pm 14 c l arginine monohydrochloride  (Millipore)


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    Structured Review

    Millipore pm 14 c l arginine monohydrochloride
    Pm 14 C L Arginine Monohydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pm 14 c l arginine monohydrochloride/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pm 14 c l arginine monohydrochloride - by Bioz Stars, 2020-03
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    Positive Control:

    Article Title: Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice. Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice
    Article Snippet: IL‐6 was infused through a sidearm syringe infusion pump into the arterial supply of the pancreas or the proximal small intestine to reach a final concentration of 100 ng/mL. l ‐Arginine monohydrochloride (10 mmol/L) (Sigma‐Aldrich, Steinheim, Germany, cat.no. .. A6969) dissolved in perfusion buffer was used as a positive control to the perfused pancreas.

    Synthesized:

    Article Title: Listeria monocytogenes aguA1, but Not aguA2, Encodes a Functional Agmatine Deiminase
    Article Snippet: Agmatine sulfate, l -arginine monohydrochloride, l -citrulline, diacetyl-monoxime, and thiosemicarbazid were purchased from Sigma-Aldrich. .. N -Carbamoyl-putrescine was synthesized by the Institute of Pesticide and Environmental Toxicology or Zhejiang University.

    Construct:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: The construct (pET-CK9) encodes an identical CK9 mature amino acid sequence with the addition of methionine at the N-terminus and a his-tag (GSGHHHHHHHH) added at the C-terminus for purification. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Incubation:

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: .. Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicilli n–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either light L-lysine monohydrochloride and L-arginine monohydrochloride (as above) or R10 L-arginine monohydrochloride (referred to as heavy arginine; CK Isotopes, cat. # CNLM-539) and K8 L-lysine monohydrochloride (referred to as heavy lysine; CK Isotopes, cat. # CNLM-291) for several passages ( > 15 cell divisions) before expansion in four 10-cm dishes per sample.

    Article Title: AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
    Article Snippet: .. Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in and samples were prepared as follows: Wildtype and FICD-/- CHO-K1 cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco; Life Technologies cat. # 26400-044), 1 x Penicillin-Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma cat. # P5607-25G), 62.5 mg/l “light” L-lysine monohydrochloride (Sigma cat. # L8662-25G) and 60.5 mg/l “light” L-arginine monohydrochloride (Sigma cat. # A6969-25G) and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either 60.5 mg/l “light” (as above) or “heavy” L-arginine monohydrochloride (R10; U-13C6, U-15N4; Cambridge Isotope Laboratories, Inc. cat. # CNLM-539, Andover, MO) for several passages ( > 15 cell divisions) before expansion in five 10 cm dishes per sample.

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: .. The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicillin–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either light L-lysine monohydrochloride and L-arginine monohydrochloride (as above) or R10 L-arginine monohydrochloride (re ferred to as heavy arginine; CK Isotopes, cat. # CNLM-539) and K8 L-lysine monohydrochloride (referred to as heavy lysine; CK Isotopes, cat. # CNLM-291) for several passages ( > 15 cell divisions) before expansion in four 10-cm dishes per sample.

    Activity Assay:

    Article Title: Tissue-type plasminogen activator exerts EGF-like chemokinetic effects on oligodendrocytes in white matter (re)myelination
    Article Snippet: The actilyse buffer was reconstituted with arginine monohydrochloride (Sigma-Aldrich-Aldrich) added to GGACK-tPA. .. Finally, the lack of proteolytic activity of GGACK-tPA was confirmed with a spectrozyme assay (American Diagnostica).

    Modification:

    Article Title: Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice. Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice
    Article Snippet: The perfusion medium consisted of a modified Krebs‐Ringer bicarbonate buffer containing in addition 5% dextran T‐70 (Pharmacosmos, Holbæk, Denmark), 0.1% bovine serum albumin (Faction V, Merck, Ballerup, Denmark), 3.5 mmol/L glucose, and 5 mmol/L pyruvate, fumarate and glutamate. .. IL‐6 was infused through a sidearm syringe infusion pump into the arterial supply of the pancreas or the proximal small intestine to reach a final concentration of 100 ng/mL. l ‐Arginine monohydrochloride (10 mmol/L) (Sigma‐Aldrich, Steinheim, Germany, cat.no.

