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    New England Biolabs poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 17 article reviews
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    poly t oligo attached magnetic beads - by Bioz Stars, 2020-04
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    Related Articles

    RNA Extraction:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Paragraph title: RNA extraction, library preparation and sequencing ... Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Paragraph title: RNA extraction, library preparation and sequencing ... Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×).

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Paragraph title: 2.2. RNA Extraction, Library Preparation, Sequencing and De Novo Assembly ... Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Total RNA extraction was performed using TRIzol (Invitrogen, 15596026). .. Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x).

    Amplification:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
    Article Snippet: Subsequently, mRNA was purified with poly-T oligo-attached magnetic beads, fragmented by First Strand Synthesis Reaction Buffer (NEBNext, MA, USA), and reverse transcribed into cDNA. .. Final cDNA fragments with lengths of 250–300 bp were amplified by polymerase chain reactions (PCR) and the PCR products were purified on an AMPure XP system (Beckman Coulter, USA).

    RNA Sequencing Assay:

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: Paragraph title: RNA sequencing ... The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x).

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: Paragraph title: Library preparation and RNA sequencing ... In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X).

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Generation and Analysis of mRNA Sequencing Data HeLa cells (designated as C), LD100-treated HeLa cells (L), SOD1 knockdown HeLa cells (S), DU145 cells (DC), LD100-treated DU145 cells (DL), RWPE-1 cells (RC), and LD100-treated RWPE-1 cells (RL) were used to construct RNA sequencing. .. Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x).

    Random Hexamer Labeling:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H) were used for first strand cDNA synthesis.

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA). .. First strand cDNA was synthesized using a random hexamer primer and M-MuLV reverse transcriptase.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H) were used for first strand cDNA synthesis.

    Article Title: Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander (Andrias davidianus)
    Article Snippet: Briefly: mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was transcribed using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H).

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H– ), and the second strand using DNA polymerase I and RNase H. The remaining overhangs were converted into blunt ends using exonuclease/polymerase, and after adenylation of the 3′ ends, ligated to NEBNext Adaptors with hairpin loop structure.

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. The first-strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H− ), and the second-strand cDNA was synthesized using DNA polymerase I and RNase H− .

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations. .. First-strand cDNA was synthesized using a random hexamer primer and M-MuLV Reverse Transcriptase (RNase H−).

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). .. Then, first strand cDNA was synthesized using M-MuLV Reverse Transcriptase(RNase H-)and random hexamer primers.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First, strand cDNA was produced based on the random hexamer primer and M-MuLV Reverse Transcriptase.

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x). .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H− ) were applied to the synthesis of first-strand cDNA, and DNA polymerase I and RNase H were applied to the synthesis of second-strand cDNA.

    Magnetic Beads:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H) were used for first strand cDNA synthesis.

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA). .. First strand cDNA was synthesized using a random hexamer primer and M-MuLV reverse transcriptase.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H) were used for first strand cDNA synthesis.

    Article Title: Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander (Andrias davidianus)
    Article Snippet: .. Briefly: mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was transcribed using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H).

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: .. Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H– ), and the second strand using DNA polymerase I and RNase H. The remaining overhangs were converted into blunt ends using exonuclease/polymerase, and after adenylation of the 3′ ends, ligated to NEBNext Adaptors with hairpin loop structure.

    Article Title: Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
    Article Snippet: .. Subsequently, mRNA was purified with poly-T oligo-attached magnetic beads, fragmented by First Strand Synthesis Reaction Buffer (NEBNext, MA, USA), and reverse transcribed into cDNA. .. After purification, end repair and 3′-end adenylation, the cDNA fragments were ligated to NEBNext Adaptor with a hairpin loop structure.

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: .. Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. The first-strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H− ), and the second-strand cDNA was synthesized using DNA polymerase I and RNase H− .

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: .. The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations. .. First-strand cDNA was synthesized using a random hexamer primer and M-MuLV Reverse Transcriptase (RNase H−).

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: .. In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). .. Then, first strand cDNA was synthesized using M-MuLV Reverse Transcriptase(RNase H-)and random hexamer primers.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: .. Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First, strand cDNA was produced based on the random hexamer primer and M-MuLV Reverse Transcriptase.

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: .. Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x). .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H− ) were applied to the synthesis of first-strand cDNA, and DNA polymerase I and RNase H were applied to the synthesis of second-strand cDNA.

    Isolation:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: RNA extraction, library preparation and sequencing Tilapia tissues of either liver or posterior intestine, at steady or different inflammational stages, were collected and the total RNA was isolated from each sample using Tiangen RNA prep Pure Plant Kit (Tiangen Biomart, Beijing). .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer.

