poly dt oligos  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly dt oligos
    Poly Dt Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly dt oligos/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    poly dt oligos - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ]. .. The cDNAs were then blunt-end ligated to EcoR I adapters, digested with Not I and directionally cloned into a phagemid vector pT7T3-Pac.

    Amplification:

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data. .. For RT-PCR analysis of the TMV-U1 infection, primer SP6289 was used for the synthesis of first strand cDNA followed by PCR amplification of the movement protein region of TMV-U1.

    Article Title: Genetic transformation of Vitis vinifera via organogenesis
    Article Snippet: Poly(A+ )RNA was isolated from total RNA using oligo d(T) Dynabeads (Dynal) following the manufacturer's protocol. .. First strand cDNA (30–50 ng) was amplified with the forward primer 5'-CTTTGGAACTCGTGTTGAGCTCTCA-3' (corresponding to the DefH9 ULR region +89 +113, with +1 the transcription initiation nucleotide).

    Synthesized:

    Article Title: Deficiency in the nuclear factor E2-related factor 2 renders pancreatic ?-cells vulnerable to arsenic-induced cell damage
    Article Snippet: Total RNA was reverse transcribed with MuLV reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA). .. The primers were designed using Primer Express 4 (Applied Biosystems) and synthesized by Bioneer Inc. (Alameda, CA).

    Article Title: Degrading products of chondroitin sulfate can induce hypertrophy-like changes and MMP-13/ADAMTS5 production in chondrocytes
    Article Snippet: .. The cDNA was synthesized from 2 μg of total RNA using reverse transcription kit (ELPIS Biotech, Korea) with random hexameter and oligo d(T) primer, and real-time qPCR was performed using the SYBR green master mix on the ViiA7 machine (Applied Biosystems, Waltham, MA). .. The expression of target genes was normalized to Gapdh.

    Quantitative RT-PCR:

    Article Title: Deficiency in the nuclear factor E2-related factor 2 renders pancreatic ?-cells vulnerable to arsenic-induced cell damage
    Article Snippet: Paragraph title: Reverse transcription quantitative real-time PCR (RT-qPCR) ... Total RNA was reverse transcribed with MuLV reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA).

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: Paragraph title: RT-qPCR and RT-PCR ... First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data.

    SYBR Green Assay:

    Article Title: Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
    Article Snippet: First strand cDNA syntheses were performed in a 20 μl reaction volume with 3 μg of total RNA, using oligo(dT) primers (Invitrogen, Carlsbad, CA, USA) and 200 U of Superscript Reverse Transcriptase II (Invitrogen). .. Amplifications were performed with SYBR Green PCR mastermix and analysed using the ABI PRISM 7700 sequence detection system following the manufacturer's instructions (Applied Biosystems, Foster City, CA, USA).

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: .. First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data. ..

    Article Title: Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
    Article Snippet: Measurement of RNA Expression by qPCR To measure RNA transcription, the total unamplified RNA was DNase I treated (Ambion, Austin, USA) and reverse transcribed using oligo-d(T), dNTP, and M-MLV Reverse Transcriptase (all Invitrogen, Glostrup, Denmark). .. The cDNA was subjected to qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA, USA).

    Article Title: Degrading products of chondroitin sulfate can induce hypertrophy-like changes and MMP-13/ADAMTS5 production in chondrocytes
    Article Snippet: .. The cDNA was synthesized from 2 μg of total RNA using reverse transcription kit (ELPIS Biotech, Korea) with random hexameter and oligo d(T) primer, and real-time qPCR was performed using the SYBR green master mix on the ViiA7 machine (Applied Biosystems, Waltham, MA). .. The expression of target genes was normalized to Gapdh.

    Microarray:

    Article Title: An eQTL Analysis of Partial Resistance to Puccinia hordei in Barley
    Article Snippet: Paragraph title: Microarray Processing ... Reverse transcription was performed using 5 µg of total RNA in a 45 µl reaction containing 50 ng/µl oligo d(T)18, 0.5 mM each dATP, dCTP, dGTP, 0.2 mM dTTP, 0.3 mM aa-dUTP, 10 mM DTT, and 400 U Superscript II (Invitrogen) in 1× reaction buffer.

