poly t oligo  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligo
    Poly T Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    poly t oligo - by Bioz Stars, 2019-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The libraries were generated by PCR after selection and purification of the cDNA fragments.

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The second-strand cDNA was end-repaired using End Repair Mix (Illumina), and a single A base was added at the 3ʹ end for adapter ligation.

    Construct:

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Two mRNA libraries were constructed for each group (H and L) from total RNAs pooled from two individuals. .. Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. The cleaved RNA fragments were then reverse transcribed into the final complementary DNA (cDNA) library in accordance with the Illumina RNA ligation-based method (Illumina, San Diego, CA, United States).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the sRNA-seq experiment, approximately 1 μg of total RNA was used to construct an sRNA library, according to the protocol of the TruSeq™ Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Ligation:

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. Then, first-strand complementary DNA (cDNA) was synthesized using reverse transcriptase and random primers, followed by synthesis of second-strand cDNA.

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Bridge PCR:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The cDNA target fragments were selected on 2% Low Range Ultra Agarose (Bio-Red), followed by 15 cycles of PCR amplification.

    Generated:

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA).

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. The constructed RNA libraries were sequenced using an Illumina HiSeq 2000 platform.

    Sequencing:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: Paragraph title: 2.2. RNA Isolation, Library Construction and Sequencing ... Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats
    Article Snippet: A sub-fraction of isolated RNA (ERS n = 8, ERD n = 8; four males and four females per treatment) was shipped on dry ice for commercial mRNA and miRNA sequencing (LC Sciences, Houston, TX, United States). .. Total RNA was subjected to poly(A) mRNA enrichment with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, United States).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Paragraph title: mRNA sequencing and data analysis ... Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. According to the manufacturer's protocol of the mRNA-Seq sample preparation kit (Illumina), the RNA fragments were reverse-transcribed which were then used to create the cDNA library.

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: Paragraph title: 4.4. Construction of the mRNA Libraries and High-Throughput Sequencing ... 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: Paragraph title: mRNA library construction and sequencing ... The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: Paragraph title: Messenger RNA (mRNA) Library Construction and Sequencing ... Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher).

    Article Title: Digital Gene Expression Analysis of Microsporum canis Exposed to Berberine Chloride
    Article Snippet: Approximately 10 μg of total RNA from each sample was subjected to isolate Poly (A) mRNA by using poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the single-end libraries was 200bp-350bp.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: Single-end sequencing (50 bp) was performed on the Illumina Hiseq. .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Paragraph title: Construction of mRNA library and sequencing ... Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The cDNA target fragments were selected on 2% Low Range Ultra Agarose (Bio-Red), followed by 15 cycles of PCR amplification.

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Paragraph title: Construction and Sequencing of the mRNA Library ... Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China).

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Paragraph title: 2.2. mRNA Library Construction and Sequencing ... Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Paragraph title: Library Construction and Sequencing ... Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: Paragraph title: mRNA library construction and sequencing ... Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher).

    Binding Assay:

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: First, only RNA samples that had RNA Integrity Number (RIN) scores > 8 were used for sequencing. .. Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Nucleic Acid Electrophoresis:

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: The quality and quantity of both DNA and RNA were checked by gel electrophoresis and the DW-K5500 spectrophotometer (DRAWELL). .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    RNA Sequencing Assay:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: To simplify the description, we designated three replicates of the samples for RNA sequencing corded, as follows; for G. raimondii , root samples (Gr_0R, Gr_12R, Gr_48R), stem (Gr_0S, Gr_12S, Gr_48S), and the leaf (Gr_0L, Gr_12L, Gr_48L), G. thurberi root (Gth_0R, Gth_12R, Gth_48R), stem (Gth_0S, Gth_12S, Gth_48S), and the leaf (Gth_0L, Gth_12L, Gth_48L); and finally, for the G. trilobum root (Gtr_0R, Gtr_12R, Gtr_48R), stem (Gtr_0S, Gtr_12S, Gtr_48S), and the leaf (Gtr_0L, Gtr_12L, Gtr_48L). .. Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Paragraph title: RNA sequencing and RNA-Seq data analysis ... Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: Single-end sequencing (50 bp) was performed on the Illumina Hiseq. .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA). .. Following purification, the mRNAs were fragmented into small pieces using divalent cations at an elevated temperature.

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: Paragraph title: RNA extraction, transcriptome library preparation, and RNA-Seq ... PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer.

