Structured Review

Genomed GmbH plasmid dna
Plasmid Dna, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna/product/Genomed GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
plasmid dna - by Bioz Stars, 2021-03
86/100 stars

Images

Related Articles

Plasmid Preparation:

Article Title: Functional relevance of aromatic residues in the first transmembrane domain of P2X receptors
Article Snippet: Previous experiments showed that this receptor responds to ATP with a sensitivity and peak amplitude of current highly comparable to those observed in cells expressing the non-tagged WT receptor ( ). .. Plasmid DNAs for transfection were purified using a Jetquick plasmid spin kit (Genomed, Löhne, Germany). .. The presence of the mutations and the identity of all constructs were verified by dye terminator cycle sequencing (PerkinElmer Life and Analytical Sciences, Boston MA - performed by the Laboratory of Molecular Technology, NCI, Frederick, MD and Veritas, Inc. Rockville, MD; ABI PRISM 3100, Applied Biosystems, Foster City, CA - performed by the Laboratory of DNA sequencing, Institute of Microbiology, ASCR, Prague).

Article Title: Cortactin Tyrosine Phosphorylation Promotes Its Deacetylation and Inhibits Cell Spreading
Article Snippet: WT GFP-cortactin and 3F-GFP constructs were previously described . .. Cell transfection and Western blotting (WB) For transfection, plasmid DNAs were purified with endotoxin-free, transfection-grade JetStar 2.0 Midi columns (Genomed) as per the manufacturer's instructions. .. Cell transfections were carried out using Lipofectamine 2000 (Invitrogen) or Fugene HD transfection reagent (Roche).

Article Title: Epigenetic Modifications and Canonical Wingless/int-1 Class (WNT) Signaling Enable Trans-differentiation of Nonosteogenic Cells into Osteoblasts *
Article Snippet: Expression of Lef-1 and Dlx5 proteins from their respective vectors were each confirmed by Western blot previously ( , ). .. All plasmid DNAs were prepared using a DNA Maxi-prep kit (Genomed, Loehne, Germany). .. C2C12 cells were plated in 100-mm plates and cultured up to 90–100% confluency.

Article Title: Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma
Article Snippet: The expression of the shRNA molecule from the plasmid DNA in the cells was controlled by the human U6 promoter. .. The plasmid DNA was amplified in E. coli (TOP10; Invitrogen) and isolated using a NoEndo JETSTAR ENDOTOXIN-FREE MEGA/GIGA Kit (Genomed, Löhne, DE) according to the manufacturer's protocol. .. The quantity of isolated plasmid DNA was determined by a spectrophotometer at 260 nm (Epoch Microplate Spectrophotometer, Take3 Micro-Volume Plate, BioTek, Bad Friedrichshall, DE) and the quality by measuring the ratio of absorbance at A260 nm/280 nm and by agarose gel electrophoresis.

Article Title: The P450–1 gene of Gibberella fujikuroi encodes a multifunctional enzyme in gibberellin biosynthesis
Article Snippet: Genomic DNA was isolated from lyophilized mycelium according to ref. . .. Plasmid DNA was extracted by using Genomed columns following the manufacturer's protocol (Genomed, Bad Oeynhausen, Germany). .. After endonuclease digestion and electrophoresis, genomic DNA was transferred onto Hybond N+ filters (Amersham Pharmacia).

Article Title: Characterization of a Salt-Tolerant Family 42 ?-Galactosidase from a Psychrophilic Antarctic Planococcus Isolate
Article Snippet: .. Plasmid DNA from one transformant was purified by using a Genomed maxi prep kit (PGC, Gaithersburg, Md.). .. The gene encoding the β-galactosidase activity was sequenced at the Penn State Nucleic Acid Facility with an ABI model 370 sequencer.

Article Title: Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein
Article Snippet: The Bac-rNV C8 (ORFs 2 and 3) used in these experiments was generated and described previously ( ). .. Plasmid DNA was linearized and purified on an agarose gel (Jetsorb, Genomed; PGC, Gaithersburg, Md.). .. The purified DNA was transcribed in vitro with SP6 or T7 polymerase using the MaxiScript kit (Ambion, Austin, Tex.).

