Structured Review

Fisher Scientific polyethyleneimine
Polyethyleneimine, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethyleneimine/product/Fisher Scientific
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
polyethyleneimine - by Bioz Stars, 2020-08
92/100 stars

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Transfection:

Article Title: PKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels
Article Snippet: .. For transient transfection, polyethylenimine (PEI; Fisher Scientific, Waltham, MA) was used as DNA carrier. .. Briefly, PEI and DNA were mixed in Opti-MEM (Mediatech) at a ratio of 4:1.

Article Title: Generation of an Optimized Lentiviral Vector Encoding a High-Expression Factor VIII Transgene for Gene Therapy of Hemophilia A
Article Snippet: .. Cell transfections were performed with polyethyleneimine purchased from Fisher Scientific (Pittsburg, PA). .. Plasmids utilized for viral preparation were isolated from bacterial stocks utilizing QIAGEN Hispeed midiprep plasmid kits (Valencia, CA).

Article Title: Frog Virus 3 Open Reading Frame 97R Localizes to the Endoplasmic Reticulum and Induces Nuclear Invaginations
Article Snippet: .. In a sterile, 1.5-ml centrifuge tube, 200 μl of serum-free DMEM (Thermo Scientific), 20 μl of 50 mg/μl polyethylenimine (PEI; Fisher Scientific, Ottawa, ON, Canada), and 4 μl of 10 μg/μl plasmid DNA were mixed and left to incubate at room temperature for 15 min. Transfection complexes were then added to the cells with 1 ml fresh medium. ..

other:

Article Title: Assessment of sulfonated homo and co-polyimides incorporated polysulfone ultrafiltration blend membranes for effective removal of heavy metals and proteins
Article Snippet: Concentrated sulfuric acid (95%), fuming sulfuric acid (SO3 , 60%), m -cresol and polyethyleneimine (Mn ~60 kDa) were purchased from Fisher Scientific, and used as received.

Article Title: Assessment of sulfonated homo and co-polyimides incorporated polysulfone ultrafiltration blend membranes for effective removal of heavy metals and proteins.
Article Snippet: Concentrated sulfuric acid (95%), fuming sulfuric acid (SO3, 60%), m-cresol and polyethyleneimine (Mn ~60 kDa) were purchased from Fisher Scientific, and used as received.

Cross-linking Immunoprecipitation:

Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways
Article Snippet: .. For and , pHR plasmid encoding CLIP-tagged full length human PD-L1 (CLIP–PD-L1) or PD-L2 (CLIP–PD-L2) was co-transfected with pHR encoding either SNAP-tagged full length human CD80 (SNAP–CD80) or CD86 (SNAP–CD86) into HEK293T cells using polyethylenimine (Fisher Scientific, NC1014320). .. 72 h post transfection, cells were trypsinized and seeded on poly-D-lysine (Sigma-Aldrich, P6407) treated 96 wells plate with a glass bottom (Dot Scientific, MGB096-1-2-LG-L).

Plasmid Preparation:

Article Title: Frog Virus 3 Open Reading Frame 97R Localizes to the Endoplasmic Reticulum and Induces Nuclear Invaginations
Article Snippet: .. In a sterile, 1.5-ml centrifuge tube, 200 μl of serum-free DMEM (Thermo Scientific), 20 μl of 50 mg/μl polyethylenimine (PEI; Fisher Scientific, Ottawa, ON, Canada), and 4 μl of 10 μg/μl plasmid DNA were mixed and left to incubate at room temperature for 15 min. Transfection complexes were then added to the cells with 1 ml fresh medium. ..

Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways
Article Snippet: .. For and , pHR plasmid encoding CLIP-tagged full length human PD-L1 (CLIP–PD-L1) or PD-L2 (CLIP–PD-L2) was co-transfected with pHR encoding either SNAP-tagged full length human CD80 (SNAP–CD80) or CD86 (SNAP–CD86) into HEK293T cells using polyethylenimine (Fisher Scientific, NC1014320). .. 72 h post transfection, cells were trypsinized and seeded on poly-D-lysine (Sigma-Aldrich, P6407) treated 96 wells plate with a glass bottom (Dot Scientific, MGB096-1-2-LG-L).

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  • 92
    Fisher Scientific polyethyleneimine
    Polyethyleneimine, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine/product/Fisher Scientific
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific polyethylenimine pei
    Polyethylenimine Pei, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethylenimine pei/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyethylenimine pei - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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