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Paragraph title: RNA isolation, library construction and sequencing ... Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA).

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: RNA extraction, library preparation and sequencing Ramie samples at the different growth stages of H5 were collected and the total RNA was isolated from each sample using a Tiangen RNA prep Pure Plant Kit (Tiangen Biomart, Beijing). .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×).

    Article Title: Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
    Article Snippet: Transcriptome analysis Total RNA was isolated with TRIzol reagent (Invitrogen, UK) in accordance with the manufacturer’s instructions. .. Subsequently, mRNA was purified with poly-T oligo-attached magnetic beads, fragmented by First Strand Synthesis Reaction Buffer (NEBNext, MA, USA), and reverse transcribed into cDNA.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Total RNA was isolated from all the tissues collected above using the method described by Ghawana et al. [ ] and was detected using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE, USA) and the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Palo Alto, CA, USA). .. Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X).

    Ligation:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. These double-stranded cDNA fragments were end-repaired by adding a single ‘A’ base and ligation of adapters.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. These double-stranded cDNA fragments were end-repaired by adding a single ‘A’ base and ligation of adapters.

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. Blunt ends were generated, and then, A was added at the 3′ ends, followed by the ligation of NEBNext adaptors for hybridization.

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x). .. After adenylation of 3′ ends, NEBNext Adaptor with hairpin loop structure's ligation was carried out to prepare for hybridization.

    Sequencing:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Paragraph title: RNA extraction, library preparation and sequencing ... Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer.

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Paragraph title: RNA isolation, library construction and sequencing ... Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA).

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Paragraph title: RNA extraction, library preparation and sequencing ... Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×).

    Article Title: Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander (Andrias davidianus)
    Article Snippet: Paragraph title: Library preparation and transcriptome sequencing ... Briefly: mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: Paragraph title: Transcriptome Sequencing ... Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Paragraph title: Library preparation and sequencing ... Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×).

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The NEBNext Ultra RNA Library Prep Kit for Illumina sequencing (NEB, MA, USA) following the protocol as provided by the manufacturer was used for library preparation, index sequences were added to trace back the sequences to each sample. .. The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x).

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: Paragraph title: Library preparation for transcriptome sequencing ... Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations.

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: Library preparation and RNA sequencing In the present study, 3 μg of RNA per sample and the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) were used to generate the sequence libraries according to the manufacturer’s recommendations. .. In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X).

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Paragraph title: 2.2. RNA Extraction, Library Preparation, Sequencing and De Novo Assembly ... Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Paragraph title: 2.7. Generation and Analysis of mRNA Sequencing Data ... Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x).

    Generated:

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs, USA) following the manufacturer’s protocol. .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA).

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) according to the manufacturer’s instructions, and sample-specific index codes were added. .. Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. Blunt ends were generated, and then, A was added at the 3′ ends, followed by the ligation of NEBNext adaptors for hybridization.

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Then, 3 μ g RNA per sample was used as input material, and sequencing libraries were generated by NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, E7530S) according to the manufacturer's instructions. .. Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x).

    Spectrophotometry:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: RNA quality and quantity were determined by a Nano Photometer spectrophotometer (IMPLEN, CA, USA), a Qubit RNA Assay Kit in a Qubit 2.0 Flurometer (Life Technologies, CA, USA) and a Nano 6000 Assay Kit that was part of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: RNA quality and quantity were determined by a Nano Photometer spectrophotometer (IMPLEN, CA, USA), a Qubit RNA Assay Kit in a Qubit 2.0 Flurometer (Life Technologies, CA, USA) and a Nano 6000 Assay Kit that was part of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×).

    Article Title: Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
    Article Snippet: Then RNA purity and integrity were checked using NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively. .. Subsequently, mRNA was purified with poly-T oligo-attached magnetic beads, fragmented by First Strand Synthesis Reaction Buffer (NEBNext, MA, USA), and reverse transcribed into cDNA.

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The quality and integrity of the RNA samples was checked using the Nano 6000 Assay Kit of the Bioanalyzer 2100 (Agilent technologies, CA, USA) and the NanoPhotometer spectrophotometer (Implen, CA, USA). .. The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x).

    Purification:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H) were used for first strand cDNA synthesis.

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA). .. First strand cDNA was synthesized using a random hexamer primer and M-MuLV reverse transcriptase.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. Random hexamer primer and M-MuLV Reverse Transcriptase (RNase H) were used for first strand cDNA synthesis.

    Article Title: Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander (Andrias davidianus)
    Article Snippet: .. Briefly: mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was transcribed using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H).

