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Illumina Inc poly t oligo attached magnetic beads
Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 34 article reviews
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poly t oligo attached magnetic beads - by Bioz Stars, 2020-04
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Nucleic Acid Electrophoresis:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: RNA quantity and quality were assessed by gel electrophoresis and spectrophotometry, respectively. .. Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. The quantity and quality of RNA were assessed by gel electrophoresis and spectrophotometry.

RNA Extraction:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: Paragraph title: Insect materials, RNA extraction, cDNA library construction, and Illumina sequencing ... Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: Several batches of five to ten larvae in late third to early fourth stage were used for RNA extraction respectively, using the Arcturus Picopure RNA Extraction Kit (Arcturus, California, USA). .. Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification.

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: Paragraph title: 4.2. RNA Extraction, Reverse Transcription and Sequencing ... After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA).

Amplification:

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. .. The unsuitable fragments were removed using AMPureXP beads (Beckman Coulter Inc, Brae, CA, USA), and the sequencing library was constructed with PCR amplification.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. After end repair process, cDNA fragments were linked with adaptors and amplified by PCR.

RNA Sequencing Assay:

Article Title: De Novo Transcriptome Analysis of an Aerial Microalga Trentepohlia jolithus: Pathway Description and Gene Discovery for Carbon Fixation and Carotenoid Biosynthesis
Article Snippet: .. RNA-Seq Library Preparation and Sequencing Four µg of the total RNA sample were used to isolate poly-A containing mRNA molecules, using poly-T oligo-attached magnetic beads (Illumina). .. Then, the poly-A RNA was purified and fragmented into smaller pieces (200–700 nt) using divalent cations at 94°C for exactly 5 minutes.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Paragraph title: Library construction and Illumina RNA-seq ... Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA.

Article Title: Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to instructions. .. Briefly, the mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and short fragments were obtained under elevated temperature by adding an Illumina proprietary fragmentation buffer.

Random Hexamer Labeling:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA. .. Then a SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, Camarillo, CA) was employed for double-stranded cDNA synthesis with random hexamer (N6) primers (Illumina, San Diego, CA, USA).

Article Title: Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress
Article Snippet: Briefly, the mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and short fragments were obtained under elevated temperature by adding an Illumina proprietary fragmentation buffer. .. First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Magnetic Beads:

Article Title: De Novo Transcriptome Analysis of an Aerial Microalga Trentepohlia jolithus: Pathway Description and Gene Discovery for Carbon Fixation and Carotenoid Biosynthesis
Article Snippet: .. RNA-Seq Library Preparation and Sequencing Four µg of the total RNA sample were used to isolate poly-A containing mRNA molecules, using poly-T oligo-attached magnetic beads (Illumina). .. Then, the poly-A RNA was purified and fragmented into smaller pieces (200–700 nt) using divalent cations at 94°C for exactly 5 minutes.

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: .. Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA. .. Then a SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, Camarillo, CA) was employed for double-stranded cDNA synthesis with random hexamer (N6) primers (Illumina, San Diego, CA, USA).

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. ..

Article Title: Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System
Article Snippet: .. Total RNA was extracted using the Qiagen RNeasy Mini Kit, per manufacturer recommended protocol. mRNA purification utilized a poly-T oligo-attached magnetic beads, Conversion into cDNA done using the mRNA sequencing preparation kit from Illumina (part number 1004898). .. Sequencing of the cDNA was performed on an Illumina GAIIx sequencing system.

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: .. Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification. ..

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: .. After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. The quantity and quality of RNA were assessed by gel electrophoresis and spectrophotometry.

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification. ..

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: Genome-wide Studies of Copy Number Variation and Exome Sequencing Identify Rare Variants in BAG3 as a Cause of Dilated Cardiomyopathy
Article Snippet: .. Poly-A containing mRNA was isolated by poly-T oligo-attached magnetic beads. cDNA libraries were prepared with Illumina's mRNA-Seq Sample Prep kit according to the manufacturer's instructions. .. Briefly, mRNA was fragmented into small pieces (∼200 bp) with divalent cations in the fragmentation buffer at 94°C for 5 min. Cleaved RNA fragments were transcribed into first-strand cDNA with SuperScript II (Invitrogen) reverse transcriptase and random primers.

