poly d lysine coated 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher poly d lysine coated 96 well plates
    Poly D Lysine Coated 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly d lysine coated 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    poly d lysine coated 96 well plates - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: The coding sequence of SR1 was amplified from genomic DNA of C57BL/6 mice and subcloned into the pcDNA3.1/TOPO vector (Invitrogen) with an N-terminal tag of the first 20 aa of rhodopsin. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Stable Transfection:

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: .. Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 . .. The following day, cells were transfected using Lipofectamine 2000 (Invitrogen).

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 . .. The following day, cells were transfected using Lipofectamine 2000 (Invitrogen).

    Construct:

    Article Title: The N-terminal region of RTP1S plays important roles in dimer formation and odorant receptor-trafficking
    Article Snippet: HEK293T cells were plated on poly-d -lysine–coated 96-well plates (Thermo Fisher Scientific). .. In addition, two luciferase constructs were used, including a firefly luciferase gene driven by a cAMP-response element (CRE-Luc) and a Renilla luciferase gene driven by a constitutively active SV40 promoter (pRL-SV40) that was used as an internal control for cell viability and transfection efficiency.

    Incubation:

    Article Title: Human Neuroglobin Functions as an Oxidative Stress-responsive Sensor for Neuroprotection *
    Article Snippet: Differentiated SH-SY5Y cells were plated on poly- d -lysine coated 96-well plates at a density of 5.0 × 105 cells/ml for 24 h. The pcDNA3.1-HNgb expression vector or control vector (pcDNA3.1 empty vector) was transfected by using LipofectamineTM 2000 (Invitrogen) according to the manufacturer's instructions. .. After 24 h of transfection, cells were treated with Sp-cAMPS or Rp-cAMPS at a concentration of 300 μ m for 1 h. The hydrogen peroxide was then added at 100 μ m , and cells were incubated for 24 h.

    Article Title: Functional Selectivity of CB2
    Article Snippet: Briefly, CHO K1 CNR2 or CHO K1 CNR1 cells were seeded at a density of 20,000 cells/well in poly-D-lysine–coated 96-well plates (Costar 3596, Thermo Fisher Scientific). .. On the following day, medium was replaced by 90 µ l of HBS/BSA (BSA: 0.2 mg/ml) and cells were challenged by adding 10 µ l of compound/drug (10X) followed by incubation for 90 minutes at 37°C. (Compounds were dissolved in dimethylsulfoxide or ethanol as 10 mM stock solutions and were formulated in HBS/BSA) After the initial 90-minute drug incubation, 25 µ l of PathHunter detection reagent was added to each well and the plate was further incubated for 60 minutes at room temperature.

    Article Title: Anti-Hepatitis C Virus Activity and Toxicity of Type III Phosphatidylinositol-4-Kinase Beta Inhibitors
    Article Snippet: INS-1E cells (passage 71) were plated in poly- d -lysine-coated 96-well plates at 50,000 cells per well in RPMI medium (Invitrogen) containing 11 mM glucose and 5% FBS. .. After overnight incubation, the GSIS assay was performed.

    Article Title: Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization
    Article Snippet: Cells were seeded at 105 cells per 100 μL loading medium (RPMI 1640, 10% FBS) into poly-d-lysine coated 96-well plates (Nalgene Nunc). .. Cells were incubated for 1 hour at 37°C before adding chemokine, and then the calcium flux peak was measured using a FlexStation 3 (Molecular Devices).

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: .. Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 . .. The following day, cells were transfected using Lipofectamine 2000 (Invitrogen).

    Article Title: The prostaglandin EP1 receptor potentiates kainate receptor activation via a protein kinase C pathway and exacerbates status epilepticus
    Article Snippet: Briefly, after dissociation the cells intended for neuron dense cultures were counted and plated onto poly-D-lysine coated 96-well plates (black with clear bottom) at a density of 150,000 cells/well for rat cultures and 100,000 cells/well for mouse cultures in Neurobasal medium supplemented with B27 and 5% FBS (Invitrogen, Carlsbad, CA). .. For astrocyte dense cultures isolated embryonic rat pup cortices were dissociated without enzyme digestion and were initially incubated in T-75 flasks in MEM supplemented with 10% FBS for 10 DIV.

