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Roche poly dt oligonucleotide
Poly Dt Oligonucleotide, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly dt oligonucleotide/product/Roche
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
poly dt oligonucleotide - by Bioz Stars, 2020-04
86/100 stars

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Clone Assay:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: For generation of RCC cell line 26A stably expressing Mcl-1-specific shRNA, RNAi sequences targeting Mcl-1 (5'-ACGCGGUAAUCGGACUCAA-3') and Luciferase mRNA (5'-GUGCGCUGCUGGUGCCAAC-3') were cloned in the GFP-expressing lentiviral vector pLVTHM (Dr. Didier Trono, Lausanne). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Transfection:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: At 48 h post transfection with siRNA, cells were assayed for gene knockdown or were used for further experiments. .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

shRNA:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: For generation of RCC cell line 26A stably expressing Mcl-1-specific shRNA, RNAi sequences targeting Mcl-1 (5'-ACGCGGUAAUCGGACUCAA-3') and Luciferase mRNA (5'-GUGCGCUGCUGGUGCCAAC-3') were cloned in the GFP-expressing lentiviral vector pLVTHM (Dr. Didier Trono, Lausanne). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Stable Transfection:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: For generation of RCC cell line 26A stably expressing Mcl-1-specific shRNA, RNAi sequences targeting Mcl-1 (5'-ACGCGGUAAUCGGACUCAA-3') and Luciferase mRNA (5'-GUGCGCUGCUGGUGCCAAC-3') were cloned in the GFP-expressing lentiviral vector pLVTHM (Dr. Didier Trono, Lausanne). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Quantitative RT-PCR:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: Total RNA was extracted from RCC lines after siRNA knockdown using RNeasy mini kit (Qiagen, Hilden, Germany) and analysed by quantitative RT-PCR. .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Real-time Polymerase Chain Reaction:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol. .. Quantitative PCR was performed using the LightCycler TaqMan Master Kit together with the Universal ProbeLibrary system (Roche).

Polymerase Chain Reaction:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: Paragraph title: RNAi and quantitative reverse transcriptase PCR ... RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Luciferase:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: For generation of RCC cell line 26A stably expressing Mcl-1-specific shRNA, RNAi sequences targeting Mcl-1 (5'-ACGCGGUAAUCGGACUCAA-3') and Luciferase mRNA (5'-GUGCGCUGCUGGUGCCAAC-3') were cloned in the GFP-expressing lentiviral vector pLVTHM (Dr. Didier Trono, Lausanne). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Expressing:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: For generation of RCC cell line 26A stably expressing Mcl-1-specific shRNA, RNAi sequences targeting Mcl-1 (5'-ACGCGGUAAUCGGACUCAA-3') and Luciferase mRNA (5'-GUGCGCUGCUGGUGCCAAC-3') were cloned in the GFP-expressing lentiviral vector pLVTHM (Dr. Didier Trono, Lausanne). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Sequencing:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: RNAi and quantitative reverse transcriptase PCR Cells from RCC lines were transfected with 20 nM of siRNA (depicted is the 19 nt portion in the sense strand of the targeted mRNA) specific for human Mcl-1 (5'-GGUUUGGCAUAUCUAAUAA-3'), Bfl-1/A1 (441 5'-GGAAGAAUUGUAACCAU-3' and 511 5'-CGGAUGUGGAUACCUAUAA-3'), Bim (5'-GGAAGAAUUGUAACCAUAU-3' and #2 5'-CCACUAUCUCAGUGCAAUG-30), Puma (5'-CCGAGAUGGAGCCCAAUUA-3' and #2 5'-CCUGGAGGGUCCUGUACAA-3'), Noxa (5'-AGUCGAGUGUGCUACUCAA-3') or p53 (5'-GUACCACCAUCCACUACAA-3') and a control siRNA (5'-GCGCAUUCCAGCUUACGUA-3'), containing a random sequence that does not match a sequence within the human or murine genome, using Lipofectamine RNAiMAX reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

Plasmid Preparation:

Article Title: Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1
Article Snippet: For generation of RCC cell line 26A stably expressing Mcl-1-specific shRNA, RNAi sequences targeting Mcl-1 (5'-ACGCGGUAAUCGGACUCAA-3') and Luciferase mRNA (5'-GUGCGCUGCUGGUGCCAAC-3') were cloned in the GFP-expressing lentiviral vector pLVTHM (Dr. Didier Trono, Lausanne). .. RNA (1 μg) was reverse transcribed using Expand Reverse Transcriptase and poly (dT) oligonucleotide (Roche) according to the manufacturer's protocol.

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    Roche neurons oligonucleotide probes
    Neurons Oligonucleotide Probes, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neurons oligonucleotide probes/product/Roche
    Average 86 stars, based on 1 article reviews
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    Roche poly dt oligonucleotide
    Poly Dt Oligonucleotide, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly dt oligonucleotide/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poly dt oligonucleotide - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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    Roche poly t oligo primers
    Poly T Oligo Primers, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly t oligo primers/product/Roche
    Average 92 stars, based on 2 article reviews
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    92/100 stars
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