Isolation:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: The total RNA of R . padi was isolated using TRIzol reagent (Tiangen Biotech, Beijing, China) with minor modifications at the recovery step, in which RNase-free filter columns (Sangon Biotech, Shanghai, China) were used. .. Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, total RNA from FW PVCs, FW MRCs, SW PVCs and SW MRCs (each n = 2) was extracted using the mirVanaTM miRNA isolation kit (Applied Biosystems). .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ).

Article Title: Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress
Article Snippet: Paragraph title: RNA isolation, library preparation, and sequencing ... Briefly, the mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and short fragments were obtained under elevated temperature by adding an Illumina proprietary fragmentation buffer.

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: .. After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. The quantity and quality of RNA were assessed by gel electrophoresis and spectrophotometry.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Genome-wide Studies of Copy Number Variation and Exome Sequencing Identify Rare Variants in BAG3 as a Cause of Dilated Cardiomyopathy
Article Snippet: .. Poly-A containing mRNA was isolated by poly-T oligo-attached magnetic beads. cDNA libraries were prepared with Illumina's mRNA-Seq Sample Prep kit according to the manufacturer's instructions. .. Briefly, mRNA was fragmented into small pieces (∼200 bp) with divalent cations in the fragmentation buffer at 94°C for 5 min. Cleaved RNA fragments were transcribed into first-strand cDNA with SuperScript II (Invitrogen) reverse transcriptase and random primers.

Synthesized:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA. .. The T4 DNA polymerase, Klenow DNA polymerase and T4 polynucleotide kinase were used for end-repair and phosphorylation of synthesized cDNA.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification. .. The RNA template was then removed and a replacement strand synthesized to generate double-stranded cDNA.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Sequencing:

Article Title: De Novo Transcriptome Analysis of an Aerial Microalga Trentepohlia jolithus: Pathway Description and Gene Discovery for Carbon Fixation and Carotenoid Biosynthesis
Article Snippet: .. RNA-Seq Library Preparation and Sequencing Four µg of the total RNA sample were used to isolate poly-A containing mRNA molecules, using poly-T oligo-attached magnetic beads (Illumina). .. Then, the poly-A RNA was purified and fragmented into smaller pieces (200–700 nt) using divalent cations at 94°C for exactly 5 minutes.

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: Paragraph title: Insect materials, RNA extraction, cDNA library construction, and Illumina sequencing ... Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Paragraph title: Library construction and sequencing ... Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System
Article Snippet: .. Total RNA was extracted using the Qiagen RNeasy Mini Kit, per manufacturer recommended protocol. mRNA purification utilized a poly-T oligo-attached magnetic beads, Conversion into cDNA done using the mRNA sequencing preparation kit from Illumina (part number 1004898). .. Sequencing of the cDNA was performed on an Illumina GAIIx sequencing system.

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: C) for Illumina Paired-End Indexed Sequencing http://www.biotech.wisc.edu/Libraries/GEC_documents/TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf . .. Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification.

Article Title: Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress
Article Snippet: Paragraph title: RNA isolation, library preparation, and sequencing ... Briefly, the mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and short fragments were obtained under elevated temperature by adding an Illumina proprietary fragmentation buffer.

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: Paragraph title: 4.2. RNA Extraction, Reverse Transcription and Sequencing ... After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA).

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: The stranded total RNA libraries were prepared in accordance to the Illumina TruSeq Stranded mRNA Sample Preparation (October 2013 Rev E.) for Illumina Paired-End Multiplexed Sequencing. .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification.

