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Illumina Inc poly t oligo attached magnetic beads
Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 41 article reviews
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poly t oligo attached magnetic beads - by Bioz Stars, 2020-01
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Amplification:

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt). .. After that, the libraries were end-repaired and ligated with adaptors, and amplified with Phusion DNA polymerase.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. After end repair process, cDNA fragments were linked with adaptors and amplified by PCR.

Sample Prep:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s recommendations. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Sequencing libraries were generated using an Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). .. Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II.

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: Briefly, the cDNA libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) following the manufacturer's recommendations. .. Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: The cDNA library for Illumina sequencing was prepared by using a TruseqTM RNA sample prep kit (Illumina). .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: The stranded total RNA libraries were prepared in accordance to the Illumina TruSeq Stranded mRNA Sample Preparation (October 2013 Rev E.) for Illumina Paired-End Multiplexed Sequencing. .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification.

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: Briefly, the cDNA libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) following the manufacturer’s protocol. .. Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: 2.3. cDNA Library Construction and RNA-Seq Libraries (cDNA) of high quality RNA samples (RIN (RNA Integrity Number) > 7) were prepared using the Illumina® TruSeqTM RNA Sample Preparation kit (Cat. No. 15008136 A, Illumina Inc. CA, USA) following the manufacturer’s instructions. .. The mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Cat. No. MRZ11124C, Illumina Inc. CA, USA).

Magnetic Beads:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). ..

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: .. Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II. .. Second-strand cDNA was then synthesized.

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: .. Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer. .. First- and second-strand cDNAs were synthesized using random oligonucleotides and SuperScript II followed by DNA polymerase I and RNase H treatment.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt). .. The first and second strands of cDNA were synthesized by SuperScript II reverse transcriptase and DNA polymerase I, respectively.

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: .. The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ). .. The poly(A) RNA was then fragmented into smaller pieces using divalent cations at 94 °C for 4 min. First-strand cDNA was synthesized using SuperScript II reverse transcriptase and random primers.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: .. For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. The purified mRNA was cut into fragments with an average length of 155 bp using divalent cations at 95°C for 8 min.

Article Title: Transcriptomic analysis reveals vacuolar Na+ (K+)/H+ antiporter gene contributing to growth, development, and defense in switchgrass (Panicum virgatum L.)
Article Snippet: .. Briefly, poly (A)-containing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. Then, the mRNA was broken into short fragments by a fragmentation buffer (Ambion, Austin, TX, USA); the fragments were used as templates for cDNA synthesis.

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification. ..

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: .. Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer. .. First and second strand cDNAs were synthesized using random oligonucleotides and SuperScript II, followed by DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase and, after 3 ’ end adenylation, Illumina PE adapter oligonucleotides were ligated.

Isolation:

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II.

Article Title: Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, total RNA from FW PVCs, FW MRCs, SW PVCs and SW MRCs (each n = 2) was extracted using the mirVanaTM miRNA isolation kit (Applied Biosystems). .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: Paragraph title: RNA Isolation, cDNA Library Construction, and Illumina Deep Sequencing ... Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

Biomarker Assay:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Library construction and RNA-Seq Construction of the four libraries and the RNA-Seq were performed by the Biomarker Biotechnology Corporation (Beijing, China). .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Synthesized:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. Double-stranded cDNA was synthesized using SuperScript II, buffer, dNTPs, RNaseH, DNA polymerase I, and random hexamer primers (Illumina).

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: .. Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II. .. Second-strand cDNA was then synthesized.

Article Title: Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer. .. First- and second-strand cDNAs were synthesized using random oligonucleotides and SuperScript II followed by DNA polymerase I and RNase H treatment.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt). .. The first and second strands of cDNA were synthesized by SuperScript II reverse transcriptase and DNA polymerase I, respectively.

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ). .. The poly(A) RNA was then fragmented into smaller pieces using divalent cations at 94 °C for 4 min. First-strand cDNA was synthesized using SuperScript II reverse transcriptase and random primers.

Article Title: Transcriptomic analysis reveals vacuolar Na+ (K+)/H+ antiporter gene contributing to growth, development, and defense in switchgrass (Panicum virgatum L.)
Article Snippet: Briefly, poly (A)-containing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. First-strand cDNA was synthesized using random hexamer-primers and M-MuLV reverse transcriptase (RNase H).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer. .. First and second strand cDNAs were synthesized using random oligonucleotides and SuperScript II, followed by DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase and, after 3 ’ end adenylation, Illumina PE adapter oligonucleotides were ligated.

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: The mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Cat. No. MRZ11124C, Illumina Inc. CA, USA). .. The first strand cDNA was synthesized using random oligonucleotide primers and SuperScriptTM II reverse transcriptase (Cat. No. 18064014, Invitrogen, CA, USA), while the second strand cDNA synthesis was undertaken using DNA polymerase I.

RNA Sequencing Assay:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Paragraph title: Library construction and RNA-Seq ... Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Paragraph title: Library construction and Illumina RNA-seq ... Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: Paragraph title: Library Construction and RNA-seq ... Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: Paragraph title: 2.3. cDNA Library Construction and RNA-Seq ... The mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Cat. No. MRZ11124C, Illumina Inc. CA, USA).

Ligation:

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II. .. These cDNA fragments were then subjected to an end repair process, and to adenylation and ligation of adapters P5 and P7.

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer. .. Overhangs were blunted by treatment with exonuclease/polymerase followed by 3′-end adenylation and ligation to Illumina PE adaptor oligonucleotides.