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: .. Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H– ), and the second strand using DNA polymerase I and RNase H. The remaining overhangs were converted into blunt ends using exonuclease/polymerase, and after adenylation of the 3′ ends, ligated to NEBNext Adaptors with hairpin loop structure.

    Article Title: Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
    Article Snippet: .. Subsequently, mRNA was purified with poly-T oligo-attached magnetic beads, fragmented by First Strand Synthesis Reaction Buffer (NEBNext, MA, USA), and reverse transcribed into cDNA. .. After purification, end repair and 3′-end adenylation, the cDNA fragments were ligated to NEBNext Adaptor with a hairpin loop structure.

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: .. Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. The first-strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H− ), and the second-strand cDNA was synthesized using DNA polymerase I and RNase H− .

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: .. The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations. .. First-strand cDNA was synthesized using a random hexamer primer and M-MuLV Reverse Transcriptase (RNase H−).

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: .. In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). .. Then, first strand cDNA was synthesized using M-MuLV Reverse Transcriptase(RNase H-)and random hexamer primers.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: .. Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First, strand cDNA was produced based on the random hexamer primer and M-MuLV Reverse Transcriptase.

    Produced:

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. First, strand cDNA was produced based on the random hexamer primer and M-MuLV Reverse Transcriptase.

    Polymerase Chain Reaction:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA). .. 3 μl USER enzyme (New England Biolabs, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95°C prior to PCR using phusion high-fidelity DNA polymerase, universal PCR primers and index (X) primer.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
    Article Snippet: Subsequently, mRNA was purified with poly-T oligo-attached magnetic beads, fragmented by First Strand Synthesis Reaction Buffer (NEBNext, MA, USA), and reverse transcribed into cDNA. .. Final cDNA fragments with lengths of 250–300 bp were amplified by polymerase chain reactions (PCR) and the PCR products were purified on an AMPure XP system (Beckman Coulter, USA).

    Incubation:

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X). .. The size-selected, adaptor-ligated cDNA was incubated with 3 μl uracil-specific extension reagent (USER) enzyme (NEB, United States) at 37°C for 15 min, followed by 5 min at 95°C.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. The fragments in library were purified with AMPure XP system (Beckman Coulter, Danvers, MA, USA) to firstly select for 240 bp cDNA fragments; 3 μL USER Enzyme (New England Biolabs) was incubated with size-selected, adaptor-ligated cDNA for 15 min at 37 °C and then 5 min at 95 °C.

    cDNA Library Assay:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Construct:

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Twenty micrograms of total RNA from each sample was sent to Novogene Bioinformatics Technology Co. Ltd (Beijing), where the libraries were constructed, and sequenced using the Illumina HiSeq 2000 platform. .. Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×).

    Article Title: Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander (Andrias davidianus)
    Article Snippet: Library preparation and transcriptome sequencing Sequencing libraries were constructed using 3 μg of RNA per sample using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA), following the manufacturer’s instructions. .. Briefly: mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Library preparation and sequencing For each sample, we purified RNA from both brain and gonad tissues to construct 2 sequencing libraries. .. Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×).

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: Library preparation for transcriptome sequencing The sequencing cDNA libraries were constructed for C .humilis and C .glandulosa respectively with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations. .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Sequencing libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA). .. Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Generation and Analysis of mRNA Sequencing Data HeLa cells (designated as C), LD100-treated HeLa cells (L), SOD1 knockdown HeLa cells (S), DU145 cells (DC), LD100-treated DU145 cells (DL), RWPE-1 cells (RC), and LD100-treated RWPE-1 cells (RL) were used to construct RNA sequencing. .. Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x).

    Modification:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Gel Purification:

    Article Title: Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer. .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Article Title: Transcript Profiling Reveals Auxin and Cytokinin Signaling Pathways and Transcription Regulation during In Vitro Organogenesis of Ramie (Boehmeria nivea L. Gaud)
    Article Snippet: Briefly, the mRNA was purified by poly-T oligo-attached magnetic beads and fragmented by divalent cations under elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×). .. The adaptor modified fragments were selected by gel purification and amplified, through PCR, to create the final cDNA library.

    Synthesized:

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA). .. First strand cDNA was synthesized using a random hexamer primer and M-MuLV reverse transcriptase.

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X). .. First strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H– ), and the second strand using DNA polymerase I and RNase H. The remaining overhangs were converted into blunt ends using exonuclease/polymerase, and after adenylation of the 3′ ends, ligated to NEBNext Adaptors with hairpin loop structure.

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. The first-strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H− ), and the second-strand cDNA was synthesized using DNA polymerase I and RNase H− .

