polyclonal rabbit anti human stmn1 ser63 antibody  (Abcam)

 
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    Abcam polyclonal rabbit anti human stmn1 ser63 antibody
    Polyclonal Rabbit Anti Human Stmn1 Ser63 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti human stmn1 ser63 antibody/product/Abcam
    Average 88 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti human stmn1 ser63 antibody - by Bioz Stars, 2020-09
    88/100 stars

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    Staining:

    Article Title: CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma
    Article Snippet: .. Immunohistochemistry After blocking with 5% goat serum (OriGene Technologies, Inc., Beijing, China) for 30 min at room temperature, immunohistochemical staining was performed on the TMAs at a section thickness of 5 µm using rabbit polyclonal anti-CIZ1 antibody (cat. no. ab102013, Abcam, Cambridge, MA, USA) with a dilution of 1:600 at 4°C overnight. .. Following washing with 1X phosphate-buffered saline, the sections were incubated with biotinylated anti-mouse secondary antibody (cat. no. TA130008; OriGene Technologies, Inc.; dilution, 1:100) for 30 mins at room temperature, followed by the streptavidin-peroxidase method according to the manufacturer's protocol (A0303; Beyotime Institute of Biotechnology, Shanghai, China).

    Article Title: A COEUR cohort study of SATB2 expression and its prognostic value in ovarian endometrioid carcinoma
    Article Snippet: .. The TMAs were stained with antibodies routinely used in clinical pathology for diagnosis of ovarian tumors: SATB2 (clone EPNCIR130A, Abcam, Toronto, ON, Canada), CDX2 (EPR2764Y, Cell Marque, Rocklin, CA, USA) and PR (clone 1E2, Roche, Laval, QC, Canada). ..

    Immunohistochemistry:

    Article Title: CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma
    Article Snippet: .. Immunohistochemistry After blocking with 5% goat serum (OriGene Technologies, Inc., Beijing, China) for 30 min at room temperature, immunohistochemical staining was performed on the TMAs at a section thickness of 5 µm using rabbit polyclonal anti-CIZ1 antibody (cat. no. ab102013, Abcam, Cambridge, MA, USA) with a dilution of 1:600 at 4°C overnight. .. Following washing with 1X phosphate-buffered saline, the sections were incubated with biotinylated anti-mouse secondary antibody (cat. no. TA130008; OriGene Technologies, Inc.; dilution, 1:100) for 30 mins at room temperature, followed by the streptavidin-peroxidase method according to the manufacturer's protocol (A0303; Beyotime Institute of Biotechnology, Shanghai, China).

    Incubation:

    Article Title: Tenascin-C: A Novel Candidate Marker for Cancer Stem Cells in Glioblastoma Identified by Tissue Microarrays
    Article Snippet: .. The TMAs were then rinsed with water and PBS and subsequently blocked with 2% BSA and incubated with rabbit anti-human Tenascin-C monoclonal antibody (1:100 dilution, Abcam, Cambridge, MA) overnight at 4 °C followed by incubation with a goat anti-rabbit IgG conjugated to horseradish peroxidase (1:250 Abcam, Cambridge, MA). .. Immunodetection was performed using DAB solution (Vector Laboratories, Burlingame, CA).

    Article Title: NRSF/REST levels are decreased in cholangiocellular carcinoma but not hepatocellular carcinoma compared with normal liver tissues: A tissue microarray study
    Article Snippet: .. Subsequently, the TMAs were incubated overnight at 4°C with a polyclonal rabbit antibody against NRSF/REST (cat. no. ab21635; Abcam, Cambridge, UK) that was diluted 1:100 with antibody diluent (cat. no. S3022; Dako; Agilent Technologies, Inc., Santa Clara, CA, USA). .. Following several washes with PBS, the sections were incubated with biotinylated goat anti-rabbit secondary antibody (cat. no. ZB2010; 1:200; Beijing Zhongshan, China) for 1 h at room temperature.

    Article Title: High expression of endothelial progenitor cell-induced angiogenic markers is associated with bile acid levels in HCC
    Article Snippet: .. After washing, TMAs were incubated with a horseradish peroxidase-conjugated secondary antibody (1:1,000; cat. no. ab6789; Abcam) for 30 min at 37°C, the color was developed by a 15 min incubation at room temperature with DAB solution (cat. no. E670033; Sangon Biotech Co., Ltd.) and then the sections were weakly counterstained with hematoxylin for 10 min at room temperature. ..

    Article Title: Prognostic Value of Rab27B Nuclear Expression in Gastrointestinal Stromal Tumors
    Article Snippet: .. The TMAs were incubated for 1 h with primary anti-Rab27B antibody (1 : 100 dilution, ab104083; Abcam, Cambridge, MA, USA), washed, and incubated with an anti-rabbit horseradish-peroxidase-conjugated antibody. .. As a negative control, phosphate-buffered saline was used instead of the primary antibody.

    Article Title: Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients
    Article Snippet: .. For the other three markers, TMAs were incubated with polyclonal rabbit anti-human STMN1 Ser16 antibody (Abcam), polyclonal rabbit anti-human STMN1 Ser25 antibody (Novus), and polyclonal rabbit anti-human STMN1 Ser63 antibody (Abcam) diluted to 1:50 in Bond Primary Diluent in a humid chamber at 40°C overnight. .. Following washes with PBS, all of the TMAs were incubated for 30 min with secondary antibody (GTVision™III Detection System/Mo & Rb) at RT.

    Expressing:

    Article Title: Musashi-2 promotes hepatitis Bvirus related hepatocellular carcinoma progression via the Wnt/β-catenin pathway
    Article Snippet: .. MSI2 and Ki-67 expression were detected on the TMAs following citrate buffer (pH 6.0) antigen retrieval using standard methodology and a primary antibody against MSI2 (1:80 dilution, Abcam, USA) or Ki-67 (1:50 dilution, Dako Cytomation, Copenhagen, Denmark).Tissue sections were counterstained with Mayer’s Hematoxylin. .. Positive staining was scored by two independent investigators without the knowledge of patient outcomes (double-blind) according to the stained area.

    Blocking Assay:

    Article Title: CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma
    Article Snippet: .. Immunohistochemistry After blocking with 5% goat serum (OriGene Technologies, Inc., Beijing, China) for 30 min at room temperature, immunohistochemical staining was performed on the TMAs at a section thickness of 5 µm using rabbit polyclonal anti-CIZ1 antibody (cat. no. ab102013, Abcam, Cambridge, MA, USA) with a dilution of 1:600 at 4°C overnight. .. Following washing with 1X phosphate-buffered saline, the sections were incubated with biotinylated anti-mouse secondary antibody (cat. no. TA130008; OriGene Technologies, Inc.; dilution, 1:100) for 30 mins at room temperature, followed by the streptavidin-peroxidase method according to the manufacturer's protocol (A0303; Beyotime Institute of Biotechnology, Shanghai, China).

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  • 88
    Abcam polyclonal rabbit anti human stmn1 ser16 antibody
    Polyclonal Rabbit Anti Human Stmn1 Ser16 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti human stmn1 ser16 antibody/product/Abcam
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti human stmn1 ser16 antibody - by Bioz Stars, 2020-09
    88/100 stars
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