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 . .. The following day, cells were transfected using Lipofectamine 2000 (Invitrogen).

    Luciferase:

    Article Title: The N-terminal region of RTP1S plays important roles in dimer formation and odorant receptor-trafficking
    Article Snippet: Paragraph title: Luciferase assay and data analysis ... HEK293T cells were plated on poly-d -lysine–coated 96-well plates (Thermo Fisher Scientific).

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: Paragraph title: Luciferase Assay in Hana3A Cells. ... Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 .

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: Renilla luciferase, driven by a constitutively active SV40 promoter (pRL-SV40; Promega), functioned as an internal control for transfection efficiency and cell viability. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Activity Assay:

    Article Title: Anti-Hepatitis C Virus Activity and Toxicity of Type III Phosphatidylinositol-4-Kinase Beta Inhibitors
    Article Snippet: A selective PI4KIIIβ inhibitor and a structurally similar compound lacking PI4KIIIβ and replicon activity were tested at 1 and 10 μM in a glucose-stimulated insulin secretion (GSIS) assay. .. INS-1E cells (passage 71) were plated in poly- d -lysine-coated 96-well plates at 50,000 cells per well in RPMI medium (Invitrogen) containing 11 mM glucose and 5% FBS.

    Expressing:

    Article Title: Human Neuroglobin Functions as an Oxidative Stress-responsive Sensor for Neuroprotection *
    Article Snippet: .. Differentiated SH-SY5Y cells were plated on poly- d -lysine coated 96-well plates at a density of 5.0 × 105 cells/ml for 24 h. The pcDNA3.1-HNgb expression vector or control vector (pcDNA3.1 empty vector) was transfected by using LipofectamineTM 2000 (Invitrogen) according to the manufacturer's instructions. .. After 24 h of transfection, cells were treated with Sp-cAMPS or Rp-cAMPS at a concentration of 300 μ m for 1 h. The hydrogen peroxide was then added at 100 μ m , and cells were incubated for 24 h.

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: .. Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 . .. The following day, cells were transfected using Lipofectamine 2000 (Invitrogen).

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 . .. The following day, cells were transfected using Lipofectamine 2000 (Invitrogen).

    Modification:

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: HEK293T cells were grown in Dulbecco’s modified Eagle medium containing 4.5 mg/ml glucose, 10% fetal bovine serum, and 1% penicillin/streptomycin. .. Cells were seeded in 50 μg/ml poly- d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology).

    Article Title: Nanosize Titanium Dioxide Stimulates Reactive Oxygen Species in Brain Microglia and Damages Neurons in Vitro
    Article Snippet: Cell culture Immortalized mouse BV2 microglia and rat N27 mesencephalic neurons were grown, respectively, in Dulbecco’s modified Eagle’s medium (DMEM) or RPMI 1640 medium, supplemented with 10% fetal calf serum (FCS) and 1% penicillin–streptomycin (ATCC, Manassas, VA). .. Tissue plugs of embryonic rat (Sprague-Dawley) brain striatum were purchased (BrainBits LLC, Springfield, IL; http://www.brainbitsllc.com ) and upon receipt were triturated and plated on poly-D -lysine-coated 96-well plates (Nalge Nunc International, Rochester, NY) in Neurobasal/ B27 media (Invitrogen, Carlsbad, CA).

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: FLIPR and compound screening HEK293T cells were grown in Dulbecco’s modified Eagle medium containing 4.5 mg/ml glucose, 10% fetal bovine serum, and 1% penicillin/streptomycin. .. Cells were seeded in 50 μg/ml poly-d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology).

