poly t oligo attached magnetic beads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Thermo Fisher
    Average 94 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    poly t oligo attached magnetic beads - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Suitable fragments were selected as templates for PCR amplification and abundance normalization.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. After PCR amplification, the PCR yields were quantified by Qubit (Invitrogen) and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final cDNA library.

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA. .. The mRNA was fragmented, and the RNA fragments were reverse transcribed and amplified to double-stranded cDNA.

    Synthesized:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies). .. The first cDNA strand was synthesized using random oligonucleotides and M-MuLV Reverse Transcriptase (RNase H-).

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Construct:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: A single cDNA library was constructed. .. The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. 24 DGE libraries were constructed with Illumina's DGE Tag Profiling Kit according to the manufacturer's protocol (Version 2.1B).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. The PCR was performed to amplify cDNAs to construct the library.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Real-time Polymerase Chain Reaction:

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. During the QC steps, an Agilent 2100 Bioanaylzer (Agilent, Santa Clara, CA, USA) and an ABI StepOnePlus Real-Time PCR System (ABI, Waltham, MA, USA) were used in quantification and qualification of the sample library.

    Incubation:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Cell Culture:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: The hTERT-BTY cells were cultured for 24 h. The O/BY/CHA/2010 was inoculated with 0.001 PFU/cell. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Expressing:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Transcriptome RNA-Seq The differential expression of mRNA in infected and uninfected hTERT-BTY cells was detected to identify the pathways of innate immunity using RNA-seq. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Ligation:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. An A-base was then added to the blunt ends of each strand, preparing them for ligation with the indexed adapters.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Infection:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Transcriptome RNA-Seq The differential expression of mRNA in infected and uninfected hTERT-BTY cells was detected to identify the pathways of innate immunity using RNA-seq. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Generated:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies). .. 101 bp paired-end reads were generated and all raw sequence read data were stored in FastQ format.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. DNA nanoballs (DNBs) were generated with the ssDNA circle by rolling circle replication (RCR) to enlarge the fluorescent signals at the sequencing process.

    Polymerase Chain Reaction:

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Suitable fragments were selected as templates for PCR amplification and abundance normalization.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. The PCR was performed to amplify cDNAs to construct the library.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. After PCR amplification, the PCR yields were quantified by Qubit (Invitrogen) and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final cDNA library.

    RNA Sequencing Assay:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Paragraph title: Transcriptome RNA-Seq ... Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Paragraph title: 4.3. RNA Isolation, Library Construction and RNA Sequencing ... Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Paragraph title: cDNA library construction and RNA sequencing ... Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Magnetic Beads:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Short fragments were purified and resolved with EB buffer (Qiagen, Valencia, CA, USA) for end reparation and single nucleotide A (adenine) addition.

    Article Title: Deep Sequencing of the Transcriptome Reveals Inflammatory Features of Porcine Visceral Adipose Tissue
    Article Snippet: .. Approximately 30 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: .. Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA). .. After purification, the poly(A)− or the poly(A)+ RNA fractions were fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: .. The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies). .. The first cDNA strand was synthesized using random oligonucleotides and M-MuLV Reverse Transcriptase (RNase H-).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: .. Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. ..

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: .. Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA. .. The mRNA was fragmented, and the RNA fragments were reverse transcribed and amplified to double-stranded cDNA.

    Isolation:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Short fragments were purified and resolved with EB buffer (Qiagen, Valencia, CA, USA) for end reparation and single nucleotide A (adenine) addition.

    Article Title: Deep Sequencing of the Transcriptome Reveals Inflammatory Features of Porcine Visceral Adipose Tissue
    Article Snippet: For minimize the effect of transcriptome unevenness among individuals, equal quantities (10 µg) of total RNA isolated from three female pigs were pooled. .. Approximately 30 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Paragraph title: RNA isolation, cDNA library, and illumina sequencing ... Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: .. Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Total RNA was isolated from Cd-free and Cd-treated peanut roots of both cultivars using Trizol® Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Purification:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: Transcriptome Sequencing Total RNA was extracted from the venom glands of 10 scorpions using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using RNeasy Animal Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature.