Article Title: Mutations Which Uncouple Transport and Phosphorylation in the d-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14
Article Snippet: All manipulations with recombinant DNA were performed by using standard procedures ( ). .. Plasmid DNA was prepared by the standard phenol extraction procedure ( ) or by using the JETstar DNA purification system (Genomed, Bad Oeynhausen, Germany). .. Restriction enzymes were purchased from New England Biolabs (Schwalbach, Germany) and were used according to the recommendations of the supplier.

Transfection:

Article Title: Functional relevance of aromatic residues in the first transmembrane domain of P2X receptors
Article Snippet: Previous experiments showed that this receptor responds to ATP with a sensitivity and peak amplitude of current highly comparable to those observed in cells expressing the non-tagged WT receptor ( ). .. Plasmid DNAs for transfection were purified using a Jetquick plasmid spin kit (Genomed, Löhne, Germany). .. The presence of the mutations and the identity of all constructs were verified by dye terminator cycle sequencing (PerkinElmer Life and Analytical Sciences, Boston MA - performed by the Laboratory of Molecular Technology, NCI, Frederick, MD and Veritas, Inc. Rockville, MD; ABI PRISM 3100, Applied Biosystems, Foster City, CA - performed by the Laboratory of DNA sequencing, Institute of Microbiology, ASCR, Prague).

Article Title: Cortactin Tyrosine Phosphorylation Promotes Its Deacetylation and Inhibits Cell Spreading
Article Snippet: WT GFP-cortactin and 3F-GFP constructs were previously described . .. Cell transfection and Western blotting (WB) For transfection, plasmid DNAs were purified with endotoxin-free, transfection-grade JetStar 2.0 Midi columns (Genomed) as per the manufacturer's instructions. .. Cell transfections were carried out using Lipofectamine 2000 (Invitrogen) or Fugene HD transfection reagent (Roche).

Purification:

Article Title: Functional relevance of aromatic residues in the first transmembrane domain of P2X receptors
Article Snippet: Previous experiments showed that this receptor responds to ATP with a sensitivity and peak amplitude of current highly comparable to those observed in cells expressing the non-tagged WT receptor ( ). .. Plasmid DNAs for transfection were purified using a Jetquick plasmid spin kit (Genomed, Löhne, Germany). .. The presence of the mutations and the identity of all constructs were verified by dye terminator cycle sequencing (PerkinElmer Life and Analytical Sciences, Boston MA - performed by the Laboratory of Molecular Technology, NCI, Frederick, MD and Veritas, Inc. Rockville, MD; ABI PRISM 3100, Applied Biosystems, Foster City, CA - performed by the Laboratory of DNA sequencing, Institute of Microbiology, ASCR, Prague).

Article Title: Cortactin Tyrosine Phosphorylation Promotes Its Deacetylation and Inhibits Cell Spreading
Article Snippet: WT GFP-cortactin and 3F-GFP constructs were previously described . .. Cell transfection and Western blotting (WB) For transfection, plasmid DNAs were purified with endotoxin-free, transfection-grade JetStar 2.0 Midi columns (Genomed) as per the manufacturer's instructions. .. Cell transfections were carried out using Lipofectamine 2000 (Invitrogen) or Fugene HD transfection reagent (Roche).

Article Title: Characterization of a Salt-Tolerant Family 42 ?-Galactosidase from a Psychrophilic Antarctic Planococcus Isolate
Article Snippet: .. Plasmid DNA from one transformant was purified by using a Genomed maxi prep kit (PGC, Gaithersburg, Md.). .. The gene encoding the β-galactosidase activity was sequenced at the Penn State Nucleic Acid Facility with an ABI model 370 sequencer.

Article Title: Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein
Article Snippet: The Bac-rNV C8 (ORFs 2 and 3) used in these experiments was generated and described previously ( ). .. Plasmid DNA was linearized and purified on an agarose gel (Jetsorb, Genomed; PGC, Gaithersburg, Md.). .. The purified DNA was transcribed in vitro with SP6 or T7 polymerase using the MaxiScript kit (Ambion, Austin, Tex.).