    Incubation:

    Article Title: An eQTL Analysis of Partial Resistance to Puccinia hordei in Barley
    Article Snippet: Reverse transcription was performed using 5 µg of total RNA in a 45 µl reaction containing 50 ng/µl oligo d(T)18, 0.5 mM each dATP, dCTP, dGTP, 0.2 mM dTTP, 0.3 mM aa-dUTP, 10 mM DTT, and 400 U Superscript II (Invitrogen) in 1× reaction buffer. .. Primers and RNA were initially heated to 70°C for 10 min followed by cooling on ice, and the entire reaction incubated for 16 h at 42°C.

    Cell Culture:

    Article Title: Deficiency in the nuclear factor E2-related factor 2 renders pancreatic ?-cells vulnerable to arsenic-induced cell damage
    Article Snippet: Total RNA in cultured islets (500 islets each condition) was isolated using the RNeasy Mini Kit. .. Total RNA was reverse transcribed with MuLV reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA).

    Article Title: Degrading products of chondroitin sulfate can induce hypertrophy-like changes and MMP-13/ADAMTS5 production in chondrocytes
    Article Snippet: Real-time qPCR analysis Total RNA was isolated from cultured primary chondrocytes-laden 3D hydrogel using Easy Blue reagent (iNtRON biotechnology, Korea). .. The cDNA was synthesized from 2 μg of total RNA using reverse transcription kit (ELPIS Biotech, Korea) with random hexameter and oligo d(T) primer, and real-time qPCR was performed using the SYBR green master mix on the ViiA7 machine (Applied Biosystems, Waltham, MA).

    Expressing:

    Article Title: Degrading products of chondroitin sulfate can induce hypertrophy-like changes and MMP-13/ADAMTS5 production in chondrocytes
    Article Snippet: The cDNA was synthesized from 2 μg of total RNA using reverse transcription kit (ELPIS Biotech, Korea) with random hexameter and oligo d(T) primer, and real-time qPCR was performed using the SYBR green master mix on the ViiA7 machine (Applied Biosystems, Waltham, MA). .. The expression of target genes was normalized to Gapdh.

    Article Title: OsRDR6 plays role in host defense against double-stranded RNA virus, Rice Dwarf Phytoreovirus
    Article Snippet: Synthesize of cDNA was conducted using the Super Script III Reverse Transcriptase (Invitrogen) and an oligo d (T) primers instructed by the manufacture. .. Expression level of AtelF4A was used as an internal control.

    Modification:

    Article Title: Genetic transformation of Vitis vinifera via organogenesis
    Article Snippet: The system was slightly modified by the addition of Polyclar AT (95 mg g-1 of fresh tissue) and Na2 S2 O5 (0.4 %) to the homogenization buffer. .. Poly(A+ )RNA was isolated from total RNA using oligo d(T) Dynabeads (Dynal) following the manufacturer's protocol.

    Transformation Assay:

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ]. .. Bacteria were then transformed with the ligated nucleic acids, and plasmid DNA was prepared using commercially available kits (Qiaprep kit, Qiagen, Valencia, Calif., USA).

    Infection:

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data. .. For RT-PCR analysis of the TMV-U1 infection, primer SP6289 was used for the synthesis of first strand cDNA followed by PCR amplification of the movement protein region of TMV-U1.

    Light Microscopy:

    Article Title: Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
    Article Snippet: To determine parasite prevalence and load, midguts were dissected 8 days post-infection and parasite morphology and number were determined by fluorescent light microscopy (Zeiss, Jena, Germany). .. First strand cDNA syntheses were performed in a 20 μl reaction volume with 3 μg of total RNA, using oligo(dT) primers (Invitrogen, Carlsbad, CA, USA) and 200 U of Superscript Reverse Transcriptase II (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Expression of CRAMP via PGN-TLR-2 and of ?-defensin-3 via CpG-ODN-TLR-9 in corneal fibroblasts
    Article Snippet: For the reverse transcriptase (RT) reaction, total RNA (3 μg) with 0.5 µg oligo‐(dT)15‐18 (Invitrogen) was denatured at 70°C for 10 minutes. .. The polymerase chain reaction (PCR) reactions were performed with 1 µl of cDNA, 1X buffer, 1 mM MgCl2 , 200 µM of each dNTP, and 0.2 µM of each antimicrobial peptide, TLRs, and β actin specific primers (table 1 ).

