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Poly Scientific Inc polyethyleneimine pei
Polyethyleneimine Pei, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyethyleneimine pei/product/Poly Scientific Inc
Average 91 stars, based on 3 article reviews
Price from $9.99 to $1999.99
polyethyleneimine pei - by Bioz Stars, 2020-09
91/100 stars

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Plasmid Preparation:

Article Title: Characterization, modelling and mitigation of gene expression burden in mammalian cells
Article Snippet: .. The transfection solution was prepared using Polyethylenimine (PEI) “MAX” (Mw 40000; Polysciences, Inc.) in a 1:3 (ug DNA to ug PEI) ratio with a total of 500 ng of plasmid DNA per well. .. Both DNA and PEI were diluted in Opti-MEM I reduced serum media (Gibco) before being mixed and incubated for 25 minutes prior to addition to the cells.

Article Title: Hypoxia-inducible lipid droplet-associated interacts with DGAT1 to promote lipid storage in hepatocytes
Article Snippet: .. Transfections were performed with 800 ng of single or 1600 ng of mixed plasmid DNA complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM. .. After 5 h, the medium was replaced by serum free DMEM supplemented with 1% fatty acid free BSA (Roche Applied Sciences) and a mixture of oleate and palmitate (ratio 2:1, total concentration 0.8 mM) and left overnight.

Article Title: SVEP1, a novel human coronary artery disease locus, promotes atherosclerosis
Article Snippet: .. Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 . .. After overnight incubation, the same volume of fresh media plus 1 mM (final concentration) valproic acid was added.

Transfection:

Article Title: Characterization, modelling and mitigation of gene expression burden in mammalian cells
Article Snippet: .. The transfection solution was prepared using Polyethylenimine (PEI) “MAX” (Mw 40000; Polysciences, Inc.) in a 1:3 (ug DNA to ug PEI) ratio with a total of 500 ng of plasmid DNA per well. .. Both DNA and PEI were diluted in Opti-MEM I reduced serum media (Gibco) before being mixed and incubated for 25 minutes prior to addition to the cells.

Article Title: Hypoxia-inducible lipid droplet-associated interacts with DGAT1 to promote lipid storage in hepatocytes
Article Snippet: .. Transfections were performed with 800 ng of single or 1600 ng of mixed plasmid DNA complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM. .. After 5 h, the medium was replaced by serum free DMEM supplemented with 1% fatty acid free BSA (Roche Applied Sciences) and a mixture of oleate and palmitate (ratio 2:1, total concentration 0.8 mM) and left overnight.

Article Title: Global proteomics of Ubqln2-based murine models of ALS
Article Snippet: .. APEX2 labelling and purification UBQLN1 , 2 , and 4 TKO HEK 293 cells were transfected with UBQLN1 or UBQLN2 APEX2 constructs using polyethylenimine (PEI) at a 3:1 PEI:DNA ratio (Polysciences, Inc.). .. APEX labeling and subsequent streptavidin pull-downs were performed as described in ( ).

Article Title: 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function
Article Snippet: .. Polyethyleneimine (PEI) (Polysciences, Inc.) was used as a transfection reagent as per the manufacturer’s instructions. .. To perform the immunofluorescence and Western blot assays, cells seeded on a cover-slip in a 35 mm dish were co-transfected with 1µg of the mOrange 14-3-3γ constructs and 1µg of the EGFP-Centrin2 or FLAG NPM1 or ECFP NPM1 constructs.

Article Title: SVEP1, a novel human coronary artery disease locus, promotes atherosclerosis
Article Snippet: .. Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 . .. After overnight incubation, the same volume of fresh media plus 1 mM (final concentration) valproic acid was added.

Article Title: Synergistic reduction of HIV-1 infectivity by 5-azacytidine and inhibitors of ribonucleotide reductase.
Article Snippet: .. Although many compounds have been approved for the treatment of human immunodeficiency type-1 (HIV-1) infection, additional anti-HIV-1 drugs (particularly those belonging to new drug classes) are still needed due to issues such as long-term drug-associated toxicities, transmission of drug-resistant variants, and development of multi-class resistance. .. Although many compounds have been approved for the treatment of human immunodeficiency type-1 (HIV-1) infection, additional anti-HIV-1 drugs (particularly those belonging to new drug classes) are still needed due to issues such as long-term drug-associated toxicities, transmission of drug-resistant variants, and development of multi-class resistance.

Incubation:

Article Title: Structure-based validation can drastically under-estimate error rate in proteome-wide cross-linking mass spectrometry studies
Article Snippet: .. When reaching 60-70% confluency, the cells were co-transfected with both plasmids containing the Venus fragments-tagged bait and prey ORF (100 ng for each) which were pre-mixed and incubated with polyethylenimine (PEI) (Polysciences Inc.) and OptiMEM (Gibco). ..

Construct:

Article Title: Global proteomics of Ubqln2-based murine models of ALS
Article Snippet: .. APEX2 labelling and purification UBQLN1 , 2 , and 4 TKO HEK 293 cells were transfected with UBQLN1 or UBQLN2 APEX2 constructs using polyethylenimine (PEI) at a 3:1 PEI:DNA ratio (Polysciences, Inc.). .. APEX labeling and subsequent streptavidin pull-downs were performed as described in ( ).

Purification:

Article Title: Global proteomics of Ubqln2-based murine models of ALS
Article Snippet: .. APEX2 labelling and purification UBQLN1 , 2 , and 4 TKO HEK 293 cells were transfected with UBQLN1 or UBQLN2 APEX2 constructs using polyethylenimine (PEI) at a 3:1 PEI:DNA ratio (Polysciences, Inc.). .. APEX labeling and subsequent streptavidin pull-downs were performed as described in ( ).

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    Poly Scientific Inc pei 25k
    Pei 25k, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 88/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Poly Scientific Inc polyethyleneimine pei
    Polyethyleneimine Pei, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyethyleneimine pei/product/Poly Scientific Inc
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    polyethyleneimine pei - by Bioz Stars, 2020-09
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    Poly Scientific Inc polyethylenimine
    Polyethylenimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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