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Illumina Inc poly t oligo attached magnetic beads
Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Illumina Inc
Average 99 stars, based on 33 article reviews
Price from $9.99 to $1999.99
poly t oligo attached magnetic beads - by Bioz Stars, 2020-01
99/100 stars

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RNA Extraction:

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: Paragraph title: 4.4. RNA Extraction, Illumina Sequencing, and Data Processing ... First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA.

Amplification:

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. .. The unsuitable fragments were removed using AMPureXP beads (Beckman Coulter Inc, Brae, CA, USA), and the sequencing library was constructed with PCR amplification.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt). .. After that, the libraries were end-repaired and ligated with adaptors, and amplified with Phusion DNA polymerase.

Sample Prep:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s recommendations. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Library construction and sequencing After validating the integrity and purity of total RNA, cDNA library was constructed according to the protocol of TruSeq RNA Sample Prep Preparation Kit (RS-930-2001; Illumina, San Diego, CA). .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: The cDNA library for Illumina sequencing was prepared by using a TruseqTM RNA sample prep kit (Illumina). .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: A cDNA library of each pooled RNA was obtained using a TruSeq RNA Sample preparation kit (Illumina, USA), according to the manufacturer's instructions. .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Magnetic Beads:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). ..

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: .. First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA. .. The purified mRNA was then fragmented into 200- to 700-nt pieces.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. ..

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt). .. The first and second strands of cDNA were synthesized by SuperScript II reverse transcriptase and DNA polymerase I, respectively.

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: .. The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ). .. The poly(A) RNA was then fragmented into smaller pieces using divalent cations at 94 °C for 4 min. First-strand cDNA was synthesized using SuperScript II reverse transcriptase and random primers.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing
Article Snippet: .. First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly (A)+ RNA from the total RNA quantified. .. Subsequently, poly (A)+ RNA was purified and fragmented into smaller pieces (200–700 nt) using divalent cations at 94°C for exactly 5 minutes.

Isolation:

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, total RNA from FW PVCs, FW MRCs, SW PVCs and SW MRCs (each n = 2) was extracted using the mirVanaTM miRNA isolation kit (Applied Biosystems). .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Biomarker Assay:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Library construction and RNA-Seq Construction of the four libraries and the RNA-Seq were performed by the Biomarker Biotechnology Corporation (Beijing, China). .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Synthesized:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. Double-stranded cDNA was synthesized using SuperScript II, buffer, dNTPs, RNaseH, DNA polymerase I, and random hexamer primers (Illumina).

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA. .. The first strand of cDNA was synthesized using random hexamer primers, followed by synthesis of the second strand using SuperScript Double-Stranded cDNA Synthesis kit reagents (Invitrogen, Camarillo, CA, USA).

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt). .. The first and second strands of cDNA were synthesized by SuperScript II reverse transcriptase and DNA polymerase I, respectively.

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ). .. The poly(A) RNA was then fragmented into smaller pieces using divalent cations at 94 °C for 4 min. First-strand cDNA was synthesized using SuperScript II reverse transcriptase and random primers.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Article Title: De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing
Article Snippet: First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly (A)+ RNA from the total RNA quantified. .. First-strand cDNA was synthesized with SuperScript II reverse transcriptase and random primers using the small fragment RNAs as templates.

RNA Sequencing Assay:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Paragraph title: Library construction and RNA-Seq ... Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Paragraph title: Library construction and Illumina RNA-seq ... Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: Paragraph title: Library Construction and RNA-seq ... Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Generated:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: RNA sequencing libraries were generated using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s recommendations. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Construct:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Five micrograms of total RNA from each sample (EM, EH, LM, and LH) were used to construct the sequencing libraries. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Library construction and sequencing After validating the integrity and purity of total RNA, cDNA library was constructed according to the protocol of TruSeq RNA Sample Prep Preparation Kit (RS-930-2001; Illumina, San Diego, CA). .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: cDNA library establishment and sequencing The cDNA library was constructed using the NEBNext Ultra RNA Library Prep Kit (NEB, Ipswich, USA) according to the manufacturer’s instructions [ ]. .. The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ).

Purification:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). ..

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA. .. The purified mRNA was then fragmented into 200- to 700-nt pieces.

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: .. Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. Then the mRNA was fragmented by using divalent cations in Illumina proprietary fragmentation buffer at 94 °C for 1 min. First strand cDNAs were synthesized using random oligonucleotides and SuperScript II, then second cDNAs were synthesized using DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase, followed by 3’ end adenylation.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. ..