Western Blot:

Article Title: Cortactin Tyrosine Phosphorylation Promotes Its Deacetylation and Inhibits Cell Spreading
Article Snippet: WT GFP-cortactin and 3F-GFP constructs were previously described . .. Cell transfection and Western blotting (WB) For transfection, plasmid DNAs were purified with endotoxin-free, transfection-grade JetStar 2.0 Midi columns (Genomed) as per the manufacturer's instructions. .. Cell transfections were carried out using Lipofectamine 2000 (Invitrogen) or Fugene HD transfection reagent (Roche).

Amplification:

Article Title: Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma
Article Snippet: The expression of the shRNA molecule from the plasmid DNA in the cells was controlled by the human U6 promoter. .. The plasmid DNA was amplified in E. coli (TOP10; Invitrogen) and isolated using a NoEndo JETSTAR ENDOTOXIN-FREE MEGA/GIGA Kit (Genomed, Löhne, DE) according to the manufacturer's protocol. .. The quantity of isolated plasmid DNA was determined by a spectrophotometer at 260 nm (Epoch Microplate Spectrophotometer, Take3 Micro-Volume Plate, BioTek, Bad Friedrichshall, DE) and the quality by measuring the ratio of absorbance at A260 nm/280 nm and by agarose gel electrophoresis.

Isolation:

Article Title: Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma
Article Snippet: The expression of the shRNA molecule from the plasmid DNA in the cells was controlled by the human U6 promoter. .. The plasmid DNA was amplified in E. coli (TOP10; Invitrogen) and isolated using a NoEndo JETSTAR ENDOTOXIN-FREE MEGA/GIGA Kit (Genomed, Löhne, DE) according to the manufacturer's protocol. .. The quantity of isolated plasmid DNA was determined by a spectrophotometer at 260 nm (Epoch Microplate Spectrophotometer, Take3 Micro-Volume Plate, BioTek, Bad Friedrichshall, DE) and the quality by measuring the ratio of absorbance at A260 nm/280 nm and by agarose gel electrophoresis.

Pyrolysis Gas Chromatography:

Article Title: Characterization of a Salt-Tolerant Family 42 ?-Galactosidase from a Psychrophilic Antarctic Planococcus Isolate
Article Snippet: .. Plasmid DNA from one transformant was purified by using a Genomed maxi prep kit (PGC, Gaithersburg, Md.). .. The gene encoding the β-galactosidase activity was sequenced at the Penn State Nucleic Acid Facility with an ABI model 370 sequencer.

Article Title: Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein
Article Snippet: The Bac-rNV C8 (ORFs 2 and 3) used in these experiments was generated and described previously ( ). .. Plasmid DNA was linearized and purified on an agarose gel (Jetsorb, Genomed; PGC, Gaithersburg, Md.). .. The purified DNA was transcribed in vitro with SP6 or T7 polymerase using the MaxiScript kit (Ambion, Austin, Tex.).

Agarose Gel Electrophoresis:

Article Title: Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein
Article Snippet: The Bac-rNV C8 (ORFs 2 and 3) used in these experiments was generated and described previously ( ). .. Plasmid DNA was linearized and purified on an agarose gel (Jetsorb, Genomed; PGC, Gaithersburg, Md.). .. The purified DNA was transcribed in vitro with SP6 or T7 polymerase using the MaxiScript kit (Ambion, Austin, Tex.).

DNA Purification:

Article Title: Mutations Which Uncouple Transport and Phosphorylation in the d-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14
Article Snippet: All manipulations with recombinant DNA were performed by using standard procedures ( ). .. Plasmid DNA was prepared by the standard phenol extraction procedure ( ) or by using the JETstar DNA purification system (Genomed, Bad Oeynhausen, Germany). .. Restriction enzymes were purchased from New England Biolabs (Schwalbach, Germany) and were used according to the recommendations of the supplier.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Genomed GmbH plasmid dna preparation kit
    Plasmid Dna Preparation Kit, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna preparation kit/product/Genomed GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid dna preparation kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Genomed GmbH plasmid dna
    Plasmid Dna, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna/product/Genomed GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid dna - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results