    Article Title: Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
    Article Snippet: First strand cDNA syntheses were performed in a 20 μl reaction volume with 3 μg of total RNA, using oligo(dT) primers (Invitrogen, Carlsbad, CA, USA) and 200 U of Superscript Reverse Transcriptase II (Invitrogen). .. The quality of the Anopheles cDNAs was checked by PCR, using primers corresponding to the ribosomal protein S7, as described previously ( ).

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data. .. For RT-PCR analysis of the TMV-U1 infection, primer SP6289 was used for the synthesis of first strand cDNA followed by PCR amplification of the movement protein region of TMV-U1.

    Article Title: Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
    Article Snippet: Measurement of RNA Expression by qPCR To measure RNA transcription, the total unamplified RNA was DNase I treated (Ambion, Austin, USA) and reverse transcribed using oligo-d(T), dNTP, and M-MLV Reverse Transcriptase (all Invitrogen, Glostrup, Denmark). .. The cDNA was subjected to qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA, USA).

    Article Title: OsRDR6 plays role in host defense against double-stranded RNA virus, Rice Dwarf Phytoreovirus
    Article Snippet: Synthesize of cDNA was conducted using the Super Script III Reverse Transcriptase (Invitrogen) and an oligo d (T) primers instructed by the manufacture. .. The resulting cDNA was diluted 20 fold with water and subjected to 30 cycles of PCR reaction with gene-specific primers ( ).

    Sequencing:

    Article Title: Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
    Article Snippet: First strand cDNA syntheses were performed in a 20 μl reaction volume with 3 μg of total RNA, using oligo(dT) primers (Invitrogen, Carlsbad, CA, USA) and 200 U of Superscript Reverse Transcriptase II (Invitrogen). .. Amplifications were performed with SYBR Green PCR mastermix and analysed using the ABI PRISM 7700 sequence detection system following the manufacturer's instructions (Applied Biosystems, Foster City, CA, USA).

    Recombinant:

    Article Title: Mice Lacking Dystrophin or ? Sarcoglycan Spontaneously Develop Embryonal Rhabdomyosarcoma with Cancer-Associated p53 Mutations and Alternatively Spliced or Mutant Mdm2 Transcripts
    Article Snippet: A high-capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA) was used to reverse-transcribe 0.6 μg total RNA using Oligo d(T)16 primers (N8080128, Applied Biosystems, Foster City, CA), for endpoint reverse transcription (RT)-PCR, and random primers for quantitative (q)RT-PCR, in a 20 μl-reaction following the manufacturer's instructions. .. PCRs were performed using recombinant TaqDNA polymerase (10342-020, Invitrogen, Carlsbad, CA) following the manufacturer's instructions.

    Cellular Antioxidant Activity Assay:

    Article Title: PhotoMorphs(TM): A Novel Light-Activated Reagent for Controlling Gene Expression in Zebrafish
    Article Snippet: Reverse transcription reactions were performed using oligo(dT)20 primers and SuperScript™ II, according to the manufacturer’s protocol (Invitrogen). .. Primer sequences were: rheb -f: GGGG ACA AGT TTG TAC AAA AAA GCA GGC TCC ATG CCG CAG CCG AAA TCG C, rheb -rev: GGGG AC CAC TTT GTA CAA GAA AGC TGG GTC CAT CAT GGA GCA GGG CGT C, ef1 α-f: CTTCTCAGGCTGACTGTGC, ef1 α-rev: CCGCTAGCATTACCCTCC.

    Isolation:

    Article Title: Expression of CRAMP via PGN-TLR-2 and of ?-defensin-3 via CpG-ODN-TLR-9 in corneal fibroblasts
    Article Snippet: Paragraph title: RNA isolation and RT‐PCR analysis ... For the reverse transcriptase (RT) reaction, total RNA (3 μg) with 0.5 µg oligo‐(dT)15‐18 (Invitrogen) was denatured at 70°C for 10 minutes.

    Article Title: Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
    Article Snippet: Total RNA was isolated using TRIzol reagent (Invitrogen) and treated with deoxyribonuclease I. .. First strand cDNA syntheses were performed in a 20 μl reaction volume with 3 μg of total RNA, using oligo(dT) primers (Invitrogen, Carlsbad, CA, USA) and 200 U of Superscript Reverse Transcriptase II (Invitrogen).

    Article Title: Deficiency in the nuclear factor E2-related factor 2 renders pancreatic ?-cells vulnerable to arsenic-induced cell damage
    Article Snippet: Total RNA in cultured islets (500 islets each condition) was isolated using the RNeasy Mini Kit. .. Total RNA was reverse transcribed with MuLV reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA).

