Structured Review

Abcam anti α7 monoclonal ab
Detection an <t>α7</t> calcium current in the growth cone. Cells were analyzed for intracellular calcium changes using Fluo-8AM. (a) Calcium detection in soma, axon, central zone, and filopodia of hippocampal neurons. Normalized traces were obtained
Anti α7 Monoclonal Ab, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti α7 monoclonal ab/product/Abcam
Average 85 stars, based on 6 article reviews
Price from $9.99 to $1999.99
anti α7 monoclonal ab - by Bioz Stars, 2021-01
85/100 stars

Images

1) Product Images from "Axon Targeting of the Alpha 7 Nicotinic Receptor in Developing Hippocampal Neurons by Gprin1 Regulates Growth"

Article Title: Axon Targeting of the Alpha 7 Nicotinic Receptor in Developing Hippocampal Neurons by Gprin1 Regulates Growth

Journal: Journal of neurochemistry

doi: 10.1111/jnc.12641

Detection an α7 calcium current in the growth cone. Cells were analyzed for intracellular calcium changes using Fluo-8AM. (a) Calcium detection in soma, axon, central zone, and filopodia of hippocampal neurons. Normalized traces were obtained
Figure Legend Snippet: Detection an α7 calcium current in the growth cone. Cells were analyzed for intracellular calcium changes using Fluo-8AM. (a) Calcium detection in soma, axon, central zone, and filopodia of hippocampal neurons. Normalized traces were obtained

Techniques Used:

α7 and Gprin1 interact in the developing hippocampus. (a) Coronal slices of brains obtained from P5 and P30 rats. Slices were co-labeled with fBgtx and anti-Gprin1 Abs and visualized throughout the hippocampus, with prominent expression found
Figure Legend Snippet: α7 and Gprin1 interact in the developing hippocampus. (a) Coronal slices of brains obtained from P5 and P30 rats. Slices were co-labeled with fBgtx and anti-Gprin1 Abs and visualized throughout the hippocampus, with prominent expression found

Techniques Used: Labeling, Expressing

α7 and Gprin1 associate in the growth cone. (a) Hippocampal neurons from P0 pups was cultured for 3 DIV. Neurons were probed with fBgtx (green) and anti-Gprin1 Abs (red) (top images). A heat-map measure of the co-signal (bottom image) shows the
Figure Legend Snippet: α7 and Gprin1 associate in the growth cone. (a) Hippocampal neurons from P0 pups was cultured for 3 DIV. Neurons were probed with fBgtx (green) and anti-Gprin1 Abs (red) (top images). A heat-map measure of the co-signal (bottom image) shows the

Techniques Used: Cell Culture

Activation of α7 inhibits axon growth. Hippocampal neurons were treated with multiple doses of PNU or a 0.1% DMSO control (PNU=0). (a) Analysis of surface area (SA) and branch number of Tau-1+ axons (left) represented as phase contrast images
Figure Legend Snippet: Activation of α7 inhibits axon growth. Hippocampal neurons were treated with multiple doses of PNU or a 0.1% DMSO control (PNU=0). (a) Analysis of surface area (SA) and branch number of Tau-1+ axons (left) represented as phase contrast images

Techniques Used: Activation Assay

α7 mediated inhibition of axon growth is dependent on a Gprin1 pathway. (a) An analysis of axon and GC morphology in cells treated with 10 µM PNU. Values are based on average percent change in axon growth from control cells (cells transfected
Figure Legend Snippet: α7 mediated inhibition of axon growth is dependent on a Gprin1 pathway. (a) An analysis of axon and GC morphology in cells treated with 10 µM PNU. Values are based on average percent change in axon growth from control cells (cells transfected

Techniques Used: Inhibition, Transfection

Choline activation of α7 in the growth cone. Cells were analyzed for intracellular calcium changes using Fluo-8AM in response to Choline (Ch) treatment. Ch=1 mM; Bgtx=50 µM. (a) Calcium detection in soma, axon, central zone, and filopodia
Figure Legend Snippet: Choline activation of α7 in the growth cone. Cells were analyzed for intracellular calcium changes using Fluo-8AM in response to Choline (Ch) treatment. Ch=1 mM; Bgtx=50 µM. (a) Calcium detection in soma, axon, central zone, and filopodia

Techniques Used: Activation Assay

Gprin1 directs the localization of α7 to the cell surface of the growth cone. (a) pRNAT H1.1/Gprin1- cells were labeled with fBgtx for the visualization of α7 expression. Right panels: magnified images of the GC showing changes in α7
Figure Legend Snippet: Gprin1 directs the localization of α7 to the cell surface of the growth cone. (a) pRNAT H1.1/Gprin1- cells were labeled with fBgtx for the visualization of α7 expression. Right panels: magnified images of the GC showing changes in α7

Techniques Used: Labeling, Expressing

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Isolation:

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Article Snippet: .. Sumoylation assay using an ELISA-based kit Cytosolic and membrane proteins were isolated using standard protein isolation protocols and kits (Abcam). .. Relative sumoylation levels under hypoxia were measured using an In Vivo Protein Sumoylation Assay Ultra kit (EpiQuik).