    Article Title: Anti-Inflammatory Effects of 5α,8α-Epidioxycholest-6-en-3β-ol, a Steroidal Endoperoxide Isolated from Aplysia depilans, Based on Bioguided Fractionation and NMR Analysis
    Article Snippet: Reagents and Standards LPS from Escherichia coli , sodium pyruvate, sulfanilamide, MTT, Triton X-100, N -(naphth-1-yl)ethylenediamine dihydrochloride, β-nicotinamide adenine dinucleotide reduced form (NADH), sodium deoxycholate, dimethyl sulfoxide (DMSO), trizma hydrochloride, trypan blue, soybean lipoxygenase (LOX) from Glycine max (L.) Merr. (Type V-S; EC 1.13.11.12), phospholipase A2 (PLA2 ) from honey bee (Apis mellifera ) venom (EC.3.1.1.4), propan-2-ol, butan-1-ol, sodium nitroprusside (SNP), l -arginine monohydrochloride, diacetyl monoxime, antipyrine E, H2 SO4 , bovine serum albumin (BSA), chloroform, and N -methyl-l -arginine acetate salt were from Sigma-Aldrich (St. Louis, MO, USA). .. Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Hank’s balanced salt solution (HBSS) and Pen Strep solution (penicillin 5000 units/mL and streptomycin 5000 µg/mL) were purchased from GIBCO, Invitrogen (Grand Island, NE, USA). β-Tubulin primary antibody, as well as anti-rabbit secondary antibody, were from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: Phosphatase of Regenerating Liver 3 (PRL3) Provokes a Tyrosine Phosphoproteome to Drive Prometastatic Signal Transduction *
    Article Snippet: Dulbecco's Modification of Eagles Medium (DMEM) with 4.5g/L glucose, l -glutamine, sodium pyruvate, and penicillin-streptomycin (5000 I.U/ml penicillin; 5000 μ/ml streptomycin) were from Mediatech, Inc. (Manassas, VA). .. Dialyzed fetal bovine serum for SILAC, l -lysine monohydrochloride, l -arginine monohydrochloride, l -lysine 13 C hydrochloride, l -arginine 13 C hydrochloride, agarose-conjugated anti-phosphotyrosine monoclonal antibody PT66, dithiothreitol (DTT), iodoacetamide, and trypsin were from Sigma-Aldrich (St. Louis, MO).

    Article Title: Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site -specific histone methylation
    Article Snippet: 2 Culture medium: Minimum Essential Medium Eagle, Joklik modification (SAFC Biosciences). .. 3 Components of homemade medium (all from Sigma-Aldrich): L-Arginine monohydrochloride; L-Cysteine dihydrochloride; L-Histidine dihydrochloride; L-Isoleucine; L-Leucine; L-Lysine monohydrochloride; L-Phenylalanine; L-Threonine; L-Tryptophan; L-Tyrosine; L-Valine; Choline chloride; Folic acid; myo-Inositol; Niacinamide; D-Pantothenic acid hemicalcium salt; Pyridoxal hydrochloride; Riboflavin; Thiamine hydrochloride; MgCl2 ; KCl; NaCl; Na2 HPO4 ; Glucose; Phenol Red sodium salt; NaHCO3 .

    Transformation Assay:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Following transformation of the pET-CK9 plasmid into BL21 Star (DE3) competent cells (Invitrogen), the induction of recombinant protein production, purification under denaturing conditions, refolding, re-purification under native conditions, SDS-PAGE analysis of proteins and quantification of protein concentration were as described previously [ , , ]. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Cell Culture:

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: .. For Heavy methyl SILAC labeling, HeLa S3 cells were cultured in ‘Light’ and ‘Heavy’ SILAC media (PAA, custom) depleted of lysine, arginine and methionine and supplemented with l -arginine (Sigma Aldrich, A6969) l -lysine (Sigma Aldrich, L8662), and either l -[13 CD3]-methionine (Met-4, heavy, Sigma Aldrich, 299154) or l -[12 CH3]-methionine (Met-0, light, Sigma Aldrich M5308), as previously described ( ). .. For standard SILAC labeling, HeLa cells were grown in ‘‘Light’’ and ‘‘Heavy’’ SILAC DMEM (Thermo Fisher Scientific, 88420) supplemented with either L-arginine and L-lysine, or their heavy isotope-counterparts l -arginine-13 C6 , 15 N4 hydrochloride (Arg10, Sigma, 608033) and L-lysine-13 C6 , 15 N2 hydrochloride (Lys 8, Sigma, 608041) ( ).

