poly t oligo  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligo
    Poly T Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo/product/Thermo Fisher
    Average 99 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    poly t oligo - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Amplification:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The cDNA target fragments were selected on 2% Low Range Ultra Agarose (Bio-Red), followed by 15 cycles of PCR amplification.

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    High Throughput Screening Assay:

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: Paragraph title: 4.4. Construction of the mRNA Libraries and High-Throughput Sequencing ... 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Synthesized:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. Then, first-strand complementary DNA (cDNA) was synthesized using reverse transcriptase and random primers, followed by synthesis of second-strand cDNA.

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: .. Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers. .. A library was constructed and sequenced using an Illumina Hiseq 2000/2500 (LC Sciences, USA).

    Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues
    Article Snippet: .. RNA was extracted from the tissues using Trizol (Invitrogen, Carisbad, CA) and first strand cDNA was synthesized using a poly-T oligo and the superscript kit (Invitrogen, Carisbad, CA). .. To select which SNPs to genotype in our samples (see Fig. ), we used a heuristic that prioritized SNPs based on two criteria: (i) The ability of the genotype at a SNP to significantly explain expression in at least two of the three HapMap populations using simple additive linear models and (ii) a minor allele frequency ≥0.2 in the CEU HapMap population.

    Construct:

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Each PE and RE library was constructed by pooling 9 homogenized total RNAs from the endometrium samples.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the sRNA-seq experiment, approximately 1 μg of total RNA was used to construct an sRNA library, according to the protocol of the TruSeq™ Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers. .. A library was constructed and sequenced using an Illumina Hiseq 2000/2500 (LC Sciences, USA).

    Random Hexamer Labeling:

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: .. Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers. .. A library was constructed and sequenced using an Illumina Hiseq 2000/2500 (LC Sciences, USA).

    Expressing:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Paragraph title: 4.4. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System ... Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols.

    Article Title: Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice
    Article Snippet: Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. All raw reads are available as GSE128402 in the NCBI Gene Expression Omnibus.

    Article Title: Expression quantitative trait loci detected in cell lines are often present in primary tissues
    Article Snippet: Given we are primarily interested in the effect of cis regulatory variation, and not the genetic basis of expression covariation among tissues, having largely non-overlapping sets of individuals is preferable to having all tissues from each individual because non-overlapping sets will reduce the impact of individual-specific effects, such as environmental effects, which otherwise could confound the analysis. .. RNA was extracted from the tissues using Trizol (Invitrogen, Carisbad, CA) and first strand cDNA was synthesized using a poly-T oligo and the superscript kit (Invitrogen, Carisbad, CA).

    Ligation:

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The second-strand cDNA was end-repaired using End Repair Mix (Illumina), and a single A base was added at the 3ʹ end for adapter ligation.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Generated:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The libraries were generated by PCR after selection and purification of the cDNA fragments.

    DNA Sequencing:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The cloned IPD083Aa PCR product was confirmed by DNA sequencing.

    Sequencing:

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Paragraph title: Construction of mRNA library and sequencing ... Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: Paragraph title: 4.4. Construction of the mRNA Libraries and High-Throughput Sequencing ... 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Subsequently, we performed the paired-end sequencing on an Illumina Hiseq2500 (LC Sciences, Hongzhou, China), following the recommended protocol from the vendor.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Paragraph title: Library Construction and Sequencing ... Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: Single-end sequencing (50 bp) was performed on the Illumina Hiseq. .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: Paragraph title: mRNA library construction and sequencing ... The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA).

    Article Title: Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice
    Article Snippet: Paragraph title: RNA isolation, library construction and sequencing ... Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptomic Analysis Identifies Candidate Genes and Gene Sets Controlling the Response of Porcine Peripheral Blood Mononuclear Cells to Poly I:C Stimulation
    Article Snippet: Paragraph title: mRNA library construction and sequencing ... For each sample, approximately 5 μg of total RNA was used to isolate poly(A) mRNA using poly-T oligo attached to magnetic beads (Invitrogen).

