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Illumina Inc poly t oligo
Poly T Oligo, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly t oligo/product/Illumina Inc
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
poly t oligo - by Bioz Stars, 2020-04
92/100 stars

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Related Articles

RNA Extraction:

Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
Article Snippet: .. RNA extraction and processing RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina). .. The cleaved RNA fragments were primed with random hexamers into first strand cDNA followed by reverse transcription using reverse transcriptase (SuperScriptII reverse transcriptase, Invitrogen) and random primers.

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: Paragraph title: RNA extraction and sequencing ... We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol.

Amplification:

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt). .. These cDNA fragments were end-repaired and 3′-adenylated and ligated with adaptors, and then these fragments with adaptors were enriched by means of PCR amplification with Phusion DNA polymerase.

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

RNA Sequencing Assay:

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: Paragraph title: RNA-based next generation sequencing (RNA-Seq) ... Purification of poly - A containing mRNA was performed using poly - T oligo attached magnetic beads (Illumina).

Article Title: Unique properties of thymic antigen-presenting cells promote epigenetic imprinting of alloantigen-specific regulatory T cells
Article Snippet: Paragraph title: RNA-Seq ... Purification of poly- A containing mRNA was done using poly- T oligo attached magnetic beads (Illumina) .

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: Paragraph title: RNA-seq Quantification and Bioinformatics Pipeline. ... Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt).

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: Paragraph title: RNA sequencing and analysis ... Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing.

Magnetic Beads:

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: .. Purification of poly - A containing mRNA was performed using poly - T oligo attached magnetic beads (Illumina). .. Subsequently, mRNA was used for library preparation using the Script Seq v2 Library preparation kit (Illumina).

Article Title: Unique properties of thymic antigen-presenting cells promote epigenetic imprinting of alloantigen-specific regulatory T cells
Article Snippet: .. Purification of poly- A containing mRNA was done using poly- T oligo attached magnetic beads (Illumina) . .. Following the purification, the mRNA was used for library preparation using Script Seq v2 Library preparation kit (Illumina).

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: .. Purification of poly- A containing mRNA was performed using poly- T oligo attached magnetic beads (Illumina). .. Subsequently, mRNA was used for library preparation using the Script Seq v2 Library preparation kit (Illumina).

Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
Article Snippet: .. RNA extraction and processing RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina). .. The cleaved RNA fragments were primed with random hexamers into first strand cDNA followed by reverse transcription using reverse transcriptase (SuperScriptII reverse transcriptase, Invitrogen) and random primers.

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: .. Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt). .. First-strand cDNAs were then synthesized by using SuperScript II reverse transcriptase, and second-strand cDNAs were synthesized by DNA polymerase I.

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: .. We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol. ..

Isolation:

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: Quality and integrity of RNA isolated from brain tissue was controlled on Agilent Technologies 2100 Bioanalyzer (Agilent Technologies). .. Purification of poly - A containing mRNA was performed using poly - T oligo attached magnetic beads (Illumina).

Article Title: Unique properties of thymic antigen-presenting cells promote epigenetic imprinting of alloantigen-specific regulatory T cells
Article Snippet: RNA-Seq Total RNA was isolated from purified APCs with RNAeasy Plus mini kit (Qiagen). .. Purification of poly- A containing mRNA was done using poly- T oligo attached magnetic beads (Illumina) .

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: RNA-based next generation sequencing (RNA-Seq) Quality and integrity of RNA isolated from brain tissue was controlled on Agilent Technologies 2100 Bioanalyzer (Agilent Technologies). .. Purification of poly- A containing mRNA was performed using poly- T oligo attached magnetic beads (Illumina).

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: Total RNA was isolated from ovaries in antagomir-309 or antagomir-control injected mosquitoes. .. Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt).

Next-Generation Sequencing:

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: Paragraph title: RNA-based next generation sequencing (RNA-Seq) ... Purification of poly - A containing mRNA was performed using poly - T oligo attached magnetic beads (Illumina).

Synthesized:

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt). .. First-strand cDNAs were then synthesized by using SuperScript II reverse transcriptase, and second-strand cDNAs were synthesized by DNA polymerase I.

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: .. We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol. ..

Ligation:

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

Purification:

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: .. Purification of poly - A containing mRNA was performed using poly - T oligo attached magnetic beads (Illumina). .. Subsequently, mRNA was used for library preparation using the Script Seq v2 Library preparation kit (Illumina).

