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TaKaRa platinium taq polymerase
Platinium Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/platinium taq polymerase/product/TaKaRa
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
platinium taq polymerase - by Bioz Stars, 2020-04
85/100 stars

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Related Articles

SYBR Green Assay:

Article Title: Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light 1
Article Snippet: .. Each PCR reaction (50 μ L) contained the following: reverse-transcribed cDNA (1 μ L of 1:5 dilution of the first cDNA strands); PCR buffer (1×), the corresponding primer (each primer 0.4 μ m ), dNTP mix (each 0.25 m m ); MgCl2 (2 m m ), platinium Taq polymerase (1 unit; CLONTECH), and SYBR green I (1/1,000; Sigma). .. After a heat step at 94°C for 4 min, PCR cycling conditions were 35 cycles of denaturation (94°C, 15 s), annealing (58.5°C, 15 s), and elongation (72°C, 20 s).

Amplification:

Article Title: Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light 1
Article Snippet: Expression analysis of two aquaporin isoforms (JrPIP2,1 and JrPIP2,2) was done by real-time RT-PCR amplification, using an iCycler iQ (Bio-Rad Laboratories) and SYBR green as a fluorescent dye. .. Each PCR reaction (50 μ L) contained the following: reverse-transcribed cDNA (1 μ L of 1:5 dilution of the first cDNA strands); PCR buffer (1×), the corresponding primer (each primer 0.4 μ m ), dNTP mix (each 0.25 m m ); MgCl2 (2 m m ), platinium Taq polymerase (1 unit; CLONTECH), and SYBR green I (1/1,000; Sigma).

Quantitative RT-PCR:

Article Title: Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light 1
Article Snippet: Paragraph title: Real-Time Quantitative RT-PCR Experiments ... Each PCR reaction (50 μ L) contained the following: reverse-transcribed cDNA (1 μ L of 1:5 dilution of the first cDNA strands); PCR buffer (1×), the corresponding primer (each primer 0.4 μ m ), dNTP mix (each 0.25 m m ); MgCl2 (2 m m ), platinium Taq polymerase (1 unit; CLONTECH), and SYBR green I (1/1,000; Sigma).

Real-time Polymerase Chain Reaction:

Article Title: Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light 1
Article Snippet: Each PCR reaction (50 μ L) contained the following: reverse-transcribed cDNA (1 μ L of 1:5 dilution of the first cDNA strands); PCR buffer (1×), the corresponding primer (each primer 0.4 μ m ), dNTP mix (each 0.25 m m ); MgCl2 (2 m m ), platinium Taq polymerase (1 unit; CLONTECH), and SYBR green I (1/1,000; Sigma). .. Because the primer pairs WC11/WC12 and WC21/WC22 were initially shown to be specific for JrPIP2,1 and JrPIP2,2, respectively , they were used for this real-time PCR.

Expressing:

Article Title: Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light 1
Article Snippet: Expression analysis of two aquaporin isoforms (JrPIP2,1 and JrPIP2,2) was done by real-time RT-PCR amplification, using an iCycler iQ (Bio-Rad Laboratories) and SYBR green as a fluorescent dye. .. Each PCR reaction (50 μ L) contained the following: reverse-transcribed cDNA (1 μ L of 1:5 dilution of the first cDNA strands); PCR buffer (1×), the corresponding primer (each primer 0.4 μ m ), dNTP mix (each 0.25 m m ); MgCl2 (2 m m ), platinium Taq polymerase (1 unit; CLONTECH), and SYBR green I (1/1,000; Sigma).

Polymerase Chain Reaction:

Article Title: Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light 1
Article Snippet: .. Each PCR reaction (50 μ L) contained the following: reverse-transcribed cDNA (1 μ L of 1:5 dilution of the first cDNA strands); PCR buffer (1×), the corresponding primer (each primer 0.4 μ m ), dNTP mix (each 0.25 m m ); MgCl2 (2 m m ), platinium Taq polymerase (1 unit; CLONTECH), and SYBR green I (1/1,000; Sigma). .. After a heat step at 94°C for 4 min, PCR cycling conditions were 35 cycles of denaturation (94°C, 15 s), annealing (58.5°C, 15 s), and elongation (72°C, 20 s).

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