plasmid miniprep classic kit  (Zymo Research)


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    Name:
    ZR Plasmid Miniprep Classic
    Description:
    The ZR Plasmid Miniprep Classic is designed for efficient isolation of plasmid DNA from E Coli using a traditional 3 buffer P1 P2 P3 procedure that is simple rapid and reliable It features a modified alkaline lysis protocol together with Zymo Spin technology to yield high quality plasmid DNA in minutes The buffers are color coded red green yellow for easy visualization of complete cell lysis and neutralization The innovative Zymo Spin llN columns yield endotoxin free plasmid DNA Plasmid DNA purified using the ZR Plasmid Miniprep Classic is well suited for use in restriction endonuclease digestion sequencing DNA ligation cloning PCR bacterial transformation transfection etc
    Catalog Number:
    d4016
    Price:
    None
    Applications:
    Plasmid DNA Purification
    Size:
    100 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research plasmid miniprep classic kit
    ZR Plasmid Miniprep Classic
    The ZR Plasmid Miniprep Classic is designed for efficient isolation of plasmid DNA from E Coli using a traditional 3 buffer P1 P2 P3 procedure that is simple rapid and reliable It features a modified alkaline lysis protocol together with Zymo Spin technology to yield high quality plasmid DNA in minutes The buffers are color coded red green yellow for easy visualization of complete cell lysis and neutralization The innovative Zymo Spin llN columns yield endotoxin free plasmid DNA Plasmid DNA purified using the ZR Plasmid Miniprep Classic is well suited for use in restriction endonuclease digestion sequencing DNA ligation cloning PCR bacterial transformation transfection etc
    https://www.bioz.com/result/plasmid miniprep classic kit/product/Zymo Research
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    plasmid miniprep classic kit - by Bioz Stars, 2020-08
    95/100 stars

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    plasmid dna

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    Related Articles

    Clone Assay:

    Article Title: PK11195 Effect on Steroidogenesis Is Not Mediated Through the Translocator Protein (TSPO)
    Article Snippet: .. Three MA-10: Tspo Δ/Δ cell clones were identified and mutations in the Tspo gene were confirmed by sequencing after cloning both the respective Tspo cDNAs and Tspo exon 2 genomic DNA into TOPO®-TA vectors (Life Technologies), and ZR Plasmid Miniprep (Zymo Research) purification. .. Cells were incubated with 300nM Mitotracker Deep Red FM (Life Technologies) in culture medium for 1 hour at 37°C, washed with PBS, and fixed with 2% paraformaldehyde.

    Article Title: Production of Putative Diterpene Carboxylic Acid Intermediates of Triptolide in Yeast
    Article Snippet: .. Cloned constructs were transformed into Mix and Go® X10 Gold Escherichia coli competent cells (Zymo Research Europe GmbH, Feiburg im Breisgau, Germany) following standard protocol and plasmids were recovered using a ZR Plasmid Miniprep™—Classic (Zymo Research Europe GmbH) following included protocol. .. Purity and concentration were controlled by NanoDrop2000 (Thermofisher Scientific) and all constructs were sequence verified.

    Article Title: Constitutive promoter methylation of BRCA1 and RAD51C in patients with familial ovarian cancer and early-onset sporadic breast cancer
    Article Snippet: .. Plasmid DNA of individual clones was isolated with the ZR Plasmid Miniprep Classic Kit (Zymo Research). .. Clones containing inserts of the right size were sequenced using dye terminator cycle sequencing with M13 primers on an ABI 3730 automated sequencer.

    Isolation:

    Article Title: Constitutive promoter methylation of BRCA1 and RAD51C in patients with familial ovarian cancer and early-onset sporadic breast cancer
    Article Snippet: .. Plasmid DNA of individual clones was isolated with the ZR Plasmid Miniprep Classic Kit (Zymo Research). .. Clones containing inserts of the right size were sequenced using dye terminator cycle sequencing with M13 primers on an ABI 3730 automated sequencer.

    Article Title: Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Sequence Type 95 Strains
    Article Snippet: .. Plasmid DNA was isolated from E. coli cultures using the ZR Plasmid Miniprep Classic kit (Zymo Research). .. Plasmid DNA was analyzed on 1% agarose gels.

    Genomic Sequencing:

    Article Title: miR-23b/SP1/c-myc forms a feed-forward loop supporting multiple myeloma cell growth
    Article Snippet: .. The pGL3/miR-23b promoter plasmid was purified using ZR Plasmid Miniprep Classic (Zymo Research) and analyzed by automated sequencing in ABI PRISM 310 with the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) to confirm that the sequence matched the original genomic sequences without PCR-generated errors. .. Quantitative DNA methylation analysisPrimers for quantitative DNA methylation experiments were designed by using EpiDesigner, a specialized tool for Sequenom'sEpiTYPER technology.