Article Title: Genome-wide Studies of Copy Number Variation and Exome Sequencing Identify Rare Variants in BAG3 as a Cause of Dilated Cardiomyopathy
Article Snippet: Paragraph title: Transcriptome Sequencing ... Poly-A containing mRNA was isolated by poly-T oligo-attached magnetic beads. cDNA libraries were prepared with Illumina's mRNA-Seq Sample Prep kit according to the manufacturer's instructions.

Ligation:

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification. .. A single “A” nucleotide is added to the 3′ end of the blunt fragments to prevent ligating to one another during adapter ligation reaction.

Spectrophotometry:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: RNA quantity and quality were assessed by gel electrophoresis and spectrophotometry, respectively. .. Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. The quantity and quality of RNA were assessed by gel electrophoresis and spectrophotometry.

Purification:

Article Title: De Novo Transcriptome Analysis of an Aerial Microalga Trentepohlia jolithus: Pathway Description and Gene Discovery for Carbon Fixation and Carotenoid Biosynthesis
Article Snippet: RNA-Seq Library Preparation and Sequencing Four µg of the total RNA sample were used to isolate poly-A containing mRNA molecules, using poly-T oligo-attached magnetic beads (Illumina). .. Then, the poly-A RNA was purified and fragmented into smaller pieces (200–700 nt) using divalent cations at 94°C for exactly 5 minutes.

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: Around 20 μg purified RNA samples were sent to Beijing Genomics Institute (BGI) (Shenzhen, China) for cDNA library construction. .. Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. ..

Article Title: Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System
Article Snippet: .. Total RNA was extracted using the Qiagen RNeasy Mini Kit, per manufacturer recommended protocol. mRNA purification utilized a poly-T oligo-attached magnetic beads, Conversion into cDNA done using the mRNA sequencing preparation kit from Illumina (part number 1004898). .. Sequencing of the cDNA was performed on an Illumina GAIIx sequencing system.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: .. For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. The purified mRNA was cut into fragments with an average length of 155 bp using divalent cations at 95°C for 8 min.

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: .. Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification. ..

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. Purified RNA was used to construct a directional cDNA library using the cDNA Synthesis Kit (Illumina), and then the cDNA library was sequenced using a HiSeq 2000 (Illumina) to obtain short sequences.

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification. ..

Article Title: Genome-wide Studies of Copy Number Variation and Exome Sequencing Identify Rare Variants in BAG3 as a Cause of Dilated Cardiomyopathy
Article Snippet: RNA was extracted from frozen left ventricular myocardium of two normal and two DCM hearts with Trizol reagent and purified with QIAGEN RNeasy columns and treated with 20 U DNase with RNase inhibitor. .. Poly-A containing mRNA was isolated by poly-T oligo-attached magnetic beads. cDNA libraries were prepared with Illumina's mRNA-Seq Sample Prep kit according to the manufacturer's instructions.

Real-time Polymerase Chain Reaction:

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: RNA was treated with DNAse I (RNase–Free DNase Set Qiagen) to remove genomic DNA contamination and subsequently used for cDNA synthesis, either using Superscript III reverse transcriptase and Oligo-dT 20 primers (Invitrogen) (qPCR), or using Mint-Universal cDNA Synthesis kit. .. Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification.

Polymerase Chain Reaction:

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. DNA fragments that ligated with adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycles PCR reaction.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. .. The unsuitable fragments were removed using AMPureXP beads (Beckman Coulter Inc, Brae, CA, USA), and the sequencing library was constructed with PCR amplification.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ). .. After end repair process, cDNA fragments were linked with adaptors and amplified by PCR.

Generated:

Article Title: Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to instructions. .. Briefly, the mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and short fragments were obtained under elevated temperature by adding an Illumina proprietary fragmentation buffer.

Construct:

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Library construction and sequencing After validating the integrity and purity of total RNA, cDNA library was constructed according to the protocol of TruSeq RNA Sample Prep Preparation Kit (RS-930-2001; Illumina, San Diego, CA). .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. Purified RNA was used to construct a directional cDNA library using the cDNA Synthesis Kit (Illumina), and then the cDNA library was sequenced using a HiSeq 2000 (Illumina) to obtain short sequences.

