poly t oligo  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligo
    Poly T Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo/product/Thermo Fisher
    Average 98 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    poly t oligo - by Bioz Stars, 2020-04
    98/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Amplification:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Synthesized:

    Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues
    Article Snippet: .. RNA was extracted from the tissues using Trizol (Invitrogen, Carisbad, CA) and first strand cDNA was synthesized using a poly-T oligo and the superscript kit (Invitrogen, Carisbad, CA). .. To select which SNPs to genotype in our samples (see Fig. ), we used a heuristic that prioritized SNPs based on two criteria: (i) The ability of the genotype at a SNP to significantly explain expression in at least two of the three HapMap populations using simple additive linear models and (ii) a minor allele frequency ≥0.2 in the CEU HapMap population.

    Construct:

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: mRNA library construction and sequencing Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Then the cleaved RNA fragments were constructed into the final cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher). .. Then the cleaved RNA fragments were constructed into the final cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Incubation:

    Article Title: m6A Methylation Analysis of Differentially Expressed Genes in Skin Tissues of Coarse and Fine Type Liaoning Cashmere Goats
    Article Snippet: Approximately more than 200ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were subjected to incubated for 2h at 4°C; with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630) supplemented with BSA (0.5 μg/ μl).

    Expressing:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Paragraph title: 4.4. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System ... Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols.

    Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues
    Article Snippet: Given we are primarily interested in the effect of cis regulatory variation, and not the genetic basis of expression covariation among tissues, having largely non-overlapping sets of individuals is preferable to having all tissues from each individual because non-overlapping sets will reduce the impact of individual-specific effects, such as environmental effects, which otherwise could confound the analysis. .. RNA was extracted from the tissues using Trizol (Invitrogen, Carisbad, CA) and first strand cDNA was synthesized using a poly-T oligo and the superscript kit (Invitrogen, Carisbad, CA).

    Ligation:

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: mRNA library construction and sequencing Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Then the cleaved RNA fragments were constructed into the final cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher). .. Then the cleaved RNA fragments were constructed into the final cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Generated:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    DNA Sequencing:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The cloned IPD083Aa PCR product was confirmed by DNA sequencing.

    Sequencing:

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: .. mRNA library construction and sequencing Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Paragraph title: Construction of mRNA library and sequencing ... Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: .. Construction and Sequencing of the mRNA Library Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Following purification, the mRNA was fragmented into small pieces using divalent cations at elevated temperatures.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. In the single-end libraries, the average insert size was 180bp–200 bp.Then following the vendor's recommended protocol, we operated single-end sequencing (50 bp) on the Illumina Hiseq2500.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. Finally, the paired-end sequencing was performed on an Illumina Hiseq2000/2500.

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: The quality and quantity of both DNA and RNA were checked by gel electrophoresis and the DW-K5500 spectrophotometer (DRAWELL). .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    RNA Sequencing Assay:

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Paragraph title: RNA sequencing and RNA-Seq data analysis ... Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: For RNA-Seq library preparation, total RNA was extracted from longissimus dorsi using TRIzol (Invitrogen, CA, USA) and further purified with RNeasy column (Qiagen, USA) according to the manufacturer's protocol. .. The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher).

    Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
    Article Snippet: Paragraph title: RNA sequencing and RNA-Seq data analysis ... Roughly 10 µg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen; Thermo Fisher Scientific, Inc.).

    Magnetic Beads:

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: .. mRNA library construction and sequencing Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: .. Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: .. Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: .. Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: .. Construction and Sequencing of the mRNA Library Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Following purification, the mRNA was fragmented into small pieces using divalent cations at elevated temperatures.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. After purification, by use of divalent cations through elevated temperature, the mRNA was split into small fragments.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: .. Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. The purified mRNA was fragmented into small pieces by divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: .. The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
    Article Snippet: .. Roughly 10 µg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen; Thermo Fisher Scientific, Inc.). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Isolation:

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: Paragraph title: RNA isolation, preparation of library for DGE ... By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: .. Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. The purified mRNA was fragmented into small pieces by divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: .. The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    RNA Extraction:

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Paragraph title: Total RNA extraction and building of DNA library ... Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen).

    Purification:

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: mRNA library construction and sequencing Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Construction and Sequencing of the mRNA Library Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Following purification, the mRNA was fragmented into small pieces using divalent cations at elevated temperatures.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: RNA isolation, preparation of library for DGE Following the manufacturer's protocol, total RNA of each sample was extracted by use of RNAiso Plus reagent (TaKaRa), then purified with absolute alcohol and treated with DNase I. .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. The purified mRNA was fragmented into small pieces by divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: For RNA-Seq library preparation, total RNA was extracted from longissimus dorsi using TRIzol (Invitrogen, CA, USA) and further purified with RNeasy column (Qiagen, USA) according to the manufacturer's protocol. .. The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher).

    Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
    Article Snippet: Roughly 10 µg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen; Thermo Fisher Scientific, Inc.). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Polymerase Chain Reaction:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher). .. A reverse transcription followed by PCR was used to create cDNA constructs.

    cDNA Library Assay:

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: mRNA library construction and sequencing Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Then the cleaved RNA fragments were constructed into the final cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, CA, USA).

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Construction and Sequencing of the mRNA Library Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. According to the manufacturer's protocol of the mRNA-Seq sample preparation kit (Illumina), the RNA fragments were reverse-transcribed which were then used to create the cDNA library.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. Then the final cDNA library was produced by the reverse-transcription of the cleaved RNA fragments on the basis of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), and the average 300±50 bp insert size was used for the paired-end libraries.

    Article Title: Transcriptome analysis of genetic mechanism of growth curve inflection point using a pig model
    Article Snippet: The total RNA was isolated poly (A) mRNA by poly-T oligo attached magnetic beads (Thermo-Fisher). .. Then the cleaved RNA fragments were constructed into the final cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
    Article Snippet: Roughly 10 µg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen; Thermo Fisher Scientific, Inc.). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, Inc., San Diego, CA, USA).

    Chromatin Immunoprecipitation:

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: The total RNA quantity and purity were analyzed with Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agi-lent, CA, USA) with RIN number > 7.0. .. Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Plasmid Preparation:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The PCR product was then sub-cloned into a plant transient vector containing the DMMV promoter and a transcriptional terminator with a kanamycin selectable marker (NPTIII) [ ].

    Functional Assay:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Sample Prep:

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, CA, USA).

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Construction and Sequencing of the mRNA Library Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. According to the manufacturer's protocol of the mRNA-Seq sample preparation kit (Illumina), the RNA fragments were reverse-transcribed which were then used to create the cDNA library.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. Then the final cDNA library was produced by the reverse-transcription of the cleaved RNA fragments on the basis of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), and the average 300±50 bp insert size was used for the paired-end libraries.

    Spectrophotometry:

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: The quality and quantity of both DNA and RNA were checked by gel electrophoresis and the DW-K5500 spectrophotometer (DRAWELL). .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    Produced:

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. Then the final cDNA library was produced by the reverse-transcription of the cleaved RNA fragments on the basis of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), and the average 300±50 bp insert size was used for the paired-end libraries.

    Marker:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The PCR product was then sub-cloned into a plant transient vector containing the DMMV promoter and a transcriptional terminator with a kanamycin selectable marker (NPTIII) [ ].

    Fluorescence In Situ Hybridization:

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: We performed simple anatomy of the whole fish, i.e., removing gill, fin and tail tissue, while retaining visceral mass, head, spine and other nerve systems. .. Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen).

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  • 93
    Thermo Fisher poly ra oligo dt assays
    Poly Ra Oligo Dt Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher poly t oligo
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    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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