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification. .. A single “A” nucleotide is added to the 3′ end of the blunt fragments to prevent ligating to one another during adapter ligation reaction.

Construct:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Five micrograms of total RNA from each sample (EM, EH, LM, and LH) were used to construct the sequencing libraries. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: cDNA library establishment and sequencing The cDNA library was constructed using the NEBNext Ultra RNA Library Prep Kit (NEB, Ipswich, USA) according to the manufacturer’s instructions [ ]. .. The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ).

Purification:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). ..

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: .. Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II. .. Second-strand cDNA was then synthesized.

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: .. Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer. .. First- and second-strand cDNAs were synthesized using random oligonucleotides and SuperScript II followed by DNA polymerase I and RNase H treatment.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: .. For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. The purified mRNA was cut into fragments with an average length of 155 bp using divalent cations at 95°C for 8 min.

Article Title: Transcriptomic analysis reveals vacuolar Na+ (K+)/H+ antiporter gene contributing to growth, development, and defense in switchgrass (Panicum virgatum L.)
Article Snippet: .. Briefly, poly (A)-containing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. Then, the mRNA was broken into short fragments by a fragmentation buffer (Ambion, Austin, TX, USA); the fragments were used as templates for cDNA synthesis.

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification. ..

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: .. Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer. .. First and second strand cDNAs were synthesized using random oligonucleotides and SuperScript II, followed by DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase and, after 3 ’ end adenylation, Illumina PE adapter oligonucleotides were ligated.

Sequencing:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Five micrograms of total RNA from each sample (EM, EH, LM, and LH) were used to construct the sequencing libraries. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Sequencing libraries were generated using an Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). .. Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II.

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: Paragraph title: Whole-testis transcriptome sequencing ... Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: The cDNA library for Illumina sequencing was prepared by using a TruseqTM RNA sample prep kit (Illumina). .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: Paragraph title: cDNA library establishment and sequencing ... The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ).

Article Title: Transcriptomic analysis reveals vacuolar Na+ (K+)/H+ antiporter gene contributing to growth, development, and defense in switchgrass (Panicum virgatum L.)
Article Snippet: Paragraph title: Library construction and sequencing ... Briefly, poly (A)-containing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: The stranded total RNA libraries were prepared in accordance to the Illumina TruSeq Stranded mRNA Sample Preparation (October 2013 Rev E.) for Illumina Paired-End Multiplexed Sequencing. .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification.

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: Paragraph title: RNA Isolation, cDNA Library Construction, and Illumina Deep Sequencing ... Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

Polymerase Chain Reaction:

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II. .. The insert size of the library was restricted to approximately 300 bp using PCR products purified with the AMPure XP system (Beckman Coulter, Beverly, MA, USA).

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: Index codes were ligated to identify the individual samples. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) before fragmentation using divalent cations under elevated temperature in Illumina fragmentation buffer. .. DNA fragments successfully ligated with adaptor molecules on both ends were enriched using the Illumina PCR Primer Cocktail in a 15 cycle PCR reaction.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. DNA fragments that ligated with adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycles PCR reaction.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. After end repair process, cDNA fragments were linked with adaptors and amplified by PCR.

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer. .. DNA fragments that ligated with adaptor molecules on both ends were enriched using the Illumina PCR Primer Cocktail in a 15-cycle PCR reaction.

Random Hexamer Labeling:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. Double-stranded cDNA was synthesized using SuperScript II, buffer, dNTPs, RNaseH, DNA polymerase I, and random hexamer primers (Illumina).

Article Title: Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

cDNA Library Assay:

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II.

Article Title: Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: The cDNA library for Illumina sequencing was prepared by using a TruseqTM RNA sample prep kit (Illumina). .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: Paragraph title: cDNA library establishment and sequencing ... The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ).

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: Paragraph title: RNA Isolation, cDNA Library Construction, and Illumina Deep Sequencing ... Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: Paragraph title: 2.3. cDNA Library Construction and RNA-Seq ... The mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Cat. No. MRZ11124C, Illumina Inc. CA, USA).

Agarose Gel Electrophoresis:

Article Title: Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data
Article Snippet: The RNA quality was verified using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and confirmed using RNase-free agarose gel electrophoresis. .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Fluorescence In Situ Hybridization:

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: RNA Isolation, cDNA Library Construction, and Illumina Deep Sequencing Total RNA was isolated from the CS glands of fish using TRIzol reagent (Life Technologies, CA, USA). .. Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

Concentration Assay:

Article Title: Development of Gene-Based SSR Markers in Rice Bean (Vigna umbellata L.) Based on Transcriptome Data
Article Snippet: The concentration of the total RNA was further quantified using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, Delaware USA). .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Transcriptomic responses of corpuscle of Stannius gland of Japanese eels (Anguilla japonica) to Changes in Water Salinity
Article Snippet: The RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). .. Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and, then, fragmented using divalent cations under elevated temperature in the Illumina fragmentation buffer.

Generated:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s recommendations. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptome Profiling of Resistance to Fusarium oxysporum f. sp. conglutinans in Cabbage (Brassica oleracea) Roots
Article Snippet: Sequencing libraries were generated using an Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). .. Briefly, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads and fragmented using Illumina proprietary fragmentation buffer at 94°C for 15 min. First-strand cDNA was synthesized using a 6 bp-random primer and SuperScript II.

Article Title: Transcriptomic analysis reveals vacuolar Na+ (K+)/H+ antiporter gene contributing to growth, development, and defense in switchgrass (Panicum virgatum L.)
Article Snippet: Libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s instructions. .. Briefly, poly (A)-containing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

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    Illumina Inc poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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