poly t oligo attached magnetic beads  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Thermo Fisher
    Average 99 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    poly t oligo attached magnetic beads - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. After PCR amplification, the PCR yields were quantified by Qubit (Invitrogen) and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final cDNA library.

    Synthesized:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA). .. First strand cDNA was synthesized using random oligonucleotides and SuperScript II (Abcam).

    Construct:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. The PCR was performed to amplify cDNAs to construct the library.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Electrophoresis:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The total RNA was isolated from each sample using TRIzol® , and the degradation and contamination of RNA was assessed using agarose gel (Abcam, Cambridge, MA, USA) electrophoresis. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Incubation:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Cell Culture:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: The hTERT-BTY cells were cultured for 24 h. The O/BY/CHA/2010 was inoculated with 0.001 PFU/cell. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Expressing:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Transcriptome RNA-Seq The differential expression of mRNA in infected and uninfected hTERT-BTY cells was detected to identify the pathways of innate immunity using RNA-seq. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: RNA Sequencing and Data Analysis To analyze the relationship between DNA methylation and gene expression, watermelon leaves were also collected for RNA extraction at 0 h, 48 h, and 25 d post-CGMMV inoculation. .. Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. .. Total RNA was also isolated from each tissue for further expression analysis.

    Hybridization:

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Ligation:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. An A-base was then added to the blunt ends of each strand, preparing them for ligation with the indexed adapters.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Infection:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Transcriptome RNA-Seq The differential expression of mRNA in infected and uninfected hTERT-BTY cells was detected to identify the pathways of innate immunity using RNA-seq. .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Generated:

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. After the transcriptome data was generated.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. DNA nanoballs (DNBs) were generated with the ssDNA circle by rolling circle replication (RCR) to enlarge the fluorescent signals at the sequencing process.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Polymerase Chain Reaction:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. .. The PCR was performed to amplify cDNAs to construct the library.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. To select cDNA fragments of the preferred 200 bp in length, the library fragments were purified using an AMPure XP system (Beckman Coulter, Beverly, CA, USA). cDNA fragments with ligated adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 12-cycle PCR reaction.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. After PCR amplification, the PCR yields were quantified by Qubit (Invitrogen) and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final cDNA library.

    RNA Sequencing Assay:

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Paragraph title: Transcriptome RNA-Seq ... Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Paragraph title: 4.3. RNA Isolation, Library Construction and RNA Sequencing ... Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: Paragraph title: 2.9. RNA Sequencing and Data Analysis ... Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: RNA-Seq library construction was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA) following manufacturer’s instructions. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Paragraph title: cDNA library construction and RNA sequencing ... Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Paragraph title: RNA isolation and library construction for RNA-seq analysis ... Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. ..

    Magnetic Beads:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: .. Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: .. Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA). .. After purification, the poly(A)− or the poly(A)+ RNA fractions were fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: .. Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. ..

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: .. Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces and then reverse-transcribed to create the final cDNA library.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments. .. Next, the first-strand cDNA was synthesized using reverse transcriptase and random primers with small fragments of mRNA as templates; and the second-strand cDNA was synthesized via DNA polymerase I and RNase H. Thereafter, the double-stranded cDNA ends were further repaired by a single nucleotide ‘A’ base addition, and subsequent ligation of the adapter.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA). .. Fragmentation was performed using divalent cations under elevated temperature (55°C) in Illumina proprietary fragmentation buffer (Illumina).

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: .. Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. ..

    Isolation:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: .. Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Paragraph title: RNA isolation, cDNA library, and illumina sequencing ... Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: .. Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces and then reverse-transcribed to create the final cDNA library.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, and sequencing ... In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: cDNA library construction and RNA sequencing Total RNA was isolated from Cd-free and Cd-treated peanut roots of both cultivars using Trizol® Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The total RNA was isolated from each sample using TRIzol® , and the degradation and contamination of RNA was assessed using agarose gel (Abcam, Cambridge, MA, USA) electrophoresis. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Paragraph title: RNA isolation and library construction for RNA-seq analysis ... Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: Paragraph title: Plant materials and nucleic acid isolation ... Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq.

    Purification:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under an elevated temperature.

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA). .. After purification, the poly(A)− or the poly(A)+ RNA fractions were fragmented into small pieces using divalent cations under elevated temperatures.

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces using divalent cations under elevated temperature after purification.

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases. ..

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H− ), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA). .. Fragmentation was performed using divalent cations under elevated temperature (55°C) in Illumina proprietary fragmentation buffer (Illumina).

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

    Article Title: De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)
    Article Snippet: .. Total 5μg mRNA was purified using poly-T oligo-attached magnetic beads (Life Technologies) for RNA-Seq. ..

    Sequencing:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Paragraph title: 4.2. mRNA Library Construction and Sequencing ... Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Paragraph title: RNA isolation, cDNA library, and illumina sequencing ... Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the paired-end sequencing was performed on an Illumina Hiseq 4000 (LC Sciences, United States) following the manufacturer’s recommended protocol.

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: RNA Isolation, Library Construction and RNA Sequencing Soybean leaves (the first trifoliate true leaves) were collected after Pi-deprivation treatment for 24 h. Four samples (two biological replicates of both Pi-deprived and Pi-sufficient leaves) were used for mRNA library construction and sequencing. .. Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The paired-end sequencing was carried out on an Illumina HiSeq 4000 platform.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, and sequencing ... In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Eight cDNA libraries named FCK _1, FCK _2, FCd _1, FCd _2, SCK _1, SCK _2, SCd _1 and SCd _2 were constructed with the BGISEQ-500 platform combining the DNA nanoball-based nanoarrays and stepwise sequencing using combinational probe-anchor synthesis sequencing method (BGI, Shenzhen, China). .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    cDNA Library Assay:

    Article Title: Revealing the pathogenic changes of PAH based on multiomics characteristics
    Article Snippet: .. cDNA library construction and sequencing Poly(A) mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads (Invitrogen, Waltham, US). .. Following purification, the mRNA was fragmented into small pieces using divalent cations and incubated with Fragmentation Buffer (Illumina) in a preheated tube for 5 min at 94 °C.

