pmsf  (Beyotime)

 
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    Name:
    PMSF
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    Catalog Number:
    ST505
    Price:
    130.0
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    Structured Review

    Beyotime pmsf

    https://www.bioz.com/result/pmsf/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-04
    97/100 stars

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    Related Articles

    Western Blot:

    Article Title: Long non-coding RNA Taurine upregulated gene 1 promotes osteosarcoma cell metastasis by mediating HIF-1α via miR-143-5p
    Article Snippet: .. Western blot Total protein of OS cells was extracted using RIPA lysis buffer (Beyotime, China) containing 1% PMSF (Beyotime, China). .. The primary antibodies used were human antibody TGF-β, HIF-1α, E-cadherin, N-cadherin, Vimentin, Slug, Twist, and GAPDH (Cell Signaling Technology, USA).

    Article Title: Downregulation of PTPRK Promotes Cell Proliferation and Metastasis of NSCLC by Enhancing STAT3 Activation
    Article Snippet: .. Western Blotting Analysis The cells were lysed and isolated in NETN lysis buffer (20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) supplemented with 1 mM PMSF (Beyotime, Haimen, China) [ ]. .. Protein concentration was assessed using a Quick Start Bradford protein assay kit (Bio-Rad).

    Lysis:

    Article Title: Long non-coding RNA Taurine upregulated gene 1 promotes osteosarcoma cell metastasis by mediating HIF-1α via miR-143-5p
    Article Snippet: .. Western blot Total protein of OS cells was extracted using RIPA lysis buffer (Beyotime, China) containing 1% PMSF (Beyotime, China). .. The primary antibodies used were human antibody TGF-β, HIF-1α, E-cadherin, N-cadherin, Vimentin, Slug, Twist, and GAPDH (Cell Signaling Technology, USA).

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells
    Article Snippet: .. After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China). ..

    Article Title: Dexmedetomidine Ameliorates Acute Stress-Induced Kidney Injury by Attenuating Oxidative Stress and Apoptosis through Inhibition of the ROS/JNK Signaling Pathway
    Article Snippet: Concentrations of norepinephrine (NE), epinephrine, and corticosterone (CORT) in the serum of each group of rats were determined by enzyme-linked immunosorbent assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions. .. Frozen kidney tissues were cut into small pieces, lysed with RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) supplemented with phenylmethanesulfonyl fluoride (PMSF) (Beyotime Biotechnology, Shanghai, China), homogenized with a Tissue Prep instrument (Bio-Xplorer International Limited, Beijing, China), and then centrifuged at 12000 ×g for 10 min at 4°C to collect supernatant. .. Nuclear and plasma proteins were extracted using a nuclear-cytosol extraction kit (Beyotime Biotechnology, Shanghai, China).

    Article Title: Downregulation of PTPRK Promotes Cell Proliferation and Metastasis of NSCLC by Enhancing STAT3 Activation
    Article Snippet: .. Western Blotting Analysis The cells were lysed and isolated in NETN lysis buffer (20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) supplemented with 1 mM PMSF (Beyotime, Haimen, China) [ ]. .. Protein concentration was assessed using a Quick Start Bradford protein assay kit (Bio-Rad).

    Protein Concentration:

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells
    Article Snippet: .. After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China). ..

    Article Title: HMGN5 promotes IL-6-induced epithelial-mesenchymal transition of bladder cancer by interacting with Hsp27
    Article Snippet: .. After lysing the target cells with RIPA buffer supplemented with 1% PMSF, we extracted the proteins and determined the protein concentration using the bicinchoninic acid (BCA) protein assay kit (Beyotime Institute of Biotechnology, China). .. An SDS-PAGE minigel was used for protein separation and the proteins were then transferred onto a PVDF membrane, which was then probed with the following antibodies (dilution 1:1000) : anti-Hsp27 (ab2790, Abcam), anti-p-Hsp27 (ab5594, Abcam), anti-HMGN5 (ab56031, Abcam), anti-E-cadherin (ab1416, Abcam), anti-Vimentin (ab8978, Abcam), anti-STAT3 (ab68153, Abcam), anti-p-STAT3 (ab76315, Tyr705, Abcam), and anti-GAPDH (ab8245, Abcam) at 4°C overnight followed by the incubation with the HRP-conjugated secondary antibody (dilution 1:5000).

