plasmid vector pstblue 1  (Millipore)


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    Structured Review

    Millipore plasmid vector pstblue 1
    Plasmid Vector Pstblue 1, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid vector pstblue 1/product/Millipore
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    plasmid vector pstblue 1 - by Bioz Stars, 2020-08
    85/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET30b (Novagen). .. DNA fragments encoding PrcB, the N-terminal region of PrcA (PrcA1 polypeptide), and the N-terminal region of PrtP were amplified by PCR from T. denticola genomic DNA, initially cloned in pSTBlue-1 and maintained in E. coli JM109.

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen). .. Plasmid pCTLP ( ) carries a 4-kb ORF2- prtP fragment from T. denticola ATCC 35405.

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of polymerase chain reaction (PCR) products, and 6xHis-tagged constructs were made in pET28b (Novagen, Inc., Madison, WI, USA). .. DNA encoding the C-terminal region of prtP was amplified from T. denticola genomic DNA using primers CX616 and CX822 , and the resulting PCR product carrying 5′ NcoI and 3′ XhoI engineered restriction sites was cloned in pET28b (Novagen) such that in the resulting plasmid (pCF617), a partial prtP open reading frame including a C-terminal 6xHistidine tag (6xHis) was expressed from the vector-encoded T7 promoter.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen). .. A 1,070-bp fragment of T. denticola genomic DNA containing the entire prcB ORF and approximately 500 bp 5′ of prcB was amplified by a PCR using oligonucleotide primers CX516 and CX529. (Details of all plasmids and oligonucleotide primers used in this study are contained in Tables and .)

    Clone Assay:

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET30b (Novagen). .. DNA fragments encoding PrcB, the N-terminal region of PrcA (PrcA1 polypeptide), and the N-terminal region of PrtP were amplified by PCR from T. denticola genomic DNA, initially cloned in pSTBlue-1 and maintained in E. coli JM109.

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen). .. Plasmid pCTLP ( ) carries a 4-kb ORF2- prtP fragment from T. denticola ATCC 35405.

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of polymerase chain reaction (PCR) products, and 6xHis-tagged constructs were made in pET28b (Novagen, Inc., Madison, WI, USA). .. DNA encoding the C-terminal region of prtP was amplified from T. denticola genomic DNA using primers CX616 and CX822 , and the resulting PCR product carrying 5′ NcoI and 3′ XhoI engineered restriction sites was cloned in pET28b (Novagen) such that in the resulting plasmid (pCF617), a partial prtP open reading frame including a C-terminal 6xHistidine tag (6xHis) was expressed from the vector-encoded T7 promoter.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen). .. A 1,070-bp fragment of T. denticola genomic DNA containing the entire prcB ORF and approximately 500 bp 5′ of prcB was amplified by a PCR using oligonucleotide primers CX516 and CX529. (Details of all plasmids and oligonucleotide primers used in this study are contained in Tables and .)

    Plasmid Preparation:

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET30b (Novagen). .. DNA fragments encoding PrcB, the N-terminal region of PrcA (PrcA1 polypeptide), and the N-terminal region of PrtP were amplified by PCR from T. denticola genomic DNA, initially cloned in pSTBlue-1 and maintained in E. coli JM109.

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. E. coli NovaBlue (Novagen) was used as a host strain for direct cloning of PCR fragments in plasmid vector pSTBlue-1 (Novagen). .. Plasmid pCTLP ( ) carries a 4-kb ORF2- prtP fragment from T. denticola ATCC 35405.

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of polymerase chain reaction (PCR) products, and 6xHis-tagged constructs were made in pET28b (Novagen, Inc., Madison, WI, USA). .. DNA encoding the C-terminal region of prtP was amplified from T. denticola genomic DNA using primers CX616 and CX822 , and the resulting PCR product carrying 5′ NcoI and 3′ XhoI engineered restriction sites was cloned in pET28b (Novagen) such that in the resulting plasmid (pCF617), a partial prtP open reading frame including a C-terminal 6xHistidine tag (6xHis) was expressed from the vector-encoded T7 promoter.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen). .. A 1,070-bp fragment of T. denticola genomic DNA containing the entire prcB ORF and approximately 500 bp 5′ of prcB was amplified by a PCR using oligonucleotide primers CX516 and CX529. (Details of all plasmids and oligonucleotide primers used in this study are contained in Tables and .)

    Article Title: A simplified erythromycin resistance cassette for Treponema denticola mutagenesis
    Article Snippet: .. The resulting plasmids, based on plasmid vector pSTBlue-1 (Novagen, Inc., Madison, WI, USA)), conferred both kanamycin resistance (30 μg ml−1 ) and erythromycin (300 μg ml−1 ) in the E. coli JM109 host. .. Plasmids carrying the mutant constructs were linearized to facilitate allelic replacement by homologous recombination.

    Construct:

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET30b (Novagen). .. DNA fragments encoding PrcB, the N-terminal region of PrcA (PrcA1 polypeptide), and the N-terminal region of PrtP were amplified by PCR from T. denticola genomic DNA, initially cloned in pSTBlue-1 and maintained in E. coli JM109.

    Article Title: Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of polymerase chain reaction (PCR) products, and 6xHis-tagged constructs were made in pET28b (Novagen, Inc., Madison, WI, USA). .. DNA encoding the C-terminal region of prtP was amplified from T. denticola genomic DNA using primers CX616 and CX822 , and the resulting PCR product carrying 5′ NcoI and 3′ XhoI engineered restriction sites was cloned in pET28b (Novagen) such that in the resulting plasmid (pCF617), a partial prtP open reading frame including a C-terminal 6xHistidine tag (6xHis) was expressed from the vector-encoded T7 promoter.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen). .. A 1,070-bp fragment of T. denticola genomic DNA containing the entire prcB ORF and approximately 500 bp 5′ of prcB was amplified by a PCR using oligonucleotide primers CX516 and CX529. (Details of all plasmids and oligonucleotide primers used in this study are contained in Tables and .)

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  • 85
    Millipore pstblue 1 acceptor
    Pstblue 1 Acceptor, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pstblue 1 acceptor/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pstblue 1 acceptor - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Millipore pstblue 1 acceptortm vector
    Pstblue 1 Acceptortm Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pstblue 1 acceptortm vector/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pstblue 1 acceptortm vector - by Bioz Stars, 2020-08
    93/100 stars
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    85
    Millipore pstblue 1 acceptor vector kit
    Pstblue 1 Acceptor Vector Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pstblue 1 acceptor vector kit/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pstblue 1 acceptor vector kit - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    89
    Millipore pstblue 1 vector
    Pstblue 1 Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pstblue 1 vector/product/Millipore
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    pstblue 1 vector - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

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