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Then, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Paragraph title: RNA isolation, cDNA library, and illumina sequencing ... Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The mRNA was fragmented into small pieces and then reverse-transcribed to create the final cDNA library.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Paragraph title: RNA isolation, cDNA library construction, and sequencing ... In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Paragraph title: cDNA library construction and RNA sequencing ... Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the cleaved RNA fragments were reverse transcribed to create the final complementary DNA (cDNA) library in accordance with the protocol for the mRNA-Seq Sample Preparation Kit (Illumina, San Diego, CA, USA).

    Sample Prep:

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA). .. Then, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: Transcriptome sequencing, molecular markers, and transcription factor discovery of Platanus acerifolia in the presence of Corythucha ciliata
    Article Snippet: Approximately 10 μg of the total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen, CA, USA). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).

    Article Title: Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus
    Article Snippet: Approximately 10 μg of total RNA representing a specific adipose type was used to isolate Poly (A) mRNA with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the mRNA was fragmented into small pieces using divalent cations under elevated temperature, and the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, United States).

    Article Title: Early Transcriptomic Response to Phosphate Deprivation in Soybean Leaves as Revealed by RNA-Sequencing
    Article Snippet: Approximately 10 µg of total RNA was subjected to Poly (A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen). .. The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA).

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Sequencing libraries were prepared by the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s guidelines. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The pooled samples were then used to prepare six separate Illumina sequencing libraries, containing three technical replicates for each condition. cDNA libraries were generated using an Illumina TruSeq™ RNA Sample Preparation kit (Illumina), according to the manufacturer's instructions, and index codes were added to attribute sequences to each sample. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Article Title: Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck
    Article Snippet: Approximately 10 μg of total RNA of each sample was used to isolate poly(A) messenger RNA (mRNA) with poly-T oligo-attached magnetic beads (Invitrogen). .. Then, the cleaved RNA fragments were reverse transcribed to create the final complementary DNA (cDNA) library in accordance with the protocol for the mRNA-Seq Sample Preparation Kit (Illumina, San Diego, CA, USA).

    Chromatin Immunoprecipitation:

    Article Title: Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection
    Article Snippet: RNA quantity and purity were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano Lab Chip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7.0. .. Approximately 10 µg total RNA, representing a specific adipose type, was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen, Carlsbad, CA, USA).

    RNA Extraction:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: Paragraph title: RNA extraction and library preparation ... Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: RNA Sequencing and Data Analysis To analyze the relationship between DNA methylation and gene expression, watermelon leaves were also collected for RNA extraction at 0 h, 48 h, and 25 d post-CGMMV inoculation. .. Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Agarose Gel Electrophoresis:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: After extraction, the concentration and purity of RNA were checked by an NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) at 260 nm; and the integrity of the total RNA was measured by 1.2% agarose gel electrophoresis and an Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: The total RNA was isolated from each sample using TRIzol® , and the degradation and contamination of RNA was assessed using agarose gel (Abcam, Cambridge, MA, USA) electrophoresis. .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Next-Generation Sequencing:

    Article Title: MicroRNA signatures from multidrug-resistant Mycobacterium tuberculosis
    Article Snippet: Paragraph title: Next-generation sequencing (NGS) ... Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

    Spectrophotometry:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: After extraction, the concentration and purity of RNA were checked by an NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) at 260 nm; and the integrity of the total RNA was measured by 1.2% agarose gel electrophoresis and an Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: RNA was checked on 1% agarose gels to avoid possible degradation and contamination, and was then examined by a Nano Photometer spectrophotometer (IMPLEN, CA, USA) for RNA purity. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

    Article Title: Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
    Article Snippet: Then the RNA integrity and purity were assessed using Agilent 2100 Bioanalyzer (Agilent, USA) and NanoDrop™ spectrophotometer (Thermo Fisher Scientific, USA), respectively. .. Briefly, Poly (A) mRNAs were captured from total RNA with poly-T oligo-attached magnetic beads (Thermo Fisher Scientific, USA), and then were fragmented into small pieces using divalent cations and heat treatments.

    DNA Methylation Assay:

    Article Title: DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber GreenMottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
    Article Snippet: RNA Sequencing and Data Analysis To analyze the relationship between DNA methylation and gene expression, watermelon leaves were also collected for RNA extraction at 0 h, 48 h, and 25 d post-CGMMV inoculation. .. Total RNA was subjected to Poly(A) mRNA isolation using poly-T oligo-attached magnetic beads (Invitrogen).

    Concentration Assay:

    Article Title: RNA-Seq transcriptome analysis of breast muscle in Pekin ducks supplemented with the dietary probiotic Clostridium butyricum
    Article Snippet: The high-quality RNA samples for library construction were selected according to 260/280 nm ratio (1.9–2.1), RNA concentration (≥ 500 ng/μL) and RNA integrity number (RIN ≥ 8.0), respectively (Additional file : Table S2). .. Briefly, the mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Invitrogen, USA) to pull down the poly-A mRNA and cleaved into short fragments of about 300 bases.

    Article Title: Elucidation of the molecular responses to waterlogging in Sesbania cannabina roots by transcriptome profiling
    Article Snippet: Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA) was used to measure RNA concentration, and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used to evaluate RNA integrity. .. In brief, mRNA was purified from 3 μg total RNA using poly-T oligo-attached magnetic beads (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer.

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    Thermo Fisher poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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