basement membrane as an albumin barrier
    Article Snippet: .. Using a method modified from ref. , small kidney cortex pieces were incubated in 0.5% polyethylenimine (1.8 kDa; Sigma-Aldrich), pH 7.3, for 30 minutes on ice, washed, and fixed in 2.5% glutaraldehyde containing 2% phosphotungstic acid for 1 hour on ice. ..

    Incubation:

    Article Title: Proteinuria precedes podocyte abnormalities inLamb2–/– mice, implicating the glomerular basement membrane as an albumin barrier
    Article Snippet: .. Using a method modified from ref. , small kidney cortex pieces were incubated in 0.5% polyethylenimine (1.8 kDa; Sigma-Aldrich), pH 7.3, for 30 minutes on ice, washed, and fixed in 2.5% glutaraldehyde containing 2% phosphotungstic acid for 1 hour on ice. ..

    Small Interfering RNA:

    Article Title: Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells
    Article Snippet: .. Cocultures were investigated for their capability to uptake small interfering RNA (siRNA) by transfecting them with Quasar 570-conjugated hGAPDH siRNA (Riboxx GmbH, Radebeul, Germany) complexed with polyethylenimine (PEI 25 kDa; Sigma-Aldrich), to enhance the siRNA delivery. .. Complexes were prepared by mixing 10 nM PEI to 30 nM hGAPDH siRNA ( N / P = 7) and then incubated for 30 min at room temperature.

    Plasmid Preparation:

    Article Title: Recombinant adeno-associated viruses (rAAV2) facilitate the intraperitoneal gene delivery to cancer cells
    Article Snippet: .. The AAV-293 packaging cells were triple transient co-transfected with rAAV plasmid vectors pRC, pHelper and pGFP/pLacZ at a molar ratio of 1:1:1 using 25 kDa polyethylenimine (PEI 25 kDa; Sigma) cationic polymer as a DNA carrier agent. .. The transfected cells were harvested after 48–72 h. To release rAAV virions, the transfected cells were lysed by the freeze-thaw method.

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  • 99
    Millipore polyethyleneimine cellulose plate
    The RABV L protein generates the mRNA cap structure by an unconventional mechanism: ( a ) The recombinant RABV L protein (0.1 μg) was incubated with the indicated substrates under the standard conditions for the VSV L protein. Nuclease P 1 -digests of RNA products were analyzed by thin layer chromatography on a <t>polyethyleneimine-cellulose</t> plate (PEI-cellulose TLC), followed by autoradiography. The positions of the origin (ori.), guanosine nucleotides (GMP, GDP, GTP), and cap structures (GpppA, GppppA) are shown; ( b ) The recombinant RABV L protein (lane 2, 0.1 μg; lane 3, 0.2 μg) was incubated with [γ- 32 P]GTP. The reaction mixtures were analyzed by PEI-cellulose TLC followed by autoradiography. Lane 1 indicates no enzyme. Lane M shows the position of 32 P-labeled inorganic phosphate (P i ), which was generated by digestion of [γ- 32 P]GTP with alkaline phosphatase.
    Polyethyleneimine Cellulose Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine cellulose plate/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine cellulose plate - by Bioz Stars, 2020-08
    99/100 stars
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    94
    Millipore polyethyleneimine
    Scheme of synthesis of <t>polyethyleneimines</t> (PEI)-n.
    Polyethyleneimine, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine/product/Millipore
    Average 94 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine - by Bioz Stars, 2020-08
    94/100 stars
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    84
    Millipore stearic acid modified branched polyethylenimine
    Scheme of synthesis of <t>polyethyleneimines</t> (PEI)-n.
    Stearic Acid Modified Branched Polyethylenimine, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stearic acid modified branched polyethylenimine/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stearic acid modified branched polyethylenimine - by Bioz Stars, 2020-08
    84/100 stars
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    Image Search Results


    The RABV L protein generates the mRNA cap structure by an unconventional mechanism: ( a ) The recombinant RABV L protein (0.1 μg) was incubated with the indicated substrates under the standard conditions for the VSV L protein. Nuclease P 1 -digests of RNA products were analyzed by thin layer chromatography on a polyethyleneimine-cellulose plate (PEI-cellulose TLC), followed by autoradiography. The positions of the origin (ori.), guanosine nucleotides (GMP, GDP, GTP), and cap structures (GpppA, GppppA) are shown; ( b ) The recombinant RABV L protein (lane 2, 0.1 μg; lane 3, 0.2 μg) was incubated with [γ- 32 P]GTP. The reaction mixtures were analyzed by PEI-cellulose TLC followed by autoradiography. Lane 1 indicates no enzyme. Lane M shows the position of 32 P-labeled inorganic phosphate (P i ), which was generated by digestion of [γ- 32 P]GTP with alkaline phosphatase.

    Journal: Viruses

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity

    doi: 10.3390/v8050144

    Figure Lengend Snippet: The RABV L protein generates the mRNA cap structure by an unconventional mechanism: ( a ) The recombinant RABV L protein (0.1 μg) was incubated with the indicated substrates under the standard conditions for the VSV L protein. Nuclease P 1 -digests of RNA products were analyzed by thin layer chromatography on a polyethyleneimine-cellulose plate (PEI-cellulose TLC), followed by autoradiography. The positions of the origin (ori.), guanosine nucleotides (GMP, GDP, GTP), and cap structures (GpppA, GppppA) are shown; ( b ) The recombinant RABV L protein (lane 2, 0.1 μg; lane 3, 0.2 μg) was incubated with [γ- 32 P]GTP. The reaction mixtures were analyzed by PEI-cellulose TLC followed by autoradiography. Lane 1 indicates no enzyme. Lane M shows the position of 32 P-labeled inorganic phosphate (P i ), which was generated by digestion of [γ- 32 P]GTP with alkaline phosphatase.

    Article Snippet: Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ].

    Techniques: Recombinant, Incubation, Thin Layer Chromatography, Autoradiography, Labeling, Generated

    Scheme of synthesis of polyethyleneimines (PEI)-n.

    Journal: Polymers

    Article Title: Influence of Cross-Linking Degree on Hydrodynamic Behavior and Stimulus-Sensitivity of Derivatives of Branched Polyethyleneimine

    doi: 10.3390/polym12051085

    Figure Lengend Snippet: Scheme of synthesis of polyethyleneimines (PEI)-n.

    Article Snippet: The acetylated derivative of branched polyethyleneimine (PEI-0), namely, poly-N-isobutyroylethyleneimine, was obtained by acylating polyethyleneimine with isobutyroyl chloride under the conditions of the Einhorn reaction (with methylene chloride solvent and triethylamine acceptor).

    Techniques: