poly t oligo attached magnetic beads  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    poly t oligo attached magnetic beads - by Bioz Stars, 2019-12
    99/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. The short cDNA fragments generated were purified, end-repaired, poly-adenylated and ligated to indexed sequencing adaptors.

    Synthesized:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. Following purification, the mRNA was fragmented using fragmentation buffer.

    Article Title: Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum
    Article Snippet: Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol. .. Purified mRNA was then divided into short fragments by mixing with fragmentation buffer.

    Construct:

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice
    Article Snippet: 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher). .. 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Electrophoresis:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The total RNA was isolated from each sample using TRIzol® , and the degradation and contamination of RNA was assessed using agarose gel (Abcam, Cambridge, MA, USA) electrophoresis. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Random Hexamer Labeling:

    Article Title: Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum
    Article Snippet: Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol. .. Purified mRNA was then divided into short fragments by mixing with fragmentation buffer.

    Cell Culture:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: The hTERT-BTY cells were cultured for 24 h. The O/BY/CHA/2010 was inoculated with 0.001 PFU/cell. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Expressing:

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq.

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: The differential expression of mRNA in infected and uninfected hTERT-BTY cells was detected to identify the pathways of innate immunity using RNA-seq. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Modification:

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. Random hexamers were then used for first cDNA strand synthesis, following which the second strand of cDNA was synthesized after the supplementation of buffer, dNTPs, and DNA Polymerase I.

    Gel Purification:

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. To selectively enrich library fragments ranging from 150 to 200 bp in length, the ligated cDNA molecules were purified with AMPure XP system (Beckman Coulter, Beverly, USA), and used as templates for PCR amplification based on the results of agarose gel electrophoresis.

    Ligation:

    Article Title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice
    Article Snippet: 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was cleaved into small pieces using divalent cations and under elevated temperature and used to construct a final cDNA library according to the protocol for the Illumina RNA ligation method (Illumina, San Diego, USA).

    Infection:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: The differential expression of mRNA in infected and uninfected hTERT-BTY cells was detected to identify the pathways of innate immunity using RNA-seq. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Generated:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. After PCR amplification, the PCR yields were quantified by Qubit (Invitrogen) and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final cDNA library.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature.

    Polymerase Chain Reaction:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA). .. Second-strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H; double-stranded cDNA was purified and quantified.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. The short cDNA fragments generated were purified, end-repaired, poly-adenylated and ligated to indexed sequencing adaptors.

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. The cleaved cDNA purified by AMPure XP beads (Illumina, San Diego, USA) was modified by end-repair, and addition of A-tail and adapter.

    Article Title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice
    Article Snippet: 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher). .. Briefly, purified RNA with the RNeasy MinElute Kit (Qiagen) was ligated with a pre-adenylated 3' adapter which enables the subsequent ligation of a 5' adapter.

    RNA Sequencing Assay:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: RNA-Seq library construction was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA) following manufacturer’s instructions. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Paragraph title: 4.3. RNA Isolation, Library Construction and RNA Sequencing ... Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Paragraph title: cDNA library construction and RNA sequencing ... Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Paragraph title: RNA isolation and library construction for RNA-seq analysis ... Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: The high quality RNA were obtained through twice Chloroform/Isoamyl alcohol (24:1) purification, and then mixed with approximately the same quantity. .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. .. Different tissues of black pepper were collected (root, stem, leaf, flower, and fruits).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Paragraph title: Transcriptome RNA-Seq ... Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice
    Article Snippet: Paragraph title: RNA-sequencing analysis ... 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher).

    Article Title: Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum
    Article Snippet: Paragraph title: Construction of the cDNA library and de novo RNA-Seq ... Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol.

    Magnetic Beads:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Four index codes were added to assign sequences for each sample. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA). .. Fragmentation was performed using divalent cations under elevated temperature (55°C) in Illumina proprietary fragmentation buffer (Illumina).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: The RIN (RNA integrity number) of all RNA samples were determined to be more than 7.0. .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: The quantity and purity of the extracted RNA were estimated using the Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Palo Alto, CA, USA) with the RNA integrity number > 8.0. .. Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: The high quality RNA were obtained through twice Chloroform/Isoamyl alcohol (24:1) purification, and then mixed with approximately the same quantity. .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. .. Different tissues of black pepper were collected (root, stem, leaf, flower, and fruits).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. These fragmented mRNAs were then used as templates to synthesize cDNA by reverse transcriptase and random primers.

