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Santa Cruz Biotechnology polyclonal primary antibody against keap1
Polyclonal Primary Antibody Against Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal primary antibody against keap1/product/Santa Cruz Biotechnology
Average 86 stars, based on 2 article reviews
Price from $9.99 to $1999.99
polyclonal primary antibody against keap1 - by Bioz Stars, 2020-04
86/100 stars

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Protease Inhibitor:

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: Cell lysates (6 mg protein) containing protease inhibitor cocktail were added to the beads and incubated for 3 h at room temperature with rotation. .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz).

Incubation:

Article Title: Accumulation of 15-deoxy-?12,14-prostaglandin J2 adduct formation with Keap1 over time: effects on potency for intracellular antioxidant defense induction
Article Snippet: .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membrane at 100V for 2h and membranes blocked with 5% skimmed milk in TBS-T. Membranes were incubated with polyclonal primary antibody against Keap1 (Santa Cruz: E-20 1:1000 dilution in 5% skimmed milk in TBS-T), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (Santa Cruz; 1:1000 dilution in 5% skimmed milk in TBS-T). .. Membranes were developed by chemiluminescence using SuperSignal West Dura substrate (Pierce, Rockford, IL) and sequential images taken with quantitation only performed on bands which had not reached saturation as described in [ ].

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz). .. Membranes were developed by chemiluminescence using SuperSignal West Dura substrate (Pierce) and sequential images taken with quantitation only performed on bands which had not reached saturation.

Labeling:

Article Title: Accumulation of 15-deoxy-?12,14-prostaglandin J2 adduct formation with Keap1 over time: effects on potency for intracellular antioxidant defense induction
Article Snippet: Samples were then prepared for analysis by heating the beads to 80°C for 10 min in 80 μl of 2x sample buffer (0.1 M Tris, 4% SDS, 10% Glycerol, 0.2% Bromophenol blue, pH 6.8) containing β-mercaptoethanol to release the biotin labeled proteins. .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membrane at 100V for 2h and membranes blocked with 5% skimmed milk in TBS-T. Membranes were incubated with polyclonal primary antibody against Keap1 (Santa Cruz: E-20 1:1000 dilution in 5% skimmed milk in TBS-T), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (Santa Cruz; 1:1000 dilution in 5% skimmed milk in TBS-T).

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: Samples were then prepared for analysis by heating the beads to 80°C for 10 min in 80 μl of 5x sample buffer (0.5 M Tris, 20% SDS, 50% Glycerol, 1% Bromophenol blue, pH 6.8) containing β-mercaptoethanol to release the biotin labeled proteins. .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz).

Quantitation Assay:

Article Title: Accumulation of 15-deoxy-?12,14-prostaglandin J2 adduct formation with Keap1 over time: effects on potency for intracellular antioxidant defense induction
Article Snippet: Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membrane at 100V for 2h and membranes blocked with 5% skimmed milk in TBS-T. Membranes were incubated with polyclonal primary antibody against Keap1 (Santa Cruz: E-20 1:1000 dilution in 5% skimmed milk in TBS-T), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (Santa Cruz; 1:1000 dilution in 5% skimmed milk in TBS-T). .. Membranes were developed by chemiluminescence using SuperSignal West Dura substrate (Pierce, Rockford, IL) and sequential images taken with quantitation only performed on bands which had not reached saturation as described in [ ].

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz). .. Membranes were developed by chemiluminescence using SuperSignal West Dura substrate (Pierce) and sequential images taken with quantitation only performed on bands which had not reached saturation.

Modification:

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: Paragraph title: Determination of the modification of Keap1 and β-actin by 15d-PGJ2 ... Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz).

Lysis:

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: To determine the extent of modification of Keap1 by 15d-PGJ2 , JC cells were treated with increasing concentrations (0.3-20 μM) of bt-15d-PGJ2 for 4 h. After the treatment, cell lysates were prepared in 1% Triton X-100 in Tris-HCl (10 mM) lysis buffer. .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz).

SDS Page:

Article Title: Accumulation of 15-deoxy-?12,14-prostaglandin J2 adduct formation with Keap1 over time: effects on potency for intracellular antioxidant defense induction
Article Snippet: .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membrane at 100V for 2h and membranes blocked with 5% skimmed milk in TBS-T. Membranes were incubated with polyclonal primary antibody against Keap1 (Santa Cruz: E-20 1:1000 dilution in 5% skimmed milk in TBS-T), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (Santa Cruz; 1:1000 dilution in 5% skimmed milk in TBS-T). .. Membranes were developed by chemiluminescence using SuperSignal West Dura substrate (Pierce, Rockford, IL) and sequential images taken with quantitation only performed on bands which had not reached saturation as described in [ ].

Article Title: Modulation of mammary cancer cell migration by 15-deoxy-?12,14-prostaglandin J2: implications for anti-metastatic therapy
Article Snippet: .. Proteins were separated by SDS-PAGE, and transferred to nitrocellulose membranes at 100V for 2 h and then membranes blocked with 5% skim milk in TBS-T. Membranes were incubated with a polyclonal primary antibody against Keap1 (E-20 1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), followed by a HRP (horseradish peroxidase)-conjugated donkey anti-goat secondary antibody (1:1000 dilution in 5% skim milk in TBS-T; Santa Cruz). .. Membranes were developed by chemiluminescence using SuperSignal West Dura substrate (Pierce) and sequential images taken with quantitation only performed on bands which had not reached saturation.

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    Santa Cruz Biotechnology polyclonal primary antibody against keap1
    Polyclonal Primary Antibody Against Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal primary antibody against keap1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polyclonal primary antibody against keap1 - by Bioz Stars, 2020-04
    86/100 stars
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