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Paragraph title: 2.2. mRNA Library Construction and Sequencing ... Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    RNA Sequencing Assay:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: Paragraph title: RNA extraction, transcriptome library preparation, and RNA-Seq ... PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer.

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA). .. Following purification, the mRNAs were fragmented into small pieces using divalent cations at an elevated temperature.

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: Paragraph title: RNA-seq analysis ... Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers.

    Magnetic Beads:

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: .. Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: .. PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. Then, first-strand complementary DNA (cDNA) was synthesized using reverse transcriptase and random primers, followed by synthesis of second-strand cDNA.

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: .. 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. The mRNA is fragmented into small pieces using a divalent cation under elevated temperature after purification, then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: .. Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: .. Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: .. Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. Fragmentation was carried out using divalent cations at an elevated temperature in NEB First Strand Synthesis reaction buffer (5×) (NEB, USA).

    Article Title: Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice
    Article Snippet: .. Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. RNA fragments were reverse-transcribed to create the final cDNA libraries following the NEBNext Ultra RNA Library Prep Kit (Illumina, San Diego, USA), paired-end sequencing was performed.

    Article Title: Transcriptomic Analysis Identifies Candidate Genes and Gene Sets Controlling the Response of Porcine Peripheral Blood Mononuclear Cells to Poly I:C Stimulation
    Article Snippet: .. For each sample, approximately 5 μg of total RNA was used to isolate poly(A) mRNA using poly-T oligo attached to magnetic beads (Invitrogen). .. Following purification, mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: .. Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers. .. A library was constructed and sequenced using an Illumina Hiseq 2000/2500 (LC Sciences, USA).

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: .. Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Isolation:

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice
    Article Snippet: Paragraph title: RNA isolation, library construction and sequencing ... Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: .. Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers. .. A library was constructed and sequenced using an Illumina Hiseq 2000/2500 (LC Sciences, USA).

    RNA Extraction:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: Paragraph title: RNA extraction, transcriptome library preparation, and RNA-Seq ... PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer.

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: Paragraph title: 3.2. Total RNA Extraction and cDNA Library Construction ... Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Purification:

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: .. PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. Then, first-strand complementary DNA (cDNA) was synthesized using reverse transcriptase and random primers, followed by synthesis of second-strand cDNA.

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. The mRNA is fragmented into small pieces using a divalent cation under elevated temperature after purification, then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: .. The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. Fragmentation was carried out using divalent cations at an elevated temperature in NEB First Strand Synthesis reaction buffer (5×) (NEB, USA).

    Article Title: Transcriptomic Analysis Identifies Candidate Genes and Gene Sets Controlling the Response of Porcine Peripheral Blood Mononuclear Cells to Poly I:C Stimulation
    Article Snippet: For each sample, approximately 5 μg of total RNA was used to isolate poly(A) mRNA using poly-T oligo attached to magnetic beads (Invitrogen). .. Following purification, mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
    Article Snippet: RNA-seq analysis Total RNA was extracted using a TRK1001 Total RNA Purification Kit (LC Science, Houston, TX, USA) following the manufacturer’s procedure, then quantified using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0. .. Poly(A) mRNA was isolated from approximately 5 μg total RNA by using magnetic beads with poly-T oligo attached (Invitrogen). cDNA was synthesized using random hexamer primers.

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Polymerase Chain Reaction:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer. .. The cDNA target fragments were selected on 2% Low Range Ultra Agarose (Bio-Red), followed by 15 cycles of PCR amplification.

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The libraries were generated by PCR after selection and purification of the cDNA fragments.

    cDNA Library Assay:

    Article Title: Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose
    Article Snippet: Approximately 5-μg total RNA of each sample was subjected to isolating Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were constructed into the cDNA library according to the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. The mRNA is fragmented into small pieces using a divalent cation under elevated temperature after purification, then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, transcriptomic sequencing ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: Paragraph title: 3.2. Total RNA Extraction and cDNA Library Construction ... Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA). .. The cleaved RNA fragments were then reverse-transcribed to produce the final cDNA library, according to the protocol of the mRNA-seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Transcriptomic Analysis Identifies Candidate Genes and Gene Sets Controlling the Response of Porcine Peripheral Blood Mononuclear Cells to Poly I:C Stimulation
    Article Snippet: For each sample, approximately 5 μg of total RNA was used to isolate poly(A) mRNA using poly-T oligo attached to magnetic beads (Invitrogen). .. Then, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol from the mRNA-seq sample preparation kit (Illumina, San Diego, CA).