    Magnetic Beads:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) and RNA 6000 Nano Lab Chip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: The total RNA quantity and purity were analyzed with Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agi-lent, CA, USA) with RIN number > 7.0. .. Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Two mRNA libraries were constructed for each group (H and L) from total RNAs pooled from two individuals. .. Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the purified mRNA was randomly fragmented using divalent cations under elevated temperature.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: The quality and quantity of both DNA and RNA were checked by gel electrophoresis and the DW-K5500 spectrophotometer (DRAWELL). .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. After purification, by use of divalent cations through elevated temperature, the mRNA was split into small fragments.

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: The total RNA quantity and purity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano Labchip Kit (Agilent, Carlsbad, CA, USA) with RIN number > 7. .. 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. The mRNA is fragmented into small pieces using a divalent cation under elevated temperature after purification, then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: A total amount of 3 μg of RNA per sample was used as the input material for the RNA sample preparations. .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. Fragmentation was carried out using divalent cations at an elevated temperature in NEB First Strand Synthesis reaction buffer (5×) (NEB, USA).

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano Lab-Chip Kit (Agilent, Santa Clara, CA, USA). .. Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: First, only RNA samples that had RNA Integrity Number (RIN) scores > 8 were used for sequencing. .. Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Digital Gene Expression Analysis of Microsporum canis Exposed to Berberine Chloride
    Article Snippet: Total RNA quantity and purity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 8.0. .. Approximately 10 μg of total RNA from each sample was subjected to isolate Poly (A) mRNA by using poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: Single-end sequencing (50 bp) was performed on the Illumina Hiseq. .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA). .. Following purification, the mRNAs were fragmented into small pieces using divalent cations at an elevated temperature.

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Their quantity and purity were analyzed by the Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity > 7.0. .. Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: The RNA-Seq transcriptome library was prepared using a Truseq RNA Sample Prep Kit (Illumina). .. PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. Then, first-strand complementary DNA (cDNA) was synthesized using reverse transcriptase and random primers, followed by synthesis of second-strand cDNA.

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: RNA contamination was detected using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) and removed by 1% agarose gel electrophoresis. .. Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Following purification, the mRNA was fragmented into small pieces using divalent cations at elevated temperatures.

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 8.0. .. Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: The total RNA with lowest quality was not used for further study from the pre-receptive endometrium samples and receptive endometrium samples, respectively. .. Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Isolation:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: Paragraph title: 2.2. RNA Isolation, Library Construction and Sequencing ... Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats
    Article Snippet: Paragraph title: RNA Isolation and Transcriptomics ... Total RNA was subjected to poly(A) mRNA enrichment with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, United States).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Two mRNA libraries were constructed for each group (H and L) from total RNAs pooled from two individuals. .. Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the purified mRNA was randomly fragmented using divalent cations under elevated temperature.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: Paragraph title: RNA isolation, preparation of library for DGE ... By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: The quantity and purity of total RNA were analyzed by employing a Bioanalyzer 2100 and RNA 6000 Nano LabChipKit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. The purified mRNA was fragmented into small pieces by divalent cations under elevated temperature.

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: First, only RNA samples that had RNA Integrity Number (RIN) scores > 8 were used for sequencing. .. Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Digital Gene Expression Analysis of Microsporum canis Exposed to Berberine Chloride
    Article Snippet: Paragraph title: RNA isolation and library preparation for DGE ... Approximately 10 μg of total RNA from each sample was subjected to isolate Poly (A) mRNA by using poly-T oligo attached magnetic beads (Invitrogen).

    Purification:

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: Following the manufacturer's protocol, total RNA of each sample was extracted by use of RNAiso Plus reagent (TaKaRa), then purified with absolute alcohol and treated with DNase I. .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: A total amount of 3 μg of RNA per sample was used as the input material for the RNA sample preparations. .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. Fragmentation was carried out using divalent cations at an elevated temperature in NEB First Strand Synthesis reaction buffer (5×) (NEB, USA).

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: The RNA-Seq transcriptome library was prepared using a Truseq RNA Sample Prep Kit (Illumina). .. PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. Then, first-strand complementary DNA (cDNA) was synthesized using reverse transcriptase and random primers, followed by synthesis of second-strand cDNA.

    Polymerase Chain Reaction:

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA).

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The second-strand cDNA was end-repaired using End Repair Mix (Illumina), and a single A base was added at the 3ʹ end for adapter ligation.

    cDNA Library Assay:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the purified mRNA was randomly fragmented using divalent cations under elevated temperature.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. After purification, by use of divalent cations through elevated temperature, the mRNA was split into small fragments.

