poly t oligo beads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligo beads
    Poly T Oligo Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo beads/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poly t oligo beads - by Bioz Stars, 2020-04
    94/100 stars

    Related Products / Commonly Used Together

    ribosomal rna
    rna
    mrna library preparation

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    Related Articles

    RNA Extraction:

    Article Title: Integrated microRNA and mRNA responses to acute human left ventricular ischemia
    Article Snippet: Tissue samples were immediately placed in RNAlater (Ambion, Life Technologies) at +4°C for 48 h and then frozen at −80°C until RNA extraction. .. For mRNA library preparation, ribosomal RNA was removed by performing one or two washings of RNA annealed to poly-T oligo beads (Invitrogen; Life Technologies, Grand Island, NY).

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Tissue samples were immediately placed in RNAlater® (Ambion, Life Technologies, USA) at +4C for 48 hours and then frozen at −80C until RNA extraction. .. Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY).

    RNA Sequencing Assay:

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Paragraph title: RNA sequencing ... Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY).

    Ligation:

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with EB buffer for end reparation and poly(A) addition followed by ligation with sequencing adaptors for cluster generation and non-strand tagged sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Isolation:

    Article Title: Integrated microRNA and mRNA responses to acute human left ventricular ischemia
    Article Snippet: Total RNA was isolated with Trizol, and RNA quality was assessed using the Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA). .. For mRNA library preparation, ribosomal RNA was removed by performing one or two washings of RNA annealed to poly-T oligo beads (Invitrogen; Life Technologies, Grand Island, NY).

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Total RNA was isolated with Trizol and RNA quality was assessed using the Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA). .. Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY).

    Purification:

    Article Title: Integrated microRNA and mRNA responses to acute human left ventricular ischemia
    Article Snippet: For mRNA library preparation, ribosomal RNA was removed by performing one or two washings of RNA annealed to poly-T oligo beads (Invitrogen; Life Technologies, Grand Island, NY). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with elution buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with EB buffer for end reparation and poly(A) addition followed by ligation with sequencing adaptors for cluster generation and non-strand tagged sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Sequencing:

    Article Title: Integrated microRNA and mRNA responses to acute human left ventricular ischemia
    Article Snippet: Paragraph title: miRNA and mRNA sequencing. ... For mRNA library preparation, ribosomal RNA was removed by performing one or two washings of RNA annealed to poly-T oligo beads (Invitrogen; Life Technologies, Grand Island, NY).

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with EB buffer for end reparation and poly(A) addition followed by ligation with sequencing adaptors for cluster generation and non-strand tagged sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Integrated microRNA and mRNA responses to acute human left ventricular ischemia
    Article Snippet: For mRNA library preparation, ribosomal RNA was removed by performing one or two washings of RNA annealed to poly-T oligo beads (Invitrogen; Life Technologies, Grand Island, NY). .. For miRNA library preparation, RNA was separated by polyacrylamide gel electrophoresis, and the 18–30 nucleotide stripe was selected.

    Polymerase Chain Reaction:

    Article Title: Integrated microRNA and mRNA responses to acute human left ventricular ischemia
    Article Snippet: For mRNA library preparation, ribosomal RNA was removed by performing one or two washings of RNA annealed to poly-T oligo beads (Invitrogen; Life Technologies, Grand Island, NY). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with elution buffer for end reparation and poly(A) addition then ligated with sequencing adaptors for cluster generation and sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Article Title: The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle
    Article Snippet: Ribosomal RNA was removed by performing 1–2 washings of RNA annealed to poly-T oligo beads (Invitrogen, Life technologies, Grand Island, NY). .. Double-stranded DNA (dsDNA) synthesis was performed using Pol I and RNA-ase H. Short fragments were purified with QiaQuick PCR extraction kit (Qiagen, Hilden, Germany) and resolved with EB buffer for end reparation and poly(A) addition followed by ligation with sequencing adaptors for cluster generation and non-strand tagged sequencing on the Illumina HiSeq 2000 (Illumina, San Diego, CA).

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    Thermo Fisher oligo
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    Thermo Fisher poly t oligo attached magnetic beads
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    Thermo Fisher poly t oligos
    Poly T Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligos/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
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