Article Title: Unique properties of thymic antigen-presenting cells promote epigenetic imprinting of alloantigen-specific regulatory T cells
Article Snippet: .. Purification of poly- A containing mRNA was done using poly- T oligo attached magnetic beads (Illumina) . .. Following the purification, the mRNA was used for library preparation using Script Seq v2 Library preparation kit (Illumina).

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: .. Purification of poly- A containing mRNA was performed using poly- T oligo attached magnetic beads (Illumina). .. Subsequently, mRNA was used for library preparation using the Script Seq v2 Library preparation kit (Illumina).

Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
Article Snippet: .. RNA extraction and processing RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina). .. The cleaved RNA fragments were primed with random hexamers into first strand cDNA followed by reverse transcription using reverse transcriptase (SuperScriptII reverse transcriptase, Invitrogen) and random primers.

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: .. We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol. ..

Sequencing:

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: Purification of poly - A containing mRNA was performed using poly - T oligo attached magnetic beads (Illumina). .. Sequencing was carried out on Illumina HiSeq.

Article Title: Unique properties of thymic antigen-presenting cells promote epigenetic imprinting of alloantigen-specific regulatory T cells
Article Snippet: Purification of poly- A containing mRNA was done using poly- T oligo attached magnetic beads (Illumina) . .. The sequencing was carried out on Illumina HiSeq2500 using 50 bp single read.

Article Title: Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice
Article Snippet: Purification of poly- A containing mRNA was performed using poly- T oligo attached magnetic beads (Illumina). .. Sequencing was carried out on Illumina HiSeq.

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: The cDNA library for Illumina sequencing was prepared by following the instruction of the TruseqTM RNA sample prep Kit (Illumina). .. Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt).

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: Paragraph title: RNA extraction and sequencing ... We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol.

Polymerase Chain Reaction:

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt). .. These cDNA fragments were end-repaired and 3′-adenylated and ligated with adaptors, and then these fragments with adaptors were enriched by means of PCR amplification with Phusion DNA polymerase.

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

Generated:

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. A quantitative expression profile is generated using Cufflinks 2.2.0 [ ].

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol. .. 100-bp paired end sequences were generated using an Illumina Hi-Seq sequencer.

Expressing:

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. A quantitative expression profile is generated using Cufflinks 2.2.0 [ ].

cDNA Library Assay:

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: The cDNA library for Illumina sequencing was prepared by following the instruction of the TruseqTM RNA sample prep Kit (Illumina). .. Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt).

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: .. Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. Sequencing was then carried out on paired-end 75 bb using Illumina NextSeq500.

Sample Prep:

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: The cDNA library for Illumina sequencing was prepared by following the instruction of the TruseqTM RNA sample prep Kit (Illumina). .. Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt).

Injection:

Article Title: microRNA-309 targets the Homeobox gene SIX4 and controls ovarian development in the mosquito Aedes aegypti
Article Snippet: Total RNA was isolated from ovaries in antagomir-309 or antagomir-control injected mosquitoes. .. Briefly, mRNA was enriched from total RNA by using poly-T oligo–attached magnetic beads (Illumina) and subsequently disrupted into fragments (200–700 nt).

Chromatin Immunoprecipitation:

Article Title: Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain
Article Snippet: RNA quality was determined using the Agilent 2100 Bioanalyzer RNA Nano Chip (Mean RIN = 9.22, RIN Range = 7.8–9.6). .. We purified poly(A)+RNA from 4 μg total RNA using poly-T oligo attached magnetic beads and synthesized cDNA libraries using the TruSeq RNA sample preparations kit with random priming (Illumina cat. no. RS-122-2001) as per the manufacturer's protocol.

Software:

Article Title: The histomolecular criteria established for adult anaplastic pilocytic astrocytoma are not applicable to the pediatric population
Article Snippet: Briefly, the key stages of this protocol were, successively, the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 1 µg total RNA, a fragmentation using divalent cations under elevated temperature to obtain approximately 300 bp fragments, double-stranded cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. .. The software used for RNA alignment was TopHat2.1.0, including Bowtie2.

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    Illumina Inc poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Illumina Inc
    Average 96 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    poly t oligo attached magnetic beads - by Bioz Stars, 2020-04
    96/100 stars
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