    Derivative Assay:

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly- d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. For the initial screening, we screened about 3000 compounds derived from either the chemical library collected in Dr. Yu Rao’s lab, which is composed of different classes of heterocycles, including various drug scaffold or the Maybridge Ro3 Diversity Fragment Library purchased from Maybridge (Cambridge, UK), which consists of 2500 fragment compounds with a molecular weight below 350 and high rule of three compliance.

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly-d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. For the initial screening, we screened about 3000 compounds derived from either the chemical library collected in Dr. Yu Rao’s lab, which is composed of different classes of heterocycles, including various drug scaffold or the Maybridge Ro3 Diversity Fragment Library purchased from Maybridge (Cambridge, UK), which consists of 2500 fragment compounds with a molecular weight below 350 and high rule of three compliance.

    Transfection:

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly- d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. Two days after transfection the cells were washed with buffer containing 1× Hanks’ balanced salt solution (HBSS; 1.3 mM Ca2+ ) and 10 mM HEPES (pH 7.2).

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly-d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. Two days after transfection the cells were washed with buffer containing 1× Hanks’ balanced salt solution (HBSS; 1.3 mM Ca2+ ) and 10 mM HEPES (pH 7.2).

    Article Title: Human Neuroglobin Functions as an Oxidative Stress-responsive Sensor for Neuroprotection *
    Article Snippet: .. Differentiated SH-SY5Y cells were plated on poly- d -lysine coated 96-well plates at a density of 5.0 × 105 cells/ml for 24 h. The pcDNA3.1-HNgb expression vector or control vector (pcDNA3.1 empty vector) was transfected by using LipofectamineTM 2000 (Invitrogen) according to the manufacturer's instructions. .. After 24 h of transfection, cells were treated with Sp-cAMPS or Rp-cAMPS at a concentration of 300 μ m for 1 h. The hydrogen peroxide was then added at 100 μ m , and cells were incubated for 24 h.

    Article Title: The N-terminal region of RTP1S plays important roles in dimer formation and odorant receptor-trafficking
    Article Snippet: HEK293T cells were plated on poly-d -lysine–coated 96-well plates (Thermo Fisher Scientific). .. Plasmid DNAs of ORs and RTP1S were transfected using Lipofectamine 2000 (Invitrogen).

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: Renilla luciferase, driven by a constitutively active SV40 promoter (pRLSV40; Promega), functioned as an internal control for transfection efficiency and cell viability. .. Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 .

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: Renilla luciferase, driven by a constitutively active SV40 promoter (pRL-SV40; Promega), functioned as an internal control for transfection efficiency and cell viability. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Activation Assay:

    Article Title: Functional Selectivity of CB2
    Article Snippet: Upon cannabinoid receptor activation, recruitment of arrestin leads to formation of an active β galactosidase enzyme, which then acts on the substrate to emit light that can be detected by luminescence. .. Briefly, CHO K1 CNR2 or CHO K1 CNR1 cells were seeded at a density of 20,000 cells/well in poly-D-lysine–coated 96-well plates (Costar 3596, Thermo Fisher Scientific).

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: Firefly luciferase, driven by a cAMP response element promoter (CRE-Luc; Stratagene), was used to determine OR activation levels. .. Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 .

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: Firefly luciferase, driven by a cAMP response element promoter (CRE-Luc; Stratagene), was used to determine OR activation levels. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Cell Culture:

    Article Title: Nanosize Titanium Dioxide Stimulates Reactive Oxygen Species in Brain Microglia and Damages Neurons in Vitro
    Article Snippet: Paragraph title: Cell culture ... Tissue plugs of embryonic rat (Sprague-Dawley) brain striatum were purchased (BrainBits LLC, Springfield, IL; http://www.brainbitsllc.com ) and upon receipt were triturated and plated on poly-D -lysine-coated 96-well plates (Nalge Nunc International, Rochester, NY) in Neurobasal/ B27 media (Invitrogen, Carlsbad, CA).

