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Dunnett’s Comparison of <t>PNGase</t> <t>F</t> Between the Control Enzyme (New England Biolabs P0705L, E1) and Other Enzymes (E2–E7). Means for each enzyme were compared to the control (E1) using ANOVA with Dunnett’s test. Statistically significant differences from the control enzyme (E1, bold red) are shown in black (enzyme labels and circles). Red indicates no significant difference from the control. The circle size reflects the standard deviation of measurements. The y -axis represents the % Peak Area, which is the relative abundance of each peak in relation to the total integrated peaks from 20 to 65 min.
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Dunnett’s Comparison of PNGase F Between the Control Enzyme (New England Biolabs <t>P0705L,</t> E1) and Other Enzymes (E2–E7). Means for each enzyme were compared to the control (E1) using ANOVA with Dunnett’s test. Statistically significant differences from the control enzyme (E1, bold red) are shown in black (enzyme labels and circles). Red indicates no significant difference from the control. The circle size reflects the standard deviation of measurements. The y -axis represents the % Peak Area, which is the relative abundance of each peak in relation to the total integrated peaks from 20 to 65 min.
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Dunnett’s Comparison of PNGase F Between the Control Enzyme (New England Biolabs P0705L, E1) and Other Enzymes (E2–E7). Means for each enzyme were compared to the control (E1) using ANOVA with Dunnett’s test. Statistically significant differences from the control enzyme (E1, bold red) are shown in black (enzyme labels and circles). Red indicates no significant difference from the control. The circle size reflects the standard deviation of measurements. The y -axis represents the % Peak Area, which is the relative abundance of each peak in relation to the total integrated peaks from 20 to 65 min.

Journal: Antibodies

Article Title: Robustness Evaluation of a Legacy N-Glycan Profiling Method for a Therapeutic Antibody Under ICH Q14 Lifecycle Principles

doi: 10.3390/antib15010009

Figure Lengend Snippet: Dunnett’s Comparison of PNGase F Between the Control Enzyme (New England Biolabs P0705L, E1) and Other Enzymes (E2–E7). Means for each enzyme were compared to the control (E1) using ANOVA with Dunnett’s test. Statistically significant differences from the control enzyme (E1, bold red) are shown in black (enzyme labels and circles). Red indicates no significant difference from the control. The circle size reflects the standard deviation of measurements. The y -axis represents the % Peak Area, which is the relative abundance of each peak in relation to the total integrated peaks from 20 to 65 min.

Article Snippet: PNGase F enzymes from various brands were sourced from New England Biolabs (Ipswich, MA, USA for P0704L, P0705L, P0705S), Agilent Technologies (Santa Clara, CA, USA for AdvanceBio GKE-5005B), Promega (Madison, WI, USA for V4831), Thermo Fisher Scientific (Waltham, MA, USA for GIBCO A39245), and N-Zyme Scientifics (Doylestown, PA, USA for NZPP050 via Bulldog Bio Inc.).

Techniques: Comparison, Control, Standard Deviation

Dunnett’s Comparison of PNGase F Between the Control Enzyme (New England Biolabs P0705L, E1) and Other Enzymes (E2–E7). Means for each enzyme were compared to the control (E1) using ANOVA with Dunnett’s test. Statistically significant differences from the control enzyme (E1, bold red) are shown in black (enzyme labels and circles). Red indicates no significant difference from the control. The circle size reflects the standard deviation of measurements. The y -axis represents the % Peak Area, which is the relative abundance of each peak in relation to the total integrated peaks from 20 to 65 min.

Journal: Antibodies

Article Title: Robustness Evaluation of a Legacy N-Glycan Profiling Method for a Therapeutic Antibody Under ICH Q14 Lifecycle Principles

doi: 10.3390/antib15010009

Figure Lengend Snippet: Dunnett’s Comparison of PNGase F Between the Control Enzyme (New England Biolabs P0705L, E1) and Other Enzymes (E2–E7). Means for each enzyme were compared to the control (E1) using ANOVA with Dunnett’s test. Statistically significant differences from the control enzyme (E1, bold red) are shown in black (enzyme labels and circles). Red indicates no significant difference from the control. The circle size reflects the standard deviation of measurements. The y -axis represents the % Peak Area, which is the relative abundance of each peak in relation to the total integrated peaks from 20 to 65 min.

Article Snippet: Analysis of variance (ANOVA) was performed using JMP ® Pro (Version 17.2.0) to evaluate alternative enzymes and SPE cartridges in comparison with the control sources, specifically New England Biolabs’ P0705L enzyme and the OASIS SPE cartridge.

Techniques: Comparison, Control, Standard Deviation