plasmid giga kit  (Qiagen)


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    Structured Review

    Qiagen plasmid giga kit
    Plasmid Giga Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid giga kit/product/Qiagen
    Average 95 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    plasmid giga kit - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Briefly, the N gene was cloned in the pBlueBacHis2A vector (Invitrogen).

    Transfection:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Recombinant baculovirus expressing the N protein (BacN) was obtained after transfection of Sf9 insect cells with the recombinant plasmid and purification of the ␤-galactosidaseexpressing plaques as recommended by the manufacturer.

    Affinity Chromatography:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: .. Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Expression of the N protein The purified N protein was obtained from a recombinant baculovirus expressing the coding sequence of the N protein of BRSV strain RB94.

    Construct:

    Article Title: Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly.
    Article Snippet: The HBV S/V1012 plasmid (CMV promoter, 5.7 kb), pLuc/V1012 plasmid (CMV promoter, 6.4 kb) and pEGFP/V1012 plasmid (CMV promoter, 5.8 kb) were constructed by our Lab and obtained by transforming Escherichia coli strain top10 and cultured under defined growth conditions. .. The plasmids were purified by using a Qiagen Plasmid Giga kit, and the final product was endotoxin free (o2.5 units/mg).

    Purification:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: .. Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Expression of the N protein The purified N protein was obtained from a recombinant baculovirus expressing the coding sequence of the N protein of BRSV strain RB94.

    Article Title: Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly.
    Article Snippet: .. The plasmids were purified by using a Qiagen Plasmid Giga kit, and the final product was endotoxin free (o2.5 units/mg). ..

    Plasmid Preparation:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: .. Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Expression of the N protein The purified N protein was obtained from a recombinant baculovirus expressing the coding sequence of the N protein of BRSV strain RB94.

    Article Title: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
    Article Snippet: .. S R1-3 are different recombinants representing the putative hydrophilic regions. used to immunize animals was prepared by Plasmid giga kit from Qiagen (Valencia, CA) according to manufacturer's instructions. ..

    Article Title: Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly.
    Article Snippet: .. The plasmids were purified by using a Qiagen Plasmid Giga kit, and the final product was endotoxin free (o2.5 units/mg). ..

    Sequencing:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: Expression of the N protein The pcDNA3 plasmid expressing synthetic DNA sequence coding for the ORF of the N protein of BRSV (pNsyn) has been described elsewhere [39] . .. Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen).

    Article Title: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
    Article Snippet: Signal sequence of S gene is marked as a solid black box and the putative highly hydrophobic regions of S1 are shown in solid box in light grey color. .. S R1-3 are different recombinants representing the putative hydrophilic regions. used to immunize animals was prepared by Plasmid giga kit from Qiagen (Valencia, CA) according to manufacturer's instructions.

    Cell Culture:

    Article Title: Enhance immune response to DNA vaccine based on a novel multicomponent supramolecular assembly.
    Article Snippet: The HBV S/V1012 plasmid (CMV promoter, 5.7 kb), pLuc/V1012 plasmid (CMV promoter, 6.4 kb) and pEGFP/V1012 plasmid (CMV promoter, 5.8 kb) were constructed by our Lab and obtained by transforming Escherichia coli strain top10 and cultured under defined growth conditions. .. The plasmids were purified by using a Qiagen Plasmid Giga kit, and the final product was endotoxin free (o2.5 units/mg).

    Expressing:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: Paragraph title: Expression of the N protein ... Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen).

    Article Title: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
    Article Snippet: All the fragments including the recombinant fragments were cloned into mammalian expression vector pCI-SP pGH , except for S La and S Na which were cloned in pCI. .. S R1-3 are different recombinants representing the putative hydrophilic regions. used to immunize animals was prepared by Plasmid giga kit from Qiagen (Valencia, CA) according to manufacturer's instructions.

    Modification:

    Article Title: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
    Article Snippet: S R1-3 are different recombinants representing the putative hydrophilic regions. used to immunize animals was prepared by Plasmid giga kit from Qiagen (Valencia, CA) according to manufacturer's instructions. .. The vector was modified by adding the 6ÂHis and IRES-GFP up-and downstream of the multiple cloning sites of pcDNA3.1(+), respectively.

    Transformation Assay:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: .. Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Expression of the N protein The purified N protein was obtained from a recombinant baculovirus expressing the coding sequence of the N protein of BRSV strain RB94.

    Recombinant:

    Article Title: Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
    Article Snippet: Large scale plasmid DNA was purified from transformed TOP 10 bacteria cells by affinity chromatography on anion-exchange resin (Plasmid Giga kit, Qiagen). .. Expression of the N protein The purified N protein was obtained from a recombinant baculovirus expressing the coding sequence of the N protein of BRSV strain RB94.

    Article Title: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
    Article Snippet: All the fragments including the recombinant fragments were cloned into mammalian expression vector pCI-SP pGH , except for S La and S Na which were cloned in pCI. .. S R1-3 are different recombinants representing the putative hydrophilic regions. used to immunize animals was prepared by Plasmid giga kit from Qiagen (Valencia, CA) according to manufacturer's instructions.

    Derivative Assay:

    Article Title: Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
    Article Snippet: S R1-3 are different recombinants representing the putative hydrophilic regions. used to immunize animals was prepared by Plasmid giga kit from Qiagen (Valencia, CA) according to manufacturer's instructions. .. Materials and methods To test the expression of S gene and its derived fragments in mammalian cells, all the DNA fragments, as shown in Fig. 1 , were cloned into pcDNA3.1-6ÂHis-IRES-GFP through BamHI and EcoRI sites.

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  • 99
    Qiagen endo free plasmid giga kit
    Endo Free Plasmid Giga Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endo free plasmid giga kit/product/Qiagen
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    endo free plasmid giga kit - by Bioz Stars, 2020-04
    99/100 stars
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