    Article Title: PAN-811 Blocks Chemotherapy Drug-Induced In Vitro Neurotoxicity, While Not Affecting Suppression of Cancer Cell Growth
    Article Snippet: .. Neuronal Cell Culture Mixed cortical and striatal neurons from embryonic day 17 male Sprague-Dawley rats (tissue obtained from NIH) were seeded into poly-D-lysine coated 96-well plates at density of 50,000 cells/well and initially cultured at 37°C, 5% CO2 , in neurobasal medium (NB) with B27 supplement (Invitrogen) containing full strength of AOs to obtain highly enriched (95%) neurons [ ]. .. Since AOs, including vitamin E, vitamin E acetate, superoxide dismutase (SOD), catalase (CAT), and GSH, are additives to culture medium, reduction of AO concentration in culture medium provides an approach to determine the level of OS involvement in a neurotoxic process.

    Article Title: The prostaglandin EP1 receptor potentiates kainate receptor activation via a protein kinase C pathway and exacerbates status epilepticus
    Article Snippet: Paragraph title: Cortical cell culture ... Briefly, after dissociation the cells intended for neuron dense cultures were counted and plated onto poly-D-lysine coated 96-well plates (black with clear bottom) at a density of 150,000 cells/well for rat cultures and 100,000 cells/well for mouse cultures in Neurobasal medium supplemented with B27 and 5% FBS (Invitrogen, Carlsbad, CA).

    Sequencing:

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: The SR1 amino acid sequence is identical between 129 and C56BL/6 mice. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Molecular Weight:

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly- d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. For the initial screening, we screened about 3000 compounds derived from either the chemical library collected in Dr. Yu Rao’s lab, which is composed of different classes of heterocycles, including various drug scaffold or the Maybridge Ro3 Diversity Fragment Library purchased from Maybridge (Cambridge, UK), which consists of 2500 fragment compounds with a molecular weight below 350 and high rule of three compliance.

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly-d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. For the initial screening, we screened about 3000 compounds derived from either the chemical library collected in Dr. Yu Rao’s lab, which is composed of different classes of heterocycles, including various drug scaffold or the Maybridge Ro3 Diversity Fragment Library purchased from Maybridge (Cambridge, UK), which consists of 2500 fragment compounds with a molecular weight below 350 and high rule of three compliance.

    Fluorescence:

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly- d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. The 96-well plates were then transferred to the FLIPR Tetra (Molecular Device) for monitoring the GCAMP6s fluorescence before and after addition of the compounds that were prepared in separate compound plates.

    Article Title: A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel
    Article Snippet: Cells were seeded in 50 μg/ml poly-d -lysine-coated 96-well plates (3 × 104 cells/well) and allowed to grow for about 18 h, and then co-transfected with a total of 250 ng cDNA containing Piezo1 or Piezo2 and GCaMP6s using Lipofectamine 2000 (Invitrogen, Life Technology). .. The 96-well plates were then transferred to the FLIPR Tetra (Molecular Device) for monitoring the GCAMP6s fluorescence before and after addition of the compounds that were prepared in separate compound plates.

    Mutagenesis:

    Article Title: Functional Selectivity of CB2
    Article Snippet: These cells are engineered wherein an N-terminal deletion mutant of β -galactosidase enzyme (enzyme acceptor fragment) is fused with arrestin while a complimentary smaller fragment (ProLink) is fused with the C-terminal domain of a mouse cannabinoid receptor. .. Briefly, CHO K1 CNR2 or CHO K1 CNR1 cells were seeded at a density of 20,000 cells/well in poly-D-lysine–coated 96-well plates (Costar 3596, Thermo Fisher Scientific).

    Isolation:

    Article Title: The prostaglandin EP1 receptor potentiates kainate receptor activation via a protein kinase C pathway and exacerbates status epilepticus
    Article Snippet: The entire cortex was isolated from embryonic-day 16 to 18 embryos of either timed-pregnant Sprague-Dawley rats or wildtype C57BL/6 or EP1-KO mice. .. Briefly, after dissociation the cells intended for neuron dense cultures were counted and plated onto poly-D-lysine coated 96-well plates (black with clear bottom) at a density of 150,000 cells/well for rat cultures and 100,000 cells/well for mouse cultures in Neurobasal medium supplemented with B27 and 5% FBS (Invitrogen, Carlsbad, CA).

