poietics peripheral blood mononuclear cells pbmcs  (Lonza)


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    Name:
    Human Peripheral Blood Mononuclear Cells
    Description:
    Mononuclear cells from human peripheral blood cryopreserved 10 million cells
    Catalog Number:
    4w-270
    Price:
    None
    Category:
    Primary and Stem Cells
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    Structured Review

    Lonza poietics peripheral blood mononuclear cells pbmcs
    Mononuclear cells from human peripheral blood cryopreserved 10 million cells
    https://www.bioz.com/result/poietics peripheral blood mononuclear cells pbmcs/product/Lonza
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poietics peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    In Vitro:

    Article Title: Kv1.3 Channel Blockade Modulates the Effector Function of B Cells in Granulomatosis with Polyangiitis
    Article Snippet: .. Briefly, peripheral blood mononuclear cells (PBMCs) were isolated from GPA patients, who produce ANCA upon in vitro induction , and stored in RPMI 1640 (Lonza, Basel, Switzerland) supplemented with 50 µg/mL gentamycin (GIBCO, Life Technologies, Grand Island, NY, USA), 10% fetal calf serum (FCS, Lonza), and 10% dimethyl sulfoxide (DMSO). ..

    Co-Culture Assay:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: .. Experiments were conducted in 48-well plates, where co-culture of 40,000 ECC1 cells/well and 8 × 105 female human PBMCs (Lonza, Australia) cells/well were seeded with RPMI-1640 medium (Sigma-Aldrich, Australia) containing 10% heat inactivated FCS (Life Technologies, Australia), 120 μg/ml streptomycin (Sigma-Aldrich, Australia), 50 μg/ml Gentamycin (Gibco, Australia), 37 °C, 5% CO2 . .. In addition, control plates with either ECC1 cells alone or human PBMCs alone were used.

    Isolation:

    Article Title: P2X7 Receptor Induces Tumor Necrosis Factor-α Converting Enzyme Activation and Release to Boost TNF-α Production
    Article Snippet: .. Human peripheral blood mononuclear cells were isolated following standard procedure ( ) and cultured for 16 h in RPMI 1640 medium (Lonza) with 10% of FCS, 2 mM Glutamax, and 100 U/ml penicillin–streptomycin (Life Technologies). .. After monocyte adherence, cells were washed and primed for 4 h with LPS (10 ng/ml), and then cells were washed or not with physiological buffer and incubated in the same buffer at 37°C with 3 mM of ATP for 20 min.

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    Article Title: Kv1.3 Channel Blockade Modulates the Effector Function of B Cells in Granulomatosis with Polyangiitis
    Article Snippet: .. Briefly, peripheral blood mononuclear cells (PBMCs) were isolated from GPA patients, who produce ANCA upon in vitro induction , and stored in RPMI 1640 (Lonza, Basel, Switzerland) supplemented with 50 µg/mL gentamycin (GIBCO, Life Technologies, Grand Island, NY, USA), 10% fetal calf serum (FCS, Lonza), and 10% dimethyl sulfoxide (DMSO). ..

    Cell Culture:

    Article Title: P2X7 Receptor Induces Tumor Necrosis Factor-α Converting Enzyme Activation and Release to Boost TNF-α Production
    Article Snippet: .. Human peripheral blood mononuclear cells were isolated following standard procedure ( ) and cultured for 16 h in RPMI 1640 medium (Lonza) with 10% of FCS, 2 mM Glutamax, and 100 U/ml penicillin–streptomycin (Life Technologies). .. After monocyte adherence, cells were washed and primed for 4 h with LPS (10 ng/ml), and then cells were washed or not with physiological buffer and incubated in the same buffer at 37°C with 3 mM of ATP for 20 min.

    Activation Assay:

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    other:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: In addition, control plates with either ECC1 cells alone or human PBMCs alone were used.

    Infection:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: .. In addition, infection of human PBMCs alone with live C. trachomatis , without the azithromycin treatment, resulted in significantly reduced, yet apparent infectivity (p < 0.0001). .. The number of chlamydial IFUs measured from ECC1 and PBMCs co-culture was significantly reduced in comparison to infected ECC1 culture alone (p < 0.0001) by almost two logs.

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  • 99
    Lonza poietics peripheral blood mononuclear cells pbmcs
    Poietics Peripheral Blood Mononuclear Cells Pbmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poietics peripheral blood mononuclear cells pbmcs/product/Lonza
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    poietics peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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