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x). .. Synthesis of first strand cDNA was performed using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H− ), subsequently second strand cDNA was synthesized using DNA polymerase I and RNase H. After terminal repair of the sequences, the 3′ ends were adenylated and NEBnext adapters (NEB, MA, USA) were ligated.

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations. .. First-strand cDNA was synthesized using a random hexamer primer and M-MuLV Reverse Transcriptase (RNase H−).

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). .. Then, first strand cDNA was synthesized using M-MuLV Reverse Transcriptase(RNase H-)and random hexamer primers.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. Immediately after, a second strand cDNA was synthesized using DNA polymerase I and RNase H. After adenylation reaction of 3′ ends of DNA fragments, NEBNext Adaptor containing hairpin loop structure were ligated for hybridization.

    Concentration Assay:

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: RNA isolation, library construction and sequencing RNA was isolated using Trizol reagent (Invitrogen, USA) according the manufacturer’s protocol, concentration was measured by the Qubit RNA assay kit (Life Technologies, USA), and integrity was assessed with the RNA nano 6000 assay kit (Agilent Technologies, USA). .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA).

    Article Title: Dynamic Changes in the Microbiome and Mucosal Immune Microenvironment of the Lower Respiratory Tract by Influenza Virus Infection
    Article Snippet: The concentration of RNA was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, Camarillo, CA, United States), and its integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States). .. Briefly, the mRNAs were purified from total RNA using poly-T oligo-attached magnetic beads, and fragmented using divalent cations at high temperature in the NEBNext First Strand Synthesis Reaction Buffer (5X).

    Article Title: An influential meal: host plant dependent transcriptional variation in the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae)
    Article Snippet: The RNA concentration was measured using the Qubit RNA Assay Kit on a Qubit 2.0 Fluorometer (Invitrogen Co. Ltd., San Diego, USA). .. The mRNA was purified using poly-T oligo-attached magnetic beads followed by fragmentation using NEBNext First Strand Synthesis Reaction Buffer (5x).

    Hybridization:

    Article Title: Global gene expression patterns of grass carp following compensatory growth
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented by NEBNext first strand synthesis reaction buffer (New England Biolabs, USA). .. Second strand cDNA synthesis was subsequently performed using DNA polymerase I and RNase H. After adenylation of the 3’ end of DNA fragments, NEBNext adaptors with hairpin loop structure were ligated in preparation for hybridization.

    Article Title: Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander (Andrias davidianus)
    Article Snippet: Briefly: mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. After adenylation of 3′ ends of DNA fragments, NEBNext adaptors with hairpin loop structure were ligated to prepare for hybridization.

    Article Title: Transcriptomic profiling reveals molecular regulation of seasonal reproduction in Tibetan highland fish, Gymnocypris przewalskii
    Article Snippet: Briefly, mRNAs were purified from total RNAs using poly-T oligo-attached magnetic beads, which were fragmented using divalent cations in NEBNext First Strand Synthesis Reaction Buffer (5×). .. Blunt ends were generated, and then, A was added at the 3′ ends, followed by the ligation of NEBNext adaptors for hybridization.

    Article Title: Comparison of fruit organic acids and metabolism-related gene expression between Cerasus humilis (Bge.) Sok and Cerasus glandulosa (Thunb.) Lois
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and then fragmented by heating in 5X NEBNext First Strand Synthesis Reaction Buffer containing divalent cations. .. After adenylating the 3′ ends of cDNA fragments, the NEBNext Adaptor with a hairpin loop structure was ligated for a subsequent hybridization.

    Article Title: Transcriptional profiling of two different physiological states of the yak mammary gland using RNA sequencing
    Article Snippet: In brief, after purification from total RNA using poly-T oligo-attached magnetic beads, mRNA was split into fragments using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). .. After adenylation of the 3’ ends of DNA fragments, NEBNext Adaptors with a hairpin loop structure were ligated for further hybridization.

    Article Title: De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese Endemic Acer miaotaiense (Aceraceae)
    Article Snippet: Briefly, purified mRNA was obtained from total RNA (1 µg) per sample using poly-T oligo-attached magnetic beads; then, the mRNA was fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). .. Immediately after, a second strand cDNA was synthesized using DNA polymerase I and RNase H. After adenylation reaction of 3′ ends of DNA fragments, NEBNext Adaptor containing hairpin loop structure were ligated for hybridization.

    Article Title: The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network
    Article Snippet: Briefly, after purifying mRNA from total RNA by poly-T oligo-attached magnetic beads, fragmentation of mRNA was carried out at elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5x). .. After adenylation of 3′ ends, NEBNext Adaptor with hairpin loop structure's ligation was carried out to prepare for hybridization.

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    New England Biolabs poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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