    Labeling:

    Article Title: Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization
    Article Snippet: Cells were seeded at 105 cells per 100 μL loading medium (RPMI 1640, 10% FBS) into poly-d-lysine coated 96-well plates (Nalgene Nunc). .. Data are shown as fluorescent counts and the y-axis labeled as Lm1.

    Mouse Assay:

    Article Title: The prostaglandin EP1 receptor potentiates kainate receptor activation via a protein kinase C pathway and exacerbates status epilepticus
    Article Snippet: The entire cortex was isolated from embryonic-day 16 to 18 embryos of either timed-pregnant Sprague-Dawley rats or wildtype C57BL/6 or EP1-KO mice. .. Briefly, after dissociation the cells intended for neuron dense cultures were counted and plated onto poly-D-lysine coated 96-well plates (black with clear bottom) at a density of 150,000 cells/well for rat cultures and 100,000 cells/well for mouse cultures in Neurobasal medium supplemented with B27 and 5% FBS (Invitrogen, Carlsbad, CA).

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: The SR1 amino acid sequence is identical between 129 and C56BL/6 mice. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Chloramphenicol Acetyltransferase Assay:

    Article Title: PAN-811 Blocks Chemotherapy Drug-Induced In Vitro Neurotoxicity, While Not Affecting Suppression of Cancer Cell Growth
    Article Snippet: Neuronal Cell Culture Mixed cortical and striatal neurons from embryonic day 17 male Sprague-Dawley rats (tissue obtained from NIH) were seeded into poly-D-lysine coated 96-well plates at density of 50,000 cells/well and initially cultured at 37°C, 5% CO2 , in neurobasal medium (NB) with B27 supplement (Invitrogen) containing full strength of AOs to obtain highly enriched (95%) neurons [ ]. .. Since AOs, including vitamin E, vitamin E acetate, superoxide dismutase (SOD), catalase (CAT), and GSH, are additives to culture medium, reduction of AO concentration in culture medium provides an approach to determine the level of OS involvement in a neurotoxic process.

    Immunocytochemistry:

    Article Title: The prostaglandin EP1 receptor potentiates kainate receptor activation via a protein kinase C pathway and exacerbates status epilepticus
    Article Snippet: Briefly, after dissociation the cells intended for neuron dense cultures were counted and plated onto poly-D-lysine coated 96-well plates (black with clear bottom) at a density of 150,000 cells/well for rat cultures and 100,000 cells/well for mouse cultures in Neurobasal medium supplemented with B27 and 5% FBS (Invitrogen, Carlsbad, CA). .. Cells from the neuron dense cultures were also plated onto poly-D-lysine coated coverslips in 24-well tissue culture plates in Neurobasal medium supplemented with B27 and 5% FBS for immunocytochemistry experiments at 7 DIV.

    Plasmid Preparation:

    Article Title: Human Neuroglobin Functions as an Oxidative Stress-responsive Sensor for Neuroprotection *
    Article Snippet: .. Differentiated SH-SY5Y cells were plated on poly- d -lysine coated 96-well plates at a density of 5.0 × 105 cells/ml for 24 h. The pcDNA3.1-HNgb expression vector or control vector (pcDNA3.1 empty vector) was transfected by using LipofectamineTM 2000 (Invitrogen) according to the manufacturer's instructions. .. After 24 h of transfection, cells were treated with Sp-cAMPS or Rp-cAMPS at a concentration of 300 μ m for 1 h. The hydrogen peroxide was then added at 100 μ m , and cells were incubated for 24 h.