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: .. Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicilli n–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either light L-lysine monohydrochloride and L-arginine monohydrochloride (as above) or R10 L-arginine monohydrochloride (referred to as heavy arginine; CK Isotopes, cat. # CNLM-539) and K8 L-lysine monohydrochloride (referred to as heavy lysine; CK Isotopes, cat. # CNLM-291) for several passages ( > 15 cell divisions) before expansion in four 10-cm dishes per sample.

    Article Title: Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site -specific histone methylation
    Article Snippet: Paragraph title: 2.1. Mammalian cell culture and heavy methionine labeling ... 3 Components of homemade medium (all from Sigma-Aldrich): L-Arginine monohydrochloride; L-Cysteine dihydrochloride; L-Histidine dihydrochloride; L-Isoleucine; L-Leucine; L-Lysine monohydrochloride; L-Phenylalanine; L-Threonine; L-Tryptophan; L-Tyrosine; L-Valine; Choline chloride; Folic acid; myo-Inositol; Niacinamide; D-Pantothenic acid hemicalcium salt; Pyridoxal hydrochloride; Riboflavin; Thiamine hydrochloride; MgCl2 ; KCl; NaCl; Na2 HPO4 ; Glucose; Phenol Red sodium salt; NaHCO3 .

    Article Title: AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
    Article Snippet: .. Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in and samples were prepared as follows: Wildtype and FICD-/- CHO-K1 cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco; Life Technologies cat. # 26400-044), 1 x Penicillin-Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma cat. # P5607-25G), 62.5 mg/l “light” L-lysine monohydrochloride (Sigma cat. # L8662-25G) and 60.5 mg/l “light” L-arginine monohydrochloride (Sigma cat. # A6969-25G) and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either 60.5 mg/l “light” (as above) or “heavy” L-arginine monohydrochloride (R10; U-13C6, U-15N4; Cambridge Isotope Laboratories, Inc. cat. # CNLM-539, Andover, MO) for several passages ( > 15 cell divisions) before expansion in five 10 cm dishes per sample.

    Article Title: Arginine reprogramming in ADPKD results in arginine-dependent cystogenesis
    Article Snippet: Paragraph title: Cell culture. ... Arginine-defined media were MEK medium prepared with arginine, lysine, and glutamine-free DMEM/F-12 (Caisson Laboratories), supplemented with 0.5 mM lysine (Sigma-Aldrich), 2.5 mM GlutaMAX (ThermoFisher), and various concentrations of l -arginine monohydrochloride (Sigma-Aldrich).

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: .. The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicillin–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either light L-lysine monohydrochloride and L-arginine monohydrochloride (as above) or R10 L-arginine monohydrochloride (re ferred to as heavy arginine; CK Isotopes, cat. # CNLM-539) and K8 L-lysine monohydrochloride (referred to as heavy lysine; CK Isotopes, cat. # CNLM-291) for several passages ( > 15 cell divisions) before expansion in four 10-cm dishes per sample.

    Generated:

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: HeLa Flp-In T-REx cells were in-house generated according to the guidelines of Thermo Fisher Scientific and grown in DMEM supplemented with 10% FBS Tetracycline free (Euroclone, ECS01822), 1% glutamine and 100 U/ml Penicillin and Streptomycin. .. For Heavy methyl SILAC labeling, HeLa S3 cells were cultured in ‘Light’ and ‘Heavy’ SILAC media (PAA, custom) depleted of lysine, arginine and methionine and supplemented with l -arginine (Sigma Aldrich, A6969) l -lysine (Sigma Aldrich, L8662), and either l -[13 CD3]-methionine (Met-4, heavy, Sigma Aldrich, 299154) or l -[12 CH3]-methionine (Met-0, light, Sigma Aldrich M5308), as previously described ( ).

    other:

    Article Title: A Method for Sporulating Budding Yeast Cells That Allows for Unbiased Identification of Kinase Substrates Using Stable Isotope Labeling by Amino Acids in Cell Culture
    Article Snippet: To make a 250× solution of light lysine and arginine (3% lysine, 2% arginine), 3 g L-lysine-HCl (Sigma #L5626) and 2 g l -arginine-HCl (Sigma #A5131) are dissolved in 100 ml water and the solution is filter-sterilized.

