poly t oligos beads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligos beads
    Poly T Oligos Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligos beads/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    poly t oligos beads - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    ribosomal rna
    rna

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    RNA Extraction:

    Article Title: Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia
    Article Snippet: RNA sequencing Tissue samples were immediately placed in RNAlater® (Ambion, Life Technologies, USA), and after 48 h at +4 °C were stored at −80 °C until RNA extraction. .. Library preparation and sequencing has been described previously [ ], but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen).

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: Tissue samples were immediately placed in RNAlater® (Ambion, ThermoFisher Scientific, Waltham, MA), and after 48 h at +4°C were stored at −80°C until RNA extraction. .. Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY).

    RNA Sequencing Assay:

    Article Title: Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia
    Article Snippet: Paragraph title: RNA sequencing ... Library preparation and sequencing has been described previously [ ], but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen).

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: Paragraph title: RNA sequencing ... Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY).

    Isolation:

    Article Title: Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia
    Article Snippet: Total RNA was isolated with Trizol and RNA quality was assessed using the Agilent Bioanalyzer 2100 (Agilent) with no samples being excluded for poor quality. .. Library preparation and sequencing has been described previously [ ], but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen).

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: Total RNA was isolated with Trizol and RNA quality was assessed using the Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA) with no samples being excluded for poor quality. .. Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY).

    Purification:

    Article Title: Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia
    Article Snippet: Library preparation and sequencing has been described previously [ ], but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen) and resolved with EB buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY). .. Double-stranded DNA synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with elution buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Sequencing:

    Article Title: Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia
    Article Snippet: .. Library preparation and sequencing has been described previously [ ], but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen). .. RNAs were reverse transcribed using random hexamers (Invitrogen).

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: .. Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY). .. RNAs were reverse transcribed using random hexamers (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia
    Article Snippet: Library preparation and sequencing has been described previously [ ], but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen) and resolved with EB buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY). .. Double-stranded DNA synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with elution buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    DNA Synthesis:

    Article Title: Using Next-generation RNA Sequencing to Examine Ischemic Changes Induced by Cold Blood Cardioplegia on the Human Left Ventricular Myocardium Transcriptome
    Article Snippet: Library preparation and sequencing has been described previously (see ), but briefly, ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligos beads (Invitrogen, Life Technologies, Grand Island, NY). .. Double-stranded DNA synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with elution buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

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    Thermo Fisher oligo
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    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher poly t oligos
    Poly T Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligos/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
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