    Article Title: Degrading products of chondroitin sulfate can induce hypertrophy-like changes and MMP-13/ADAMTS5 production in chondrocytes
    Article Snippet: Real-time qPCR analysis Total RNA was isolated from cultured primary chondrocytes-laden 3D hydrogel using Easy Blue reagent (iNtRON biotechnology, Korea). .. The cDNA was synthesized from 2 μg of total RNA using reverse transcription kit (ELPIS Biotech, Korea) with random hexameter and oligo d(T) primer, and real-time qPCR was performed using the SYBR green master mix on the ViiA7 machine (Applied Biosystems, Waltham, MA).

    Article Title: Genetic transformation of Vitis vinifera via organogenesis
    Article Snippet: .. Poly(A+ )RNA was isolated from total RNA using oligo d(T) Dynabeads (Dynal) following the manufacturer's protocol. ..

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: Total RNA was isolated from the microdissected tissues using standard laboratory methods (TRIzol Reagent; Invitrogen, Carlsbad, Calif., USA). .. After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ].

    Size-exclusion Chromatography:

    Article Title: Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
    Article Snippet: Measurement of RNA Expression by qPCR To measure RNA transcription, the total unamplified RNA was DNase I treated (Ambion, Austin, USA) and reverse transcribed using oligo-d(T), dNTP, and M-MLV Reverse Transcriptase (all Invitrogen, Glostrup, Denmark). .. The program used was 95°C in 5 min followed by 45 cycles with 95°C in 10 sec., 62°C in 25 sec., and during the last cycle, a melting curve was made.

    Labeling:

    Article Title: An eQTL Analysis of Partial Resistance to Puccinia hordei in Barley
    Article Snippet: Microarray Processing Total RNA was labeled by indirect incorporation of fluorescent dyes f ollowing cDNA synthesis. .. Reverse transcription was performed using 5 µg of total RNA in a 45 µl reaction containing 50 ng/µl oligo d(T)18, 0.5 mM each dATP, dCTP, dGTP, 0.2 mM dTTP, 0.3 mM aa-dUTP, 10 mM DTT, and 400 U Superscript II (Invitrogen) in 1× reaction buffer.

    Mouse Assay:

    Article Title: Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
    Article Snippet: The P. berghei GFP-CON strain ( ) was passaged through CD1 or Balb/C mice and infections were performed using standard procedures ( ). .. First strand cDNA syntheses were performed in a 20 μl reaction volume with 3 μg of total RNA, using oligo(dT) primers (Invitrogen, Carlsbad, CA, USA) and 200 U of Superscript Reverse Transcriptase II (Invitrogen).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Expression of CRAMP via PGN-TLR-2 and of ?-defensin-3 via CpG-ODN-TLR-9 in corneal fibroblasts
    Article Snippet: Paragraph title: RNA isolation and RT‐PCR analysis ... For the reverse transcriptase (RT) reaction, total RNA (3 μg) with 0.5 µg oligo‐(dT)15‐18 (Invitrogen) was denatured at 70°C for 10 minutes.

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: Paragraph title: RT-qPCR and RT-PCR ... First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data.

    Article Title: Mice Lacking Dystrophin or ? Sarcoglycan Spontaneously Develop Embryonal Rhabdomyosarcoma with Cancer-Associated p53 Mutations and Alternatively Spliced or Mutant Mdm2 Transcripts
    Article Snippet: .. A high-capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA) was used to reverse-transcribe 0.6 μg total RNA using Oligo d(T)16 primers (N8080128, Applied Biosystems, Foster City, CA), for endpoint reverse transcription (RT)-PCR, and random primers for quantitative (q)RT-PCR, in a 20 μl-reaction following the manufacturer's instructions. .. For endpoint RT-PCR, 1 μl of each sample was used to amplify Pax3/7, Fkhr, p53, Mdm2, and βActin cDNAs separately.

    Article Title: Genetic transformation of Vitis vinifera via organogenesis
    Article Snippet: Paragraph title: RT-PCR analysis ... Poly(A+ )RNA was isolated from total RNA using oligo d(T) Dynabeads (Dynal) following the manufacturer's protocol.

    Article Title: PhotoMorphs(TM): A Novel Light-Activated Reagent for Controlling Gene Expression in Zebrafish
    Article Snippet: Paragraph title: RNA collection and RT-PCR ... Reverse transcription reactions were performed using oligo(dT)20 primers and SuperScript™ II, according to the manufacturer’s protocol (Invitrogen).

