polyclonal rabbit anti human stmn1 ser38 antibody  (Cell Signaling Technology Inc)

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Sequences of shRNAs targeting STMN1.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Sequencing, shRNA

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Primer sequences of genes analyzed for reverse transcription-quantitative PCR.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques:

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Clinicopathological characteristics of the included cases.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Expressing

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: STMN1 expression differences and immunohistochemical staining for STMN1 and LVI. (A and B) STMN1 expression level was increased in HSCC tissues compared with normal hypopharyngeal tissues, and was further increased in HSCC tissues of patients with metastases. (C and D) STMN1 expression status in different HSCC samples (magnification, x200). (E and F) LVI statuses in different HSCC samples along with STMN1 expression statuses (magnification, x400). STMN1, stathmin1; LVI, lymphatic vessel invasion; HSCC, hypopharyngeal squamous cell carcinoma.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: The transfection efficacy evaluation of STMN1 knockdown and the results of cell functional experiments. (A and B) The relative mRNA and protein expression levels of STMN1 in different groups. All of them were evidently decreased after STMN1 knockdown with different shRNA sequences, compared with the NC shRNA group. (C-E) The OD 450 , the relative migratory ratio at 48 h, and the relative number of invasive cells were all significantly decreased after STMN1 knockdown with different shRNA sequences; all were compared with the NC shRNA group. The magnification of picture D and picture E were 100 times and 200 times, respectively. * P<0.05 and ** P<0.01. STMN1, stathmin1; shRNA, short hairpin RNA; NC, negative control.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Transfection, Knockdown, Functional Assay, Expressing, shRNA, Negative Control

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Bioinformatics analyses for target genes and pathways of STMN1. (A) STMN1 was evidently enriched in the HIF-1α pathway in head and neck squamous cell carcinoma. (B) Gene expression levels of HIF-1α pathway were different between cases with high expression of STMN1 and those with low expression of STMN1. (C and D) Target gene prediction analysis from website https://www.aclbi.com , which demonstrated that the expression level of MTA1 was significantly correlated with that of STMN1. STMN1, stathmin1; HIF-1α, hypoxia inducible factor-1alpha; MTA1, tumor metastasis-associated protein 1.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Gene Expression, Expressing

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: The mRNA and protein expression levels of the target genes of STMN1 in FaDu cells. (A-C) The relative mRNA and protein expression levels of HIF-1α were signifiicantly decreased after STMN1 knockdown with different shRNA sequences. (D) The relative expression level of VEGF-A, the downstream protein of HIF-1α, was significantly decreased after STMN1 knockdown. (E and F) Both relative mRNA and protein expression levels of MTA1 significantly decreased after STMN1 knockdown. All were compared with the NC shRNA group. * P<0.05, ** P<0.01 and *** P<0.001. STMN1, stathmin1; HIF-1α, hypoxia inducible factor-1alpha; shRNA, short hairpin RNA; VEGF, vascular endothelial growth factor; MTA1, tumor metastasis-associated protein 1; NC, negative control.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Expressing, Knockdown, shRNA, Negative Control

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 and its phosphorylation-related proteins and clinical outcomes for breast cancer patients. (A) Relationship between Kaplan-Meier analysis of DFS and STMN1 level by IHC (400×). (B) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser16 levelby IHC (400×). (C) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser25 levelby IHC (400×). (D) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser38 levelby IHC (400×). (E) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser63 levelby IHC (400×). (F) Relationship between Kaplan-Meier analysis of DFS and GRP78 level by IHC (400×). DFS, disease-free survival.

Article Snippet: Polyclonal rabbit anti-human STMN1 antibody (Proteintech) was diluted to 1:400 and incubated with samples in a humid chamber at 4 °C overnight.

Techniques: Phospho-proteomics

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 phosphorylation and pathologic response. (A) Correlation between pathologic response and STMN1 expression. (B) Correlation between pathologic response and STMN1 phosphorylation at Ser-16. (C) Correlation between pathologic response and STMN1 phosphorylation at Ser-25. (D) Correlation between pathologic response and STMN1 phosphorylation at Ser-38. (E) Correlation between pathologic response and STMN1 phosphorylation at Ser-63. (F) Correlation between pathologic response and GRP78. pCR, pathological complete response.

Article Snippet: Polyclonal rabbit anti-human STMN1 antibody (Proteintech) was diluted to 1:400 and incubated with samples in a humid chamber at 4 °C overnight.

Techniques: Phospho-proteomics, Expressing

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: p-STMN1/GRP78 model powerfully predicts DFS for patients treated with NACT. (A) Kaplan-Meier analysis of DFS in breast cancer patients from high and low-risk groups classified by the p-STMN1/GRP78 model. (B) ROC curves to test the prognostic accuracy of the p-STMN1/GRP78 model. DFS, disease-free survival; ROC, receiver operating characteristic; AUC, area under the receiver operating characteristic curve.

Article Snippet: Polyclonal rabbit anti-human STMN1 antibody (Proteintech) was diluted to 1:400 and incubated with samples in a humid chamber at 4 °C overnight.

Techniques:

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 and its phosphorylation-related proteins and clinical outcomes for breast cancer patients. (A) Relationship between Kaplan-Meier analysis of DFS and STMN1 level by IHC (400×). (B) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser16 levelby IHC (400×). (C) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser25 levelby IHC (400×). (D) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser38 levelby IHC (400×). (E) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser63 levelby IHC (400×). (F) Relationship between Kaplan-Meier analysis of DFS and GRP78 level by IHC (400×). DFS, disease-free survival.

Article Snippet: Polyclonal rabbit anti-human STMN1 Ser38 antibody (Cell Signaling Technologies) was diluted to 1:100 and incubated with samples in a humid chamber at 4 °C overnight. respectively.

Techniques: Phospho-proteomics

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 phosphorylation and pathologic response. (A) Correlation between pathologic response and STMN1 expression. (B) Correlation between pathologic response and STMN1 phosphorylation at Ser-16. (C) Correlation between pathologic response and STMN1 phosphorylation at Ser-25. (D) Correlation between pathologic response and STMN1 phosphorylation at Ser-38. (E) Correlation between pathologic response and STMN1 phosphorylation at Ser-63. (F) Correlation between pathologic response and GRP78. pCR, pathological complete response.

Article Snippet: Polyclonal rabbit anti-human STMN1 Ser38 antibody (Cell Signaling Technologies) was diluted to 1:100 and incubated with samples in a humid chamber at 4 °C overnight. respectively.

Techniques: Phospho-proteomics, Expressing

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: p-STMN1/GRP78 model powerfully predicts DFS for patients treated with NACT. (A) Kaplan-Meier analysis of DFS in breast cancer patients from high and low-risk groups classified by the p-STMN1/GRP78 model. (B) ROC curves to test the prognostic accuracy of the p-STMN1/GRP78 model. DFS, disease-free survival; ROC, receiver operating characteristic; AUC, area under the receiver operating characteristic curve.

Article Snippet: Polyclonal rabbit anti-human STMN1 Ser38 antibody (Cell Signaling Technologies) was diluted to 1:100 and incubated with samples in a humid chamber at 4 °C overnight. respectively.

Techniques:

Journal: Cell Reports

Article Title: Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals

doi: 10.1016/j.celrep.2022.110610

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-STMN1 , Proteintech , Cat# 11157-1-AP, RRID:AB_2197114.

Techniques: Recombinant, Software