polyethyleneimine  (Thermo Fisher)


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    Structured Review

    Thermo Fisher polyethyleneimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethyleneimine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine/product/Thermo Fisher
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes"

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S161426

    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Fluorescence, Mouse Assay, Injection, Proximity Ligation Assay, Blocking Assay

    ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.
    Figure Legend Snippet: ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.

    Techniques Used: Proximity Ligation Assay, Blocking Assay, Electron Paramagnetic Resonance

    Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Incubation, Flow Cytometry, Cytometry, Fluorescence, Proximity Ligation Assay, Laser-Scanning Microscopy, Staining, Blocking Assay

    Characterization of the NPs. ( A ) Particle size (intensity diameter) and zeta potential of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. ( B ) Transmission electron microscopy image of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. The scale bar represents 100 nm. Abbreviations: NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PTX, paclitaxel; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: Characterization of the NPs. ( A ) Particle size (intensity diameter) and zeta potential of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. ( B ) Transmission electron microscopy image of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. The scale bar represents 100 nm. Abbreviations: NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PTX, paclitaxel; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Proximity Ligation Assay, Transmission Assay, Electron Microscopy, Blocking Assay

    ( A ) Synthesis of the PEI-PLA. The PLA-COOH was conjugated to PEI through amide formation in the presence of EDC and NHS, obtaining the cationic amphiphilic copolymer of PEI-PLA. ( B ) 1 H-NMR spectra of PEI and PEI-PLA in D 2 O. The peak of PEI appears at around 2.6 ppm. In PEI-PLA, a new broad peak appears at 2.3–3.4 ppm, which is attributed to the protons of methylene (–CH 2 CH 2 –) in PEI. The signals at δ =1.20 ppm (a) and δ =4.08 ppm (b) correspond to −CH 3 and (–CH) proton in the PLA block of PEI-PLA, respectively. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; NHS, N -hydroxysuccinimide; NMR, nuclear magnetic resonance.
    Figure Legend Snippet: ( A ) Synthesis of the PEI-PLA. The PLA-COOH was conjugated to PEI through amide formation in the presence of EDC and NHS, obtaining the cationic amphiphilic copolymer of PEI-PLA. ( B ) 1 H-NMR spectra of PEI and PEI-PLA in D 2 O. The peak of PEI appears at around 2.6 ppm. In PEI-PLA, a new broad peak appears at 2.3–3.4 ppm, which is attributed to the protons of methylene (–CH 2 CH 2 –) in PEI. The signals at δ =1.20 ppm (a) and δ =4.08 ppm (b) correspond to −CH 3 and (–CH) proton in the PLA block of PEI-PLA, respectively. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; NHS, N -hydroxysuccinimide; NMR, nuclear magnetic resonance.

    Techniques Used: Proximity Ligation Assay, Nuclear Magnetic Resonance, Blocking Assay

    ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Staining, Concentration Assay, Cell Cycle Assay, Proximity Ligation Assay, Blocking Assay

    2) Product Images from "Differential Expression of Syndecan-1 Mediates Cationic Nanoparticle Toxicity in Undifferentiated versus Differentiated Normal Human Bronchial Epithelial Cells"

    Article Title: Differential Expression of Syndecan-1 Mediates Cationic Nanoparticle Toxicity in Undifferentiated versus Differentiated Normal Human Bronchial Epithelial Cells

    Journal: ACS nano

    doi: 10.1021/nn200328m

    Physiocochemical characterization of polyethyleneimine (PEI)-coated MSNPs
    Figure Legend Snippet: Physiocochemical characterization of polyethyleneimine (PEI)-coated MSNPs

    Techniques Used:

    Physiocochemical characterization of polyethyleneimine (PEI)-coated MSNPs
    Figure Legend Snippet: Physiocochemical characterization of polyethyleneimine (PEI)-coated MSNPs

    Techniques Used:

    3) Product Images from "Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes"

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S161426

    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Fluorescence, Mouse Assay, Injection, Proximity Ligation Assay, Blocking Assay

    ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.
    Figure Legend Snippet: ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.

    Techniques Used: Proximity Ligation Assay, Blocking Assay, Electron Paramagnetic Resonance

    Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Incubation, Flow Cytometry, Cytometry, Fluorescence, Proximity Ligation Assay, Laser-Scanning Microscopy, Staining, Blocking Assay

    ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Figure Legend Snippet: ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Techniques Used: Staining, Concentration Assay, Cell Cycle Assay, Proximity Ligation Assay, Blocking Assay

    Related Articles

    Live Cell Imaging:

    Article Title: Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection
    Article Snippet: .. To measure fluid-phase endocytosis and endosome acidification, cells were incubated in Live Cell Imaging Solution (Thermo Fisher Scientific, catalog no. A14291DJ) containing 50 μg/ml FITC-dextran (10,000 Da; Sigma, catalog no. FD10S-100MG) or pHrodo Red-dextran (10,000 Da; Thermo-Fisher Scientific, catalog no. ), respectively, at 37 °C for 5 min, rinsed twice with Live Cell Imaging Solution, and analyzed by spectrofluorometry using a CLARIOstar plate reader with MARS data management software (BMG Labtech). .. Fluorescence signals were normalized according to cell viability determined by PrestoBlue staining as described above.