    BIA-KA:

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells
    Article Snippet: .. After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China). ..

    Radio Immunoprecipitation:

    Article Title: The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection
    Article Snippet: For p65, the cytoplasmic and nuclear protein extractions were prepared with the nuclear and cytoplasm protein extraction kit (KGP1100, KeyGenBioTECH, Nanjing, China) following the manufacturer provided protocol. .. For iNOS, independent samples were extracted with a radio immunoprecipitation assay buffer (RIPA) supplemented with 1:100 PMSF (Beyotime, Beijing, China). ..

    Mouse Assay:

    Article Title: Recapitulating X-Linked Juvenile Retinoschisis in Mouse Model by Knock-In Patient-Specific Novel Mutation
    Article Snippet: Nuclei were stained with DAPI. .. Retinas from WT or RS1 -KI mice were lysed in RIPA buffer with PMSF, pH 7.4 (Beyotime, China). .. Total protein was determined based on the bicinchoninic acid (BCA) method using the BCA Protein Assay Kit (Beyotime, China).

    Isolation:

    Article Title: Downregulation of PTPRK Promotes Cell Proliferation and Metastasis of NSCLC by Enhancing STAT3 Activation
    Article Snippet: .. Western Blotting Analysis The cells were lysed and isolated in NETN lysis buffer (20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) supplemented with 1 mM PMSF (Beyotime, Haimen, China) [ ]. .. Protein concentration was assessed using a Quick Start Bradford protein assay kit (Bio-Rad).

    Bicinchoninic Acid Protein Assay:

    Article Title: HMGN5 promotes IL-6-induced epithelial-mesenchymal transition of bladder cancer by interacting with Hsp27
    Article Snippet: .. After lysing the target cells with RIPA buffer supplemented with 1% PMSF, we extracted the proteins and determined the protein concentration using the bicinchoninic acid (BCA) protein assay kit (Beyotime Institute of Biotechnology, China). .. An SDS-PAGE minigel was used for protein separation and the proteins were then transferred onto a PVDF membrane, which was then probed with the following antibodies (dilution 1:1000) : anti-Hsp27 (ab2790, Abcam), anti-p-Hsp27 (ab5594, Abcam), anti-HMGN5 (ab56031, Abcam), anti-E-cadherin (ab1416, Abcam), anti-Vimentin (ab8978, Abcam), anti-STAT3 (ab68153, Abcam), anti-p-STAT3 (ab76315, Tyr705, Abcam), and anti-GAPDH (ab8245, Abcam) at 4°C overnight followed by the incubation with the HRP-conjugated secondary antibody (dilution 1:5000).

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  • 97
    Beyotime pmsf
    Western blotting for inducible nitric oxide synthase (iNOS) in ED19 chicken embryo hearts and hatchling chicken hearts. Fertile chicken eggs were air cell injected with vehicle, PFOA 2 mg/kg, or PFOA 2 mg/kg + l -carnitine 100 mg/kg, and then incubated to ED19 or hatch. At the desired stage, hearts were collected, protein was extracted with <t>RIPA</t> buffer supplemented with <t>PMSF</t> and then assessed with western blotting, and the expressions were normalized to control animals. ( A ) iNOS relative expression levels of ED19 chicken embryo hearts; ( B ) iNOS relative expression levels of hatchling chicken embryo hearts. N = 3 per group. * Statistically different from control group ( p
    Pmsf, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    98
    Beyotime pmsf ripa
    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% <t>PMSF-RIPA</t> and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P
    Pmsf Ripa, supplied by Beyotime, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf ripa/product/Beyotime
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pmsf ripa - by Bioz Stars, 2021-04
    98/100 stars
      Buy from Supplier

    Image Search Results


    Western blotting for inducible nitric oxide synthase (iNOS) in ED19 chicken embryo hearts and hatchling chicken hearts. Fertile chicken eggs were air cell injected with vehicle, PFOA 2 mg/kg, or PFOA 2 mg/kg + l -carnitine 100 mg/kg, and then incubated to ED19 or hatch. At the desired stage, hearts were collected, protein was extracted with RIPA buffer supplemented with PMSF and then assessed with western blotting, and the expressions were normalized to control animals. ( A ) iNOS relative expression levels of ED19 chicken embryo hearts; ( B ) iNOS relative expression levels of hatchling chicken embryo hearts. N = 3 per group. * Statistically different from control group ( p