    Article Title: The N-terminal region of RTP1S plays important roles in dimer formation and odorant receptor-trafficking
    Article Snippet: HEK293T cells were plated on poly-d -lysine–coated 96-well plates (Thermo Fisher Scientific). .. Plasmid DNAs of ORs and RTP1S were transfected using Lipofectamine 2000 (Invitrogen).

    Article Title: Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism
    Article Snippet: Hana3A cells, a HEK293T-derived cell line stably expressing the receptor-transporting proteins (RTP1L and RTP2), receptor expression-enhancing protein 1 (REEP1), and olfactory G protein (Gαolf ) , were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37 °C and 5% CO2 . .. For each 96-well plate, 0.5 μg of pRL-SV40, 1 μg of CRE-Luc, 0.5 μg of mouse RTP1S, 0.25 μg of mouse muscarinic acetylcholine receptor M3 and 0.5 μg of receptor plasmid DNA were transfected.

    Article Title: SR1, a Mouse Odorant Receptor with an Unusually Broad Response Profile
    Article Snippet: The coding sequence of SR1 was amplified from genomic DNA of C57BL/6 mice and subcloned into the pcDNA3.1/TOPO vector (Invitrogen) with an N-terminal tag of the first 20 aa of rhodopsin. .. Hana3A cells stably expressing RTP1L, RTP2, REEP1, and Gα olf were plated on poly- d -lysine-coated 96-well plates (Nalge Nunc) and incubated overnight in minimum essential medium eagle (Sigma) with 10% FBS at 37°C and 5% CO2 .

    Concentration Assay:

    Article Title: PAN-811 Blocks Chemotherapy Drug-Induced In Vitro Neurotoxicity, While Not Affecting Suppression of Cancer Cell Growth
    Article Snippet: Neuronal Cell Culture Mixed cortical and striatal neurons from embryonic day 17 male Sprague-Dawley rats (tissue obtained from NIH) were seeded into poly-D-lysine coated 96-well plates at density of 50,000 cells/well and initially cultured at 37°C, 5% CO2 , in neurobasal medium (NB) with B27 supplement (Invitrogen) containing full strength of AOs to obtain highly enriched (95%) neurons [ ]. .. Since AOs, including vitamin E, vitamin E acetate, superoxide dismutase (SOD), catalase (CAT), and GSH, are additives to culture medium, reduction of AO concentration in culture medium provides an approach to determine the level of OS involvement in a neurotoxic process.

    Article Title: Human Neuroglobin Functions as an Oxidative Stress-responsive Sensor for Neuroprotection *
    Article Snippet: Differentiated SH-SY5Y cells were plated on poly- d -lysine coated 96-well plates at a density of 5.0 × 105 cells/ml for 24 h. The pcDNA3.1-HNgb expression vector or control vector (pcDNA3.1 empty vector) was transfected by using LipofectamineTM 2000 (Invitrogen) according to the manufacturer's instructions. .. After 24 h of transfection, cells were treated with Sp-cAMPS or Rp-cAMPS at a concentration of 300 μ m for 1 h. The hydrogen peroxide was then added at 100 μ m , and cells were incubated for 24 h.

    Transmigration Assay:

    Article Title: Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization
    Article Snippet: Paragraph title: Changes in [Ca2+ ]i, chemotaxis, and transmigration ... Cells were seeded at 105 cells per 100 μL loading medium (RPMI 1640, 10% FBS) into poly-d-lysine coated 96-well plates (Nalgene Nunc).

    Chemotaxis Assay:

    Article Title: Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization
    Article Snippet: Paragraph title: Changes in [Ca2+ ]i, chemotaxis, and transmigration ... Cells were seeded at 105 cells per 100 μL loading medium (RPMI 1640, 10% FBS) into poly-d-lysine coated 96-well plates (Nalgene Nunc).

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    Thermo Fisher poly d lysine coated 96 well plates
    Poly D Lysine Coated 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly d lysine coated 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    poly d lysine coated 96 well plates - by Bioz Stars, 2020-04
    93/100 stars
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