    Transferring:

    Article Title: The anatomical pathway from the mesodiencephalic junction to the inferior olive relays perioral sensory signals to the cerebellum in the mouse
    Article Snippet: .. Anterograde and retrograde tracer injections Under anaesthesia induced by a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg), a glass pipette filled with 2–3 μl of 10% biotinylated dextran amine (BDA) solution (10,000 MW; Invitrogen, Carlsbad, CA, USA) in PBS (pH 7.4) or Fluoro‐Gold (FG; Wako, Osaka, Japan) dissolved in saline was inserted stereotaxically into the caudal part of the left PfPr (0.6–0.9 mm rostral and 2.2–2.7 mm dorsal to the interaural line, 0.4–0.7 mm lateral to the midline). .. BDA (70 nl) or FG (15 nl) was pressure‐injected using a nanoinjector equipped with a glass microelectrode (Nanoject II), at a flow rate of 23 nl/s.

    Article Title: Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum
    Article Snippet: .. Under anesthesia with isoflurane, a glass pipette filled with a solution of Alexa Fluor 568-conjugated dextran (10% in PBS, 10,000 MW; Thermo Fisher Scientific) was stereotaxically administered to the IO of C57BL/6 mice at P7–P9. .. The tracer was injected iontophoretically with pulse current (10 μA, 700 ms) at 0.5 Hz for 20 min. After tracer injection, the wound was stitched with silk sutures, and mice were moved to a cage with a heating lamp.

    Mouse Assay:

    Article Title: Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum
    Article Snippet: .. Under anesthesia with isoflurane, a glass pipette filled with a solution of Alexa Fluor 568-conjugated dextran (10% in PBS, 10,000 MW; Thermo Fisher Scientific) was stereotaxically administered to the IO of C57BL/6 mice at P7–P9. .. The tracer was injected iontophoretically with pulse current (10 μA, 700 ms) at 0.5 Hz for 20 min. After tracer injection, the wound was stitched with silk sutures, and mice were moved to a cage with a heating lamp.

    Incubation:

    Article Title: Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection
    Article Snippet: .. To measure fluid-phase endocytosis and endosome acidification, cells were incubated in Live Cell Imaging Solution (Thermo Fisher Scientific, catalog no. A14291DJ) containing 50 μg/ml FITC-dextran (10,000 Da; Sigma, catalog no. FD10S-100MG) or pHrodo Red-dextran (10,000 Da; Thermo-Fisher Scientific, catalog no. ), respectively, at 37 °C for 5 min, rinsed twice with Live Cell Imaging Solution, and analyzed by spectrofluorometry using a CLARIOstar plate reader with MARS data management software (BMG Labtech). .. Fluorescence signals were normalized according to cell viability determined by PrestoBlue staining as described above.

    other:

    Article Title: FYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate microtubule plus end-directed vesicle transport
    Article Snippet: LysoTracker red and Alexa Fluor 647–dextran (10,000 D) were obtained from Invitrogen.

    Injection:

    Article Title: HMG-CoA Reductase Inhibition Promotes Neurological Recovery, Peri-Lesional Tissue Remodeling, and Contralesional Pyramidal Tract Plasticity after Focal Cerebral Ischemia
    Article Snippet: .. For this purpose, the anterograde tract tracers cascade-blue-labeled dextran amine [cascade blue (CB)] or biotinylated dextran amine (BDA) (both 10,000 MW; Molecular Probes, Eugene, OR, USA) were injected into the ipsilesional or contralesional motor cortices, respectively, at 42 dpi or 42 days post-sham surgery (Reitmeir et al., , ). ..

    Software:

    Article Title: Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection
    Article Snippet: .. To measure fluid-phase endocytosis and endosome acidification, cells were incubated in Live Cell Imaging Solution (Thermo Fisher Scientific, catalog no. A14291DJ) containing 50 μg/ml FITC-dextran (10,000 Da; Sigma, catalog no. FD10S-100MG) or pHrodo Red-dextran (10,000 Da; Thermo-Fisher Scientific, catalog no. ), respectively, at 37 °C for 5 min, rinsed twice with Live Cell Imaging Solution, and analyzed by spectrofluorometry using a CLARIOstar plate reader with MARS data management software (BMG Labtech). .. Fluorescence signals were normalized according to cell viability determined by PrestoBlue staining as described above.

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