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Santa Cruz Biotechnology polyclonal keap1 e20 antibody
Polyclonal Keap1 E20 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal keap1 e20 antibody/product/Santa Cruz Biotechnology
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polyclonal keap1 e20 antibody - by Bioz Stars, 2020-04
85/100 stars

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monoclonal m2 antibody

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Centrifugation:

Article Title: Fetal Alz-50 Clone 1 Interacts with the Human Orthologue of the Kelch-like Ech-Associated Protein †
Article Snippet: Cells were scraped in PBS and pelleted at 1000 rpm in a clinical centrifuge for 5 min. Pellets were resuspended in detergent lysis buffer [0.1% NP-40, 10 mM Tris (pH 8.0), 10 mM MgCl2 , 15 mM NaCl, 0.5 mM PMSF, 2 μ g/mL pepstatin A, and 1 μ g/mL leupeptin] and incubated on ice for 15 min. Debris was removed by centrifugation at 1400 g for 5 min. .. Monoclonal M2 antibody (Kodak, IBI) and the polyclonal Keap1 E20 antibody (Santacruz) were used at 1:1000 in TBS with 0.1% Tween 20 (TBST) overnight at 4 °C.

Transfection:

Article Title: Fetal Alz-50 Clone 1 Interacts with the Human Orthologue of the Kelch-like Ech-Associated Protein †
Article Snippet: Transfected PT67 cell protein extracts were prepared by detergent lysis. .. Monoclonal M2 antibody (Kodak, IBI) and the polyclonal Keap1 E20 antibody (Santacruz) were used at 1:1000 in TBS with 0.1% Tween 20 (TBST) overnight at 4 °C.

Electrophoresis:

Article Title: Fetal Alz-50 Clone 1 Interacts with the Human Orthologue of the Kelch-like Ech-Associated Protein †
Article Snippet: Equal amounts of protein were loaded onto a 4–12% NuPAGE gradient gel (Invitrogen) and fractionated by size via electrophoresis for 1 h at 100 V. The proteins were transferred from the NuPAGE gel to PVDF by electrophoresis and blocked in 2% bovine serum albumin in TBS [10 mM Tris (pH 8.0), 150 mM NaCl]. .. Monoclonal M2 antibody (Kodak, IBI) and the polyclonal Keap1 E20 antibody (Santacruz) were used at 1:1000 in TBS with 0.1% Tween 20 (TBST) overnight at 4 °C.

Incubation:

Article Title: Fetal Alz-50 Clone 1 Interacts with the Human Orthologue of the Kelch-like Ech-Associated Protein †
Article Snippet: Cells were scraped in PBS and pelleted at 1000 rpm in a clinical centrifuge for 5 min. Pellets were resuspended in detergent lysis buffer [0.1% NP-40, 10 mM Tris (pH 8.0), 10 mM MgCl2 , 15 mM NaCl, 0.5 mM PMSF, 2 μ g/mL pepstatin A, and 1 μ g/mL leupeptin] and incubated on ice for 15 min. Debris was removed by centrifugation at 1400 g for 5 min. .. Monoclonal M2 antibody (Kodak, IBI) and the polyclonal Keap1 E20 antibody (Santacruz) were used at 1:1000 in TBS with 0.1% Tween 20 (TBST) overnight at 4 °C.

Lysis:

Article Title: Fetal Alz-50 Clone 1 Interacts with the Human Orthologue of the Kelch-like Ech-Associated Protein †
Article Snippet: Cells were scraped in PBS and pelleted at 1000 rpm in a clinical centrifuge for 5 min. Pellets were resuspended in detergent lysis buffer [0.1% NP-40, 10 mM Tris (pH 8.0), 10 mM MgCl2 , 15 mM NaCl, 0.5 mM PMSF, 2 μ g/mL pepstatin A, and 1 μ g/mL leupeptin] and incubated on ice for 15 min. Debris was removed by centrifugation at 1400 g for 5 min. .. Monoclonal M2 antibody (Kodak, IBI) and the polyclonal Keap1 E20 antibody (Santacruz) were used at 1:1000 in TBS with 0.1% Tween 20 (TBST) overnight at 4 °C.

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    Santa Cruz Biotechnology goat anti keap1 polyclonal antibody e20
    Goat Anti Keap1 Polyclonal Antibody E20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti keap1 polyclonal antibody e20/product/Santa Cruz Biotechnology
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    goat anti keap1 polyclonal antibody e20 - by Bioz Stars, 2020-04
    86/100 stars
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