Clone Assay:

Article Title: Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System
Article Snippet: Paragraph title: Cloning and purification of insect nucleic acids ... Total RNA was extracted using the Qiagen RNeasy Mini Kit, per manufacturer recommended protocol. mRNA purification utilized a poly-T oligo-attached magnetic beads, Conversion into cDNA done using the mRNA sequencing preparation kit from Illumina (part number 1004898).

cDNA Library Assay:

Article Title: De novo transcriptome analysis and microsatellite marker development for population genetic study of a serious insect pest, Rhopalosiphum padi (L.) (Hemiptera: Aphididae)
Article Snippet: Paragraph title: Insect materials, RNA extraction, cDNA library construction, and Illumina sequencing ... Briefly, poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA) were used to isolate poly (A) RNA from total RNA.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Library construction and sequencing After validating the integrity and purity of total RNA, cDNA library was constructed according to the protocol of TruSeq RNA Sample Prep Preparation Kit (RS-930-2001; Illumina, San Diego, CA). .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... For library construction, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Illumina) ( ).

Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)
Article Snippet: After mixing an approximately equal weight of mixture for each species, total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer instructions, then poly-A mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads (Illumina Inc., San Diego, CA, USA). .. Purified RNA was used to construct a directional cDNA library using the cDNA Synthesis Kit (Illumina), and then the cDNA library was sequenced using a HiSeq 2000 (Illumina) to obtain short sequences.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Agarose Gel Electrophoresis:

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: RNA quality was verified using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and checked by RNase-free agarose gel electrophoresis. .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Sample Prep:

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Library construction and sequencing After validating the integrity and purity of total RNA, cDNA library was constructed according to the protocol of TruSeq RNA Sample Prep Preparation Kit (RS-930-2001; Illumina, San Diego, CA). .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Transcriptome Profiling and Genetic Study Reveal Amplified Carboxylesterase Genes Implicated in Temephos Resistance, in the Asian Tiger Mosquito Aedes albopictus
Article Snippet: For Illumina sequencing, libraries were prepared in accordance with the Illumina Tru Seq RNA sample preparation guide (May 2012, rev. .. Briefly, poly-A mRNA were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification.

Article Title: Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to instructions. .. Briefly, the mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and short fragments were obtained under elevated temperature by adding an Illumina proprietary fragmentation buffer.

Article Title: An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis
Article Snippet: The stranded total RNA libraries were prepared in accordance to the Illumina TruSeq Stranded mRNA Sample Preparation (October 2013 Rev E.) for Illumina Paired-End Multiplexed Sequencing. .. Poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and 2 rounds of purification.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: A cDNA library of each pooled RNA was obtained using a TruSeq RNA Sample preparation kit (Illumina, USA), according to the manufacturer's instructions. .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: Genome-wide Studies of Copy Number Variation and Exome Sequencing Identify Rare Variants in BAG3 as a Cause of Dilated Cardiomyopathy
Article Snippet: .. Poly-A containing mRNA was isolated by poly-T oligo-attached magnetic beads. cDNA libraries were prepared with Illumina's mRNA-Seq Sample Prep kit according to the manufacturer's instructions. .. Briefly, mRNA was fragmented into small pieces (∼200 bp) with divalent cations in the fragmentation buffer at 94°C for 5 min. Cleaved RNA fragments were transcribed into first-strand cDNA with SuperScript II (Invitrogen) reverse transcriptase and random primers.

Concentration Assay:

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. .. After quantified using the Quant-iT PicoGreen ds DNA Assay Kit (Invitrogen, Eugene, OR, USA) and fluorospectrophotometry and quantified with Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany), the multiplexed DNA libraries were mixed by equal volume with normalized 10 nM concentration.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: The concentration of the total RNA was further quantified using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, Delaware USA). .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

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    Illumina Inc poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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