pnk mix  (New England Biolabs)


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  • 99
    Name:
    T4 Polynucleotide Kinase
    Description:
    T4 Polynucleotide Kinase 2 500 units
    Catalog Number:
    m0201l
    Price:
    228
    Size:
    2 500 units
    Category:
    Polynucleotide Kinases
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    Structured Review

    New England Biolabs pnk mix
    T4 Polynucleotide Kinase
    T4 Polynucleotide Kinase 2 500 units
    https://www.bioz.com/result/pnk mix/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pnk mix - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: tRNA-derived fragments and microRNAs in the maternal-fetal interface of a mouse maternal-immune-activation autism model
    Article Snippet: .. T4 PNK treatment and RT-PCR 1 μg total RNA was treated with T4 PNK (NEB) in the presence of 10 mM ATP at 37 °C for 40 minutes and then cleaned up by ethanol precipitation. ..

    Ethanol Precipitation:

    Article Title: tRNA-derived fragments and microRNAs in the maternal-fetal interface of a mouse maternal-immune-activation autism model
    Article Snippet: .. T4 PNK treatment and RT-PCR 1 μg total RNA was treated with T4 PNK (NEB) in the presence of 10 mM ATP at 37 °C for 40 minutes and then cleaned up by ethanol precipitation. ..

    Labeling:

    Article Title: Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen), and small RNA samples were 5′ labeled by first treating with calf alkaline phosphatase (Roche) followed by phosphorylation with T4 polynucleotide kinase (NEB) and 32 P-γ-ATP. .. Small RNA samples were 3′ labeled using T4 RNA ligase (NEB) and 32 P-pCp as described by the manufacturer.

    Purification:

    Article Title: CspA regulation of Staphylococcus aureus carotenoid levels and σB activity is controlled by YjbH and Spx.
    Article Snippet: .. To decipher interactions between proteins and the cspA promoter, a PCR product consisting of −300 to +1 of the cspA promoter was first end-labeled with γ−32P-ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) and purified by MicroSpin G-50 columns (GE). .. His6 -Spx and/or αCTD were pre-incubated for 10 min at 25oC in 18 μL of binding buffer (20 mM Tris-HCl pH 7.8, 50 mM NaCl, 5 mM MgCl2, 10% glycerol) containing 0.5 μg of calf thymus DNA.

    Produced:

    Article Title: PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2
    Article Snippet: .. DNA from the MNase concentration that produced the greatest SSB/DSB ratio (0.015 U) was mock-treated or treated with T4 PNK in the presence of 2 mM ATP and 10U T4 PNK enzyme (wild-type or 3′-phosphatase dead; New England Biolabs). .. The synthetic oligonucleotide sequences (MWG or Eurogentec) employed to generate duplex substrates are listed in .

    Concentration Assay:

    Article Title: PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2
    Article Snippet: .. DNA from the MNase concentration that produced the greatest SSB/DSB ratio (0.015 U) was mock-treated or treated with T4 PNK in the presence of 2 mM ATP and 10U T4 PNK enzyme (wild-type or 3′-phosphatase dead; New England Biolabs). .. The synthetic oligonucleotide sequences (MWG or Eurogentec) employed to generate duplex substrates are listed in .

    Incubation:

    Article Title: Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities
    Article Snippet: .. Samples were treated with CIP or T4 PNK by addition of “Cutsmart” or “PNK” buffer from New England Biolabs and 10 units of CIP or T4 PNK and incubation at 37 °C for 15 min. Mock treated samples contained only Cutsmart buffer and water in lieu of CIP or T4 PNK. ..

    other:

    Article Title: Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei
    Article Snippet: Small RNAs were size-selected ( ) and treated with a variety of enzymes under manufacturer-recommended conditions: Calf intestinal alkaline phosphatase (CIP; Amersham) and T4 polynucleotide kinase (T4 PNK; New England Biolabs) assays were carried out for 1 h at 37 °C, and Terminator exonuclease (Epicentre) was added for 1 h at 30 °C.

    Isolation:

    Article Title: Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen), and small RNA samples were 5′ labeled by first treating with calf alkaline phosphatase (Roche) followed by phosphorylation with T4 polynucleotide kinase (NEB) and 32 P-γ-ATP. .. Small RNA samples were 3′ labeled using T4 RNA ligase (NEB) and 32 P-pCp as described by the manufacturer.

    Polymerase Chain Reaction:

    Article Title: CspA regulation of Staphylococcus aureus carotenoid levels and σB activity is controlled by YjbH and Spx.
    Article Snippet: .. To decipher interactions between proteins and the cspA promoter, a PCR product consisting of −300 to +1 of the cspA promoter was first end-labeled with γ−32P-ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) and purified by MicroSpin G-50 columns (GE). .. His6 -Spx and/or αCTD were pre-incubated for 10 min at 25oC in 18 μL of binding buffer (20 mM Tris-HCl pH 7.8, 50 mM NaCl, 5 mM MgCl2, 10% glycerol) containing 0.5 μg of calf thymus DNA.

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  • 99
    New England Biolabs pnk reaction mix
    Pnk Reaction Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk reaction mix/product/New England Biolabs
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    pnk reaction mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs pnk mixture
    Pnk Mixture, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk mixture/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pnk mixture - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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