    Article Title: Deep Sequencing of the Transcriptome Reveals Inflammatory Features of Porcine Visceral Adipose Tissue
    Article Snippet: Approximately 30 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA). .. After purification, the poly(A)− or the poly(A)+ RNA fractions were fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: .. The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies). .. The first cDNA strand was synthesized using random oligonucleotides and M-MuLV Reverse Transcriptase (RNase H-).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. ..

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: .. Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA. .. The mRNA was fragmented, and the RNA fragments were reverse transcribed and amplified to double-stranded cDNA.

    Sequencing:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: Paragraph title: 5.2. Transcriptome Sequencing ... The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature.

    Article Title: Deep Sequencing of the Transcriptome Reveals Inflammatory Features of Porcine Visceral Adipose Tissue
    Article Snippet: Paragraph title: mRNA library construction and sequencing ... Approximately 30 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Paragraph title: RNA isolation, cDNA library, and illumina sequencing ... Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the paired-end sequencing was performed on an Illumina Hiseq 4000 (LC Sciences, United States) following the manufacturer’s recommended protocol.

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: RNA Isolation, Library Construction and RNA Sequencing Soybean leaves (the first trifoliate true leaves) were collected after Pi-deprivation treatment for 24 h. Four samples (two biological replicates of both Pi-deprived and Pi-sufficient leaves) were used for mRNA library construction and sequencing. .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: Paragraph title: cDNA library construction and Illumina sequencing ... The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: Paragraph title: RNA extraction and deep sequencing ... Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA.

    cDNA Library Assay:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: Deep Sequencing of the Transcriptome Reveals Inflammatory Features of Porcine Visceral Adipose Tissue
    Article Snippet: Approximately 30 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 200 bp (±20 bp).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Paragraph title: RNA isolation, cDNA library, and illumina sequencing ... Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: Paragraph title: cDNA library construction and Illumina sequencing ... The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Paragraph title: cDNA library construction and RNA sequencing ... Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Sample Prep:

    Article Title: Deep Sequencing of the Transcriptome Reveals Inflammatory Features of Porcine Visceral Adipose Tissue
    Article Snippet: Approximately 30 µg of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 200 bp (±20 bp).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: The sequencing library for each RNA sample was prepared using a TruseqTM RNA sample prep Kit (Illumina, San Diego, CA, USA), following the manufacturer’s protocol. .. Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA.

    RNA Extraction:

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Paragraph title: RNA extraction and library preparation ... Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: Paragraph title: RNA extraction and deep sequencing ... Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA.

    Agarose Gel Electrophoresis:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: After extraction, the concentration and purity of RNA were checked by an NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) at 260 nm; and the integrity of the total RNA was measured by 1.2% agarose gel electrophoresis and an Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Spectrophotometry:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: After extraction, the concentration and purity of RNA were checked by an NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) at 260 nm; and the integrity of the total RNA was measured by 1.2% agarose gel electrophoresis and an Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Then the RNA integrity and purity were assessed using Agilent 2100 Bioanalyzer (Agilent, USA) and NanoDrop™ spectrophotometer (Thermo Fisher Scientific, USA), respectively. .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Concentration Assay:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: RNA integrity was assessed using the RNA 6000 Nano Assay Kit with a Bioanalyzer 2100 (Agilent Technologies) after checking the RNA purity and concentration. .. The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: The high-quality RNA samples for library construction were selected according to 260/280 nm ratio (1.9–2.1), RNA concentration (≥ 500 ng/μL) and RNA integrity number (RIN ≥ 8.0), respectively (Additional file : Table S2). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Dissection of the style’s response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species
    Article Snippet: The RNA concentration of each sample was measured using a NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA). .. Briefly, mRNA was purified using poly-T oligo-attached magnetic beads (Invitrogen,Carlsbad, CA, USA) from 5 μg total RNA.

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    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 31 article reviews
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    poly t oligo attached magnetic beads - by Bioz Stars, 2020-04
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