Article Title: De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing
Article Snippet: First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly (A)+ RNA from the total RNA quantified. .. Subsequently, poly (A)+ RNA was purified and fragmented into smaller pieces (200–700 nt) using divalent cations at 94°C for exactly 5 minutes.

Sequencing:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Five micrograms of total RNA from each sample (EM, EH, LM, and LH) were used to construct the sequencing libraries. .. Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA).

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: Paragraph title: 4.4. RNA Extraction, Illumina Sequencing, and Data Processing ... First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Paragraph title: Library construction and sequencing ... Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: The cDNA library for Illumina sequencing was prepared by using a TruseqTM RNA sample prep kit (Illumina). .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: Paragraph title: cDNA library establishment and sequencing ... The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ).

Article Title: De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing
Article Snippet: Paragraph title: Preparation of cDNA library for transcriptome sequencing ... First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly (A)+ RNA from the total RNA quantified.

Polymerase Chain Reaction:

Article Title: Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel
Article Snippet: Briefly, 8 cDNA libraries were constructed (2 X 4 groups), each prepared from 300 ng of total RNA. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA) to remove the ribosomal RNA. .. DNA fragments that ligated with adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycles PCR reaction.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. .. The unsuitable fragments were removed using AMPureXP beads (Beckman Coulter Inc, Brae, CA, USA), and the sequencing library was constructed with PCR amplification.

Random Hexamer Labeling:

Article Title: De novo sequencing and comparative transcriptome analysis of the male and hermaphroditic flowers provide insights into the regulation of flower formation in andromonoecious taihangia rupestris
Article Snippet: Briefly, the poly (A)-containing mRNA molecules were purified from the total RNA by using the poly-T oligo-attached magnetic beads (Illumina, San Diego, CA, USA). .. Double-stranded cDNA was synthesized using SuperScript II, buffer, dNTPs, RNaseH, DNA polymerase I, and random hexamer primers (Illumina).

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA. .. The first strand of cDNA was synthesized using random hexamer primers, followed by synthesis of the second strand using SuperScript Double-Stranded cDNA Synthesis kit reagents (Invitrogen, Camarillo, CA, USA).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA. .. First-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and reverse transcriptase (Invitrogen, USA).

Spectrophotometry:

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: RNA was then quantified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop, Wilmington, DE, USA). .. First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA.

cDNA Library Assay:

Article Title: Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.)
Article Snippet: Total RNA (10 μg) was prepared for the cDNA library for each pool. .. First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly(A) mRNA from total RNA.

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Library construction and sequencing After validating the integrity and purity of total RNA, cDNA library was constructed according to the protocol of TruSeq RNA Sample Prep Preparation Kit (RS-930-2001; Illumina, San Diego, CA). .. Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature.

Article Title: Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis
Article Snippet: The cDNA library for Illumina sequencing was prepared by using a TruseqTM RNA sample prep kit (Illumina). .. Briefly, mRNA was first enriched by using poly-T oligo-attached magnetic beads (Illumina), which was subsequently broken down into fragments (200–700 nt).

Article Title: De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development
Article Snippet: Paragraph title: cDNA library establishment and sequencing ... The total RNA was used to isolate poly(A) RNA using poly-T oligo-attached magnetic beads (Illumina , San Diego, CA, USA ).

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: Paragraph title: RNA isolation and cDNA library construction ... Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Article Title: De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing
Article Snippet: Paragraph title: Preparation of cDNA library for transcriptome sequencing ... First, poly-T oligo-attached magnetic beads (Illumina) were used to isolate poly (A)+ RNA from the total RNA quantified.

Agarose Gel Electrophoresis:

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: RNA quality was verified using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and checked by RNase-free agarose gel electrophoresis. .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

Concentration Assay:

Article Title: Genome-Wide Identification of Small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and Their Regulation Role in the Adaption to Gastrointestinal Environment
Article Snippet: Briefly, the poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (Illumina, San Diego, CA) from total RNA, and sheared to small fragments using divalent cations under elevated temperature. .. After quantified using the Quant-iT PicoGreen ds DNA Assay Kit (Invitrogen, Eugene, OR, USA) and fluorospectrophotometry and quantified with Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany), the multiplexed DNA libraries were mixed by equal volume with normalized 10 nM concentration.

Article Title: Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
Article Snippet: The concentration of the total RNA was further quantified using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, Delaware USA). .. Poly-T oligo-attached magnetic beads (Illumina Inc., San Diego USA) were used to isolate poly-A mRNA from total RNA.

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    Illumina Inc poly t oligo attached magnetic beads
    Poly T Oligo Attached Magnetic Beads, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo attached magnetic beads/product/Illumina Inc
    Average 99 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    poly t oligo attached magnetic beads - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

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