    Protein Concentration:

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicilli n–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. After normalization of the post-nuclear supernatants for equal total protein concentration (by the Bio-Rad protein assay reagent), equal volumes of each sample were mixed (1:1 ratio) as indicated and high-molecular-weight complexes were isolated as described above.

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicillin–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. After normalization of the post-nuclear supernatants for equal total protein concentration (by the Bio-Rad protein assay reagent), equal volumes of each sample were mixed (1:1 ratio) as indicated and high-molecular-weight complexes were isolated as described above.

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Following transformation of the pET-CK9 plasmid into BL21 Star (DE3) competent cells (Invitrogen), the induction of recombinant protein production, purification under denaturing conditions, refolding, re-purification under native conditions, SDS-PAGE analysis of proteins and quantification of protein concentration were as described previously [ , , ]. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Sequencing:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: The construct (pET-CK9) encodes an identical CK9 mature amino acid sequence with the addition of methionine at the N-terminus and a his-tag (GSGHHHHHHHH) added at the C-terminus for purification. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Recombinant:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Paragraph title: Production of recombinant CK9 ... The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Molecular Weight:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Thus, the recombinant trout CK9 is 84 aa, with a calculated molecular weight of 9.61 kDa and a theoretical pI of 9.79. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    MTT Assay:

    Article Title: Anti-Inflammatory Effects of 5α,8α-Epidioxycholest-6-en-3β-ol, a Steroidal Endoperoxide Isolated from Aplysia depilans, Based on Bioguided Fractionation and NMR Analysis
    Article Snippet: .. Reagents and Standards LPS from Escherichia coli , sodium pyruvate, sulfanilamide, MTT, Triton X-100, N -(naphth-1-yl)ethylenediamine dihydrochloride, β-nicotinamide adenine dinucleotide reduced form (NADH), sodium deoxycholate, dimethyl sulfoxide (DMSO), trizma hydrochloride, trypan blue, soybean lipoxygenase (LOX) from Glycine max (L.) Merr. (Type V-S; EC 1.13.11.12), phospholipase A2 (PLA2 ) from honey bee (Apis mellifera ) venom (EC.3.1.1.4), propan-2-ol, butan-1-ol, sodium nitroprusside (SNP), l -arginine monohydrochloride, diacetyl monoxime, antipyrine E, H2 SO4 , bovine serum albumin (BSA), chloroform, and N -methyl-l -arginine acetate salt were from Sigma-Aldrich (St. Louis, MO, USA). ..

    Isolation:

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicilli n–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. The cells were grown to 70–90% confluence, and the medium with or without tunicamycin was exchanged 15 h before harvesting, as described above (Isolation of high-molecular-weight complexes).

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicillin–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. The cells were grown to 70–90% confluence, and the medium with or without tunicamycin was exchanged 15 h before harvesting, as described above (Isolation of high-molecular-weight complexes).

    Labeling:

    Article Title: PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
    Article Snippet: .. For Heavy methyl SILAC labeling, HeLa S3 cells were cultured in ‘Light’ and ‘Heavy’ SILAC media (PAA, custom) depleted of lysine, arginine and methionine and supplemented with l -arginine (Sigma Aldrich, A6969) l -lysine (Sigma Aldrich, L8662), and either l -[13 CD3]-methionine (Met-4, heavy, Sigma Aldrich, 299154) or l -[12 CH3]-methionine (Met-0, light, Sigma Aldrich M5308), as previously described ( ). .. For standard SILAC labeling, HeLa cells were grown in ‘‘Light’’ and ‘‘Heavy’’ SILAC DMEM (Thermo Fisher Scientific, 88420) supplemented with either L-arginine and L-lysine, or their heavy isotope-counterparts l -arginine-13 C6 , 15 N4 hydrochloride (Arg10, Sigma, 608033) and L-lysine-13 C6 , 15 N2 hydrochloride (Lys 8, Sigma, 608041) ( ).

