polyclonal pax8 mouse antibody  (Santa Cruz Biotechnology)

Journal: The Journal of pathology

Article Title: Lineage tracing suggests that ovarian endosalpingiosis does not result from escape of oviductal epithelium

doi: 10.1002/path.5308

Figure Lengend Snippet: Ovarian endosalpingiosis lesions (indicated by black stars), are usually located in the medulla near ovarian hilum (A), but are occasionally found in the ovarian cortex (B). Some ovaries harbor more than one lesion (C). The epithelium lining endosalpingiotic glands and cysts is OVGP1 positive (inset) and includes both secretory and ciliated cells (D), with ciliated cells expressing tubulin (E) and secretory cells preferentially expressing PAX8 (F, inset shows high-magnification). Note the internal positive control for PAX8 (normal oviduct) in lower left corner of panel F at black arrow. Black arrowheads indicate ciliated cells in panels D and E. Scale bars: 200 μm (panels A, B, F); 100 μm (panel C); 50 μm (panels D, E).

Article Snippet: Primary antibodies used were goat anti-mouse OVGP1 polyclonal antibody (1:500, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rabbit anti-mouse GFP monoclonal antibody – also detects YFP expression (1:500, Cell Signaling, Danvers, MA, USA), rabbit anti-mouse PAX8 polyclonal antibody (1:750, Novus Biologicals, Englewood, CO, USA), and mouse anti-mouse tubulin monoclonal antibody (1:1000, Sigma-Aldrich).

Techniques: Expressing, Positive Control

Journal: The Journal of pathology

Article Title: Lineage tracing suggests that ovarian endosalpingiosis does not result from escape of oviductal epithelium

doi: 10.1002/path.5308

Figure Lengend Snippet: Although extra-ovarian rete also forms glands lined by columnar cells (A) that express PAX8 (C), the rete does not express OVGP1 (E). High magnifications of boxed areas in A, C, and E are shown in B, D, F, respectively. Note the internal positive control for detection of PAX8 and OVGP1 expression (normal oviduct at black arrows) in lower left corners of panels C and E. Scale bars: 200 μm (panels A, C, E); 50 μm (panels B, D, F).

Article Snippet: Primary antibodies used were goat anti-mouse OVGP1 polyclonal antibody (1:500, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rabbit anti-mouse GFP monoclonal antibody – also detects YFP expression (1:500, Cell Signaling, Danvers, MA, USA), rabbit anti-mouse PAX8 polyclonal antibody (1:750, Novus Biologicals, Englewood, CO, USA), and mouse anti-mouse tubulin monoclonal antibody (1:1000, Sigma-Aldrich).

Techniques: Positive Control, Expressing

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: Gene-gene interaction results for susceptibility to classic Papillary Thyroid Carcinoma.

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques:

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: Relative frequencies of the nine genotype combinations of the replicated interaction ( PAX8 - STK17B ) are shown for cases and controls (red and blue columns, respectively). The cell containing the high-risk genotype combination is highlighted in light red, those with low-risk combinations in light blue, and those with neutral combinations are uncoloured. Figure1a - based on the discovery stage (series I); - based on the replication stage (series II and III); – based on both stages combined (series I, II and III).

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques:

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: PCCl3 cells were transiently (A) or stably (B) silenced for the transcription factor Pax8 (siPax8). As a control, wild type or siScramble transfected cells were used. The expression levels were assessed by means of qRT-PCR (A, upper panel) or western blot (A, lower panel, and B).

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques: Stable Transfection, Transfection, Expressing, Quantitative RT-PCR, Western Blot

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: Gene-gene interaction results for susceptibility to classic Papillary Thyroid Carcinoma.

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques:

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: Relative frequencies of the nine genotype combinations of the replicated interaction ( PAX8 - STK17B ) are shown for cases and controls (red and blue columns, respectively). The cell containing the high-risk genotype combination is highlighted in light red, those with low-risk combinations in light blue, and those with neutral combinations are uncoloured. Figure1a - based on the discovery stage (series I); - based on the replication stage (series II and III); – based on both stages combined (series I, II and III).

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques:

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: PCCl3 cells were transiently (A) or stably (B) silenced for the transcription factor Pax8 (siPax8). As a control, wild type or siScramble transfected cells were used. The expression levels were assessed by means of qRT-PCR (A, upper panel) or western blot (A, lower panel, and B).

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques: Stable Transfection, Transfection, Expressing, Quantitative RT-PCR, Western Blot