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rabbit polyclonal anti sars cov 2 orf8  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal anti sars cov 2 orf8
    (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or <t>ORF8</t> in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).
    Rabbit Polyclonal Anti Sars Cov 2 Orf8, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti sars cov 2 orf8/product/Novus Biologicals
    Average 94 stars, based on 4 article reviews
    rabbit polyclonal anti sars cov 2 orf8 - by Bioz Stars, 2026-05
    94/100 stars

    Images

    1) Product Images from "One and Done: A safe, adaptable single-cycle SARS-CoV-2 vaccine platform blocks XBB.1.5 infection and transmission"

    Article Title: One and Done: A safe, adaptable single-cycle SARS-CoV-2 vaccine platform blocks XBB.1.5 infection and transmission

    Journal: bioRxiv

    doi: 10.64898/2026.03.09.709481

    (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or ORF8 in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).
    Figure Legend Snippet: (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or ORF8 in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).

    Techniques Used: Virus, Expressing, Staining, Infection, Western Blot, Control



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    (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or <t>ORF8</t> in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).
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    (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or <t>ORF8</t> in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).
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    (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or <t>ORF8</t> in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).
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    Image Search Results


    (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or ORF8 in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).

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    Article Title: One and Done: A safe, adaptable single-cycle SARS-CoV-2 vaccine platform blocks XBB.1.5 infection and transmission

    doi: 10.64898/2026.03.09.709481

    Figure Lengend Snippet: (a) Schematic illustration of the SARS-CoV-2 genomic landscape and the deletions/substitutions in ΔE673* XBB (scVac XBB ); position of main structural and accessory proteins as indicated. Positions of the overlapping PCR fragments (A-D2) covering the whole SARS-CoV-2 genome are indicated. (b) Cell-free virus after passage of either wild-type SARS-CoV-2 expressing an Omicron XBB.1.5 Spike (rCoV2 XBB ) or scVac XBB after 0-3 virus passages is shown for three non-complementing cell lines as indicated. The level of input virus is indicated by a dotted line. (c) Staining of N, S, and ORF7a (magenta), F-actin (green), nuclei (blue), and ORF6, ORF3a, or ORF8 in Vero E6-TMPRSS2 cells infected with rCoV2 XBB and scVac XBB . (d) Immunoblot analysis of viral proteins 24h after infection of Vero E6-TMPRSS2 cells with the indicated viruses or medium only (control), probed with anti-NSP2, anti-N, anti-S, anti-ORF3a (full-length [fl] and truncated [tr] forms indicated with arrows), anti-ORF6, anti-ORF7a, and anti-beta-actin (β-ACT) antibodies. Scale bar is 50 µm and 20 µm in (c) (overview and ROI images, respectively).

    Article Snippet: The following antibodies were used for immunocytochemistry and immunoblotting: mouse monoclonal anti-β-actin (Cell Signaling Technology; 3700; RRID: AB_2242334; LOT# 20), rabbit polyclonal anti-SARS-CoV-2 nsp2 (GeneTex; GTX135717; RRID: AB_2909866; LOT# B318853), mouse monoclonal anti-SARS-CoV-2 Nucleocapsid protein (4F3C4, gift from S. Reiche [ ], sheep polyclonal anti-SARS-CoV-2 ORF3a [ ], rat monoclonal anti-SARS-CoV-2 ORF6 (8B10, gift from Y. Miyamoto [ ]), mouse monoclonal anti-SARS-CoV-2 ORF7a (3C9; GeneTex; GTX632602; RRID: AB_2888320; LOT# 42219), rabbit polyclonal anti-SARS-CoV-2 ORF8 (Novus Biologicals; NBP3-07972; LOT# 25966-2102), mouse monoclonal anti-SARS-CoV-2 Spike protein (4B5C1, gift from S. Reiche).

    Techniques: Virus, Expressing, Staining, Infection, Western Blot, Control