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Santa Cruz Biotechnology polyclonal antibody against mouse keap1
Polyclonal Antibody Against Mouse Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibody against mouse keap1/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polyclonal antibody against mouse keap1 - by Bioz Stars, 2020-03
94/100 stars

Related Products / Commonly Used Together

immunoprecipitation
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Related Articles

Co-Immunoprecipitation Assay:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: Co-immunoprecipitation Co-immunoprecipitation (Co-IP) was performed following our previously published methods [ ]. .. Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz).

Negative Control:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz). .. IgG was used as a negative control for precipitation.

Cell Culture:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: .. Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz). .. After 3 hr incubation, protein G sepharose was added and incubated overnight at 4 °C, and then centrifuged for 1 min at 12,000g .

Mouse Assay:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: .. Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz). .. After 3 hr incubation, protein G sepharose was added and incubated overnight at 4 °C, and then centrifuged for 1 min at 12,000g .

Immunoprecipitation:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: .. Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz). .. After 3 hr incubation, protein G sepharose was added and incubated overnight at 4 °C, and then centrifuged for 1 min at 12,000g .

Incubation:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz). .. After 3 hr incubation, protein G sepharose was added and incubated overnight at 4 °C, and then centrifuged for 1 min at 12,000g .

Binding Assay:

Article Title: HDAC3 inhibition prevents blood-brain barrier permeability through Nrf2 activation in type 2 diabetes male mice
Article Snippet: Proteins were extracted from mice at day 7 or cultured HBMEC, and immunoprecipitation was performed using 2 μg polyclonal antibody against mouse Keap1 (1:1,000, Cat Number: sc-33569, RRID: AB_2280949, Santa Cruz). .. The precipitates were rinsed with immunoprecipitation buffer (0.5% NP-40, Tris-Cl pH 8.0, 0.15 M NaCl) four times to remove non-specific binding molecules.

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    Santa Cruz Biotechnology polyclonal antibody against mouse keap1
    Polyclonal Antibody Against Mouse Keap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against mouse keap1/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody against mouse keap1 - by Bioz Stars, 2020-03
    94/100 stars
      Buy from Supplier

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