poly a mrna pools  (Millipore)


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    Name:
    Poly A RNA
    Description:
    Sigma offers a broad range of high quality mRNA isolated from various tissue and cell sources Each mRNA is purified using a combination of either oligo dT cellulose chromatography or proprietary isolation methods Following purification the integrity of the mRNA is assessed using denatured agarose gel or formaldehyde gel electrophoresis followed by Northern blot analysis Non human mRNA is prepared from Sprague Dawley rats and from BALB c mice
    Catalog Number:
    p0230
    Price:
    None
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    Structured Review

    Millipore poly a mrna pools
    Sigma offers a broad range of high quality mRNA isolated from various tissue and cell sources Each mRNA is purified using a combination of either oligo dT cellulose chromatography or proprietary isolation methods Following purification the integrity of the mRNA is assessed using denatured agarose gel or formaldehyde gel electrophoresis followed by Northern blot analysis Non human mRNA is prepared from Sprague Dawley rats and from BALB c mice
    https://www.bioz.com/result/poly a mrna pools/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poly a mrna pools - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Down-Regulation of 5-HT1B and 5-HT1D Receptors Inhibits Proliferation, Clonogenicity and Invasion of Human Pancreatic Cancer Cells
    Article Snippet: .. Plated cells were transfected with double-stranded siRNA targeting the mRNA of the serotonergic receptors (5-HT-1) subtype –B or –D (Sigma-Aldrich, St. Louis, MO), or transfected with control (non-silencing) siRNA; (5′-AAUUCUCCGAACGUGUCACGU-3′) , (Sigma-Aldrich, St. Louis, MO). siRNA targeting tissue transglutaminase (TG2) (Qiagen, Valencia, CA) were also employed . .. Cells were transfected with either siRNA, at a final concentration of 25–50 nM for 72 h, using HiPerFect Transfection Reagent (Qiagen, Valencia, CA) according to the manufacturer's protocol.

    Amplification:

    Article Title: Feline Immunodeficiency Virus Envelope Glycoproteins Antagonize Tetherin through a Distinctive Mechanism That Requires Virion Incorporation
    Article Snippet: .. A table that details all oligonucleotides used in this work is available on request. cDNAs corresponding to fcTetherinLF , accession no. , and fcTetherinSF , accession no. DDBJ , were amplified by reverse transcriptase PCR (RT-PCR) from mRNA of feline PBMCs pretreated with 1,000 U/ml rat IFN-α (Sigma). .. A dog tetherin cDNA was isolated by RT-PCR using mRNA of D17 cells, also pretreated with 1,000 U/ml rat IFN-α.

    Stable Transfection:

    Article Title: Destabilization of Interleukin-6 mRNA Requires a Putative RNA Stem-Loop Structure, an AU-Rich Element, and the RNA-Binding Protein AUF1 ▿
    Article Snippet: .. To perform IP of mRNA with AUF1, 0.5 × 106 to 1 × 106 NIH 3T3 cells stably expressing myc-tagged AUF1 isoforms and GFP mRNA constructs were lysed in 400 μl CelLytic-M cell lysis reagent (C-2978; Sigma). .. Of the first 200 μl (before IP), one-eighth (25 μl) was used for Western blot analysis of myc-AUF1 and the rest for RNA preparation (RNA-total).

    Polymerase Chain Reaction:

    Article Title: Activation of interferon regulatory factor-3 via toll-like receptor 3 and immunomodulatory functions detected in A549 lung epithelial cells exposed to misplaced U1-snRNA
    Article Snippet: .. Detection of mRNA by standard polymerase chain reaction (RT-PCR) analysis Total RNA was isolated using TRI-Reagent (Sigma-Aldrich) according to the manufacturer's instructions. .. As IFN-β mRNA only consists of one single exon, it was impossible to design exon-spanning primers that exclude amplification of potentially contaminating genomic DNA.

    Article Title: Feline Immunodeficiency Virus Envelope Glycoproteins Antagonize Tetherin through a Distinctive Mechanism That Requires Virion Incorporation
    Article Snippet: .. A table that details all oligonucleotides used in this work is available on request. cDNAs corresponding to fcTetherinLF , accession no. , and fcTetherinSF , accession no. DDBJ , were amplified by reverse transcriptase PCR (RT-PCR) from mRNA of feline PBMCs pretreated with 1,000 U/ml rat IFN-α (Sigma). .. A dog tetherin cDNA was isolated by RT-PCR using mRNA of D17 cells, also pretreated with 1,000 U/ml rat IFN-α.

    Isolation:

    Article Title: Activation of interferon regulatory factor-3 via toll-like receptor 3 and immunomodulatory functions detected in A549 lung epithelial cells exposed to misplaced U1-snRNA
    Article Snippet: .. Detection of mRNA by standard polymerase chain reaction (RT-PCR) analysis Total RNA was isolated using TRI-Reagent (Sigma-Aldrich) according to the manufacturer's instructions. .. As IFN-β mRNA only consists of one single exon, it was impossible to design exon-spanning primers that exclude amplification of potentially contaminating genomic DNA.

    Article Title: Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication
    Article Snippet: .. Generation of lentiviral vectors carrying an IFN cDNA library A Gateway-compatible cDNA library was generated from mRNA isolated from HeLa cells that had been treated with 1,000 U/ml type I IFN (a mixture of human interferon α and ω, Sigma) for 24 h. Briefly, total RNA was extracted using the RNeasy Mini Kit (Qiagen), and mRNA was then isolated using a PolyATtract mRNA Isolation System II (Promega) according to the manufacturer’s recommendations. .. The cDNA was synthesized using the CloneMiner cDNA Library Construction Kit (Life Technologies) from 3 μg of mRNA and fractionated with cDNA Size Fractionation Columns (Life Technologies).