    Article Title: OsRDR6 plays role in host defense against double-stranded RNA virus, Rice Dwarf Phytoreovirus
    Article Snippet: Paragraph title: RT–PCR ... Synthesize of cDNA was conducted using the Super Script III Reverse Transcriptase (Invitrogen) and an oligo d (T) primers instructed by the manufacture.

    RNA Expression:

    Article Title: Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
    Article Snippet: .. Measurement of RNA Expression by qPCR To measure RNA transcription, the total unamplified RNA was DNase I treated (Ambion, Austin, USA) and reverse transcribed using oligo-d(T), dNTP, and M-MLV Reverse Transcriptase (all Invitrogen, Glostrup, Denmark). .. The cDNA was subjected to qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA, USA).

    cDNA Library Assay:

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: Paragraph title: cDNA Library Construction ... After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ].

    Chloramphenicol Acetyltransferase Assay:

    Article Title: PhotoMorphs(TM): A Novel Light-Activated Reagent for Controlling Gene Expression in Zebrafish
    Article Snippet: Reverse transcription reactions were performed using oligo(dT)20 primers and SuperScript™ II, according to the manufacturer’s protocol (Invitrogen). .. Primer sequences were: rheb -f: GGGG ACA AGT TTG TAC AAA AAA GCA GGC TCC ATG CCG CAG CCG AAA TCG C, rheb -rev: GGGG AC CAC TTT GTA CAA GAA AGC TGG GTC CAT CAT GGA GCA GGG CGT C, ef1 α-f: CTTCTCAGGCTGACTGTGC, ef1 α-rev: CCGCTAGCATTACCCTCC.

    Purification:

    Article Title: Mice Lacking Dystrophin or ? Sarcoglycan Spontaneously Develop Embryonal Rhabdomyosarcoma with Cancer-Associated p53 Mutations and Alternatively Spliced or Mutant Mdm2 Transcripts
    Article Snippet: RNA from mdx RMS or Sgca −/− RMS or from normal wild-type and mdx skeletal muscles (gastrocnemius or quadriceps) was extracted using the Trizol reagent (15596-026, Invitrogen, Carlsbad, CA) according to the manufacturer's instructions and further purified on a silica gel-based membrane (RNeasy Mini Kit, 74104, Qiagen, Valencia, CA). .. A high-capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA) was used to reverse-transcribe 0.6 μg total RNA using Oligo d(T)16 primers (N8080128, Applied Biosystems, Foster City, CA), for endpoint reverse transcription (RT)-PCR, and random primers for quantitative (q)RT-PCR, in a 20 μl-reaction following the manufacturer's instructions.

    Article Title: An eQTL Analysis of Partial Resistance to Puccinia hordei in Barley
    Article Snippet: Reverse transcription was performed using 5 µg of total RNA in a 45 µl reaction containing 50 ng/µl oligo d(T)18, 0.5 mM each dATP, dCTP, dGTP, 0.2 mM dTTP, 0.3 mM aa-dUTP, 10 mM DTT, and 400 U Superscript II (Invitrogen) in 1× reaction buffer. .. Purification of cDNA was performed using MinElute columns as recommended (Qiagen), substituting phosphate wash buffer (4.75 mM K2 HPO4 , 0.25 mM KH2 PO4 , 84% EtOH) for PB and phosphate elution buffer (3.8 mM K2 HPO4 , 0.2 mM KH2 PO4 ) for EB.

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ]. .. Nonrecombinant molecules were eliminated from the library by running Not I linearized clones on an agarose gel, followed by purification with β-agarase and recircularization.

    Plasmid Preparation:

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ]. .. The cDNAs were then blunt-end ligated to EcoR I adapters, digested with Not I and directionally cloned into a phagemid vector pT7T3-Pac.

    Real-time Polymerase Chain Reaction:

    Article Title: Deficiency in the nuclear factor E2-related factor 2 renders pancreatic ?-cells vulnerable to arsenic-induced cell damage
    Article Snippet: Paragraph title: Reverse transcription quantitative real-time PCR (RT-qPCR) ... Total RNA was reverse transcribed with MuLV reverse transcriptase and Oligo d(T) primers (Applied Biosystems, Foster City, CA).