    Construct:

    Article Title: Production of Putative Diterpene Carboxylic Acid Intermediates of Triptolide in Yeast
    Article Snippet: .. Cloned constructs were transformed into Mix and Go® X10 Gold Escherichia coli competent cells (Zymo Research Europe GmbH, Feiburg im Breisgau, Germany) following standard protocol and plasmids were recovered using a ZR Plasmid Miniprep™—Classic (Zymo Research Europe GmbH) following included protocol. .. Purity and concentration were controlled by NanoDrop2000 (Thermofisher Scientific) and all constructs were sequence verified.

    Purification:

    Article Title: PK11195 Effect on Steroidogenesis Is Not Mediated Through the Translocator Protein (TSPO)
    Article Snippet: .. Three MA-10: Tspo Δ/Δ cell clones were identified and mutations in the Tspo gene were confirmed by sequencing after cloning both the respective Tspo cDNAs and Tspo exon 2 genomic DNA into TOPO®-TA vectors (Life Technologies), and ZR Plasmid Miniprep (Zymo Research) purification. .. Cells were incubated with 300nM Mitotracker Deep Red FM (Life Technologies) in culture medium for 1 hour at 37°C, washed with PBS, and fixed with 2% paraformaldehyde.

    Article Title: Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis
    Article Snippet: .. Plasmids were purified using a ZR Plasmid Miniprep-Classic kit (Zymo Research, Freiburg, Germany) and commercial sequencing was used to verify the sequences (LGC genomics, Berlin, Germany). .. The homotetrameric GDH-SC (41 kDa per monomer) was cloned as previously described [ ].

    Article Title: miR-23b/SP1/c-myc forms a feed-forward loop supporting multiple myeloma cell growth
    Article Snippet: .. The pGL3/miR-23b promoter plasmid was purified using ZR Plasmid Miniprep Classic (Zymo Research) and analyzed by automated sequencing in ABI PRISM 310 with the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) to confirm that the sequence matched the original genomic sequences without PCR-generated errors. .. Quantitative DNA methylation analysisPrimers for quantitative DNA methylation experiments were designed by using EpiDesigner, a specialized tool for Sequenom'sEpiTYPER technology.

    Article Title: Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors
    Article Snippet: .. Plasmid DNA was purified with ZR Plasmid MiniprepTM —Classic (Zymo Research Corp., Irvine, CA, USA). .. The nucleotide sequence of the cloned fragments was verified by sequencing.

    Sequencing:

    Article Title: PK11195 Effect on Steroidogenesis Is Not Mediated Through the Translocator Protein (TSPO)
    Article Snippet: .. Three MA-10: Tspo Δ/Δ cell clones were identified and mutations in the Tspo gene were confirmed by sequencing after cloning both the respective Tspo cDNAs and Tspo exon 2 genomic DNA into TOPO®-TA vectors (Life Technologies), and ZR Plasmid Miniprep (Zymo Research) purification. .. Cells were incubated with 300nM Mitotracker Deep Red FM (Life Technologies) in culture medium for 1 hour at 37°C, washed with PBS, and fixed with 2% paraformaldehyde.

    Article Title: Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis
    Article Snippet: .. Plasmids were purified using a ZR Plasmid Miniprep-Classic kit (Zymo Research, Freiburg, Germany) and commercial sequencing was used to verify the sequences (LGC genomics, Berlin, Germany). .. The homotetrameric GDH-SC (41 kDa per monomer) was cloned as previously described [ ].

    Article Title: miR-23b/SP1/c-myc forms a feed-forward loop supporting multiple myeloma cell growth
    Article Snippet: .. The pGL3/miR-23b promoter plasmid was purified using ZR Plasmid Miniprep Classic (Zymo Research) and analyzed by automated sequencing in ABI PRISM 310 with the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) to confirm that the sequence matched the original genomic sequences without PCR-generated errors. .. Quantitative DNA methylation analysisPrimers for quantitative DNA methylation experiments were designed by using EpiDesigner, a specialized tool for Sequenom'sEpiTYPER technology.

    Polymerase Chain Reaction:

    Article Title: miR-23b/SP1/c-myc forms a feed-forward loop supporting multiple myeloma cell growth
    Article Snippet: .. The pGL3/miR-23b promoter plasmid was purified using ZR Plasmid Miniprep Classic (Zymo Research) and analyzed by automated sequencing in ABI PRISM 310 with the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) to confirm that the sequence matched the original genomic sequences without PCR-generated errors. .. Quantitative DNA methylation analysisPrimers for quantitative DNA methylation experiments were designed by using EpiDesigner, a specialized tool for Sequenom'sEpiTYPER technology.