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: The total RNA quantity and purity were analyzed using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, United States) with RIN number > 7.0. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: RNA quantity and purity were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: Total RNA from the seed coats of ZB107 and ZB306 were extracted using RNAprep pure Tissue Kit (Tiangen, Beijing, China) and the integrity and quality of the RNA was checked by 1% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA). .. Fragmentation of the mRNA was carried out and the cleaved RNA fragments were copied into first-strand cDNA using reverse transcriptase and random primers.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: RNA integrity number (RIN) values were measured for all samples. .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Short fragments were purified and resolved with EB buffer (Qiagen, Valencia, CA, USA) for end reparation and single nucleotide A (adenine) addition.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Purity and integrity of RNA was checked with the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) with RIN number > 6.8. .. Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. The cleaved RNA fragments were reverse-transcribed to generate first-strand cDNA, accompanied with second-strand cDNA synthesis using Invitrogen DNA Polymerase I and RNase H (New England Biolabs).

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Total RNA quantity and purity were analyze using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Palo Alto, CA, USA) with RIN > 8.0. .. Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Qubit® 2.0 fluorometer was used for determination of RNA concentration. .. Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. Following purification, the mRNA was fragmented using fragmentation buffer.

    Article Title: Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum
    Article Snippet: Finally, RNA was dissolved in RNase-free ddH2 O and stored at − 80 °C until further use. .. Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol. .. Purified mRNA was then divided into short fragments by mixing with fragmentation buffer.

    Isolation:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, and sequencing ... In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The total RNA was isolated from each sample using TRIzol® , and the degradation and contamination of RNA was assessed using agarose gel (Abcam, Cambridge, MA, USA) electrophoresis. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: The RIN (RNA integrity number) of all RNA samples were determined to be more than 7.0. .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Total RNA was isolated from Cd-free and Cd-treated peanut roots of both cultivars using Trizol® Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Paragraph title: RNA isolation and library construction for RNA-seq analysis ... Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: Paragraph title: Plant materials and nucleic acid isolation ... Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: RNA quantity and purity were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: RNA integrity number (RIN) values were measured for all samples. .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Short fragments were purified and resolved with EB buffer (Qiagen, Valencia, CA, USA) for end reparation and single nucleotide A (adenine) addition.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Purity and integrity of RNA was checked with the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) with RIN number > 6.8. .. Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. The cleaved RNA fragments were reverse-transcribed to generate first-strand cDNA, accompanied with second-strand cDNA synthesis using Invitrogen DNA Polymerase I and RNase H (New England Biolabs).

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Paragraph title: RNA isolation and library construction for transcriptome analysis ... Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Qubit® 2.0 fluorometer was used for determination of RNA concentration. .. Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. Following purification, the mRNA was fragmented using fragmentation buffer.

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Total RNA quantity and purity were examined by Bioanalyzer (Agilent). .. Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures.

    Purification:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Four index codes were added to assign sequences for each sample. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA). .. Fragmentation was performed using divalent cations under elevated temperature (55°C) in Illumina proprietary fragmentation buffer (Illumina).

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: The high quality RNA were obtained through twice Chloroform/Isoamyl alcohol (24:1) purification, and then mixed with approximately the same quantity. .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. .. Different tissues of black pepper were collected (root, stem, leaf, flower, and fruits).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. These fragmented mRNAs were then used as templates to synthesize cDNA by reverse transcriptase and random primers.

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: Total RNA from the seed coats of ZB107 and ZB306 were extracted using RNAprep pure Tissue Kit (Tiangen, Beijing, China) and the integrity and quality of the RNA was checked by 1% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA). .. Fragmentation of the mRNA was carried out and the cleaved RNA fragments were copied into first-strand cDNA using reverse transcriptase and random primers.

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: Total RNA was extracted from the venom glands of 10 scorpions using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using RNeasy Animal Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature.

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Total RNA extraction, mRNA purification, and cDNA library construction were conducted by LC Sciences (Houston, TX, USA). .. Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. Random hexamers were then used for first cDNA strand synthesis, following which the second strand of cDNA was synthesized after the supplementation of buffer, dNTPs, and DNA Polymerase I.

    Article Title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice
    Article Snippet: 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher). .. Following purification, the mRNA was cleaved into small pieces using divalent cations and under elevated temperature and used to construct a final cDNA library according to the protocol for the Illumina RNA ligation method (Illumina, San Diego, USA).

    Article Title: Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum
    Article Snippet: Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol. .. Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol.

    Sequencing:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, and sequencing ... In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Soybean leaves (the first trifoliate true leaves) were collected after Pi-deprivation treatment for 24 h. Four samples (two biological replicates of both Pi-deprived and Pi-sufficient leaves) were used for mRNA library construction and sequencing. .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Paragraph title: 4.2. mRNA Library Construction and Sequencing ... Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: Paragraph title: 4.4. RNA Extraction, Cdna Preparation and Illumina Sequencing ... The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA).

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: Paragraph title: 5.2. Transcriptome Sequencing ... The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature.

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Paragraph title: Library construction and transcriptome sequencing ... Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature.

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq Sample Preparation Kit (Illumina, San Diego, CA, USA), and the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Paragraph title: cDNA library construction and Illumina sequencing ... Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA).