    Article Title: TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity
    Article Snippet: .. First strand cDNA was prepared from 1 µg total RNA using the oligo d(T)primer and SuperScript II reverse transcriptase (Invitrogen). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). eIF4A or PP2A was used as the internal control to normalize the data. ..

    Article Title: Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
    Article Snippet: .. Measurement of RNA Expression by qPCR To measure RNA transcription, the total unamplified RNA was DNase I treated (Ambion, Austin, USA) and reverse transcribed using oligo-d(T), dNTP, and M-MLV Reverse Transcriptase (all Invitrogen, Glostrup, Denmark). .. The cDNA was subjected to qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Valencia, CA, USA).

    Article Title: Degrading products of chondroitin sulfate can induce hypertrophy-like changes and MMP-13/ADAMTS5 production in chondrocytes
    Article Snippet: .. The cDNA was synthesized from 2 μg of total RNA using reverse transcription kit (ELPIS Biotech, Korea) with random hexameter and oligo d(T) primer, and real-time qPCR was performed using the SYBR green master mix on the ViiA7 machine (Applied Biosystems, Waltham, MA). .. The expression of target genes was normalized to Gapdh.

    RNA Extraction:

    Article Title: Expression of CRAMP via PGN-TLR-2 and of ?-defensin-3 via CpG-ODN-TLR-9 in corneal fibroblasts
    Article Snippet: Total RNA extraction was performed with Trizol reagent. .. For the reverse transcriptase (RT) reaction, total RNA (3 μg) with 0.5 µg oligo‐(dT)15‐18 (Invitrogen) was denatured at 70°C for 10 minutes.

    Selection:

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: .. After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ]. .. The cDNAs were then blunt-end ligated to EcoR I adapters, digested with Not I and directionally cloned into a phagemid vector pT7T3-Pac.

    Agarose Gel Electrophoresis:

    Article Title: In silico Analysis of 2085 Clones from a Normalized Rat Vestibular Periphery 3? cDNA Library
    Article Snippet: After DNase treatment to eliminate genomic DNA, oligo-(dT) selection (Invitrogen) was used to isolate poly(A)+ RNA, prior to first-strand synthesis utilizing a Not I-(dT)18 oligonucleotide, followed by second-strand synthesis as described by D'Alessio et al. [ ]. .. Nonrecombinant molecules were eliminated from the library by running Not I linearized clones on an agarose gel, followed by purification with β-agarase and recircularization.

    Electrophoresis:

    Article Title: Mice Lacking Dystrophin or ? Sarcoglycan Spontaneously Develop Embryonal Rhabdomyosarcoma with Cancer-Associated p53 Mutations and Alternatively Spliced or Mutant Mdm2 Transcripts
    Article Snippet: RNA integrity was determined by capillary electrophoresis using 6000 Nano LabChip kit on a Bioanalyzer 2100 (Agilent, Foster City, CA). .. A high-capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA) was used to reverse-transcribe 0.6 μg total RNA using Oligo d(T)16 primers (N8080128, Applied Biosystems, Foster City, CA), for endpoint reverse transcription (RT)-PCR, and random primers for quantitative (q)RT-PCR, in a 20 μl-reaction following the manufacturer's instructions.

    Homogenization:

    Article Title: Genetic transformation of Vitis vinifera via organogenesis
    Article Snippet: The system was slightly modified by the addition of Polyclar AT (95 mg g-1 of fresh tissue) and Na2 S2 O5 (0.4 %) to the homogenization buffer. .. Poly(A+ )RNA was isolated from total RNA using oligo d(T) Dynabeads (Dynal) following the manufacturer's protocol.

    Spectrophotometry:

    Article Title: Mice Lacking Dystrophin or ? Sarcoglycan Spontaneously Develop Embryonal Rhabdomyosarcoma with Cancer-Associated p53 Mutations and Alternatively Spliced or Mutant Mdm2 Transcripts
    Article Snippet: RNA content was measured using an ND-1000 spectrophotometer (Nanodrop, Wilmington, DE). .. A high-capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA) was used to reverse-transcribe 0.6 μg total RNA using Oligo d(T)16 primers (N8080128, Applied Biosystems, Foster City, CA), for endpoint reverse transcription (RT)-PCR, and random primers for quantitative (q)RT-PCR, in a 20 μl-reaction following the manufacturer's instructions.

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    Thermo Fisher poly ra oligo dt assays
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