    Transformation Assay:

    Article Title: Production of Putative Diterpene Carboxylic Acid Intermediates of Triptolide in Yeast
    Article Snippet: .. Cloned constructs were transformed into Mix and Go® X10 Gold Escherichia coli competent cells (Zymo Research Europe GmbH, Feiburg im Breisgau, Germany) following standard protocol and plasmids were recovered using a ZR Plasmid Miniprep™—Classic (Zymo Research Europe GmbH) following included protocol. .. Purity and concentration were controlled by NanoDrop2000 (Thermofisher Scientific) and all constructs were sequence verified.

    Plasmid Preparation:

    Article Title: PK11195 Effect on Steroidogenesis Is Not Mediated Through the Translocator Protein (TSPO)
    Article Snippet: .. Three MA-10: Tspo Δ/Δ cell clones were identified and mutations in the Tspo gene were confirmed by sequencing after cloning both the respective Tspo cDNAs and Tspo exon 2 genomic DNA into TOPO®-TA vectors (Life Technologies), and ZR Plasmid Miniprep (Zymo Research) purification. .. Cells were incubated with 300nM Mitotracker Deep Red FM (Life Technologies) in culture medium for 1 hour at 37°C, washed with PBS, and fixed with 2% paraformaldehyde.

    Article Title: Imine Reductase Based All-Enzyme Hydrogel with Intrinsic Cofactor Regeneration for Flow Biocatalysis
    Article Snippet: .. Plasmids were purified using a ZR Plasmid Miniprep-Classic kit (Zymo Research, Freiburg, Germany) and commercial sequencing was used to verify the sequences (LGC genomics, Berlin, Germany). .. The homotetrameric GDH-SC (41 kDa per monomer) was cloned as previously described [ ].

    Article Title: miR-23b/SP1/c-myc forms a feed-forward loop supporting multiple myeloma cell growth
    Article Snippet: .. The pGL3/miR-23b promoter plasmid was purified using ZR Plasmid Miniprep Classic (Zymo Research) and analyzed by automated sequencing in ABI PRISM 310 with the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) to confirm that the sequence matched the original genomic sequences without PCR-generated errors. .. Quantitative DNA methylation analysisPrimers for quantitative DNA methylation experiments were designed by using EpiDesigner, a specialized tool for Sequenom'sEpiTYPER technology.

    Article Title: Production of Putative Diterpene Carboxylic Acid Intermediates of Triptolide in Yeast
    Article Snippet: .. Cloned constructs were transformed into Mix and Go® X10 Gold Escherichia coli competent cells (Zymo Research Europe GmbH, Feiburg im Breisgau, Germany) following standard protocol and plasmids were recovered using a ZR Plasmid Miniprep™—Classic (Zymo Research Europe GmbH) following included protocol. .. Purity and concentration were controlled by NanoDrop2000 (Thermofisher Scientific) and all constructs were sequence verified.

    Article Title: A quasi-integral controller for adaptation of genetic modules to variable ribosome demand
    Article Snippet: .. Plasmid DNA was prepared by the plasmid miniprep-classic kit (Zymo Research, D4015). .. DNA sequencing used Quintarabio DNA basic sequencing service.

    Article Title: Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors
    Article Snippet: .. Plasmid DNA was purified with ZR Plasmid MiniprepTM —Classic (Zymo Research Corp., Irvine, CA, USA). .. The nucleotide sequence of the cloned fragments was verified by sequencing.

    Article Title: Constitutive promoter methylation of BRCA1 and RAD51C in patients with familial ovarian cancer and early-onset sporadic breast cancer
    Article Snippet: .. Plasmid DNA of individual clones was isolated with the ZR Plasmid Miniprep Classic Kit (Zymo Research). .. Clones containing inserts of the right size were sequenced using dye terminator cycle sequencing with M13 primers on an ABI 3730 automated sequencer.

    Article Title: Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Sequence Type 95 Strains
    Article Snippet: .. Plasmid DNA was isolated from E. coli cultures using the ZR Plasmid Miniprep Classic kit (Zymo Research). .. Plasmid DNA was analyzed on 1% agarose gels.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Zymo Research zr plasmid miniprep classic kit
    Zr Plasmid Miniprep Classic Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zr plasmid miniprep classic kit/product/Zymo Research
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    zr plasmid miniprep classic kit - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

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