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Article Title: Transcriptome Analyses Reveal Adult Metabolic Syndrome With Intrauterine Growth Restriction in Pig Models
    Article Snippet: Approximately 5 μg of total RNA from a single liver tissue was isolated by binding of the Poly (A) tails on the mRNA with poly-T oligo conjugated magnetic beads (Thermo Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Digital Gene Expression Analysis of Microsporum canis Exposed to Berberine Chloride
    Article Snippet: Approximately 10 μg of total RNA from each sample was subjected to isolate Poly (A) mRNA by using poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA). .. Following purification, the mRNAs were fragmented into small pieces using divalent cations at an elevated temperature.

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Following purification, the mRNA was fragmented into small pieces using divalent cations at elevated temperatures.

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling
    Article Snippet: Approximately 10 ug of total RNA representing a muscle tissue was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Chromatin Immunoprecipitation:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) and RNA 6000 Nano Lab Chip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: The total RNA quantity and purity were analyzed with Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agi-lent, CA, USA) with RIN number > 7.0. .. Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Liquid Chromatography:

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Then, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA), and the average insert size for the single end libraries was 300 bp (±50 bp).

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. The mRNA is fragmented into small pieces using a divalent cation under elevated temperature after purification, then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Digital Gene Expression Analysis of Microsporum canis Exposed to Berberine Chloride
    Article Snippet: Approximately 10 μg of total RNA from each sample was subjected to isolate Poly (A) mRNA by using poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the single-end libraries was 200bp-350bp.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the six transcriptome library constructions, the RNA preparation, library construction, and high-throughput sequencing were performed by LC-BIO (Hangzhou, China). .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China).

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (± 50 bp).

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Each PE and RE library was constructed by pooling 9 homogenized total RNAs from the endometrium samples.

    Software:

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    RNA Extraction:

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Paragraph title: Total RNA extraction and building of DNA library ... Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: Paragraph title: RNA extraction, transcriptome library preparation, and RNA-Seq ... PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer.

    Selection:

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA).

    Sample Prep:

    Article Title: RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton
    Article Snippet: Approximately 10 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the purified mRNA was randomly fragmented using divalent cations under elevated temperature.

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA. .. After purification, by use of divalent cations through elevated temperature, the mRNA was split into small fragments.

    Article Title: Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
    Article Snippet: Roughly 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

    Article Title: Digital Gene Expression Analysis of Microsporum canis Exposed to Berberine Chloride
    Article Snippet: Approximately 10 μg of total RNA from each sample was subjected to isolate Poly (A) mRNA by using poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the sRNA-seq experiment, approximately 1 μg of total RNA was used to construct an sRNA library, according to the protocol of the TruSeq™ Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: The RNA-Seq transcriptome library was prepared using a Truseq RNA Sample Prep Kit (Illumina). .. PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer.

    Article Title: Transcriptome Analysis of Cadmium-Treated Roots in Maize (Zea mays L.)
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Beijing, China). .. Following purification, the mRNA was fragmented into small pieces using divalent cations at elevated temperatures.

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Spectrophotometry:

    Article Title: Digital gene expression analysis in mice lung with coinfection of influenza and streptococcus pneumoniae
    Article Snippet: The quality and quantity of both DNA and RNA were checked by gel electrophoresis and the DW-K5500 spectrophotometer (DRAWELL). .. By use of poly-T oligo attached magnetic beads (Invitrogen), approximately 10 μg RNA of each sample was employed to separate Poly (A) mRNA.

    Produced:

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. The purified mRNA was fragmented into small pieces by divalent cations under elevated temperature.

    Concentration Assay:

    Article Title: Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness
    Article Snippet: The quality and concentration of total RNA was detected using a RNA 6000 Nano LabChip Kit and a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Poly (A) mRNA was isolated from total RNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    High Throughput Screening Assay:

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: Paragraph title: 4.4. Construction of the mRNA Libraries and High-Throughput Sequencing ... 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the six transcriptome library constructions, the RNA preparation, library construction, and high-throughput sequencing were performed by LC-BIO (Hangzhou, China). .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Fluorescence In Situ Hybridization:

    Article Title: Immunotoxicity of β-Diketone Antibiotic Mixtures to Zebrafish (Danio rerio) by Transcriptome Analysis
    Article Snippet: Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen). .. Poly (A) mRNA was isolated by poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: Paragraph title: Fish and CyHV-2 challenge ... For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

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