    Construct:

    Article Title: Destabilization of Interleukin-6 mRNA Requires a Putative RNA Stem-Loop Structure, an AU-Rich Element, and the RNA-Binding Protein AUF1 ▿
    Article Snippet: .. To perform IP of mRNA with AUF1, 0.5 × 106 to 1 × 106 NIH 3T3 cells stably expressing myc-tagged AUF1 isoforms and GFP mRNA constructs were lysed in 400 μl CelLytic-M cell lysis reagent (C-2978; Sigma). .. Of the first 200 μl (before IP), one-eighth (25 μl) was used for Western blot analysis of myc-AUF1 and the rest for RNA preparation (RNA-total).

    Purification:

    Article Title: A novel type II membrane receptor up‐regulated by IFN‐α in fibroblasts functions in cell proliferation through the JAK‐STAT signalling pathway
    Article Snippet: .. mRNA was harvested from 5 × 106 KMB‐17 cells treated with IFN‐α (Sigma, St Louis, MO, USA) at a concentration of 100 µ/ml for 6 h at 37 °C by the QuickPrep micro mRNA purification kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA). ..

    Concentration Assay:

    Article Title: A novel type II membrane receptor up‐regulated by IFN‐α in fibroblasts functions in cell proliferation through the JAK‐STAT signalling pathway
    Article Snippet: .. mRNA was harvested from 5 × 106 KMB‐17 cells treated with IFN‐α (Sigma, St Louis, MO, USA) at a concentration of 100 µ/ml for 6 h at 37 °C by the QuickPrep micro mRNA purification kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA). ..

    Generated:

    Article Title: Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication
    Article Snippet: .. Generation of lentiviral vectors carrying an IFN cDNA library A Gateway-compatible cDNA library was generated from mRNA isolated from HeLa cells that had been treated with 1,000 U/ml type I IFN (a mixture of human interferon α and ω, Sigma) for 24 h. Briefly, total RNA was extracted using the RNeasy Mini Kit (Qiagen), and mRNA was then isolated using a PolyATtract mRNA Isolation System II (Promega) according to the manufacturer’s recommendations. .. The cDNA was synthesized using the CloneMiner cDNA Library Construction Kit (Life Technologies) from 3 μg of mRNA and fractionated with cDNA Size Fractionation Columns (Life Technologies).

    cDNA Library Assay:

    Article Title: Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication
    Article Snippet: .. Generation of lentiviral vectors carrying an IFN cDNA library A Gateway-compatible cDNA library was generated from mRNA isolated from HeLa cells that had been treated with 1,000 U/ml type I IFN (a mixture of human interferon α and ω, Sigma) for 24 h. Briefly, total RNA was extracted using the RNeasy Mini Kit (Qiagen), and mRNA was then isolated using a PolyATtract mRNA Isolation System II (Promega) according to the manufacturer’s recommendations. .. The cDNA was synthesized using the CloneMiner cDNA Library Construction Kit (Life Technologies) from 3 μg of mRNA and fractionated with cDNA Size Fractionation Columns (Life Technologies).

    Expressing:

    Article Title: Destabilization of Interleukin-6 mRNA Requires a Putative RNA Stem-Loop Structure, an AU-Rich Element, and the RNA-Binding Protein AUF1 ▿
    Article Snippet: .. To perform IP of mRNA with AUF1, 0.5 × 106 to 1 × 106 NIH 3T3 cells stably expressing myc-tagged AUF1 isoforms and GFP mRNA constructs were lysed in 400 μl CelLytic-M cell lysis reagent (C-2978; Sigma). .. Of the first 200 μl (before IP), one-eighth (25 μl) was used for Western blot analysis of myc-AUF1 and the rest for RNA preparation (RNA-total).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Activation of interferon regulatory factor-3 via toll-like receptor 3 and immunomodulatory functions detected in A549 lung epithelial cells exposed to misplaced U1-snRNA
    Article Snippet: .. Detection of mRNA by standard polymerase chain reaction (RT-PCR) analysis Total RNA was isolated using TRI-Reagent (Sigma-Aldrich) according to the manufacturer's instructions. .. As IFN-β mRNA only consists of one single exon, it was impossible to design exon-spanning primers that exclude amplification of potentially contaminating genomic DNA.

    Article Title: Feline Immunodeficiency Virus Envelope Glycoproteins Antagonize Tetherin through a Distinctive Mechanism That Requires Virion Incorporation
    Article Snippet: .. A table that details all oligonucleotides used in this work is available on request. cDNAs corresponding to fcTetherinLF , accession no. , and fcTetherinSF , accession no. DDBJ , were amplified by reverse transcriptase PCR (RT-PCR) from mRNA of feline PBMCs pretreated with 1,000 U/ml rat IFN-α (Sigma). .. A dog tetherin cDNA was isolated by RT-PCR using mRNA of D17 cells, also pretreated with 1,000 U/ml rat IFN-α.

    Lysis:

    Article Title: Destabilization of Interleukin-6 mRNA Requires a Putative RNA Stem-Loop Structure, an AU-Rich Element, and the RNA-Binding Protein AUF1 ▿
    Article Snippet: .. To perform IP of mRNA with AUF1, 0.5 × 106 to 1 × 106 NIH 3T3 cells stably expressing myc-tagged AUF1 isoforms and GFP mRNA constructs were lysed in 400 μl CelLytic-M cell lysis reagent (C-2978; Sigma). .. Of the first 200 μl (before IP), one-eighth (25 μl) was used for Western blot analysis of myc-AUF1 and the rest for RNA preparation (RNA-total).

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  • 99
    Millipore in vitro transcription
    In Vitro Transcription, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro transcription/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    in vitro transcription - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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