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: .. Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicilli n–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either light L-lysine monohydrochloride and L-arginine monohydrochloride (as above) or R10 L-arginine monohydrochloride (referred to as heavy arginine; CK Isotopes, cat. # CNLM-539) and K8 L-lysine monohydrochloride (referred to as heavy lysine; CK Isotopes, cat. # CNLM-291) for several passages ( > 15 cell divisions) before expansion in four 10-cm dishes per sample.

    Article Title: Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site -specific histone methylation
    Article Snippet: Paragraph title: 2.1. Mammalian cell culture and heavy methionine labeling ... 3 Components of homemade medium (all from Sigma-Aldrich): L-Arginine monohydrochloride; L-Cysteine dihydrochloride; L-Histidine dihydrochloride; L-Isoleucine; L-Leucine; L-Lysine monohydrochloride; L-Phenylalanine; L-Threonine; L-Tryptophan; L-Tyrosine; L-Valine; Choline chloride; Folic acid; myo-Inositol; Niacinamide; D-Pantothenic acid hemicalcium salt; Pyridoxal hydrochloride; Riboflavin; Thiamine hydrochloride; MgCl2 ; KCl; NaCl; Na2 HPO4 ; Glucose; Phenol Red sodium salt; NaHCO3 .

    Article Title: AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
    Article Snippet: .. Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in and samples were prepared as follows: Wildtype and FICD-/- CHO-K1 cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco; Life Technologies cat. # 26400-044), 1 x Penicillin-Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma cat. # P5607-25G), 62.5 mg/l “light” L-lysine monohydrochloride (Sigma cat. # L8662-25G) and 60.5 mg/l “light” L-arginine monohydrochloride (Sigma cat. # A6969-25G) and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either 60.5 mg/l “light” (as above) or “heavy” L-arginine monohydrochloride (R10; U-13C6, U-15N4; Cambridge Isotope Laboratories, Inc. cat. # CNLM-539, Andover, MO) for several passages ( > 15 cell divisions) before expansion in five 10 cm dishes per sample.

    Article Title: MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP
    Article Snippet: .. The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in Fig. , and samples were prepared as follows: CHO-K1 S21 wild-type and MANF −/− cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce, cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco, cat. # 26400-044), 1 x Penicillin–Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma, cat. # P5607), 62.5 mg/l L-lysine monohydrochloride (referred to as light lysine; Sigma, cat. # L8662) and 60.5 mg/l L-arginine monohydrochloride (referred to as light arginine; Sigma, cat. # A6969), and incubated as described above. .. Once adapted, the cells were cultured in SILAC medium containing either light L-lysine monohydrochloride and L-arginine monohydrochloride (as above) or R10 L-arginine monohydrochloride (re ferred to as heavy arginine; CK Isotopes, cat. # CNLM-539) and K8 L-lysine monohydrochloride (referred to as heavy lysine; CK Isotopes, cat. # CNLM-291) for several passages ( > 15 cell divisions) before expansion in four 10-cm dishes per sample.

    Article Title: The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module
    Article Snippet: Paragraph title: SILAC Labeling of Dictyostelium Discoideum ... For unlabelled amoebae, cells were grown for the same period by the same methods, with heavy isotope arginine and lysine substituted with standard L-arginine (Sigma A6969) and L-lysine (Sigma L8662) at the same concentrations.

    Purification:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Following transformation of the pET-CK9 plasmid into BL21 Star (DE3) competent cells (Invitrogen), the induction of recombinant protein production, purification under denaturing conditions, refolding, re-purification under native conditions, SDS-PAGE analysis of proteins and quantification of protein concentration were as described previously [ , , ]. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice. Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice
    Article Snippet: .. IL‐6 was infused through a sidearm syringe infusion pump into the arterial supply of the pancreas or the proximal small intestine to reach a final concentration of 100 ng/mL. l ‐Arginine monohydrochloride (10 mmol/L) (Sigma‐Aldrich, Steinheim, Germany, cat.no. .. A6969) dissolved in perfusion buffer was used as a positive control to the perfused pancreas.