    Journal: International Journal of Molecular Sciences

    Article Title: The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection

    doi: 10.3390/ijms18061229

    Figure Lengend Snippet: Western blotting for inducible nitric oxide synthase (iNOS) in ED19 chicken embryo hearts and hatchling chicken hearts. Fertile chicken eggs were air cell injected with vehicle, PFOA 2 mg/kg, or PFOA 2 mg/kg + l -carnitine 100 mg/kg, and then incubated to ED19 or hatch. At the desired stage, hearts were collected, protein was extracted with RIPA buffer supplemented with PMSF and then assessed with western blotting, and the expressions were normalized to control animals. ( A ) iNOS relative expression levels of ED19 chicken embryo hearts; ( B ) iNOS relative expression levels of hatchling chicken embryo hearts. N = 3 per group. * Statistically different from control group ( p

    Article Snippet: For iNOS, independent samples were extracted with a radio immunoprecipitation assay buffer (RIPA) supplemented with 1:100 PMSF (Beyotime, Beijing, China).

    Techniques: Western Blot, Injection, Incubation, Expressing

    ALLM blocked the AR down-regulation in the presence of triptolide. ( A ) LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h, the AR protein level was analyzed by western blot assay; ( B ) The relative density of AR (normalized with GAPDH in western blot assay) after LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h; ( C ) The western bolt assay of AR protein levels in LNCaP cells after the cells were co-treated with triptolide (100 nM) and ALLM (50 μM); ( D ) The relative density of AR (normalized with triptolide) after the cells were co-treated with triptolide (100 nM) and ALLM (50 and 100 μM). (* p

    Journal: Medicina

    Article Title: Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells

    doi: 10.3390/medicina54030039

    Figure Lengend Snippet: ALLM blocked the AR down-regulation in the presence of triptolide. ( A ) LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h, the AR protein level was analyzed by western blot assay; ( B ) The relative density of AR (normalized with GAPDH in western blot assay) after LNCaP cells were co-treated with triptolide (100 nM) and ALLM (100 μM) or PMSF (100 μM) for 24 h; ( C ) The western bolt assay of AR protein levels in LNCaP cells after the cells were co-treated with triptolide (100 nM) and ALLM (50 μM); ( D ) The relative density of AR (normalized with triptolide) after the cells were co-treated with triptolide (100 nM) and ALLM (50 and 100 μM). (* p

    Article Snippet: After co-treatment with triptolide and ALLM (50 μM and 100 μM), PMSF (100 μM) or BAPTA-AM (10 μM) for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime Biotechnology, Haimen, Jiangsu, China) and the protein concentration was detected with BCA kit (Beyotime Biotechnology, Haimen, Jiangsu, China).

    Techniques: Western Blot

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    doi: 10.1155/2018/2305683

    Figure Lengend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Article Snippet: Western Blotting Assay Total protein product was extracted by incubating cells with 1% PMSF-RIPA (Catl. number ST506, Catl. number P0013B, Beyotime Biotechnology, China) and then centrifuged at 12000 rpm for 10 min. β -Actin was selected as the internal reference protein.

    Techniques: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot

    Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT

    doi: 10.1155/2018/2305683

    Figure Lengend Snippet: Detection of Notch Signaling . (a) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h and then subjected to RNA Purified Total RNA Extraction Kit to collect total RNA. The expression levels of Notch, Jagged, Hes1, and Hes5 were detected with RT 2 -PCR method. Administration of LBP up-regulated expression of Notch, Jagged, Hes1, and Hes5 at mRNA level. (b) 5 × 10 5 /ml DCs were incubated with LBP at 100 μ g/ml for 48 h. Then cells were incubated with 1% PMSF-RIPA and the centrifuged at 12000 rpm for 10 min to collect total protein. The expression levels of Notch and Jagged were detected with western blotting assay. Administration of LBP up-regulated expression of Notch and Jagged at protein. Each assay was represented by three independent replicates. ∗∗ P

    Article Snippet: Total protein product was extracted by incubating cells with 1% PMSF-RIPA (Catl. number ST506, Catl. number P0013B, Beyotime Biotechnology, China) and then centrifuged at 12000 rpm for 10 min. β -Actin was selected as the internal reference protein.

    Techniques: Incubation, Purification, RNA Extraction, Expressing, Polymerase Chain Reaction, Western Blot