Article Title: Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge’s disease
Article Snippet: .. IP, immunoblotting, and sumoylation ratios Cytosolic and membrane proteins were isolated using standard protein isolation protocols and kits (Abcam). .. IP was performed using an IP kit (Thermo Fisher Scientific) following the procedure described by .

Article Title: Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation
Article Snippet: .. An aliquot of the isolated membrane protein was subjected to western blotting for CD39 (#ab108248, Abcam) with Na+ /K+ ATPase used as a reference (#58475, Abcam). .. Immunofluorescence Staining and Flow Cytometry Analysis of CD39 Surface-exposed CD39 on BMDCs was detected by surface staining.

Article Title: Platelet-derived SDF1 primes pulmonary capillary vascular niche to drive lung alveolar regeneration
Article Snippet: .. Protein level of MMP14 in isolated membrane proteins after PNX was determined by immunoblot (Abcam), and EC-specific marker VE-cadherin was also blotted (R & D systems) as control for loading of endothelial membrane proteins. .. To control for the purity of isolated EC memebrane proteins, endothelial membrane proteins such as platelet-endothelial cell adhesion molecule (PECAM)-1 (Santa Cruz Biotechnology, sc-1506, 10 μg/ml), intercellular cell adhesion molecule (ICAM)-1 (Santa Cruz Biotechnology, sc-1511, 10 μg/ml), VEGF receptor 2 (VEGFR2), angiotensin converting enzyme (sc-20998, 2 μg/ml), integrin αv (sc-6618, 5 μg/ml), α5 (sc-10729, 5 μg/ml), E-cadherin (sc-7870, 10 μg/ml), and cytoplasmic proteins α-tubulin (sc-53646, 10 μg/ml), and β-actin were also examined in the isolated membrane fractions by immunoblot as described above.

Enzyme-linked Immunosorbent Assay:

Article Title: Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge’s disease
Article Snippet: .. Sumoylation assay using an ELISA-based kit Cytosolic and membrane proteins were isolated using standard protein isolation protocols and kits (Abcam). .. Relative sumoylation levels under hypoxia were measured using an In Vivo Protein Sumoylation Assay Ultra kit (EpiQuik).

Protein Extraction:

Article Title: ERαΔ4, an ERα splice variant missing exon4, interacts with caveolin-3 and mGluR2/3
Article Snippet: .. Membrane proteins were extracted from ARH tissue using the Plasma Membrane Protein Extraction Kit (ab65400; Abcam, Cambridge, MA) according to the manufacturer’s protocol. .. Briefly, tissues were homogenized in 2 volumes of 1X Homogenize Buffer with protease inhibitors until completely lysed (30 times).

Marker:

Article Title: Platelet-derived SDF1 primes pulmonary capillary vascular niche to drive lung alveolar regeneration
Article Snippet: .. Protein level of MMP14 in isolated membrane proteins after PNX was determined by immunoblot (Abcam), and EC-specific marker VE-cadherin was also blotted (R & D systems) as control for loading of endothelial membrane proteins. .. To control for the purity of isolated EC memebrane proteins, endothelial membrane proteins such as platelet-endothelial cell adhesion molecule (PECAM)-1 (Santa Cruz Biotechnology, sc-1506, 10 μg/ml), intercellular cell adhesion molecule (ICAM)-1 (Santa Cruz Biotechnology, sc-1511, 10 μg/ml), VEGF receptor 2 (VEGFR2), angiotensin converting enzyme (sc-20998, 2 μg/ml), integrin αv (sc-6618, 5 μg/ml), α5 (sc-10729, 5 μg/ml), E-cadherin (sc-7870, 10 μg/ml), and cytoplasmic proteins α-tubulin (sc-53646, 10 μg/ml), and β-actin were also examined in the isolated membrane fractions by immunoblot as described above.

Western Blot:

Article Title: Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation
Article Snippet: .. An aliquot of the isolated membrane protein was subjected to western blotting for CD39 (#ab108248, Abcam) with Na+ /K+ ATPase used as a reference (#58475, Abcam). .. Immunofluorescence Staining and Flow Cytometry Analysis of CD39 Surface-exposed CD39 on BMDCs was detected by surface staining.

Article Title: Functional Study of Novel Bartter’s Syndrome Mutations in ClC-Kb and Rescue by the Accessory Subunit Barttin Toward Personalized Medicine
Article Snippet: .. Surface proteins were separated on a SDS-PAGE gel and the samples (10 µg) were analyzed by western blotting using a monoclonal anti-ClC-Kb antibody (Abcam, ab66460). .. Filters were also immunoblotted with β-actin monoclonal antibody (Sigma) as control.

SDS Page:

Article Title: Functional Study of Novel Bartter’s Syndrome Mutations in ClC-Kb and Rescue by the Accessory Subunit Barttin Toward Personalized Medicine
Article Snippet: .. Surface proteins were separated on a SDS-PAGE gel and the samples (10 µg) were analyzed by western blotting using a monoclonal anti-ClC-Kb antibody (Abcam, ab66460). .. Filters were also immunoblotted with β-actin monoclonal antibody (Sigma) as control.

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    Abcam rabbit polyclonal anti gprin1 ab
    Rabbit Polyclonal Anti Gprin1 Ab, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti gprin1 ab/product/Abcam
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti gprin1 ab - by Bioz Stars, 2021-01
    86/100 stars
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