Structured Review

Promega poly dt oligonucleotide
Poly Dt Oligonucleotide, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly dt oligonucleotide/product/Promega
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
poly dt oligonucleotide - by Bioz Stars, 2020-04
85/100 stars

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Isolation:

Article Title: Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1
Article Snippet: .. RNA was isolated from FACS® -sorted CD123high cells using TRIzol reagent (GIBCO BRL) according to the manufacturer's instructions and reverse transcribed using a poly-dT oligonucleotide (Promega) and 400 U Moloney MuLV reverse transcriptase (GIBCO BRL) at 42°C for 1 h. PCR assays were carried out in 50 μl reaction volumes using 1 μl cDNA template, 2 mM MgCl2 , 0.25 mM of each dNTP, 1 μM of each primer, and 3 U Taq polymerase (GIBCO BRL) in 1× buffer (10 mM Tris-HCl, pH 8.5, 50 mM KCl). ..

Polymerase Chain Reaction:

Article Title: Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1
Article Snippet: .. RNA was isolated from FACS® -sorted CD123high cells using TRIzol reagent (GIBCO BRL) according to the manufacturer's instructions and reverse transcribed using a poly-dT oligonucleotide (Promega) and 400 U Moloney MuLV reverse transcriptase (GIBCO BRL) at 42°C for 1 h. PCR assays were carried out in 50 μl reaction volumes using 1 μl cDNA template, 2 mM MgCl2 , 0.25 mM of each dNTP, 1 μM of each primer, and 3 U Taq polymerase (GIBCO BRL) in 1× buffer (10 mM Tris-HCl, pH 8.5, 50 mM KCl). ..

FACS:

Article Title: Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1
Article Snippet: .. RNA was isolated from FACS® -sorted CD123high cells using TRIzol reagent (GIBCO BRL) according to the manufacturer's instructions and reverse transcribed using a poly-dT oligonucleotide (Promega) and 400 U Moloney MuLV reverse transcriptase (GIBCO BRL) at 42°C for 1 h. PCR assays were carried out in 50 μl reaction volumes using 1 μl cDNA template, 2 mM MgCl2 , 0.25 mM of each dNTP, 1 μM of each primer, and 3 U Taq polymerase (GIBCO BRL) in 1× buffer (10 mM Tris-HCl, pH 8.5, 50 mM KCl). ..

Sequencing:

Article Title: Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1
Article Snippet: Paragraph title: Reverse Transcription PCR Assays and Sequencing of the PCR Product. ... RNA was isolated from FACS® -sorted CD123high cells using TRIzol reagent (GIBCO BRL) according to the manufacturer's instructions and reverse transcribed using a poly-dT oligonucleotide (Promega) and 400 U Moloney MuLV reverse transcriptase (GIBCO BRL) at 42°C for 1 h. PCR assays were carried out in 50 μl reaction volumes using 1 μl cDNA template, 2 mM MgCl2 , 0.25 mM of each dNTP, 1 μM of each primer, and 3 U Taq polymerase (GIBCO BRL) in 1× buffer (10 mM Tris-HCl, pH 8.5, 50 mM KCl).

Staining:

Article Title: Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1
Article Snippet: RNA was isolated from FACS® -sorted CD123high cells using TRIzol reagent (GIBCO BRL) according to the manufacturer's instructions and reverse transcribed using a poly-dT oligonucleotide (Promega) and 400 U Moloney MuLV reverse transcriptase (GIBCO BRL) at 42°C for 1 h. PCR assays were carried out in 50 μl reaction volumes using 1 μl cDNA template, 2 mM MgCl2 , 0.25 mM of each dNTP, 1 μM of each primer, and 3 U Taq polymerase (GIBCO BRL) in 1× buffer (10 mM Tris-HCl, pH 8.5, 50 mM KCl). .. The PCR products were separated on 1.2% agarose gels, stained with ethidium bromide, and analyzed by videodensitrometry using the Eagle Eye still video system and Eagle Sight Software (Stratagene).

Software:

Article Title: Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1
Article Snippet: RNA was isolated from FACS® -sorted CD123high cells using TRIzol reagent (GIBCO BRL) according to the manufacturer's instructions and reverse transcribed using a poly-dT oligonucleotide (Promega) and 400 U Moloney MuLV reverse transcriptase (GIBCO BRL) at 42°C for 1 h. PCR assays were carried out in 50 μl reaction volumes using 1 μl cDNA template, 2 mM MgCl2 , 0.25 mM of each dNTP, 1 μM of each primer, and 3 U Taq polymerase (GIBCO BRL) in 1× buffer (10 mM Tris-HCl, pH 8.5, 50 mM KCl). .. The PCR products were separated on 1.2% agarose gels, stained with ethidium bromide, and analyzed by videodensitrometry using the Eagle Eye still video system and Eagle Sight Software (Stratagene).

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  • 86
    Promega poly d
    Poly D, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly d/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poly d - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    85
    Promega poly dt oligonucleotide
    Poly Dt Oligonucleotide, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly dt oligonucleotide/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poly dt oligonucleotide - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

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