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (± 50 bp).

    Mouse Assay:

    Article Title: Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice
    Article Snippet: RNA isolation, library construction and sequencing Entire intervertebral discs from the thoracic and lumbar spine (T8-L5) of the P20 Col2Cre; Adgrg6 f/f and control mice were isolated in cold PBS, snap frozen and pulverized in liquid nitrogen. .. Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Chromatin Immunoprecipitation:

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: The total RNA quantity and purity were analyzed with Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agi-lent, CA, USA) with RIN number > 7.0. .. Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: Total RNA quantity and purity were analyzed using Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agilent, Palo Alto, CA, USA) with RIN number > 7.0 [ , ]. .. Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Plasmid Preparation:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The PCR product was then sub-cloned into a plant transient vector containing the DMMV promoter and a transcriptional terminator with a kanamycin selectable marker (NPTIII) [ ].

    Functional Assay:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: .. Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The gene encoding IPD083Aa was PCR amplified from its respective cDNA sample using primers designed for its coding sequence ( ) with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Selection:

    Article Title: Effects of dietary protein restriction followed by realimentation on growth performance and liver transcriptome alterations of lamb
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo–linked magnetic beads (Invitrogen, USA). .. The libraries were generated by PCR after selection and purification of the cDNA fragments.

    Sample Prep:

    Article Title: Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: The RNA-Seq transcriptome library was prepared using a Truseq RNA Sample Prep Kit (Illumina). .. PolyA mRNA was purified using poly-T oligo attached magnetic beads (Invitrogen), and was randomly segmented into 200-bp fragments by fragmentation buffer.

    Article Title: Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence
    Article Snippet: 0.5 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. The mRNA is fragmented into small pieces using a divalent cation under elevated temperature after purification, then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin
    Article Snippet: Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge
    Article Snippet: Approximately 10 μg of total coelomocyte RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol of the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA), and the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Characterization of the Transcriptional Complexity of the Receptive and Pre-receptive Endometria of Dairy Goats
    Article Snippet: Approximately 5 ug of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: For the sRNA-seq experiment, approximately 1 μg of total RNA was used to construct an sRNA library, according to the protocol of the TruSeq™ Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). .. For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

    Article Title: Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA); the average insert size for the paired-end libraries was 300 bp (± 50 bp).

    Spectrophotometry:

    Article Title: Transcriptomic Analysis Identifies Candidate Genes and Gene Sets Controlling the Response of Porcine Peripheral Blood Mononuclear Cells to Poly I:C Stimulation
    Article Snippet: The integrity and yield of RNA were measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific), and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). .. For each sample, approximately 5 μg of total RNA was used to isolate poly(A) mRNA using poly-T oligo attached to magnetic beads (Invitrogen).

    Marker:

    Article Title: Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
    Article Snippet: Functional Verification of IPD083Aa and its Homologs in the Common Bean Transient Expression System IPD083Aa was cloned using RT-PCR with A. pedatum total RNA preparation. cDNA was generated from 50 ng total RNA by reverse transcription with poly-dT oligo using the SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. The PCR product was then sub-cloned into a plant transient vector containing the DMMV promoter and a transcriptional terminator with a kanamycin selectable marker (NPTIII) [ ].

    Fluorescence In Situ Hybridization:

    Article Title: Integrated analysis of mRNA and viral miRNAs in the kidney of Carassius auratus gibelio response to cyprinid herpesvirus 2
    Article Snippet: Paragraph title: Fish and CyHV-2 challenge ... For the RNA-seq experiment, approximately 10 μg of total RNA was subjected to enrichment for poly (A)-tailed mRNAs using poly-T oligo attached magnetic beads (Invitrogen, MA, USA).

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    Thermo Fisher dynabeads mrna direct purification kit
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