    SDS Page:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Following transformation of the pET-CK9 plasmid into BL21 Star (DE3) competent cells (Invitrogen), the induction of recombinant protein production, purification under denaturing conditions, refolding, re-purification under native conditions, SDS-PAGE analysis of proteins and quantification of protein concentration were as described previously [ , , ]. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Plasmid Preparation:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Following transformation of the pET-CK9 plasmid into BL21 Star (DE3) competent cells (Invitrogen), the induction of recombinant protein production, purification under denaturing conditions, refolding, re-purification under native conditions, SDS-PAGE analysis of proteins and quantification of protein concentration were as described previously [ , , ]. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Positron Emission Tomography:

    Article Title: Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
    Article Snippet: Following transformation of the pET-CK9 plasmid into BL21 Star (DE3) competent cells (Invitrogen), the induction of recombinant protein production, purification under denaturing conditions, refolding, re-purification under native conditions, SDS-PAGE analysis of proteins and quantification of protein concentration were as described previously [ , , ]. .. The refolding buffer contained 50 mM Tris-HCl (pH7.5), 10% glycerol, 0.6 M arginine monohydrochloride, 1 M 3-(1-Pyridinio)-1-propanesulfonate (known as NDSB 201 and PPS, Sigma), 0.2% PEG3350 and 5 mM 2-mercaptoethanol.

    Concentration Assay:

    Article Title: Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice. Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice
    Article Snippet: .. IL‐6 was infused through a sidearm syringe infusion pump into the arterial supply of the pancreas or the proximal small intestine to reach a final concentration of 100 ng/mL. l ‐Arginine monohydrochloride (10 mmol/L) (Sigma‐Aldrich, Steinheim, Germany, cat.no. .. A6969) dissolved in perfusion buffer was used as a positive control to the perfused pancreas.

    Article Title: Arginine reprogramming in ADPKD results in arginine-dependent cystogenesis
    Article Snippet: Arginine-defined media were MEK medium prepared with arginine, lysine, and glutamine-free DMEM/F-12 (Caisson Laboratories), supplemented with 0.5 mM lysine (Sigma-Aldrich), 2.5 mM GlutaMAX (ThermoFisher), and various concentrations of l -arginine monohydrochloride (Sigma-Aldrich). .. The concentration of arginine in the lot of FBS used for all experiments was measured to be 3.38 µM, so the actual concentration of arginine in our 0 mM arginine MEK media was 0.07 µM.

    Article Title: p53 Promotes Cancer Cell Adaptation to Glutamine Deprivation by Upregulating Slc7a3 to Increase Arginine Uptake
    Article Snippet: L-Lysine hydrochloride (Sigma L8662) was added to the medium for a final concentration of 0.8 mM. .. Complete DMEM was made by supplementing with 2 mM L-Glutamine (Omega Scientific 61015) and 0.5 mM L-Arginine monohydrochloride (Sigma A6969).

    Lysis:

    Article Title: AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
    Article Snippet: Preparation of SILAC samples The experimental strategy for the stable isotope labeling by amino acids in cell culture (SILAC) experiment is outlined in and samples were prepared as follows: Wildtype and FICD-/- CHO-K1 cells were adapted to Ham’s F12 medium minus L-arginine and L-lysine for SILAC (Pierce cat. # 88424) supplemented with 10% (v/v) dialyzed fetal bovine serum (Gibco; Life Technologies cat. # 26400-044), 1 x Penicillin-Streptomycin (Sigma), 2 mM L-glutamine (Sigma), 280 mg/l L-proline (Sigma cat. # P5607-25G), 62.5 mg/l “light” L-lysine monohydrochloride (Sigma cat. # L8662-25G) and 60.5 mg/l “light” L-arginine monohydrochloride (Sigma cat. # A6969-25G) and incubated as described above. .. After washing with ice-cold PBS the cells were collected in PBS containing 1 mM EDTA and lysed in Triton lysis buffer [20 mM HEPES-KOH pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 10% (v/v) glycerol] containing protease inhibitors.