    Article Title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice
    Article Snippet: 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher). .. 10 ug of total RNA extracted from osfie2-1 and wild-type were used to isolate Poly-(A) mRNA using poly-T oligo-attached magnetic beads (Thermo-Fisher).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen).

    cDNA Library Assay:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, and sequencing ... In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Paragraph title: cDNA library construction and RNA sequencing ... Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Total RNA extraction, mRNA purification, and cDNA library construction were conducted by LC Sciences (Houston, TX, USA). .. Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Paragraph title: cDNA library construction and Illumina sequencing ... Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: Transcriptomic de novo analysis of pitaya (Hylocereus polyrhizus) canker disease caused by Neoscytalidium dimidiatum
    Article Snippet: Paragraph title: Construction of the cDNA library and de novo RNA-Seq ... Poly (A) mRNA from the total RNA was enriched by poly-T oligo-attached magnetic beads (Invitrogen, Thermo Fisher Scientific, CN), according to the manufacturer’s protocol.

    Agarose Gel Electrophoresis:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The total RNA was isolated from each sample using TRIzol® , and the degradation and contamination of RNA was assessed using agarose gel (Abcam, Cambridge, MA, USA) electrophoresis. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: After extraction, the concentration and purity of RNA were checked by an NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) at 260 nm; and the integrity of the total RNA was measured by 1.2% agarose gel electrophoresis and an Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: Total RNA from the seed coats of ZB107 and ZB306 were extracted using RNAprep pure Tissue Kit (Tiangen, Beijing, China) and the integrity and quality of the RNA was checked by 1% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA).

    Article Title: Comparative transcriptome analysis reveals potentially novel roles of Homeobox genes in adipose deposition in fat-tailed sheep
    Article Snippet: Poly(A) mRNA isolated from approximately 3 μg of total RNA with poly-T oligo-attached magnetic beads (Invitrogen) was cleaved into shorter fragments using a metal catalyst under elevated temperature. .. The short cDNA fragments generated were purified, end-repaired, poly-adenylated and ligated to indexed sequencing adaptors.

    Chromatin Immunoprecipitation:

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: RNA quantity and purity were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Liquid Chromatography:

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq Sample Preparation Kit (Illumina, San Diego, CA, USA), and the average insert size for the paired-end libraries was 300 bp (±50 bp).

    Real-time Polymerase Chain Reaction:

    Article Title: A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
    Article Snippet: The RIN of M. martensii was 7.7. mRNA was isolated from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA)and was fragmented into short fragments (200–700 bp) using divalent cations under elevated temperature. .. Suitable fragments were selected as templates for PCR amplification and abundance normalization.

    RNA Extraction:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Paragraph title: RNA extraction and library preparation ... Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: Paragraph title: 4.4. RNA Extraction, Cdna Preparation and Illumina Sequencing ... The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA).

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Total RNA extraction, mRNA purification, and cDNA library construction were conducted by LC Sciences (Houston, TX, USA). .. Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen).

    Selection:

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA). .. Second-strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H; double-stranded cDNA was purified and quantified.

    Article Title: Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages
    Article Snippet: Total RNA obtained from goats of the same group was pooled in equal quantities (1 μg mRNA per goat). mRNA was isolated with poly-T-oligo-attached magnetic beads (Invitrogen, USA). .. The cleaved cDNA purified by AMPure XP beads (Illumina, San Diego, USA) was modified by end-repair, and addition of A-tail and adapter.

    Sample Prep:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress
    Article Snippet: Approximately 10 μg of total RNA of each liver sample was used to isolate Poly(A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways
    Article Snippet: Approximately 10 μg total RNA representing a specific type was subjected to mRNA isolation with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures.

    Next-Generation Sequencing:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: Paragraph title: Next-generation sequencing (NGS) ... Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Spectrophotometry:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: RNA was checked on 1% agarose gels to avoid possible degradation and contamination, and was then examined by a Nano Photometer spectrophotometer (IMPLEN, CA, USA) for RNA purity. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Then the RNA integrity and purity were assessed using Agilent 2100 Bioanalyzer (Agilent, USA) and NanoDrop™ spectrophotometer (Thermo Fisher Scientific, USA), respectively. .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: After extraction, the concentration and purity of RNA were checked by an NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) at 260 nm; and the integrity of the total RNA was measured by 1.2% agarose gel electrophoresis and an Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
    Article Snippet: Total RNA from the seed coats of ZB107 and ZB306 were extracted using RNAprep pure Tissue Kit (Tiangen, Beijing, China) and the integrity and quality of the RNA was checked by 1% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. The mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA).

    Concentration Assay:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA) was used to measure RNA concentration, and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used to evaluate RNA integrity. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: The high-quality RNA samples for library construction were selected according to 260/280 nm ratio (1.9–2.1), RNA concentration (≥ 500 ng/μL) and RNA integrity number (RIN ≥ 8.0), respectively (Additional file : Table S2). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    poly t oligo attached magnetic beads - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    Image Search Results